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Protein

Oligoribonuclease

Gene

orn

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

3'-to-5' exoribonuclease specific for small oligoribonucleotides 2 to 5 nucleotides in length, as well as small (2 to 5 nucleotides) ssDNA oligomers. Probably responsible for the final step in mRNA degradation.3 Publications

Enzyme regulationi

Inhibited by adenosine 3',5'-bisphosphate (PAP).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Zinc1
Metal bindingi14Zinc1
Active sitei129Sequence analysis1
Metal bindingi163Zinc1

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro
  • oligoribonucleotidase activity Source: EcoCyc
  • single-stranded DNA 3'-5' exodeoxyribonuclease activity Source: EcoCyc

GO - Biological processi

  • RNA catabolic process Source: EcoCyc

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7842-MONOMER.
MetaCyc:G7842-MONOMER.
BRENDAi3.1.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligoribonuclease (EC:3.1.-.-)
Gene namesi
Name:orn
Synonyms:o204a, yjeR
Ordered Locus Names:b4162, JW5740
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12480. orn.

Subcellular locationi

P0A784:

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Essential. In depletion experiments the level of oligonucleotides is elevated and that of mononucleotides is severly reduced compared to wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001110332 – 181OligoribonucleaseAdd BLAST180

Proteomic databases

PaxDbiP0A784.
PRIDEiP0A784.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-10412N.
STRINGi316385.ECDH10B_4357.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi8 – 18Combined sources11
Turni20 – 22Combined sources3
Beta strandi25 – 33Combined sources9
Beta strandi39 – 47Combined sources9
Helixi52 – 55Combined sources4
Helixi60 – 68Combined sources9
Helixi71 – 77Combined sources7
Helixi82 – 93Combined sources12
Turni94 – 96Combined sources3
Beta strandi104 – 108Combined sources5
Helixi109 – 119Combined sources11
Helixi121 – 126Combined sources6
Beta strandi131 – 133Combined sources3
Helixi135 – 144Combined sources10
Helixi146 – 151Combined sources6
Helixi160 – 177Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YTAX-ray2.20A/B/C/D2-181[»]
2IGIX-ray1.70A/B2-181[»]
ProteinModelPortaliP0A784.
SMRiP0A784.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A784.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 171ExonucleaseAdd BLAST164

Sequence similaritiesi

Belongs to the oligoribonuclease family.Curated

Phylogenomic databases

eggNOGiENOG4108RBP. Bacteria.
COG1949. LUCA.
HOGENOMiHOG000246596.
InParanoidiP0A784.
KOiK13288.
PhylomeDBiP0A784.

Family and domain databases

CDDicd06135. Orn. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00045. Oligoribonuclease. 1 hit.
InterProiView protein in InterPro
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR022894. Oligoribonuclease.
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
PfamiView protein in Pfam
PF00929. RNase_T. 1 hit.
SMARTiView protein in SMART
SM00479. EXOIII. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANENNLIW IDLEMTGLDP ERDRIIEIAT LVTDANLNIL AEGPTIAVHQ
60 70 80 90 100
SDEQLALMDD WNVRTHTASG LVERVKASTM GDREAELATL EFLKQWVPAG
110 120 130 140 150
KSPICGNSIG QDRRFLFKYM PELEAYFHYR YLDVSTLKEL ARRWKPEILD
160 170 180
GFTKQGTHQA MDDIRESVAE LAYYREHFIK L
Length:181
Mass (Da):20,816
Last modified:January 23, 2007 - v2
Checksum:i57A5C7D500C4502F
GO

Sequence cautioni

P0A784: The sequence AAA97061 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97061.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77122.2.
AP009048 Genomic DNA. Translation: BAE78166.1.
PIRiS56390.
RefSeqiNP_418586.4. NC_000913.3.
WP_001295188.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77122; AAC77122; b4162.
BAE78166; BAE78166; BAE78166.
GeneIDi948675.
KEGGiecj:JW5740.
eco:b4162.
PATRICifig|1411691.4.peg.2536.

Similar proteinsi

Entry informationi

Entry nameiORN_ECOLI
AccessioniPrimary (citable) accession number: P0A784
Secondary accession number(s): P39287, P76799, Q2M6E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families