P0A784 (ORN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligoribonuclease
EC=3.1.-.-

Gene names

Name:	orn
Synonyms:	yjeR
Ordered Locus Names:	b4162, JW5740

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	3'-to-5' exoribonuclease specific for small oligoribonucleotides. HAMAP MF_00045
Subunit structure	Homodimer.
Subcellular location	Cytoplasm Potential HAMAP MF_00045.
Sequence similarities	Belongs to the oligoribonuclease family. Contains 1 exonuclease domain.
Sequence caution	The sequence AAA97061.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from mutant phenotype. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from direct assay Ref.4. Source: EcoCyc nucleic acid binding Inferred from electronic annotation. Source: InterPro oligoribonucleotidase activity Inferred from direct assay. Source: EcoCyc single-stranded DNA specific 3'-5' exodeoxyribonuclease activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 181

180

Oligoribonuclease HAMAP MF_00045

PRO_0000111033

Regions

Domain

8 – 171

164

Exonuclease

Sites

Active site

129

1

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

181

Helix Strand Turn

Details...

Beta strand

8 – 18

11

Turn

20 – 22

3

Beta strand

25 – 33

9

Beta strand

39 – 42

4

Helix

52 – 55

4

Helix

60 – 68

9

Helix

71 – 77

7

Helix

82 – 93

12

Turn

94 – 96

3

Beta strand

104 – 108

5

Helix

109 – 119

11

Helix

121 – 126

6

Beta strand

131 – 133

3

Helix

134 – 144

11

Helix

148 – 151

4

Helix

160 – 177

18

Sequences

	Sequence		Length	Mass (Da)
	P0A784 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 57A5C7D500C4502F	FASTA	181	20,816
	10 20 30 40 50 60 MSANENNLIW IDLEMTGLDP ERDRIIEIAT LVTDANLNIL AEGPTIAVHQ SDEQLALMDD 70 80 90 100 110 120 WNVRTHTASG LVERVKASTM GDREAELATL EFLKQWVPAG KSPICGNSIG QDRRFLFKYM 130 140 150 160 170 180 PELEAYFHYR YLDVSTLKEL ARRWKPEILD GFTKQGTHQA MDDIRESVAE LAYYREHFIK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily." Zhang X., Zhu L., Deutscher M.P. J. Bacteriol. 180:2779-2781(1998) [PubMed: 9573169] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U14003 Genomic DNA. Translation: AAA97061.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77122.2.
AP009048 Genomic DNA. Translation: BAE78166.1.

PIR

S56390.

RefSeq

NP_418586.4. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YTA	X-ray	2.20	A/B/C/D	1-181	[»]
2IGI	X-ray	1.70	A/B	2-180	[»]

ProteinModelPortal

P0A784.

SMR

P0A784. Positions 2-181.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002902; EBESCP00000002902; EBESCG00000002369.
EBESCT00000014870; EBESCP00000014161; EBESCG00000013930.

GeneID

948675.

GenomeReviews

Gene locus JW5740 in contig AP009048_GR.
Gene locus b4162 in contig U00096_GR.

KEGG

ecj:JW5740.
eco:b4162.

PATRIC

32123897. VBIEscCol129921_4296.

Organism-specific databases

EchoBASE

EB2373.

EcoGene

EG12480. orn.

Phylogenomic databases

eggNOG

COG1949.

GeneTree

EBGT00050000011032.

HOGENOM

HBG448545.

OMA

WNKGTHG.

PhylomeDB

P0A784.

ProtClustDB

PRK05359.

Enzyme and pathway databases

BioCyc

EcoCyc:G7842-MONOMER.
MetaCyc:G7842-MONOMER.

Gene expression databases

Genevestigator

P0A784.

Family and domain databases

HAMAP

MF_00045. Oligoribonuclease.
[Tree]

InterPro

IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR022894. Oligoribonuclease.
IPR012337. RNaseH-like_dom.
[Graphical view]

KO

K13288.

Pfam

PF00929. RNase_T. 1 hit.
[Graphical view]

SMART

SM00479. EXOIII. 1 hit.
[Graphical view]

SUPFAM

SSF53098. RNaseH_fold. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ORN_ECOLI

Accession

Primary (citable) accession number: P0A784
Secondary accession number(s): P39287, P76799, Q2M6E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program