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Protein

N utilization substance protein B

Gene

nusB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10666-MONOMER.
ECOL316407:JW0406-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N utilization substance protein B
Short name:
Protein NusB
Gene namesi
Name:nusB
Synonyms:ssyB
Ordered Locus Names:b0416, JW0406
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10666. nusB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139N utilization substance protein BPRO_0000176536Add
BLAST

Proteomic databases

EPDiP0A780.
PaxDbiP0A780.
PRIDEiP0A780.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsJP0A7R55EBI-555387,EBI-544602

Protein-protein interaction databases

BioGridi4259834. 29 interactions.
DIPiDIP-48254N.
IntActiP0A780. 12 interactions.
MINTiMINT-1220894.
STRINGi511145.b0416.

Structurei

Secondary structure

1
139
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2320Combined sources
Helixi27 – 3711Combined sources
Beta strandi40 – 423Combined sources
Helixi45 – 5713Combined sources
Helixi59 – 668Combined sources
Helixi67 – 704Combined sources
Helixi74 – 763Combined sources
Helixi79 – 9416Combined sources
Helixi100 – 11415Combined sources
Helixi119 – 13416Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EY1NMR-A1-139[»]
3D3BX-ray1.30A1-139[»]
3D3CX-ray2.60A/B/C1-139[»]
3IMQX-ray2.50A/B/C3-139[»]
ProteinModelPortaliP0A780.
SMRiP0A780. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A780.

Family & Domainsi

Sequence similaritiesi

Belongs to the NusB family.Curated

Phylogenomic databases

eggNOGiENOG4107YIM. Bacteria.
COG0781. LUCA.
HOGENOMiHOG000281868.
InParanoidiP0A780.
KOiK03625.
OMAiIPYKVVI.
PhylomeDBiP0A780.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
HAMAPiMF_00073. NusB. 1 hit.
InterProiIPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
[Graphical view]
PANTHERiPTHR11078. PTHR11078. 1 hit.
PfamiPF01029. NusB. 1 hit.
[Graphical view]
SUPFAMiSSF48013. SSF48013. 1 hit.
TIGRFAMsiTIGR01951. nusB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE
60 70 80 90 100
LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY
110 120 130
KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
Length:139
Mass (Da):15,689
Last modified:June 7, 2005 - v1
Checksum:i0BB912103061243B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591T → S (PubMed:6330693).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti18 – 181Y → D in nusB5; abolishes antitermination.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00681 Genomic DNA. Translation: CAA25289.1.
X64395 Genomic DNA. Translation: CAA45737.1.
M26839 Genomic DNA. Translation: AAA24228.1.
U82664 Genomic DNA. Translation: AAB40172.1.
U00096 Genomic DNA. Translation: AAC73519.1.
AP009048 Genomic DNA. Translation: BAE76196.1.
PIRiI51822. FJECB.
RefSeqiNP_414950.1. NC_000913.3.
WP_000801125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73519; AAC73519; b0416.
BAE76196; BAE76196; BAE76196.
GeneIDi945054.
KEGGiecj:JW0406.
eco:b0416.
PATRICi32115981. VBIEscCol129921_0432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00681 Genomic DNA. Translation: CAA25289.1.
X64395 Genomic DNA. Translation: CAA45737.1.
M26839 Genomic DNA. Translation: AAA24228.1.
U82664 Genomic DNA. Translation: AAB40172.1.
U00096 Genomic DNA. Translation: AAC73519.1.
AP009048 Genomic DNA. Translation: BAE76196.1.
PIRiI51822. FJECB.
RefSeqiNP_414950.1. NC_000913.3.
WP_000801125.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EY1NMR-A1-139[»]
3D3BX-ray1.30A1-139[»]
3D3CX-ray2.60A/B/C1-139[»]
3IMQX-ray2.50A/B/C3-139[»]
ProteinModelPortaliP0A780.
SMRiP0A780. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259834. 29 interactions.
DIPiDIP-48254N.
IntActiP0A780. 12 interactions.
MINTiMINT-1220894.
STRINGi511145.b0416.

Proteomic databases

EPDiP0A780.
PaxDbiP0A780.
PRIDEiP0A780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73519; AAC73519; b0416.
BAE76196; BAE76196; BAE76196.
GeneIDi945054.
KEGGiecj:JW0406.
eco:b0416.
PATRICi32115981. VBIEscCol129921_0432.

Organism-specific databases

EchoBASEiEB0660.
EcoGeneiEG10666. nusB.

Phylogenomic databases

eggNOGiENOG4107YIM. Bacteria.
COG0781. LUCA.
HOGENOMiHOG000281868.
InParanoidiP0A780.
KOiK03625.
OMAiIPYKVVI.
PhylomeDBiP0A780.

Enzyme and pathway databases

BioCyciEcoCyc:EG10666-MONOMER.
ECOL316407:JW0406-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A780.
PROiP0A780.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
HAMAPiMF_00073. NusB. 1 hit.
InterProiIPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
[Graphical view]
PANTHERiPTHR11078. PTHR11078. 1 hit.
PfamiPF01029. NusB. 1 hit.
[Graphical view]
SUPFAMiSSF48013. SSF48013. 1 hit.
TIGRFAMsiTIGR01951. nusB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUSB_ECOLI
AccessioniPrimary (citable) accession number: P0A780
Secondary accession number(s): P04381, Q2MC10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.