Reviewed, UniProtKB/Swiss-Prot P0A780 (NUSB_ECOLI)

Names and origin

Protein names

Recommended name:
    N utilization substance protein B
      Short name=Protein nusB

Gene names

Name:	nusB
Synonyms:	ssyB
Ordered Locus Names:	b0416, JW0406

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the boxA RNA motif. HAMAP MF_00073
Subunit structure	Monomer. HAMAP MF_00073
Sequence similarities	Belongs to the nusB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	RNA-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription termination Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription termination factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

N utilization substance protein B HAMAP MF_00073

PRO_0000176536

Natural variations

Natural variant

18

1

Y → D in nusB5; abolishes antitermination.

Experimental info

Sequence conflict

59

1

T → S Ref.1

Secondary structure

1

.

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.

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.

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139

Helix Strand Turn

Details...

Helix

9 – 23

15

Helix

28 – 36

9

Turn

37 – 39

3

Beta strand

40 – 42

3

Helix

46 – 66

21

Turn

74 – 76

3

Helix

79 – 93

15

Helix

101 – 110

10

Turn

111 – 115

5

Beta strand

117 – 120

4

Helix

121 – 134

14

Sequences

	Sequence		Length	Mass (Da)
	P0A780-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 0BB912103061243B	FASTA	139	15,689
	10 20 30 40 50 60 MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE LLAGVATNTA 70 80 90 100 110 120 YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY KVAINEAIEL AKSFGAEDSH 130 KFVNGVLDKA APVIRPNKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene." Swindle J., Ajioka J., Dawson D., Myers R., Carroll D., Georgopoulos C. Nucleic Acids Res. 12:4977-4985(1984) [PubMed: 6330693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and nucleotide sequencing of the nusB gene of E. coli." Ishii S., Hatada E., Maekawa T., Imamoto F. Nucleic Acids Res. 12:4987-4995(1984) [PubMed: 6330694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Escherichia coli proteins involved in regulation of transcription termination: function, structure, and expression of the nusA and nusB genes." Imamoto F., Nakamura Y. Adv. Biophys. 21:175-192(1986) [PubMed: 3019094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation." Taura T., Ueguchi C., Shiba K., Ito K. Mol. Gen. Genet. 234:429-432(1992) [PubMed: 1406588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[5]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein." Huenges M., Rolz C., Gschwind R., Peteranderl R., Berglechner F., Richter G., Bacher A., Kessler H., Gemmecker G. EMBO J. 17:4092-4100(1998) [PubMed: 9670024] [Abstract] Cited for: STRUCTURE BY NMR.
[9]	"The structure of the transcriptional antiterminator NusB from Escherichia coli." Altieri A.S., Mazzulla M.J., Horita D.A., Coats R.H., Wingfield P.T., Das A., Court D.L., Byrd R.A. Nat. Struct. Biol. 7:470-474(2000) [PubMed: 10881193] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X00681 Genomic DNA. Translation: CAA25289.1.
X64395 Genomic DNA. Translation: CAA45737.1.
M26839 Genomic DNA. Translation: AAA24228.1.
U82664 Genomic DNA. Translation: AAB40172.1.
U00096 Genomic DNA. Translation: AAC73519.1.
AP009048 Genomic DNA. Translation: BAE76196.1.

PIR

FJECB. I51822.

RefSeq

AP_001066.1.
NP_414950.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EY1	NMR	-	A	1-139	[»]
3D3B	X-ray	1.30	A	3-139	[»]
3D3C	X-ray	2.60	A/B/C	3-139	[»]
3IMQ	X-ray	2.50	A/B/C	3-139	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0A780.

2-D gel databases

ECO2DBASE

H013.8. 6TH EDITION.

Genome annotation databases

GeneID

945054.

GenomeReviews

Gene locus JW0406 in contig AP009048_GR.
Gene locus b0416 in contig U00096_GR.

KEGG

ecj:JW0406.
eco:b0416.

Organism-specific databases

EchoBASE

EB0660.

EcoGene

EG10666. nusB.

CMR

Search...

Phylogenomic databases

eggNOG

COG0781.

HOGENOM

HBG734409.

OMA

SHNDIAD.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10666-MONOMER.
ECOL168927:B0416-MONOMER.

Gene expression databases

Genevestigator

P0A780.

Family and domain databases

HAMAP

MF_00073. NusB.
[Tree]

InterPro

IPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
[Graphical view]

Gene3D

G3DSA:1.10.940.10. NusB_RsmB_TIM44. 1 hit.

Pfam

PF01029. NusB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01951. nusB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NUSB_ECOLI

Accession

Primary (citable) accession number: P0A780
Secondary accession number(s): P04381, Q2MC10

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)