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Protein

Transcription antitermination protein NusB

Gene

nusB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons (PubMed:7045592, PubMed:7678781, PubMed:11884128, PubMed:14973028, PubMed:15716433, PubMed:16109710). The affinity of NusB for the boxA RNA sequence is significantly increased in the presence of the ribosomal protein S10 (PubMed:16109710, PubMed:11884128). NusB may serve as a loading factor that ensures efficient entry of S10 into the transcription complexes (PubMed:19111659). It also modulates the rrn boxA-mediated transcription elongation rates (PubMed:10383769).8 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processTranscription, Transcription antitermination, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10666-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription antitermination protein NusBUniRule annotationCurated
Alternative name(s):
Antitermination factor NusB1 PublicationUniRule annotation
N utilization substance protein BCurated
Gene namesi
Name:nusB1 PublicationUniRule annotation
Synonyms:groNB1 Publication, ssyB1 Publication
Ordered Locus Names:b0416, JW0406
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10666. nusB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Inactivation of the gene causes cold-sensitive cell growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001765361 – 139Transcription antitermination protein NusBAdd BLAST139

Proteomic databases

PaxDbiP0A780.
PRIDEiP0A780.

Interactioni

Subunit structurei

Monomer in solution (PubMed:10881193, PubMed:16109710). Forms a heterodimer with the ribosomal protein S10 (PubMed:1731086, PubMed:11884128, PubMed:16109710, PubMed:19111659).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsJP0A7R56EBI-555387,EBI-544602

Protein-protein interaction databases

BioGridi4259834. 29 interactors.
DIPiDIP-48254N.
IntActiP0A780. 12 interactors.
MINTiMINT-1220894.
STRINGi316385.ECDH10B_0372.

Structurei

Secondary structure

1139
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 23Combined sources20
Helixi27 – 37Combined sources11
Beta strandi40 – 42Combined sources3
Helixi45 – 56Combined sources12
Helixi59 – 64Combined sources6
Helixi67 – 69Combined sources3
Turni70 – 72Combined sources3
Helixi74 – 76Combined sources3
Helixi79 – 94Combined sources16
Helixi100 – 114Combined sources15
Helixi119 – 134Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EY1NMR-A1-139[»]
3D3BX-ray1.30A1-139[»]
3D3CX-ray2.60A/B/C1-139[»]
3IMQX-ray2.50A/B/C3-139[»]
5MS0electron microscopy9.80L1-139[»]
ProteinModelPortaliP0A780.
SMRiP0A780.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A780.

Family & Domainsi

Domaini

The N-terminal region functions as an arginine-rich RNA-binding motif (ARM).1 Publication

Sequence similaritiesi

Belongs to the NusB family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4107YIM. Bacteria.
COG0781. LUCA.
HOGENOMiHOG000281868.
InParanoidiP0A780.
KOiK03625.
PhylomeDBiP0A780.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
HAMAPiMF_00073. NusB. 1 hit.
InterProiView protein in InterPro
IPR035926. NusB-like_sf.
IPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
PANTHERiPTHR11078. PTHR11078. 1 hit.
PfamiView protein in Pfam
PF01029. NusB. 1 hit.
SUPFAMiSSF48013. SSF48013. 1 hit.
TIGRFAMsiTIGR01951. nusB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE
60 70 80 90 100
LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY
110 120 130
KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
Length:139
Mass (Da):15,689
Last modified:June 7, 2005 - v1
Checksum:i0BB912103061243B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59T → S (PubMed:6330693).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti18Y → D in nusB5; abolishes antitermination. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00681 Genomic DNA. Translation: CAA25289.1.
X64395 Genomic DNA. Translation: CAA45737.1.
M26839 Genomic DNA. Translation: AAA24228.1.
U82664 Genomic DNA. Translation: AAB40172.1.
U00096 Genomic DNA. Translation: AAC73519.1.
AP009048 Genomic DNA. Translation: BAE76196.1.
PIRiI51822. FJECB.
RefSeqiNP_414950.1. NC_000913.3.
WP_000801125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73519; AAC73519; b0416.
BAE76196; BAE76196; BAE76196.
GeneIDi945054.
KEGGiecj:JW0406.
eco:b0416.
PATRICifig|1411691.4.peg.1861.

Similar proteinsi

Entry informationi

Entry nameiNUSB_ECOLI
AccessioniPrimary (citable) accession number: P0A780
Secondary accession number(s): P04381, Q2MC10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: June 7, 2005
Last modified: November 22, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families