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Protein

Transcription antitermination protein NusB

Gene

nusB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons (PubMed:7045592, PubMed:7678781, PubMed:11884128, PubMed:14973028, PubMed:15716433, PubMed:16109710). The affinity of NusB for the boxA RNA sequence is significantly increased in the presence of the ribosomal protein S10 (PubMed:16109710, PubMed:11884128). NusB may serve as a loading factor that ensures efficient entry of S10 into the transcription complexes (PubMed:19111659). It also modulates the rrn boxA-mediated transcription elongation rates (PubMed:10383769).8 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processTranscription, Transcription antitermination, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10666-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription antitermination protein NusBUniRule annotationCurated
Alternative name(s):
Antitermination factor NusB1 PublicationUniRule annotation
N utilization substance protein BCurated
Gene namesi
Name:nusB1 PublicationUniRule annotation
Synonyms:groNB1 Publication, ssyB1 Publication
Ordered Locus Names:b0416, JW0406
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10666 nusB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Inactivation of the gene causes cold-sensitive cell growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001765361 – 139Transcription antitermination protein NusBAdd BLAST139

Proteomic databases

EPDiP0A780
PaxDbiP0A780
PRIDEiP0A780

Interactioni

Subunit structurei

Monomer in solution (PubMed:10881193, PubMed:16109710). Forms a heterodimer with the ribosomal protein S10 (PubMed:1731086, PubMed:11884128, PubMed:16109710, PubMed:19111659).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsJP0A7R56EBI-555387,EBI-544602

Protein-protein interaction databases

BioGridi4259834, 56 interactors
DIPiDIP-48254N
IntActiP0A780, 12 interactors
STRINGi316385.ECDH10B_0372

Structurei

Secondary structure

1139
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 23Combined sources20
Helixi27 – 37Combined sources11
Beta strandi40 – 42Combined sources3
Helixi45 – 57Combined sources13
Helixi59 – 66Combined sources8
Helixi67 – 70Combined sources4
Helixi74 – 76Combined sources3
Helixi79 – 94Combined sources16
Helixi100 – 114Combined sources15
Helixi119 – 134Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EY1NMR-A1-139[»]
3D3BX-ray1.30A1-139[»]
3D3CX-ray2.60A/B/C1-139[»]
3IMQX-ray2.50A/B/C3-139[»]
5MS0electron microscopy9.80L1-139[»]
ProteinModelPortaliP0A780
SMRiP0A780
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A780

Family & Domainsi

Domaini

The N-terminal region functions as an arginine-rich RNA-binding motif (ARM).1 Publication

Sequence similaritiesi

Belongs to the NusB family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4107YIM Bacteria
COG0781 LUCA
HOGENOMiHOG000281868
InParanoidiP0A780
KOiK03625
OMAiIPYKVVI
PhylomeDBiP0A780

Family and domain databases

Gene3Di1.10.940.10, 1 hit
HAMAPiMF_00073 NusB, 1 hit
InterProiView protein in InterPro
IPR035926 NusB-like_sf
IPR011605 NusB_fam
IPR006027 NusB_RsmB_TIM44
PANTHERiPTHR11078 PTHR11078, 1 hit
PfamiView protein in Pfam
PF01029 NusB, 1 hit
SUPFAMiSSF48013 SSF48013, 1 hit
TIGRFAMsiTIGR01951 nusB, 1 hit

Sequencei

Sequence statusi: Complete.

P0A780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE
60 70 80 90 100
LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY
110 120 130
KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
Length:139
Mass (Da):15,689
Last modified:June 7, 2005 - v1
Checksum:i0BB912103061243B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59T → S (PubMed:6330693).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti18Y → D in nusB5; abolishes antitermination. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00681 Genomic DNA Translation: CAA25289.1
X64395 Genomic DNA Translation: CAA45737.1
M26839 Genomic DNA Translation: AAA24228.1
U82664 Genomic DNA Translation: AAB40172.1
U00096 Genomic DNA Translation: AAC73519.1
AP009048 Genomic DNA Translation: BAE76196.1
PIRiI51822 FJECB
RefSeqiNP_414950.1, NC_000913.3
WP_000801125.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73519; AAC73519; b0416
BAE76196; BAE76196; BAE76196
GeneIDi945054
KEGGiecj:JW0406
eco:b0416
PATRICifig|1411691.4.peg.1861

Similar proteinsi

Entry informationi

Entry nameiNUSB_ECOLI
AccessioniPrimary (citable) accession number: P0A780
Secondary accession number(s): P04381, Q2MC10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: June 7, 2005
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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