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Protein

Divalent metal cation transporter MntH

Gene

mntH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

H+-stimulated, divalent metal cation uptake system. Involved in manganese and iron uptake. Can also transport cadmium, cobalt, zinc and to a lesser extent nickel and copper. Involved in response to reactive oxygen.UniRule annotation2 Publications

GO - Molecular functioni

  • cadmium ion transmembrane transporter activity Source: EcoliWiki
  • hydrogen ion transmembrane transporter activity Source: EcoliWiki
  • manganese ion transmembrane transporter activity Source: EcoliWiki
  • metal ion binding Source: UniProtKB-HAMAP
  • symporter activity Source: EcoliWiki
  • uniporter activity Source: EcoliWiki

GO - Biological processi

  • cadmium ion transport Source: EcoliWiki
  • cobalt ion transport Source: EcoliWiki
  • ferrous iron transport Source: EcoliWiki
  • iron ion transport Source: EcoliWiki
  • manganese ion transport Source: EcoCyc
  • metal ion transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Ion transport, Symport, Transport

Keywords - Ligandi

Iron, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:YFEP-MONOMER.
ECOL316407:JW2388-MONOMER.
MetaCyc:YFEP-MONOMER.

Protein family/group databases

TCDBi2.A.55.3.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Divalent metal cation transporter MntHUniRule annotation
Gene namesi
Name:mntHUniRule annotation
Synonyms:yfeP
Ordered Locus Names:b2392, JW2388
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14157. mntH.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicUniRule annotationAdd
BLAST
Transmembranei20 – 3920HelicalUniRule annotationAdd
BLAST
Topological domaini40 – 5112PeriplasmicUniRule annotationAdd
BLAST
Transmembranei52 – 7120HelicalUniRule annotationAdd
BLAST
Topological domaini72 – 9524CytoplasmicUniRule annotationAdd
BLAST
Transmembranei96 – 11823HelicalUniRule annotationAdd
BLAST
Topological domaini119 – 1257PeriplasmicUniRule annotation
Transmembranei126 – 14520HelicalUniRule annotationAdd
BLAST
Topological domaini146 – 15510CytoplasmicUniRule annotation
Transmembranei156 – 17520HelicalUniRule annotationAdd
BLAST
Topological domaini176 – 19621PeriplasmicUniRule annotationAdd
BLAST
Transmembranei197 – 22024HelicalUniRule annotationAdd
BLAST
Topological domaini221 – 23818CytoplasmicUniRule annotationAdd
BLAST
Transmembranei239 – 25820HelicalUniRule annotationAdd
BLAST
Topological domaini259 – 27618PeriplasmicUniRule annotationAdd
BLAST
Transmembranei277 – 29721HelicalUniRule annotationAdd
BLAST
Topological domaini298 – 32730CytoplasmicUniRule annotationAdd
BLAST
Transmembranei328 – 34417HelicalUniRule annotationAdd
BLAST
Topological domaini345 – 3506PeriplasmicUniRule annotation
Transmembranei351 – 37020HelicalUniRule annotationAdd
BLAST
Topological domaini371 – 38717CytoplasmicUniRule annotationAdd
BLAST
Transmembranei388 – 40619HelicalUniRule annotationAdd
BLAST
Topological domaini407 – 4126PeriplasmicUniRule annotation

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341D → E or N: Loss of function. 1 Publication
Mutagenesisi35 – 351P → N or Q: Loss of function. 1 Publication
Mutagenesisi36 – 361G → P: Loss of function. 1 Publication
Mutagenesisi37 – 371N → D: Loss of function. 1 Publication
Mutagenesisi102 – 1021E → D or Q: Loss of function. 1 Publication
Mutagenesisi109 – 1091D → E or N: Transports manganese to a very low level. 1 Publication
Mutagenesisi112 – 1121E → D or Q: Transports manganese to a very low level. 1 Publication
Mutagenesisi115 – 1151G → A or P: Loss of function. 1 Publication
Mutagenesisi154 – 1541E → Q: Transports manganese to a level half of that of wild-type. 1 Publication
Mutagenesisi238 – 2381D → N: Transports manganese to a very low level. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Divalent metal cation transporter MntHPRO_0000212617Add
BLAST

Proteomic databases

PaxDbiP0A769.

Interactioni

Protein-protein interaction databases

BioGridi4259183. 8 interactions.
DIPiDIP-48004N.
IntActiP0A769. 1 interaction.
STRINGi511145.b2392.

Structurei

3D structure databases

ProteinModelPortaliP0A769.
SMRiP0A769. Positions 30-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NRAMP family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C56. Bacteria.
COG1914. LUCA.
HOGENOMiHOG000152205.
InParanoidiP0A769.
KOiK03322.
OMAiAFHFNGH.
OrthoDBiEOG654P21.
PhylomeDBiP0A769.

Family and domain databases

HAMAPiMF_00221. NRAMP.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNYRVESSS GRAARKMRLA LMGPAFIAAI GYIDPGNFAT NIQAGASFGY
60 70 80 90 100
QLLWVVVWAN LMAMLIQILS AKLGIATGKN LAEQIRDHYP RPVVWFYWVQ
110 120 130 140 150
AEIIAMATDL AEFIGAAIGF KLILGVSLLQ GAVLTGIATF LILMLQRRGQ
160 170 180 190 200
KPLEKVIGGL LLFVAAAYIV ELIFSQPNLA QLGKGMVIPS LPTSEAVFLA
210 220 230 240 250
AGVLGATIMP HVIYLHSSLT QHLHGGSRQQ RYSATKWDVA IAMTIAGFVN
260 270 280 290 300
LAMMATAAAA FHFSGHTGVA DLDEAYLTLQ PLLSHAAATV FGLSLVAAGL
310 320 330 340 350
SSTVVGTLAG QVVMQGFIRF HIPLWVRRTV TMLPSFIVIL MGLDPTRILV
360 370 380 390 400
MSQVLLSFGI ALALVPLLIF TSDSKLMGDL VNSKRVKQTG WVIVVLVVAL
410
NIWLLVGTAL GL
Length:412
Mass (Da):44,194
Last modified:June 7, 2005 - v1
Checksum:i7D77FEAEA00411FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161318 Genomic DNA. Translation: AAD46617.1.
U00096 Genomic DNA. Translation: AAC75451.1.
AP009048 Genomic DNA. Translation: BAA16262.1.
PIRiE65013.
RefSeqiNP_416893.1. NC_000913.3.
WP_000186369.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75451; AAC75451; b2392.
BAA16262; BAA16262; BAA16262.
GeneIDi946899.
KEGGiecj:JW2388.
eco:b2392.
PATRICi32120161. VBIEscCol129921_2489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161318 Genomic DNA. Translation: AAD46617.1.
U00096 Genomic DNA. Translation: AAC75451.1.
AP009048 Genomic DNA. Translation: BAA16262.1.
PIRiE65013.
RefSeqiNP_416893.1. NC_000913.3.
WP_000186369.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A769.
SMRiP0A769. Positions 30-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259183. 8 interactions.
DIPiDIP-48004N.
IntActiP0A769. 1 interaction.
STRINGi511145.b2392.

Protein family/group databases

TCDBi2.A.55.3.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Proteomic databases

PaxDbiP0A769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75451; AAC75451; b2392.
BAA16262; BAA16262; BAA16262.
GeneIDi946899.
KEGGiecj:JW2388.
eco:b2392.
PATRICi32120161. VBIEscCol129921_2489.

Organism-specific databases

EchoBASEiEB3909.
EcoGeneiEG14157. mntH.

Phylogenomic databases

eggNOGiENOG4105C56. Bacteria.
COG1914. LUCA.
HOGENOMiHOG000152205.
InParanoidiP0A769.
KOiK03322.
OMAiAFHFNGH.
OrthoDBiEOG654P21.
PhylomeDBiP0A769.

Enzyme and pathway databases

BioCyciEcoCyc:YFEP-MONOMER.
ECOL316407:JW2388-MONOMER.
MetaCyc:YFEP-MONOMER.

Miscellaneous databases

PROiP0A769.

Family and domain databases

HAMAPiMF_00221. NRAMP.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen."
    Kehres D.G., Zaharik M.L., Finlay B.B., Maguire M.E.
    Mol. Microbiol. 36:1085-1100(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12 / MM1955.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of the Escherichia coli K-12 Nramp orthologue (MntH) as a selective divalent metal ion transporter."
    Makui H., Roig E., Cole S.T., Helmann J.D., Gros P., Cellier M.F.M.
    Mol. Microbiol. 35:1065-1078(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  6. "Determination of transmembrane topology of the Escherichia coli natural resistance-associated macrophage protein (Nramp) ortholog."
    Courville P., Chaloupka R., Veyrier F., Cellier M.F.M.
    J. Biol. Chem. 279:3318-3326(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
  7. "Importance of conserved acidic residues in mntH, the Nramp homolog of Escherichia coli."
    Haemig H.A., Brooker R.J.
    J. Membr. Biol. 201:97-107(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-34; PRO-35; GLY-36; ASN-37; GLU-102; ASP-109; GLU-112; GLY-115; GLU-154 AND ASP-238.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMNTH_ECOLI
AccessioniPrimary (citable) accession number: P0A769
Secondary accession number(s): P77145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.