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Protein

Divalent metal cation transporter MntH

Gene

mntH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

H+-stimulated, divalent metal cation uptake system. Involved in manganese and iron uptake. Can also transport cadmium, cobalt, zinc and to a lesser extent nickel and copper. Involved in response to reactive oxygen.UniRule annotation2 Publications

GO - Molecular functioni

  • cadmium ion transmembrane transporter activity Source: EcoliWiki
  • hydrogen ion transmembrane transporter activity Source: EcoliWiki
  • manganese ion transmembrane transporter activity Source: EcoliWiki
  • metal ion binding Source: UniProtKB-HAMAP
  • symporter activity Source: EcoliWiki
  • uniporter activity Source: EcoliWiki

GO - Biological processi

  • cadmium ion transport Source: EcoliWiki
  • cobalt ion transport Source: EcoliWiki
  • ferrous iron transport Source: EcoliWiki
  • iron ion transport Source: EcoliWiki
  • manganese ion transport Source: EcoCyc
  • metal ion transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Ion transport, Symport, Transport

Keywords - Ligandi

Iron, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:YFEP-MONOMER.
ECOL316407:JW2388-MONOMER.
MetaCyc:YFEP-MONOMER.

Protein family/group databases

TCDBi2.A.55.3.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Divalent metal cation transporter MntHUniRule annotation
Gene namesi
Name:mntHUniRule annotation
Synonyms:yfeP
Ordered Locus Names:b2392, JW2388
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14157. mntH.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicUniRule annotationAdd BLAST19
Transmembranei20 – 39HelicalUniRule annotationAdd BLAST20
Topological domaini40 – 51PeriplasmicUniRule annotationAdd BLAST12
Transmembranei52 – 71HelicalUniRule annotationAdd BLAST20
Topological domaini72 – 95CytoplasmicUniRule annotationAdd BLAST24
Transmembranei96 – 118HelicalUniRule annotationAdd BLAST23
Topological domaini119 – 125PeriplasmicUniRule annotation7
Transmembranei126 – 145HelicalUniRule annotationAdd BLAST20
Topological domaini146 – 155CytoplasmicUniRule annotation10
Transmembranei156 – 175HelicalUniRule annotationAdd BLAST20
Topological domaini176 – 196PeriplasmicUniRule annotationAdd BLAST21
Transmembranei197 – 220HelicalUniRule annotationAdd BLAST24
Topological domaini221 – 238CytoplasmicUniRule annotationAdd BLAST18
Transmembranei239 – 258HelicalUniRule annotationAdd BLAST20
Topological domaini259 – 276PeriplasmicUniRule annotationAdd BLAST18
Transmembranei277 – 297HelicalUniRule annotationAdd BLAST21
Topological domaini298 – 327CytoplasmicUniRule annotationAdd BLAST30
Transmembranei328 – 344HelicalUniRule annotationAdd BLAST17
Topological domaini345 – 350PeriplasmicUniRule annotation6
Transmembranei351 – 370HelicalUniRule annotationAdd BLAST20
Topological domaini371 – 387CytoplasmicUniRule annotationAdd BLAST17
Transmembranei388 – 406HelicalUniRule annotationAdd BLAST19
Topological domaini407 – 412PeriplasmicUniRule annotation6

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34D → E or N: Loss of function. 1 Publication1
Mutagenesisi35P → N or Q: Loss of function. 1 Publication1
Mutagenesisi36G → P: Loss of function. 1 Publication1
Mutagenesisi37N → D: Loss of function. 1 Publication1
Mutagenesisi102E → D or Q: Loss of function. 1 Publication1
Mutagenesisi109D → E or N: Transports manganese to a very low level. 1 Publication1
Mutagenesisi112E → D or Q: Transports manganese to a very low level. 1 Publication1
Mutagenesisi115G → A or P: Loss of function. 1 Publication1
Mutagenesisi154E → Q: Transports manganese to a level half of that of wild-type. 1 Publication1
Mutagenesisi238D → N: Transports manganese to a very low level. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126171 – 412Divalent metal cation transporter MntHAdd BLAST412

Proteomic databases

PaxDbiP0A769.
PRIDEiP0A769.

Interactioni

Protein-protein interaction databases

BioGridi4259183. 8 interactors.
DIPiDIP-48004N.
IntActiP0A769. 1 interactor.
STRINGi511145.b2392.

Structurei

3D structure databases

ProteinModelPortaliP0A769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NRAMP family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C56. Bacteria.
COG1914. LUCA.
HOGENOMiHOG000152205.
InParanoidiP0A769.
KOiK03322.
OMAiERYSATK.
PhylomeDBiP0A769.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNYRVESSS GRAARKMRLA LMGPAFIAAI GYIDPGNFAT NIQAGASFGY
60 70 80 90 100
QLLWVVVWAN LMAMLIQILS AKLGIATGKN LAEQIRDHYP RPVVWFYWVQ
110 120 130 140 150
AEIIAMATDL AEFIGAAIGF KLILGVSLLQ GAVLTGIATF LILMLQRRGQ
160 170 180 190 200
KPLEKVIGGL LLFVAAAYIV ELIFSQPNLA QLGKGMVIPS LPTSEAVFLA
210 220 230 240 250
AGVLGATIMP HVIYLHSSLT QHLHGGSRQQ RYSATKWDVA IAMTIAGFVN
260 270 280 290 300
LAMMATAAAA FHFSGHTGVA DLDEAYLTLQ PLLSHAAATV FGLSLVAAGL
310 320 330 340 350
SSTVVGTLAG QVVMQGFIRF HIPLWVRRTV TMLPSFIVIL MGLDPTRILV
360 370 380 390 400
MSQVLLSFGI ALALVPLLIF TSDSKLMGDL VNSKRVKQTG WVIVVLVVAL
410
NIWLLVGTAL GL
Length:412
Mass (Da):44,194
Last modified:June 7, 2005 - v1
Checksum:i7D77FEAEA00411FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161318 Genomic DNA. Translation: AAD46617.1.
U00096 Genomic DNA. Translation: AAC75451.1.
AP009048 Genomic DNA. Translation: BAA16262.1.
PIRiE65013.
RefSeqiNP_416893.1. NC_000913.3.
WP_000186369.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75451; AAC75451; b2392.
BAA16262; BAA16262; BAA16262.
GeneIDi946899.
KEGGiecj:JW2388.
eco:b2392.
PATRICi32120161. VBIEscCol129921_2489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161318 Genomic DNA. Translation: AAD46617.1.
U00096 Genomic DNA. Translation: AAC75451.1.
AP009048 Genomic DNA. Translation: BAA16262.1.
PIRiE65013.
RefSeqiNP_416893.1. NC_000913.3.
WP_000186369.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A769.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259183. 8 interactors.
DIPiDIP-48004N.
IntActiP0A769. 1 interactor.
STRINGi511145.b2392.

Protein family/group databases

TCDBi2.A.55.3.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Proteomic databases

PaxDbiP0A769.
PRIDEiP0A769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75451; AAC75451; b2392.
BAA16262; BAA16262; BAA16262.
GeneIDi946899.
KEGGiecj:JW2388.
eco:b2392.
PATRICi32120161. VBIEscCol129921_2489.

Organism-specific databases

EchoBASEiEB3909.
EcoGeneiEG14157. mntH.

Phylogenomic databases

eggNOGiENOG4105C56. Bacteria.
COG1914. LUCA.
HOGENOMiHOG000152205.
InParanoidiP0A769.
KOiK03322.
OMAiERYSATK.
PhylomeDBiP0A769.

Enzyme and pathway databases

BioCyciEcoCyc:YFEP-MONOMER.
ECOL316407:JW2388-MONOMER.
MetaCyc:YFEP-MONOMER.

Miscellaneous databases

PROiP0A769.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNTH_ECOLI
AccessioniPrimary (citable) accession number: P0A769
Secondary accession number(s): P77145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.