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Protein

Putative N-acetylmannosamine-6-phosphate 2-epimerase

Gene

nanE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).Curated

Catalytic activityi

N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate.

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 3 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. N-acetylneuraminate lyase (nanA)
  2. N-acetylmannosamine kinase (nanK)
  3. Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)
  4. N-acetylglucosamine-6-phosphate deacetylase (nagA)
  5. Glucosamine-6-phosphate deaminase (nagB)
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

GO - Molecular functioni

  • N-acylglucosamine-6-phosphate 2-epimerase activity Source: EcoCyc

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • N-acetylmannosamine catabolic process Source: EcoCyc
  • N-acetylneuraminate catabolic process Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciEcoCyc:NANE-MONOMER.
UniPathwayiUPA00629; UER00682.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative N-acetylmannosamine-6-phosphate 2-epimerase (EC:5.1.3.9)
Alternative name(s):
ManNAc-6-P epimerase
Gene namesi
Name:nanE
Synonyms:yhcJ
Ordered Locus Names:b3223, JW3192
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12816. nanE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001797701 – 229Putative N-acetylmannosamine-6-phosphate 2-epimeraseAdd BLAST229

Proteomic databases

EPDiP0A761.
PaxDbiP0A761.
PRIDEiP0A761.

Expressioni

Inductioni

Negatively regulated by the transcriptional repressor NanR. Induced by N-acetylneuraminate, via inactivation of NanR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261228. 113 interactors.
DIPiDIP-48021N.
IntActiP0A761. 8 interactors.
MINTiMINT-1307633.
STRINGi316385.ECDH10B_3400.

Structurei

3D structure databases

ProteinModelPortaliP0A761.
SMRiP0A761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NanE family.Curated

Phylogenomic databases

eggNOGiENOG4105C9S. Bacteria.
COG3010. LUCA.
HOGENOMiHOG000236669.
InParanoidiP0A761.
KOiK01788.
PhylomeDBiP0A761.

Family and domain databases

CDDicd04729. NanE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01235. ManNAc6P_epimer. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
PfamiView protein in Pfam
PF04131. NanE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLAQLDQK IAANGGLIVS CQPVPDSPLD KPEIVAAMAL AAEQAGAVAI
60 70 80 90 100
RIEGVANLQA TRAVVSVPII GIVKRDLEDS PVRITAYIED VDALAQAGAD
110 120 130 140 150
IIAIDGTDRP RPVPVETLLA RIHHHGLLAM TDCSTPEDGL ACQKLGAEII
160 170 180 190 200
GTTLSGYTTP ETPEEPDLAL VKTLSDAGCR VIAEGRYNTP AQAADAMRHG
210 220
AWAVTVGSAI TRLEHICQWY NTAMKKAVL
Length:229
Mass (Da):24,074
Last modified:June 7, 2005 - v1
Checksum:iAB6CB3F36FEC0825
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58025.1.
U00096 Genomic DNA. Translation: AAC76255.1.
AP009048 Genomic DNA. Translation: BAE77266.1.
PIRiA65114.
RefSeqiNP_417690.1. NC_000913.3.
WP_000054239.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76255; AAC76255; b3223.
BAE77266; BAE77266; BAE77266.
GeneIDi947745.
KEGGiecj:JW3192.
eco:b3223.
PATRICifig|1411691.4.peg.3505.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNANE_ECOLI
AccessioniPrimary (citable) accession number: P0A761
Secondary accession number(s): P45426, Q2M8Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 5, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families