Reviewed, UniProtKB/Swiss-Prot P0A761 (NANE_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative N-acetylmannosamine-6-phosphate 2-epimerase
    EC=5.1.3.9
Alternative name(s):
    ManNAc-6-P epimerase

Gene names

Name:	nanE
Synonyms:	yhcJ
Ordered Locus Names:	b3223, JW3192

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) Potential.
Catalytic activity	N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate. HAMAP MF_01235
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 3/5. HAMAP MF_01235
Sequence similarities	Belongs to the nanE family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylmannosamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	N-acylglucosamine-6-phosphate 2-epimerase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
birA	P06709	1	EBI-561432,EBI-545740
dcyD	P76316	1	EBI-561432,EBI-562060
rplS	P0A7K6	1	EBI-561432,EBI-543891

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Putative N-acetylmannosamine-6-phosphate 2-epimerase HAMAP MF_01235		PRO_0000179770

Sequences

	Sequence		Length	Mass (Da)
	P0A761-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: AB6CB3F36FEC0825	FASTA	229	24,074
	10 20 30 40 50 60 MSLLAQLDQK IAANGGLIVS CQPVPDSPLD KPEIVAAMAL AAEQAGAVAI RIEGVANLQA 70 80 90 100 110 120 TRAVVSVPII GIVKRDLEDS PVRITAYIED VDALAQAGAD IIAIDGTDRP RPVPVETLLA 130 140 150 160 170 180 RIHHHGLLAM TDCSTPEDGL ACQKLGAEII GTTLSGYTTP ETPEEPDLAL VKTLSDAGCR 190 200 210 220 VIAEGRYNTP AQAADAMRHG AWAVTVGSAI TRLEHICQWY NTAMKKAVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli." Plumbridge J., Vimr E. J. Bacteriol. 181:47-54(1999) [PubMed: 9864311] [Abstract] Cited for: PUTATIVE FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA58025.1. U00096 Genomic DNA. Translation: AAC76255.1. AP009048 Genomic DNA. Translation: BAE77266.1.
PIR	A65114.
RefSeq	AP_003765.1. NP_417690.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A761. 8 interactions.
STRING	P0A761.
Genome annotation databases
GeneID	947745.
GenomeReviews	Gene locus JW3192 in contig AP009048_GR. Gene locus b3223 in contig U00096_GR.
KEGG	ecj:JW3192. eco:b3223.
Organism-specific databases
EchoBASE	EB2667.
EcoGene	EG12816. nanE.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A761.
OMA	EHIVSWF
Enzyme and pathway databases
BioCyc	EcoCyc:NANE-MON. ECOL168927:B3223-MON. MetaCyc:NANE-MON.
Gene expression databases
Genevestigator	P0A761.
Family and domain databases
HAMAP	MF_01235. [Tree]
InterPro	IPR007260. NanE. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF04131. NanE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NANE_ECOLI

Accession

Primary (citable) accession number: P0A761
Secondary accession number(s): P45426, Q2M8Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 24, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)