P0A754 (KEFF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione-regulated potassium-efflux system ancillary protein kefF

Gene names

Name:	kefF
Synonyms:	yabF
Ordered Locus Names:	b0046, JW0045

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for full activity of kefC. Ref.4
Miscellaneous	It is unlikely that kefF has oxidoreductase activity, it has probably evolved from its function as oxidoreductase to be regulator of kefC. HAMAP MF_01414
Sequence similarities	Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	potassium ion transport Inferred from electronic annotation. Source: InterPro
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 176

176

Glutathione-regulated potassium-efflux system ancillary protein kefF HAMAP MF_01414

PRO_0000071635

Secondary structure

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176

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Turn

11 – 13

3

Helix

16 – 25

10

Beta strand

31 – 34

4

Helix

35 – 38

4

Helix

46 – 54

9

Beta strand

57 – 64

8

Beta strand

67 – 69

3

Helix

72 – 81

10

Beta strand

99 – 105

7

Helix

111 – 113

3

Helix

122 – 125

4

Helix

126 – 134

9

Beta strand

143 – 145

3

Turn

153 – 155

3

Helix

160 – 172

13

Sequences

	Sequence		Length	Mass (Da)
	P0A754 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: F641E3952F4EFC41	FASTA	176	20,170
	10 20 30 40 50 60 MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV 70 80 90 100 110 120 WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG 130 140 150 160 170 FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 79 AND 123. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli." Miller S., Ness L.S., Wood C.M., Fox B.C., Booth I.R. J. Bacteriol. 182:6536-6540(2000) [PubMed: 11053405] [Abstract] Cited for: FUNCTION. Strain: Frag5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC73157.1.
AP009048 Genomic DNA. Translation: BAB96614.2.

PIR

F64725.

RefSeq

NP_414588.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EYW	X-ray	2.40	A/B	1-176	[»]
3L9W	X-ray	1.75	A/B	1-176	[»]
3L9X	X-ray	2.10	A/B	1-176	[»]

ProteinModelPortal

P0A754.

SMR

P0A754. Positions 1-174.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35822N.

IntAct

P0A754. 5 interactions.

Protein family/group databases

TCDB

2.A.37.1.1. monovalent cation:proton antiporter-2 (CPA2) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004854; EBESCP00000004854; EBESCG00000003959.
EBESCT00000016889; EBESCP00000016180; EBESCG00000015948.

GeneID

944767.

GenomeReviews

Gene locus JW0045 in contig AP009048_GR.
Gene locus b0046 in contig U00096_GR.

KEGG

ecj:JW0045.
eco:b0046.

PATRIC

32115191. VBIEscCol129921_0047.

Organism-specific databases

EchoBASE

EB1528.

EcoGene

EG11568. kefF.

Phylogenomic databases

eggNOG

COG2249.

GeneTree

EBGT00050000010021.

HOGENOM

HBG532247.

OMA

QAQARHY.

PhylomeDB

P0A754.

ProtClustDB

PRK00871.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11568-MONOMER.

Gene expression databases

Genevestigator

P0A754.

Family and domain databases

HAMAP

MF_01414. K-H-efflux_KefF.
[Tree]

InterPro

IPR003680. Flavodoxin_fold.
IPR023948. K_H_efflux_KefF.
[Graphical view]

KO

K11746.

Pfam

PF02525. Flavodoxin_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KEFF_ECOLI

Accession

Primary (citable) accession number: P0A754
Secondary accession number(s): P31577, P75629

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program