P0A754 (KEFF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione-regulated potassium-efflux system ancillary protein KefF

Gene names

Name:	kefF
Synonyms:	yabF
Ordered Locus Names:	b0046, JW0045

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for full activity of KefC. Ref.4
Miscellaneous	It is unlikely that KefF has oxidoreductase activity, it has probably evolved from its function as oxidoreductase to be regulator of KefC. HAMAP-Rule MF_01414
Sequence similarities	Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of ion transmembrane transporter activity Inferred from direct assay PubMed 21742892. Source: EcoCyc potassium ion transport Inferred from electronic annotation. Source: HAMAP
Molecular_function	FMN binding Inferred from direct assay PubMed 21742892. Source: EcoCyc NAD(P)H dehydrogenase (quinone) activity Inferred from direct assay PubMed 21742892. Source: EcoCyc electron carrier activity Inferred from mutant phenotype PubMed 17679694. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 176

176

Glutathione-regulated potassium-efflux system ancillary protein KefF HAMAP-Rule MF_01414

PRO_0000071635

Secondary structure

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176

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

11 – 13

3

Helix

16 – 25

10

Beta strand

27 – 34

8

Helix

35 – 38

4

Helix

46 – 54

9

Beta strand

57 – 64

8

Beta strand

67 – 69

3

Helix

72 – 81

10

Turn

84 – 86

3

Beta strand

87 – 89

3

Turn

94 – 97

4

Beta strand

99 – 105

7

Helix

110 – 113

4

Beta strand

116 – 118

3

Helix

121 – 125

5

Helix

126 – 134

9

Beta strand

143 – 145

3

Helix

148 – 150

3

Helix

153 – 172

20

Sequences

	Sequence		Length	Mass (Da)
	P0A754 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: F641E3952F4EFC41	FASTA	176	20,170
	10 20 30 40 50 60 MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV 70 80 90 100 110 120 WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG 130 140 150 160 170 FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 79 AND 123. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli." Miller S., Ness L.S., Wood C.M., Fox B.C., Booth I.R. J. Bacteriol. 182:6536-6540(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: Frag5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC73157.1.
AP009048 Genomic DNA. Translation: BAB96614.2.

PIR

F64725.

RefSeq

NP_414588.1. NC_000913.2.
YP_488352.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EYW	X-ray	2.40	A/B	1-176	[»]
3L9W	X-ray	1.75	A/B	1-176	[»]
3L9X	X-ray	2.10	A/B	1-176	[»]

ProteinModelPortal

P0A754.

SMR

P0A754. Positions 1-174.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35822N.

IntAct

P0A754. 5 interactions.

STRING

511145.b0046.

Protein family/group databases

TCDB

2.A.37.1.1. monovalent cation:proton antiporter-2 (CPA2) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73157; AAC73157; b0046.
BAB96614; BAB96614; BAB96614.

GeneID

12933207.
944767.

KEGG

ecj:Y75_p0046.
eco:b0046.

PATRIC

32115191. VBIEscCol129921_0047.

Organism-specific databases

EchoBASE

EB1528.

EcoGene

EG11568. kefF.

Phylogenomic databases

eggNOG

COG2249.

HOGENOM

HOG000149972.

KO

K11746.

OMA

QAQARHY.

ProtClustDB

PRK00871.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11568-MONOMER.
ECOL316407:JW0045-MONOMER.
MetaCyc:EG11568-MONOMER.

Gene expression databases

Genevestigator

P0A754.

Family and domain databases

HAMAP

MF_01414. K-H-efflux_KefF.

InterPro

IPR003680. Flavodoxin_fold.
IPR023948. K_H_efflux_KefF.
[Graphical view]

Pfam

PF02525. Flavodoxin_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A754.

Entry information

Entry name

KEFF_ECOLI

Accession

Primary (citable) accession number: P0A754
Secondary accession number(s): P31577, P75629

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program