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Protein

Glutathione-regulated potassium-efflux system ancillary protein KefF

Gene

kefF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC. Can use a wide range of substrates, including electrophilic quinones, and its function could be to reduce the redox toxicity of electrophilic quinones in parallel with acting as triggers for the KefC efflux system.UniRule annotation3 Publications

Catalytic activityi

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.UniRule annotation1 Publication

Cofactori

FMNUniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8FMNUniRule annotation2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 17FMNUniRule annotation2 Publications4
Nucleotide bindingi65 – 68FMNUniRule annotation2 Publications4
Nucleotide bindingi105 – 108FMNUniRule annotation2 Publications4

GO - Molecular functioni

  • electron carrier activity Source: EcoliWiki
  • FMN binding Source: EcoCyc
  • NAD(P)H dehydrogenase (quinone) activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD

Enzyme and pathway databases

BioCyciEcoCyc:EG11568-MONOMER.
ECOL316407:JW0045-MONOMER.
MetaCyc:EG11568-MONOMER.

Protein family/group databases

TCDBi2.A.37.1.1. the monovalent cation:proton antiporter-2 (cpa2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-regulated potassium-efflux system ancillary protein KefFUniRule annotation
Alternative name(s):
Quinone oxidoreductase KefFUniRule annotation (EC:1.6.5.2UniRule annotation)
Gene namesi
Name:kefFUniRule annotation
Synonyms:yabF
Ordered Locus Names:b0046, JW0045
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11568. kefF.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41D → K: Strongly reduced potassium efflux. 1 Publication1
Mutagenesisi107G → S: Does not bind FMN. Lacks oxidoreductase activity, but is still able to activate potassium efflux. 1 Publication1
Mutagenesisi112H → W: Lacks oxidoreductase activity, but is still able to activate potassium efflux. 1 Publication1
Mutagenesisi149F → W: Lacks oxidoreductase activity, but is still able to activate potassium efflux. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000716351 – 176Glutathione-regulated potassium-efflux system ancillary protein KefFAdd BLAST176

Proteomic databases

PaxDbiP0A754.
PRIDEiP0A754.

Interactioni

Subunit structurei

Homodimer. Interacts with KefC, forming a heterotetramer with 2:2 stoichiometry.UniRule annotation4 Publications

Protein-protein interaction databases

BioGridi4262201. 9 interactors.
DIPiDIP-35822N.
IntActiP0A754. 6 interactors.
STRINGi511145.b0046.

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi11 – 13Combined sources3
Helixi16 – 25Combined sources10
Beta strandi27 – 34Combined sources8
Helixi35 – 38Combined sources4
Helixi46 – 54Combined sources9
Beta strandi57 – 64Combined sources8
Beta strandi67 – 69Combined sources3
Helixi72 – 81Combined sources10
Turni84 – 86Combined sources3
Beta strandi87 – 89Combined sources3
Turni94 – 97Combined sources4
Beta strandi99 – 105Combined sources7
Helixi110 – 113Combined sources4
Beta strandi116 – 118Combined sources3
Helixi121 – 125Combined sources5
Helixi126 – 134Combined sources9
Beta strandi143 – 145Combined sources3
Helixi148 – 150Combined sources3
Helixi153 – 172Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EYWX-ray2.40A/B1-176[»]
3L9WX-ray1.75A/B1-176[»]
3L9XX-ray2.10A/B1-176[»]
ProteinModelPortaliP0A754.
SMRiP0A754.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A754.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108MXQ. Bacteria.
COG2249. LUCA.
HOGENOMiHOG000149972.
InParanoidiP0A754.
KOiK11746.
OMAiVNADTEH.
PhylomeDBiP0A754.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
HAMAPiMF_01414. K_H_efflux_KefF. 1 hit.
InterProiIPR003680. Flavodoxin_fold.
IPR029039. Flavoprotein-like_dom.
IPR023948. K_H_efflux_KefF.
[Graphical view]
PfamiPF02525. Flavodoxin_2. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ
60 70 80 90 100
EALSRADLIV WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL
110 120 130 140 150
LWAVTTGGGE SHFEIGAHPG FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI
160 170
CDDETLEGQA RHYKQRLLEW QEAHHG
Length:176
Mass (Da):20,170
Last modified:June 21, 2005 - v1
Checksum:iF641E3952F4EFC41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73157.1.
AP009048 Genomic DNA. Translation: BAB96614.2.
PIRiF64725.
RefSeqiNP_414588.1. NC_000913.3.
WP_000600725.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73157; AAC73157; b0046.
BAB96614; BAB96614; BAB96614.
GeneIDi944767.
KEGGiecj:JW0045.
eco:b0046.
PATRICi32115191. VBIEscCol129921_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73157.1.
AP009048 Genomic DNA. Translation: BAB96614.2.
PIRiF64725.
RefSeqiNP_414588.1. NC_000913.3.
WP_000600725.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EYWX-ray2.40A/B1-176[»]
3L9WX-ray1.75A/B1-176[»]
3L9XX-ray2.10A/B1-176[»]
ProteinModelPortaliP0A754.
SMRiP0A754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262201. 9 interactors.
DIPiDIP-35822N.
IntActiP0A754. 6 interactors.
STRINGi511145.b0046.

Protein family/group databases

TCDBi2.A.37.1.1. the monovalent cation:proton antiporter-2 (cpa2) family.

Proteomic databases

PaxDbiP0A754.
PRIDEiP0A754.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73157; AAC73157; b0046.
BAB96614; BAB96614; BAB96614.
GeneIDi944767.
KEGGiecj:JW0045.
eco:b0046.
PATRICi32115191. VBIEscCol129921_0047.

Organism-specific databases

EchoBASEiEB1528.
EcoGeneiEG11568. kefF.

Phylogenomic databases

eggNOGiENOG4108MXQ. Bacteria.
COG2249. LUCA.
HOGENOMiHOG000149972.
InParanoidiP0A754.
KOiK11746.
OMAiVNADTEH.
PhylomeDBiP0A754.

Enzyme and pathway databases

BioCyciEcoCyc:EG11568-MONOMER.
ECOL316407:JW0045-MONOMER.
MetaCyc:EG11568-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A754.
PROiP0A754.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
HAMAPiMF_01414. K_H_efflux_KefF. 1 hit.
InterProiIPR003680. Flavodoxin_fold.
IPR029039. Flavoprotein-like_dom.
IPR023948. K_H_efflux_KefF.
[Graphical view]
PfamiPF02525. Flavodoxin_2. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKEFF_ECOLI
AccessioniPrimary (citable) accession number: P0A754
Secondary accession number(s): P31577, P75629
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.