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Protein

Nicotinate-nucleotide adenylyltransferase

Gene

nadD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).1 Publication

Catalytic activityi

ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD+.1 Publication

Enzyme regulationi

Activity is susceptible to product inhibition.1 Publication

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide adenylyltransferase (nadD)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  • NAD salvage Source: EcoCyc

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processPyridine nucleotide biosynthesis
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NICONUCADENYLYLTRAN-MONOMER
MetaCyc:NICONUCADENYLYLTRAN-MONOMER
BRENDAi2.7.7.1 2026
2.7.7.18 2026
UniPathwayiUPA00253; UER00332

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide adenylyltransferase (EC:2.7.7.181 Publication)
Alternative name(s):
Deamido-NAD(+) diphosphorylase
Deamido-NAD(+) pyrophosphorylase
Nicotinate mononucleotide adenylyltransferase
Short name:
NaMN adenylyltransferase
Gene namesi
Name:nadD
Synonyms:ybeN
Ordered Locus Names:b0639, JW0634
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13241 nadD

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638351

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001814071 – 213Nicotinate-nucleotide adenylyltransferaseAdd BLAST213

Proteomic databases

PaxDbiP0A752
PRIDEiP0A752

Interactioni

Protein-protein interaction databases

IntActiP0A752, 7 interactors
STRINGi316385.ECDH10B_0708

Chemistry databases

BindingDBiP0A752

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 30Combined sources14
Beta strandi35 – 39Combined sources5
Helixi53 – 64Combined sources12
Beta strandi70 – 72Combined sources3
Helixi75 – 78Combined sources4
Helixi85 – 96Combined sources12
Beta strandi102 – 107Combined sources6
Helixi108 – 113Combined sources6
Helixi114 – 116Combined sources3
Helixi120 – 126Combined sources7
Beta strandi128 – 132Combined sources5
Helixi144 – 153Combined sources10
Beta strandi154 – 156Combined sources3
Helixi160 – 163Combined sources4
Beta strandi164 – 166Combined sources3
Beta strandi169 – 172Combined sources4
Helixi181 – 189Combined sources9
Turni195 – 197Combined sources3
Helixi200 – 209Combined sources10
Turni210 – 212Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K4KX-ray2.00A/B/C/D1-213[»]
1K4MX-ray1.90A/B/C1-213[»]
ProteinModelPortaliP0A752
SMRiP0A752
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A752

Family & Domainsi

Sequence similaritiesi

Belongs to the NadD family.Curated

Phylogenomic databases

eggNOGiENOG4108Z1W Bacteria
COG1057 LUCA
HOGENOMiHOG000262781
InParanoidiP0A752
KOiK00969
OMAiIHIGHLI
PhylomeDBiP0A752

Family and domain databases

CDDicd02165 NMNAT, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00244 NaMN_adenylyltr, 1 hit
InterProiView protein in InterPro
IPR004821 Cyt_trans-like
IPR005248 NadD/NMNAT
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit
TIGR00482 TIGR00482, 1 hit

Sequencei

Sequence statusi: Complete.

P0A752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLQALFGG TFDPVHYGHL KPVETLANLI GLTRVTIIPN NVPPHRPQPE
60 70 80 90 100
ANSVQRKHML ELAIADKPLF TLDERELKRN APSYTAQTLK EWRQEQGPDV
110 120 130 140 150
PLAFIIGQDS LLTFPTWYEY ETILDNAHLI VCRRPGYPLE MAQPQYQQWL
160 170 180 190 200
EDHLTHNPED LHLQPAGKIY LAETPWFNIS ATIIRERLQN GESCEDLLPE
210
PVLTYINQQG LYR
Length:213
Mass (Da):24,528
Last modified:June 7, 2005 - v1
Checksum:iEA2C675282400CF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23163 Genomic DNA Translation: AAA64852.1
U82598 Genomic DNA Translation: AAB40840.1
U00096 Genomic DNA Translation: AAC73740.1
AP009048 Genomic DNA Translation: BAA35286.1
PIRiE64798
RefSeqiNP_415172.1, NC_000913.3
WP_000838889.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73740; AAC73740; b0639
BAA35286; BAA35286; BAA35286
GeneIDi945248
KEGGiecj:JW0634
eco:b0639
PATRICifig|1411691.4.peg.1629

Similar proteinsi

Entry informationi

Entry nameiNADD_ECOLI
AccessioniPrimary (citable) accession number: P0A752
Secondary accession number(s): P52085
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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