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Protein

Nicotinate-nucleotide adenylyltransferase

Gene

nadD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).

Catalytic activityi

ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD+.

Enzyme regulationi

Activity is susceptible to product inhibition.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide adenylyltransferase (nadD)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  • NAD salvage Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NICONUCADENYLYLTRAN-MONOMER.
ECOL316407:JW0634-MONOMER.
MetaCyc:NICONUCADENYLYLTRAN-MONOMER.
BRENDAi2.7.7.1. 2026.
2.7.7.18. 2026.
UniPathwayiUPA00253; UER00332.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide adenylyltransferase (EC:2.7.7.18)
Alternative name(s):
Deamido-NAD(+) diphosphorylase
Deamido-NAD(+) pyrophosphorylase
Nicotinate mononucleotide adenylyltransferase
Short name:
NaMN adenylyltransferase
Gene namesi
Name:nadD
Synonyms:ybeN
Ordered Locus Names:b0639, JW0634
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13241. nadD.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638351.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001814071 – 213Nicotinate-nucleotide adenylyltransferaseAdd BLAST213

Proteomic databases

PaxDbiP0A752.
PRIDEiP0A752.

Interactioni

Protein-protein interaction databases

IntActiP0A752. 7 interactors.
STRINGi511145.b0639.

Chemistry databases

BindingDBiP0A752.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 30Combined sources14
Beta strandi35 – 39Combined sources5
Helixi53 – 64Combined sources12
Beta strandi70 – 72Combined sources3
Helixi75 – 78Combined sources4
Helixi85 – 96Combined sources12
Beta strandi102 – 107Combined sources6
Helixi108 – 113Combined sources6
Helixi114 – 116Combined sources3
Helixi120 – 126Combined sources7
Beta strandi128 – 132Combined sources5
Helixi144 – 153Combined sources10
Beta strandi154 – 156Combined sources3
Helixi160 – 163Combined sources4
Beta strandi164 – 166Combined sources3
Beta strandi169 – 172Combined sources4
Helixi181 – 189Combined sources9
Turni195 – 197Combined sources3
Helixi200 – 209Combined sources10
Turni210 – 212Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K4KX-ray2.00A/B/C/D1-213[»]
1K4MX-ray1.90A/B/C1-213[»]
ProteinModelPortaliP0A752.
SMRiP0A752.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A752.

Family & Domainsi

Sequence similaritiesi

Belongs to the NadD family.Curated

Phylogenomic databases

eggNOGiENOG4108Z1W. Bacteria.
COG1057. LUCA.
HOGENOMiHOG000262781.
InParanoidiP0A752.
KOiK00969.
OMAiFLLLGWD.
PhylomeDBiP0A752.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00244. NaMN_adenylyltr. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR005248. NAMN_adtrnsfrase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR12039. PTHR12039. 1 hit.
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR00482. TIGR00482. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLQALFGG TFDPVHYGHL KPVETLANLI GLTRVTIIPN NVPPHRPQPE
60 70 80 90 100
ANSVQRKHML ELAIADKPLF TLDERELKRN APSYTAQTLK EWRQEQGPDV
110 120 130 140 150
PLAFIIGQDS LLTFPTWYEY ETILDNAHLI VCRRPGYPLE MAQPQYQQWL
160 170 180 190 200
EDHLTHNPED LHLQPAGKIY LAETPWFNIS ATIIRERLQN GESCEDLLPE
210
PVLTYINQQG LYR
Length:213
Mass (Da):24,528
Last modified:June 7, 2005 - v1
Checksum:iEA2C675282400CF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23163 Genomic DNA. Translation: AAA64852.1.
U82598 Genomic DNA. Translation: AAB40840.1.
U00096 Genomic DNA. Translation: AAC73740.1.
AP009048 Genomic DNA. Translation: BAA35286.1.
PIRiE64798.
RefSeqiNP_415172.1. NC_000913.3.
WP_000838889.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73740; AAC73740; b0639.
BAA35286; BAA35286; BAA35286.
GeneIDi945248.
KEGGiecj:JW0634.
eco:b0639.
PATRICi32116461. VBIEscCol129921_0670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23163 Genomic DNA. Translation: AAA64852.1.
U82598 Genomic DNA. Translation: AAB40840.1.
U00096 Genomic DNA. Translation: AAC73740.1.
AP009048 Genomic DNA. Translation: BAA35286.1.
PIRiE64798.
RefSeqiNP_415172.1. NC_000913.3.
WP_000838889.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K4KX-ray2.00A/B/C/D1-213[»]
1K4MX-ray1.90A/B/C1-213[»]
ProteinModelPortaliP0A752.
SMRiP0A752.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0A752. 7 interactors.
STRINGi511145.b0639.

Chemistry databases

BindingDBiP0A752.
ChEMBLiCHEMBL3638351.

Proteomic databases

PaxDbiP0A752.
PRIDEiP0A752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73740; AAC73740; b0639.
BAA35286; BAA35286; BAA35286.
GeneIDi945248.
KEGGiecj:JW0634.
eco:b0639.
PATRICi32116461. VBIEscCol129921_0670.

Organism-specific databases

EchoBASEiEB3030.
EcoGeneiEG13241. nadD.

Phylogenomic databases

eggNOGiENOG4108Z1W. Bacteria.
COG1057. LUCA.
HOGENOMiHOG000262781.
InParanoidiP0A752.
KOiK00969.
OMAiFLLLGWD.
PhylomeDBiP0A752.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00332.
BioCyciEcoCyc:NICONUCADENYLYLTRAN-MONOMER.
ECOL316407:JW0634-MONOMER.
MetaCyc:NICONUCADENYLYLTRAN-MONOMER.
BRENDAi2.7.7.1. 2026.
2.7.7.18. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A752.
PROiP0A752.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00244. NaMN_adenylyltr. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR005248. NAMN_adtrnsfrase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR12039. PTHR12039. 1 hit.
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR00482. TIGR00482. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADD_ECOLI
AccessioniPrimary (citable) accession number: P0A752
Secondary accession number(s): P52085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.