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Protein

Peptide methionine sulfoxide reductase MsrA

Gene

msrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.

Catalytic activityi

Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin.
L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52Cysteine sulfenic acid (-SOH) intermediate1

GO - Molecular functioni

  • L-methionine-(S)-S-oxide reductase activity Source: EcoCyc
  • oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor Source: EcoCyc
  • peptide-methionine (S)-S-oxide reductase activity Source: EcoCyc

GO - Biological processi

  • cellular protein modification process Source: UniProtKB-HAMAP
  • protein repair Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.
BRENDAi1.8.4.11. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide methionine sulfoxide reductase MsrA (EC:1.8.4.11)
Short name:
Protein-methionine-S-oxide reductase
Alternative name(s):
Peptide-methionine (S)-S-oxide reductase
Short name:
Peptide Met(O) reductase
Gene namesi
Name:msrA
Synonyms:pms
Ordered Locus Names:b4219, JW4178
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11433. msrA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52C → S: Loss of activity. 1 Publication1
Mutagenesisi87C → S: No effect. 1 Publication1
Mutagenesisi199C → S: Decrease in activity. 1 Publication1
Mutagenesisi207C → S: Decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001385462 – 212Peptide methionine sulfoxide reductase MsrAAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 199Redox-active; alternateCurated
Disulfide bondi199 ↔ 207Redox-active; alternateCurated

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0A744.
PaxDbiP0A744.
PRIDEiP0A744.

Interactioni

Protein-protein interaction databases

BioGridi4259307. 6 interactors.
IntActiP0A744. 4 interactors.
STRINGi511145.b4219.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Turni30 – 32Combined sources3
Beta strandi35 – 37Combined sources3
Beta strandi44 – 49Combined sources6
Helixi53 – 61Combined sources9
Beta strandi66 – 77Combined sources12
Helixi83 – 88Combined sources6
Beta strandi89 – 91Combined sources3
Beta strandi94 – 101Combined sources8
Turni103 – 105Combined sources3
Helixi108 – 117Combined sources10
Beta strandi123 – 127Combined sources5
Beta strandi130 – 132Combined sources3
Helixi133 – 135Combined sources3
Beta strandi138 – 140Combined sources3
Helixi144 – 163Combined sources20
Beta strandi180 – 182Combined sources3
Helixi185 – 187Combined sources3
Helixi190 – 193Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FF3X-ray1.90A/B/C2-212[»]
2GT3NMR-A1-212[»]
2IEMNMR-A2-212[»]
ProteinModelPortaliP0A744.
SMRiP0A744.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A744.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4107QXP. Bacteria.
COG0225. LUCA.
HOGENOMiHOG000263862.
InParanoidiP0A744.
KOiK07304.
OMAiMVLRSEI.
PhylomeDBiP0A744.

Family and domain databases

Gene3Di3.30.1060.10. 1 hit.
HAMAPiMF_01401. MsrA. 1 hit.
InterProiIPR028427. Met_Sox_Rdtase.
IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view]
PANTHERiPTHR10173. PTHR10173. 1 hit.
PfamiPF01625. PMSR. 1 hit.
[Graphical view]
SUPFAMiSSF55068. SSF55068. 1 hit.
TIGRFAMsiTIGR00401. msrA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM
60 70 80 90 100
GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV
110 120 130 140 150
YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA
160 170 180 190 200
RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG
210
IGGIGVCLPP EA
Length:212
Mass (Da):23,315
Last modified:January 23, 2007 - v2
Checksum:iB9DC86DC1203BD32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89992 Genomic DNA. Translation: AAA24399.1.
U14003 Genomic DNA. Translation: AAA97115.1.
U00096 Genomic DNA. Translation: AAC77176.1.
AP009048 Genomic DNA. Translation: BAE78220.1.
PIRiS56444.
RefSeqiNP_418640.1. NC_000913.3.
WP_001295196.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77176; AAC77176; b4219.
BAE78220; BAE78220; BAE78220.
GeneIDi948734.
KEGGiecj:JW4178.
eco:b4219.
PATRICi32124013. VBIEscCol129921_4351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89992 Genomic DNA. Translation: AAA24399.1.
U14003 Genomic DNA. Translation: AAA97115.1.
U00096 Genomic DNA. Translation: AAC77176.1.
AP009048 Genomic DNA. Translation: BAE78220.1.
PIRiS56444.
RefSeqiNP_418640.1. NC_000913.3.
WP_001295196.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FF3X-ray1.90A/B/C2-212[»]
2GT3NMR-A1-212[»]
2IEMNMR-A2-212[»]
ProteinModelPortaliP0A744.
SMRiP0A744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259307. 6 interactors.
IntActiP0A744. 4 interactors.
STRINGi511145.b4219.

Proteomic databases

EPDiP0A744.
PaxDbiP0A744.
PRIDEiP0A744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77176; AAC77176; b4219.
BAE78220; BAE78220; BAE78220.
GeneIDi948734.
KEGGiecj:JW4178.
eco:b4219.
PATRICi32124013. VBIEscCol129921_4351.

Organism-specific databases

EchoBASEiEB1403.
EcoGeneiEG11433. msrA.

Phylogenomic databases

eggNOGiENOG4107QXP. Bacteria.
COG0225. LUCA.
HOGENOMiHOG000263862.
InParanoidiP0A744.
KOiK07304.
OMAiMVLRSEI.
PhylomeDBiP0A744.

Enzyme and pathway databases

BioCyciEcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.
BRENDAi1.8.4.11. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A744.
PROiP0A744.

Family and domain databases

Gene3Di3.30.1060.10. 1 hit.
HAMAPiMF_01401. MsrA. 1 hit.
InterProiIPR028427. Met_Sox_Rdtase.
IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view]
PANTHERiPTHR10173. PTHR10173. 1 hit.
PfamiPF01625. PMSR. 1 hit.
[Graphical view]
SUPFAMiSSF55068. SSF55068. 1 hit.
TIGRFAMsiTIGR00401. msrA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSRA_ECOLI
AccessioniPrimary (citable) accession number: P0A744
Secondary accession number(s): P27110, Q2M686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.