Peptide methionine sulfoxide reductase MsrA - Escherichia coli (strain K12)

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P0A744 (MSRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peptide methionine sulfoxide reductase MsrA
Short name=Protein-methionine-S-oxide reductase
EC=1.8.4.11

Alternative name(s):
Peptide-methionine (S)-S-oxide reductase
Short name=Peptide Met(O) reductase

Gene names

Name:	msrA
Synonyms:	pms
Ordered Locus Names:	b4219, JW4178

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. HAMAP-Rule MF_01401

Catalytic activity

Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP-Rule MF_01401

L-methionine + thioredoxin disulfide + H₂O = L-methionine (S)-S-oxide + thioredoxin. HAMAP-Rule MF_01401

Sequence similarities

Belongs to the MsrA Met sulfoxide reductase family.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytosol Inferred from direct assay PubMed 17272300. Source: EcoCyc
Molecular_function	peptide-methionine (S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lrp	P0ACJ0	1	EBI-1129240,EBI-1113316

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1

Chain

2 – 212

211

Peptide methionine sulfoxide reductase MsrA HAMAP-Rule MF_01401

PRO_0000138546

Sites

Active site

Cysteine sulfenic acid (-SOH) intermediate

Amino acid modifications

Disulfide bond

52 ↔ 199

Redox-active; alternate Probable

Disulfide bond

199 ↔ 207

Redox-active; alternate Probable

Experimental info

Mutagenesis

C → S: Loss of activity. Ref.5

Mutagenesis

C → S: No effect. Ref.5

Mutagenesis

199

C → S: Decrease in activity. Ref.5

Mutagenesis

207

C → S: Decrease in activity. Ref.5

Secondary structure

212

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A744 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B9DC86DC1203BD32
Show »

FASTA

212

23,315

        10         20         30         40         50         60 
MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF 

        70         80         90        100        110        120 
WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD 

       130        140        150        160        170        180 
PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF 

       190        200        210 
YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene." Rahman M.A., Nelson H., Weissbach H., Brot N. J. Biol. Chem. 267:15549-15551(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-30. Strain: B.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli." Boschi-Muller S., Azza S., Sanglier-Cianferani S., Talfournier F., van Dorsselaer A., Branlant G. J. Biol. Chem. 275:35908-35913(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-52; CYS-87; CYS-199 AND CYS-207.
[6]	"Crystal structure of the Escherichia coli peptide methionine sulphoxide reductase at 1.9-A resolution." Tete-Favier F., Cobessi D., Boschi-Muller S., Azza S., Branlant G., Aubry A. Structure 8:1167-1178(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M89992 Genomic DNA. Translation: AAA24399.1.
U14003 Genomic DNA. Translation: AAA97115.1.
U00096 Genomic DNA. Translation: AAC77176.1.
AP009048 Genomic DNA. Translation: BAE78220.1.

PIR

S56444.

RefSeq

NP_418640.1. NC_000913.2.
YP_492361.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FF3	X-ray	1.90	A/B/C	2-212	[»]
2GT3	NMR	-	A	2-211	[»]
2IEM	NMR	-	A	2-211	[»]

ProteinModelPortal

P0A744.

SMR

P0A744. Positions 2-212.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A744. 3 interactions.

STRING

511145.b4219.

Proteomic databases

PaxDb

P0A744.

PRIDE

P0A744.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77176; AAC77176; b4219.
BAE78220; BAE78220; BAE78220.

GeneID

12930313.
948734.

KEGG

ecj:Y75_p4105.
eco:b4219.

PATRIC

32124013. VBIEscCol129921_4351.

Organism-specific databases

EchoBASE

EB1403.

EcoGene

EG11433. msrA.

Phylogenomic databases

eggNOG

COG0225.

HOGENOM

HOG000263862.

K07304.

OMA

SAIYCTT.

ProtClustDB

PRK00058.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.

Gene expression databases

Genevestigator

P0A744.

Family and domain databases

Gene3D

3.30.1060.10. 1 hit.

HAMAP

MF_01401. MsrA.

InterPro

IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view]

Pfam

PF01625. PMSR. 1 hit.
[Graphical view]

SUPFAM

SSF55068. MsrA. 1 hit.

TIGRFAMs

TIGR00401. msrA. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A744.

Entry information

Entry name

MSRA_ECOLI

Accession

Primary (citable) accession number: P0A744
Secondary accession number(s): P27110, Q2M686

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents