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P0A744 (MSRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide methionine sulfoxide reductase MsrA

Short name=Protein-methionine-S-oxide reductase
EC=1.8.4.11
Alternative name(s):
Peptide-methionine (S)-S-oxide reductase
Short name=Peptide Met(O) reductase
Gene names
Name:msrA
Synonyms:pms
Ordered Locus Names:b4219, JW4178
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. HAMAP-Rule MF_01401

Catalytic activity

Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP-Rule MF_01401

L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP-Rule MF_01401

Sequence similarities

Belongs to the MsrA Met sulfoxide reductase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

lrpP0ACJ01EBI-1129240,EBI-1113316

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 212211Peptide methionine sulfoxide reductase MsrA HAMAP-Rule MF_01401
PRO_0000138546

Sites

Active site521Cysteine sulfenic acid (-SOH) intermediate

Amino acid modifications

Disulfide bond52 ↔ 199Redox-active; alternate Probable
Disulfide bond199 ↔ 207Redox-active; alternate Probable

Experimental info

Mutagenesis521C → S: Loss of activity. Ref.5
Mutagenesis871C → S: No effect. Ref.5
Mutagenesis1991C → S: Decrease in activity. Ref.5
Mutagenesis2071C → S: Decrease in activity. Ref.5

Secondary structure

..................................... 212
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A744 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B9DC86DC1203BD32

FASTA21223,315
        10         20         30         40         50         60 
MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF 

        70         80         90        100        110        120 
WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD 

       130        140        150        160        170        180 
PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF 

       190        200        210 
YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene."
Rahman M.A., Nelson H., Weissbach H., Brot N.
J. Biol. Chem. 267:15549-15551(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-30.
Strain: B.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli."
Boschi-Muller S., Azza S., Sanglier-Cianferani S., Talfournier F., van Dorsselaer A., Branlant G.
J. Biol. Chem. 275:35908-35913(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-52; CYS-87; CYS-199 AND CYS-207.
[6]"Crystal structure of the Escherichia coli peptide methionine sulphoxide reductase at 1.9-A resolution."
Tete-Favier F., Cobessi D., Boschi-Muller S., Azza S., Branlant G., Aubry A.
Structure 8:1167-1178(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M89992 Genomic DNA. Translation: AAA24399.1.
U14003 Genomic DNA. Translation: AAA97115.1.
U00096 Genomic DNA. Translation: AAC77176.1.
AP009048 Genomic DNA. Translation: BAE78220.1.
PIRS56444.
RefSeqNP_418640.1. NC_000913.3.
YP_492361.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FF3X-ray1.90A/B/C2-212[»]
2GT3NMR-A1-212[»]
2IEMNMR-A2-212[»]
ProteinModelPortalP0A744.
SMRP0A744. Positions 2-212.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0A744. 4 interactions.
STRING511145.b4219.

Proteomic databases

PaxDbP0A744.
PRIDEP0A744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77176; AAC77176; b4219.
BAE78220; BAE78220; BAE78220.
GeneID12930313.
948734.
KEGGecj:Y75_p4105.
eco:b4219.
PATRIC32124013. VBIEscCol129921_4351.

Organism-specific databases

EchoBASEEB1403.
EcoGeneEG11433. msrA.

Phylogenomic databases

eggNOGCOG0225.
HOGENOMHOG000263862.
KOK07304.
OMANVEFAIF.
OrthoDBEOG6091JX.
PhylomeDBP0A744.

Enzyme and pathway databases

BioCycEcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.

Gene expression databases

GenevestigatorP0A744.

Family and domain databases

Gene3D3.30.1060.10. 1 hit.
HAMAPMF_01401. MsrA.
InterProIPR028427. Met_Sox_Rdtase.
IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view]
PANTHERPTHR10173. PTHR10173. 1 hit.
PfamPF01625. PMSR. 1 hit.
[Graphical view]
SUPFAMSSF55068. SSF55068. 1 hit.
TIGRFAMsTIGR00401. msrA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A744.
PROP0A744.

Entry information

Entry nameMSRA_ECOLI
AccessionPrimary (citable) accession number: P0A744
Secondary accession number(s): P27110, Q2M686
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene