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P0A744

- MSRA_ECOLI

UniProt

P0A744 - MSRA_ECOLI

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Protein
Peptide methionine sulfoxide reductase MsrA
Gene
msrA, pms, b4219, JW4178
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.UniRule annotation

Catalytic activityi

Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin.UniRule annotation
L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Cysteine sulfenic acid (-SOH) intermediate

GO - Molecular functioni

  1. peptide-methionine (S)-S-oxide reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cellular protein modification process Source: UniProtKB-HAMAP
  2. protein repair Source: InterPro
  3. response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide methionine sulfoxide reductase MsrA (EC:1.8.4.11)
Short name:
Protein-methionine-S-oxide reductase
Alternative name(s):
Peptide-methionine (S)-S-oxide reductase
Short name:
Peptide Met(O) reductase
Gene namesi
Name:msrA
Synonyms:pms
Ordered Locus Names:b4219, JW4178
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11433. msrA.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521C → S: Loss of activity. 1 Publication
Mutagenesisi87 – 871C → S: No effect. 1 Publication
Mutagenesisi199 – 1991C → S: Decrease in activity. 1 Publication
Mutagenesisi207 – 2071C → S: Decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 212211Peptide methionine sulfoxide reductase MsrAUniRule annotation
PRO_0000138546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 199Redox-active; alternate Inferred
Disulfide bondi199 ↔ 207Redox-active; alternate Inferred

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0A744.
PRIDEiP0A744.

Expressioni

Gene expression databases

GenevestigatoriP0A744.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lrpP0ACJ01EBI-1129240,EBI-1113316

Protein-protein interaction databases

IntActiP0A744. 4 interactions.
STRINGi511145.b4219.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 143
Turni30 – 323
Beta strandi35 – 373
Beta strandi44 – 496
Helixi53 – 619
Beta strandi66 – 7712
Helixi83 – 886
Beta strandi89 – 913
Beta strandi94 – 1018
Turni103 – 1053
Helixi108 – 11710
Beta strandi123 – 1275
Beta strandi130 – 1323
Helixi133 – 1353
Beta strandi138 – 1403
Helixi144 – 16320
Beta strandi180 – 1823
Helixi185 – 1873
Helixi190 – 1934

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FF3X-ray1.90A/B/C2-212[»]
2GT3NMR-A1-212[»]
2IEMNMR-A2-212[»]
ProteinModelPortaliP0A744.
SMRiP0A744. Positions 2-212.

Miscellaneous databases

EvolutionaryTraceiP0A744.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0225.
HOGENOMiHOG000263862.
KOiK07304.
OMAiNVEFAIF.
OrthoDBiEOG6091JX.
PhylomeDBiP0A744.

Family and domain databases

Gene3Di3.30.1060.10. 1 hit.
HAMAPiMF_01401. MsrA.
InterProiIPR028427. Met_Sox_Rdtase.
IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view]
PANTHERiPTHR10173. PTHR10173. 1 hit.
PfamiPF01625. PMSR. 1 hit.
[Graphical view]
SUPFAMiSSF55068. SSF55068. 1 hit.
TIGRFAMsiTIGR00401. msrA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A744-1 [UniParc]FASTAAdd to Basket

« Hide

MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM    50
GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV 100
YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA 150
RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG 200
IGGIGVCLPP EA 212
Length:212
Mass (Da):23,315
Last modified:January 23, 2007 - v2
Checksum:iB9DC86DC1203BD32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M89992 Genomic DNA. Translation: AAA24399.1.
U14003 Genomic DNA. Translation: AAA97115.1.
U00096 Genomic DNA. Translation: AAC77176.1.
AP009048 Genomic DNA. Translation: BAE78220.1.
PIRiS56444.
RefSeqiNP_418640.1. NC_000913.3.
YP_492361.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77176; AAC77176; b4219.
BAE78220; BAE78220; BAE78220.
GeneIDi12930313.
948734.
KEGGiecj:Y75_p4105.
eco:b4219.
PATRICi32124013. VBIEscCol129921_4351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M89992 Genomic DNA. Translation: AAA24399.1 .
U14003 Genomic DNA. Translation: AAA97115.1 .
U00096 Genomic DNA. Translation: AAC77176.1 .
AP009048 Genomic DNA. Translation: BAE78220.1 .
PIRi S56444.
RefSeqi NP_418640.1. NC_000913.3.
YP_492361.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FF3 X-ray 1.90 A/B/C 2-212 [» ]
2GT3 NMR - A 1-212 [» ]
2IEM NMR - A 2-212 [» ]
ProteinModelPortali P0A744.
SMRi P0A744. Positions 2-212.
ModBasei Search...

Protein-protein interaction databases

IntActi P0A744. 4 interactions.
STRINGi 511145.b4219.

Proteomic databases

PaxDbi P0A744.
PRIDEi P0A744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77176 ; AAC77176 ; b4219 .
BAE78220 ; BAE78220 ; BAE78220 .
GeneIDi 12930313.
948734.
KEGGi ecj:Y75_p4105.
eco:b4219.
PATRICi 32124013. VBIEscCol129921_4351.

Organism-specific databases

EchoBASEi EB1403.
EcoGenei EG11433. msrA.

Phylogenomic databases

eggNOGi COG0225.
HOGENOMi HOG000263862.
KOi K07304.
OMAi NVEFAIF.
OrthoDBi EOG6091JX.
PhylomeDBi P0A744.

Enzyme and pathway databases

BioCyci EcoCyc:EG11433-MONOMER.
ECOL316407:JW4178-MONOMER.
MetaCyc:EG11433-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A744.
PROi P0A744.

Gene expression databases

Genevestigatori P0A744.

Family and domain databases

Gene3Di 3.30.1060.10. 1 hit.
HAMAPi MF_01401. MsrA.
InterProi IPR028427. Met_Sox_Rdtase.
IPR002569. Met_Sox_Rdtase_MsrA.
[Graphical view ]
PANTHERi PTHR10173. PTHR10173. 1 hit.
Pfami PF01625. PMSR. 1 hit.
[Graphical view ]
SUPFAMi SSF55068. SSF55068. 1 hit.
TIGRFAMsi TIGR00401. msrA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene."
    Rahman M.A., Nelson H., Weissbach H., Brot N.
    J. Biol. Chem. 267:15549-15551(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-30.
    Strain: B.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli."
    Boschi-Muller S., Azza S., Sanglier-Cianferani S., Talfournier F., van Dorsselaer A., Branlant G.
    J. Biol. Chem. 275:35908-35913(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-52; CYS-87; CYS-199 AND CYS-207.
  6. "Crystal structure of the Escherichia coli peptide methionine sulphoxide reductase at 1.9-A resolution."
    Tete-Favier F., Cobessi D., Boschi-Muller S., Azza S., Branlant G., Aubry A.
    Structure 8:1167-1178(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiMSRA_ECOLI
AccessioniPrimary (citable) accession number: P0A744
Secondary accession number(s): P27110, Q2M686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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