ID   MSCL_ECOLI              Reviewed;         136 AA.
AC   P0A742; P23867; Q2M6V5;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   25-JAN-2012, entry version 59.
DE   RecName: Full=Large-conductance mechanosensitive channel;
GN   Name=mscL; Synonyms=yhdC; OrderedLocusNames=b3291, JW3252;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-37.
RX   MEDLINE=94195400; PubMed=7511799; DOI=10.1038/368265a0;
RA   Sukharev S.I., Blount P., Martinac B., Blattner F.R., Kung C.;
RT   "A large-conductance mechanosensitive channel in E. coli encoded by
RT   mscL alone.";
RL   Nature 368:265-268(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94341562; PubMed=8063098; DOI=10.1016/0378-1119(94)90847-8;
RA   Christie G.E., White T.J., Goodwin T.S.;
RT   "A merR homologue at 74 minutes on the Escherichia coli genome.";
RL   Gene 146:131-132(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
RX   MEDLINE=94018648; PubMed=8412700;
RX   DOI=10.1111/j.1365-2958.1993.tb01714.x;
RA   Schloesser A., Hamann A., Bossemeyer D., Schneider E., Bakker E.P.;
RT   "NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and
RT   sequence similarity between TrkA and domains of a family of
RT   dehydrogenases suggest a role for NAD+ in bacterial transport.";
RL   Mol. Microbiol. 9:533-543(1993).
RN   [6]
RP   TOPOLOGY, AND SUBUNIT.
RX   MEDLINE=97045088; PubMed=8890153;
RA   Blount P., Sukharev S.I., Moe P.C., Schroeder M.J., Guy H.R., Kung C.;
RT   "Membrane topology and multimeric structure of a mechanosensitive
RT   channel protein of Escherichia coli.";
RL   EMBO J. 15:4798-4805(1996).
RN   [7]
RP   REVISION OF SUBUNITS.
RX   MEDLINE=99074367; PubMed=9856938; DOI=10.1126/science.282.5397.2220;
RA   Chang G., Spencer R.H., Lee A.T., Barclay M.T., Rees D.C.;
RT   "Structure of the MscL homolog from Mycobacterium tuberculosis: a
RT   gated mechanosensitive ion channel.";
RL   Science 282:2220-2226(1998).
RN   [8]
RP   CHARACTERIZATION, AND PHYSIOLOGICAL ROLE.
RC   STRAIN=K12 / MJF379;
RX   MEDLINE=99219852; PubMed=10202137; DOI=10.1093/emboj/18.7.1730;
RA   Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A.,
RA   Booth I.R.;
RT   "Protection of Escherichia coli cells against extreme turgor by
RT   activation of MscS and MscL mechanosensitive channels: identification
RT   of genes required for MscS activity.";
RL   EMBO J. 18:1730-1737(1999).
RN   [9]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Mechanosensitive channel that opens in response to
CC       stretch forces in the membrane lipid bilayer. Participates in the
CC       regulation of osmotic pressure changes within the cell. Forms a
CC       nonselective ion channel of 2.5 nanosiemens conductance. Opens at
CC       a pressure just below that which would cause cell disruption and
CC       death.
CC   -!- SUBUNIT: Homopentamer.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the MscL family.
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DR   EMBL; U08371; AAA17745.1; -; Unassigned_DNA.
DR   EMBL; L29458; AAA24771.1; -; Genomic_DNA.
DR   EMBL; U18997; AAA58088.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76316.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78001.1; -; Genomic_DNA.
DR   EMBL; X52114; CAA36360.1; -; Genomic_DNA.
DR   PIR; I53826; I53826.
DR   RefSeq; NP_417749.1; NC_000913.2.
DR   PDB; 1KYK; Model; -; A/B/C/D/E=14-96.
DR   PDB; 1KYL; Model; -; A/B/C/D/E=14-96.
DR   PDB; 1KYM; Model; -; A/B/C/D/E=14-96.
DR   PDBsum; 1KYK; -.
DR   PDBsum; 1KYL; -.
DR   PDBsum; 1KYM; -.
DR   ProteinModelPortal; P0A742; -.
DR   SMR; P0A742; 3-133.
DR   DIP; DIP-29827N; -.
DR   IntAct; P0A742; 1.
DR   TCDB; 1.A.22.1.1; large conductance mechanosensitive ion channel (MscL) family.
DR   EnsemblBacteria; EBESCT00000003608; EBESCP00000003608; EBESCG00000002943.
DR   EnsemblBacteria; EBESCT00000014522; EBESCP00000013813; EBESCG00000013583.
DR   GeneID; 947787; -.
DR   GenomeReviews; AP009048_GR; JW3252.
DR   GenomeReviews; U00096_GR; b3291.
DR   KEGG; ecj:JW3252; -.
DR   KEGG; eco:b3291; -.
DR   PATRIC; 32122014; VBIEscCol129921_3384.
DR   EchoBASE; EB1167; -.
DR   EcoGene; EG11180; mscL.
DR   eggNOG; COG1970; -.
DR   GeneTree; EBGT00050000009963; -.
DR   HOGENOM; HBG727604; -.
DR   OMA; VIDFVIV; -.
DR   PhylomeDB; P0A742; -.
DR   ProtClustDB; PRK00567; -.
DR   BioCyc; EcoCyc:EG11180-MONOMER; -.
DR   Genevestigator; P0A742; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanically-gated ion channel activity; IMP:EcoCyc.
DR   GO; GO:0009992; P:cellular water homeostasis; IGI:EcoCyc.
DR   HAMAP; MF_00115; MscL; 1; -.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   KO; K03282; -.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; MS_channel; 1.
DR   TIGRFAMs; TIGR00220; MscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Complete proteome;
KW   Direct protein sequencing; Ion transport; Ionic channel; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    136       Large-conductance mechanosensitive
FT                                channel.
FT                                /FTId=PRO_0000192439.
FT   TOPO_DOM      1     18       Cytoplasmic (Probable).
FT   TRANSMEM     19     38       Helical; (Probable).
FT   TOPO_DOM     39     69       Periplasmic (Probable).
FT   TRANSMEM     70     96       Helical; (Probable).
FT   TOPO_DOM     97    136       Cytoplasmic (Probable).
SQ   SEQUENCE   136 AA;  14957 MW;  18EF7E1BD7DF9491 CRC64;
     MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG GIDFKQFAVT
     LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN KLNRKKEEPA AAPAPTKEEV
     LLTEIRDLLK EQNNRS
//