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Protein

Large-conductance mechanosensitive channel

Gene

mscL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.5 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • mechanically-gated ion channel activity Source: EcoCyc

GO - Biological processi

  • ion transmembrane transport Source: EcoCyc
  • ion transport Source: EcoliWiki

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11180-MONOMER.

Protein family/group databases

TCDBi1.A.22.1.1. the large conductance mechanosensitive ion channel (mscl) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Large-conductance mechanosensitive channel
Gene namesi
Name:mscL
Synonyms:yhdC
Ordered Locus Names:b3291, JW3252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11180. mscL.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicBy similarityAdd BLAST16
Transmembranei17 – 45HelicalBy similarityAdd BLAST29
Topological domaini46 – 74PeriplasmicBy similarityAdd BLAST29
Transmembranei75 – 94HelicalBy similarityAdd BLAST20
Topological domaini95 – 136CytoplasmicBy similarityAdd BLAST42

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49I → F or Y: Decreases the duration of the open state of the channel and decreases mechanosensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001924391 – 136Large-conductance mechanosensitive channelAdd BLAST136

Proteomic databases

PaxDbiP0A742.
PRIDEiP0A742.

Interactioni

Subunit structurei

Homopentamer.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-909797,EBI-909797

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263415. 377 interactors.
DIPiDIP-29827N.
MINTiMINT-8071627.
STRINGi511145.b3291.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KYKmodel-A/B/C/D/E14-96[»]
1KYLmodel-A/B/C/D/E14-96[»]
1KYMmodel-A/B/C/D/E14-96[»]
4LKUX-ray1.45A/B/C/D/E108-136[»]
ProteinModelPortaliP0A742.
SMRiP0A742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The periplasmic loop between the two transmembrane domains modulates channel kinetics, and in particular the length of time the channel remains in the open conformation.1 Publication

Sequence similaritiesi

Belongs to the MscL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M2E. Bacteria.
COG1970. LUCA.
HOGENOMiHOG000226782.
InParanoidiP0A742.
KOiK03282.
PhylomeDBiP0A742.

Family and domain databases

HAMAPiMF_00115. MscL. 1 hit.
InterProiView protein in InterPro
IPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
PANTHERiPTHR30266:SF3. PTHR30266:SF3. 1 hit.
PfamiView protein in Pfam
PF01741. MscL. 1 hit.
PRINTSiPR01264. MECHCHANNEL.
SUPFAMiSSF81330. SSF81330. 1 hit.
TIGRFAMsiTIGR00220. mscL. 1 hit.
PROSITEiView protein in PROSITE
PS01327. MSCL. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG
60 70 80 90 100
GIDFKQFAVT LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN
110 120 130
KLNRKKEEPA AAPAPTKEEV LLTEIRDLLK EQNNRS
Length:136
Mass (Da):14,957
Last modified:June 7, 2005 - v1
Checksum:i18EF7E1BD7DF9491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.
PIRiI53826.
RefSeqiNP_417749.1. NC_000913.3.
WP_000022442.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76316; AAC76316; b3291.
BAE78001; BAE78001; BAE78001.
GeneIDi947787.
KEGGiecj:JW3252.
eco:b3291.
PATRICifig|1411691.4.peg.3441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.
PIRiI53826.
RefSeqiNP_417749.1. NC_000913.3.
WP_000022442.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KYKmodel-A/B/C/D/E14-96[»]
1KYLmodel-A/B/C/D/E14-96[»]
1KYMmodel-A/B/C/D/E14-96[»]
4LKUX-ray1.45A/B/C/D/E108-136[»]
ProteinModelPortaliP0A742.
SMRiP0A742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263415. 377 interactors.
DIPiDIP-29827N.
MINTiMINT-8071627.
STRINGi511145.b3291.

Protein family/group databases

TCDBi1.A.22.1.1. the large conductance mechanosensitive ion channel (mscl) family.

Proteomic databases

PaxDbiP0A742.
PRIDEiP0A742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76316; AAC76316; b3291.
BAE78001; BAE78001; BAE78001.
GeneIDi947787.
KEGGiecj:JW3252.
eco:b3291.
PATRICifig|1411691.4.peg.3441.

Organism-specific databases

EchoBASEiEB1167.
EcoGeneiEG11180. mscL.

Phylogenomic databases

eggNOGiENOG4105M2E. Bacteria.
COG1970. LUCA.
HOGENOMiHOG000226782.
InParanoidiP0A742.
KOiK03282.
PhylomeDBiP0A742.

Enzyme and pathway databases

BioCyciEcoCyc:EG11180-MONOMER.

Miscellaneous databases

PROiPR:P0A742.

Family and domain databases

HAMAPiMF_00115. MscL. 1 hit.
InterProiView protein in InterPro
IPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
PANTHERiPTHR30266:SF3. PTHR30266:SF3. 1 hit.
PfamiView protein in Pfam
PF01741. MscL. 1 hit.
PRINTSiPR01264. MECHCHANNEL.
SUPFAMiSSF81330. SSF81330. 1 hit.
TIGRFAMsiTIGR00220. mscL. 1 hit.
PROSITEiView protein in PROSITE
PS01327. MSCL. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSCL_ECOLI
AccessioniPrimary (citable) accession number: P0A742
Secondary accession number(s): P23867, Q2M6V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: June 7, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.