Reviewed, UniProtKB/Swiss-Prot P0A742 (MSCL_ECOLI)

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Synonyms:	yhdC
Ordered Locus Names:	b3291, JW3252

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Participates in the regulation of osmotic pressure changes within the cell. Forms a nonselective ion channel of 2.5 nanosiemens conductance. Opens at a pressure just below that which would cause cell disruption and death. HAMAP MF_00115
Subunit structure	Homopentamer. Ref.6 Ref.7
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP MF_00115.
Sequence similarities	Belongs to the mscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Ionic channel
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular water homeostasis Ref.8 Inferred from genetic interaction. Source: UniProtKB ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mechanically-gated ion channel activity Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000192439
Regions
	Topological domain	1 – 18	18	Cytoplasmic Probable
	Transmembrane	19 – 38	20	Probable
	Topological domain	39 – 69	31	Periplasmic Probable
	Transmembrane	70 – 96	27	Probable
	Topological domain	97 – 136	40	Cytoplasmic Probable

Sequences

	Sequence		Length	Mass (Da)
	P0A742-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 18EF7E1BD7DF9491	FASTA	136	14,957
	10 20 30 40 50 60 MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG GIDFKQFAVT 70 80 90 100 110 120 LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN KLNRKKEEPA AAPAPTKEEV 130 LLTEIRDLLK EQNNRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large-conductance mechanosensitive channel in E. coli encoded by mscL alone." Sukharev S.I., Blount P., Martinac B., Blattner F.R., Kung C. Nature 368:265-268(1994) [PubMed: 7511799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-37.
[2]	"A merR homologue at 74 minutes on the Escherichia coli genome." Christie G.E., White T.J., Goodwin T.S. Gene 146:131-132(1994) [PubMed: 8063098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Schloesser A., Hamann A., Bossemeyer D., Schneider E., Bakker E.P. Mol. Microbiol. 9:533-543(1993) [PubMed: 8412700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[6]	"Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli." Blount P., Sukharev S.I., Moe P.C., Schroeder M.J., Guy H.R., Kung C. EMBO J. 15:4798-4805(1996) [PubMed: 8890153] [Abstract] Cited for: TOPOLOGY, SUBUNIT.
[7]	"Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel." Chang G., Spencer R.H., Lee A.T., Barclay M.T., Rees D.C. Science 282:2220-2226(1998) [PubMed: 9856938] [Abstract] Cited for: REVISION OF SUBUNITS.
[8]	"Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity." Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R. EMBO J. 18:1730-1737(1999) [PubMed: 10202137] [Abstract] Cited for: CHARACTERIZATION, PHYSIOLOGICAL ROLE. Strain: K12 / MJF379.
[9]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.

PIR

I53826.

RefSeq

AP_004500.1.
NP_417749.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KYK	model	-	A/B/C/D/E	14-96	[»]
1KYL	model	-	A/B/C/D/E	14-96	[»]
1KYM	model	-	A/B/C/D/E	14-96	[»]

SMR

P0A742. Positions 3-133.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29827N.

STRING

P0A742.

Protein family/group databases

TCDB

1.A.22.1.1. large conductance mechanosensitive ion channel (MscL) family.

Genome annotation databases

GeneID

947787.

GenomeReviews

Gene locus JW3252 in contig AP009048_GR.
Gene locus b3291 in contig U00096_GR.

KEGG

ecj:JW3252.
eco:b3291.

Organism-specific databases

EchoBASE

EB1167.

EcoGene

EG11180. mscL.

CMR

Search...

Phylogenomic databases

eggNOG

COG1970.

HOGENOM

HBG727604.

OMA

MIKEFKE.

ProtClustDB

PRK00567.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11180-MONOMER.
ECOL168927:B3291-MONOMER.

Gene expression databases

Genevestigator

P0A742.

Family and domain databases

HAMAP

MF_00115. MscL.
[Tree]

InterPro

IPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
[Graphical view]

Pfam

PF01741. MscL. 1 hit.
[Graphical view]

PRINTS

PR01264. MECHCHANNEL.

SUPFAM

SSF81330. MS_channel. 1 hit.

TIGRFAMs

TIGR00220. mscL. 1 hit.

PROSITE

PS01327. MSCL. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MSCL_ECOLI

Accession

Primary (citable) accession number: P0A742
Secondary accession number(s): P23867, Q2M6V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	March 2, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)