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Protein

Large-conductance mechanosensitive channel

Gene

mscL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Participates in the regulation of osmotic pressure changes within the cell. Forms a nonselective ion channel of 2.5 nanosiemens conductance. Opens at a pressure just below that which would cause cell disruption and death.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • mechanically-gated ion channel activity Source: EcoCyc

GO - Biological processi

  • ion transmembrane transport Source: EcoCyc
  • ion transport Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11180-MONOMER.
ECOL316407:JW3252-MONOMER.

Protein family/group databases

TCDBi1.A.22.1.1. the large conductance mechanosensitive ion channel (mscl) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Large-conductance mechanosensitive channel
Gene namesi
Name:mscL
Synonyms:yhdC
Ordered Locus Names:b3291, JW3252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11180. mscL.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicCuratedAdd
BLAST
Transmembranei19 – 3820HelicalCuratedAdd
BLAST
Topological domaini39 – 6931PeriplasmicCuratedAdd
BLAST
Transmembranei70 – 9627HelicalCuratedAdd
BLAST
Topological domaini97 – 13640CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 136136Large-conductance mechanosensitive channelPRO_0000192439Add
BLAST

Proteomic databases

PaxDbiP0A742.
PRIDEiP0A742.

Interactioni

Subunit structurei

Homopentamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-909797,EBI-909797

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263415. 377 interactions.
DIPiDIP-29827N.
MINTiMINT-8071627.
STRINGi511145.b3291.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYKmodel-A/B/C/D/E14-96[»]
1KYLmodel-A/B/C/D/E14-96[»]
1KYMmodel-A/B/C/D/E14-96[»]
ProteinModelPortaliP0A742.
SMRiP0A742. Positions 3-106, 108-136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MscL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M2E. Bacteria.
COG1970. LUCA.
HOGENOMiHOG000226782.
InParanoidiP0A742.
KOiK03282.
OMAiTEFKEFA.
PhylomeDBiP0A742.

Family and domain databases

HAMAPiMF_00115. MscL. 1 hit.
InterProiIPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
[Graphical view]
PfamiPF01741. MscL. 1 hit.
[Graphical view]
PRINTSiPR01264. MECHCHANNEL.
SUPFAMiSSF81330. SSF81330. 1 hit.
TIGRFAMsiTIGR00220. mscL. 1 hit.
PROSITEiPS01327. MSCL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG
60 70 80 90 100
GIDFKQFAVT LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN
110 120 130
KLNRKKEEPA AAPAPTKEEV LLTEIRDLLK EQNNRS
Length:136
Mass (Da):14,957
Last modified:June 7, 2005 - v1
Checksum:i18EF7E1BD7DF9491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.
PIRiI53826.
RefSeqiNP_417749.1. NC_000913.3.
WP_000022442.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76316; AAC76316; b3291.
BAE78001; BAE78001; BAE78001.
GeneIDi947787.
KEGGiecj:JW3252.
eco:b3291.
PATRICi32122014. VBIEscCol129921_3384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.
PIRiI53826.
RefSeqiNP_417749.1. NC_000913.3.
WP_000022442.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYKmodel-A/B/C/D/E14-96[»]
1KYLmodel-A/B/C/D/E14-96[»]
1KYMmodel-A/B/C/D/E14-96[»]
ProteinModelPortaliP0A742.
SMRiP0A742. Positions 3-106, 108-136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263415. 377 interactions.
DIPiDIP-29827N.
MINTiMINT-8071627.
STRINGi511145.b3291.

Protein family/group databases

TCDBi1.A.22.1.1. the large conductance mechanosensitive ion channel (mscl) family.

Proteomic databases

PaxDbiP0A742.
PRIDEiP0A742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76316; AAC76316; b3291.
BAE78001; BAE78001; BAE78001.
GeneIDi947787.
KEGGiecj:JW3252.
eco:b3291.
PATRICi32122014. VBIEscCol129921_3384.

Organism-specific databases

EchoBASEiEB1167.
EcoGeneiEG11180. mscL.

Phylogenomic databases

eggNOGiENOG4105M2E. Bacteria.
COG1970. LUCA.
HOGENOMiHOG000226782.
InParanoidiP0A742.
KOiK03282.
OMAiTEFKEFA.
PhylomeDBiP0A742.

Enzyme and pathway databases

BioCyciEcoCyc:EG11180-MONOMER.
ECOL316407:JW3252-MONOMER.

Miscellaneous databases

PROiP0A742.

Family and domain databases

HAMAPiMF_00115. MscL. 1 hit.
InterProiIPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
[Graphical view]
PfamiPF01741. MscL. 1 hit.
[Graphical view]
PRINTSiPR01264. MECHCHANNEL.
SUPFAMiSSF81330. SSF81330. 1 hit.
TIGRFAMsiTIGR00220. mscL. 1 hit.
PROSITEiPS01327. MSCL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSCL_ECOLI
AccessioniPrimary (citable) accession number: P0A742
Secondary accession number(s): P23867, Q2M6V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.