Large-conductance mechanosensitive channel - Escherichia coli (strain K12)

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P0A742 (MSCL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Synonyms:	yhdC
Ordered Locus Names:	b3291, JW3252

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Participates in the regulation of osmotic pressure changes within the cell. Forms a nonselective ion channel of 2.5 nanosiemens conductance. Opens at a pressure just below that which would cause cell disruption and death. HAMAP MF_00115
Subunit structure	Homopentamer. Ref.6 Ref.7
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular water homeostasis Inferred from genetic interaction Ref.8. Source: EcoCyc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mechanically-gated ion channel activity Inferred from mutant phenotype Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 136

136

Large-conductance mechanosensitive channel HAMAP MF_00115

PRO_0000192439

Regions

Topological domain

1 – 18

Cytoplasmic Probable

Transmembrane

19 – 38

Helical; Probable

Topological domain

39 – 69

Periplasmic Probable

Transmembrane

70 – 96

Helical; Probable

Topological domain

97 – 136

Cytoplasmic Probable

Sequences

Sequence

Length

Mass (Da)

Tools

P0A742 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 18EF7E1BD7DF9491
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FASTA

136

14,957

        10         20         30         40         50         60 
MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG GIDFKQFAVT 

        70         80         90        100        110        120 
LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN KLNRKKEEPA AAPAPTKEEV 

       130 
LLTEIRDLLK EQNNRS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large-conductance mechanosensitive channel in E. coli encoded by mscL alone." Sukharev S.I., Blount P., Martinac B., Blattner F.R., Kung C. Nature 368:265-268(1994) [PubMed: 7511799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-37.
[2]	"A merR homologue at 74 minutes on the Escherichia coli genome." Christie G.E., White T.J., Goodwin T.S. Gene 146:131-132(1994) [PubMed: 8063098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Schloesser A., Hamann A., Bossemeyer D., Schneider E., Bakker E.P. Mol. Microbiol. 9:533-543(1993) [PubMed: 8412700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[6]	"Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli." Blount P., Sukharev S.I., Moe P.C., Schroeder M.J., Guy H.R., Kung C. EMBO J. 15:4798-4805(1996) [PubMed: 8890153] [Abstract] Cited for: TOPOLOGY, SUBUNIT.
[7]	"Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel." Chang G., Spencer R.H., Lee A.T., Barclay M.T., Rees D.C. Science 282:2220-2226(1998) [PubMed: 9856938] [Abstract] Cited for: REVISION OF SUBUNITS.
[8]	"Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity." Levina N., Toetemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R. EMBO J. 18:1730-1737(1999) [PubMed: 10202137] [Abstract] Cited for: CHARACTERIZATION, PHYSIOLOGICAL ROLE. Strain: K12 / MJF379.
[9]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U08371 Unassigned DNA. Translation: AAA17745.1.
L29458 Genomic DNA. Translation: AAA24771.1.
U18997 Genomic DNA. Translation: AAA58088.1.
U00096 Genomic DNA. Translation: AAC76316.1.
AP009048 Genomic DNA. Translation: BAE78001.1.
X52114 Genomic DNA. Translation: CAA36360.1.

PIR

I53826.

RefSeq

NP_417749.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KYK	model	-	A/B/C/D/E	14-96	[»]
1KYL	model	-	A/B/C/D/E	14-96	[»]
1KYM	model	-	A/B/C/D/E	14-96	[»]

ProteinModelPortal

P0A742.

SMR

P0A742. Positions 3-133.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29827N.

IntAct

P0A742. 1 interaction.

Protein family/group databases

TCDB

1.A.22.1.1. large conductance mechanosensitive ion channel (MscL) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003608; EBESCP00000003608; EBESCG00000002943.
EBESCT00000014522; EBESCP00000013813; EBESCG00000013583.

GeneID

947787.

GenomeReviews

Gene locus JW3252 in contig AP009048_GR.
Gene locus b3291 in contig U00096_GR.

KEGG

ecj:JW3252.
eco:b3291.

PATRIC

32122014. VBIEscCol129921_3384.

Organism-specific databases

EchoBASE

EB1167.

EcoGene

EG11180. mscL.

Phylogenomic databases

eggNOG

COG1970.

GeneTree

EBGT00050000009963.

HOGENOM

HBG727604.

OMA

VIDFVIV.

PhylomeDB

P0A742.

ProtClustDB

PRK00567.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11180-MONOMER.

Gene expression databases

Genevestigator

P0A742.

Family and domain databases

HAMAP

MF_00115. MscL.
[Tree]

InterPro

IPR019823. Mechanosensitive_channel_CS.
IPR001185. MS_channel.
[Graphical view]

K03282.

Pfam

PF01741. MscL. 1 hit.
[Graphical view]

PRINTS

PR01264. MECHCHANNEL.

SUPFAM

SSF81330. MS_channel. 1 hit.

TIGRFAMs

TIGR00220. MscL. 1 hit.

PROSITE

PS01327. MSCL. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MSCL_ECOLI

Accession

Primary (citable) accession number: P0A742
Secondary accession number(s): P23867, Q2M6V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents