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Protein

Cyclic pyranopterin monophosphate synthase accessory protein

Gene

moaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with MoaA, is involved in the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128Sequence analysis1

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11666-MONOMER.
ECOL316407:JW0766-MONOMER.
MetaCyc:EG11666-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthase accessory protein
Alternative name(s):
Molybdenum cofactor biosynthesis protein C
Gene namesi
Name:moaC
Synonyms:chlA3
Ordered Locus Names:b0783, JW0766
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11666. moaC.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51K → A: Reduced activity. 1 Publication1
Mutagenesisi52G → A: Reduced activity. 1 Publication1
Mutagenesisi67K → A: Reduced activity. 1 Publication1
Mutagenesisi76C → A: Reduced activity. 1 Publication1
Mutagenesisi77H → A: Reduced activity. 1 Publication1
Mutagenesisi110G → A: Reduced activity. 1 Publication1
Mutagenesisi112E → A: Reduced activity. 1 Publication1
Mutagenesisi117T → P: Strongly reduced activity. 1 Publication1
Mutagenesisi128D → A: Loss of activity. 1 Publication1
Mutagenesisi131K → A: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000977982 – 161Cyclic pyranopterin monophosphate synthase accessory proteinAdd BLAST160

Proteomic databases

PaxDbiP0A738.
PRIDEiP0A738.

Expressioni

Inductioni

By anaerobiosis, repressed by the molybdenum cofactor.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

BioGridi4262176. 8 interactors.
DIPiDIP-10230N.
IntActiP0A738. 20 interactors.
MINTiMINT-1231800.
STRINGi511145.b0783.

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 21Combined sources3
Beta strandi24 – 35Combined sources12
Helixi38 – 46Combined sources9
Turni47 – 49Combined sources3
Beta strandi50 – 52Combined sources3
Helixi54 – 67Combined sources14
Helixi69 – 72Combined sources4
Beta strandi82 – 91Combined sources10
Helixi92 – 94Combined sources3
Beta strandi96 – 109Combined sources14
Helixi112 – 130Combined sources19
Turni131 – 133Combined sources3
Beta strandi138 – 145Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EKRX-ray2.00A1-161[»]
1EKSX-ray2.50A1-161[»]
4PYAX-ray1.79A3-161[»]
4PYDX-ray3.19A/B/C/D/E/F1-161[»]
ProteinModelPortaliP0A738.
SMRiP0A738.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A738.

Family & Domainsi

Sequence similaritiesi

Belongs to the MoaC family.Curated

Phylogenomic databases

eggNOGiENOG4108YXW. Bacteria.
COG0315. LUCA.
HOGENOMiHOG000228417.
InParanoidiP0A738.
KOiK03637.
OMAiWIPMCHP.
PhylomeDBiP0A738.

Family and domain databases

CDDicd01420. MoaC_PE. 1 hit.
Gene3Di3.30.70.640. 1 hit.
HAMAPiMF_01224_B. MoaC_B. 1 hit.
InterProiIPR023045. Mo_CF_biosynth-C.
IPR023046. Mo_CF_biosynth-C_bac.
IPR002820. Mopterin_CF_biosynth-C_dom.
[Graphical view]
PfamiPF01967. MoaC. 1 hit.
[Graphical view]
SUPFAMiSSF55040. SSF55040. 1 hit.
TIGRFAMsiTIGR00581. moaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLTHINAA GEAHMVDVSA KAETVREARA EAFVTMRSET LAMIIDGRHH
60 70 80 90 100
KGDVFATARI AGIQAAKRTW DLIPLCHPLM LSKVEVNLQA EPEHNRVRIE
110 120 130 140 150
TLCRLTGKTG VEMEALTAAS VAALTIYDMC KAVQKDMVIG PVRLLAKSGG
160
KSGDFKVEAD D
Length:161
Mass (Da):17,467
Last modified:January 23, 2007 - v2
Checksum:i280DAC1EFAAA04AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17D → N in CAA49863 (PubMed:8361352).Curated1
Sequence conflicti92 – 97PEHNRV → RAQSG in CAA49863 (PubMed:8361352).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA. Translation: CAA49863.1.
U00096 Genomic DNA. Translation: AAC73870.1.
AP009048 Genomic DNA. Translation: BAA35441.1.
PIRiG64814.
RefSeqiNP_415304.1. NC_000913.3.
WP_000080885.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73870; AAC73870; b0783.
BAA35441; BAA35441; BAA35441.
GeneIDi945397.
KEGGiecj:JW0766.
eco:b0783.
PATRICi32116767. VBIEscCol129921_0809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA. Translation: CAA49863.1.
U00096 Genomic DNA. Translation: AAC73870.1.
AP009048 Genomic DNA. Translation: BAA35441.1.
PIRiG64814.
RefSeqiNP_415304.1. NC_000913.3.
WP_000080885.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EKRX-ray2.00A1-161[»]
1EKSX-ray2.50A1-161[»]
4PYAX-ray1.79A3-161[»]
4PYDX-ray3.19A/B/C/D/E/F1-161[»]
ProteinModelPortaliP0A738.
SMRiP0A738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262176. 8 interactors.
DIPiDIP-10230N.
IntActiP0A738. 20 interactors.
MINTiMINT-1231800.
STRINGi511145.b0783.

Proteomic databases

PaxDbiP0A738.
PRIDEiP0A738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73870; AAC73870; b0783.
BAA35441; BAA35441; BAA35441.
GeneIDi945397.
KEGGiecj:JW0766.
eco:b0783.
PATRICi32116767. VBIEscCol129921_0809.

Organism-specific databases

EchoBASEiEB1617.
EcoGeneiEG11666. moaC.

Phylogenomic databases

eggNOGiENOG4108YXW. Bacteria.
COG0315. LUCA.
HOGENOMiHOG000228417.
InParanoidiP0A738.
KOiK03637.
OMAiWIPMCHP.
PhylomeDBiP0A738.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciEcoCyc:EG11666-MONOMER.
ECOL316407:JW0766-MONOMER.
MetaCyc:EG11666-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A738.
PROiP0A738.

Family and domain databases

CDDicd01420. MoaC_PE. 1 hit.
Gene3Di3.30.70.640. 1 hit.
HAMAPiMF_01224_B. MoaC_B. 1 hit.
InterProiIPR023045. Mo_CF_biosynth-C.
IPR023046. Mo_CF_biosynth-C_bac.
IPR002820. Mopterin_CF_biosynth-C_dom.
[Graphical view]
PfamiPF01967. MoaC. 1 hit.
[Graphical view]
SUPFAMiSSF55040. SSF55040. 1 hit.
TIGRFAMsiTIGR00581. moaC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOAC_ECOLI
AccessioniPrimary (citable) accession number: P0A738
Secondary accession number(s): P30747, P77530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.