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Protein

Cell division topological specificity factor

Gene

minE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • barrier septum site selection Source: EcoCyc
  • regulation of barrier septum assembly Source: EcoliWiki
  • regulation of cell division Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG10598-MONOMER.
ECOL316407:JW1163-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division topological specificity factor
Gene namesi
Name:minE
Ordered Locus Names:b1174, JW1163
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10598. minE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

In a minCDE operon disruption (minC-minD-minE), cells divide not only at midpoint but also at their poles, yielding small minicells and long rods. Loss of polar localization of several polar-localized proteins including GroEL-GroES, TnaA and YqjD.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8888Cell division topological specificity factorPRO_0000205874Add
BLAST

Proteomic databases

EPDiP0A734.
PaxDbiP0A734.
PRIDEiP0A734.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1118020,EBI-1118020
minDP0AEZ34EBI-1118020,EBI-554545

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262867. 340 interactions.
DIPiDIP-35912N.
IntActiP0A734. 19 interactions.
STRINGi511145.b1174.

Structurei

Secondary structure

1
88
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2512Combined sources
Helixi32 – 343Combined sources
Helixi39 – 5315Combined sources
Helixi58 – 603Combined sources
Beta strandi61 – 688Combined sources
Turni69 – 713Combined sources
Beta strandi72 – 8110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EV0NMR-A/B31-88[»]
3R9IX-ray2.60E/F/G/H12-31[»]
3R9JX-ray4.30C/D12-88[»]
ProteinModelPortaliP0A734.
SMRiP0A734. Positions 31-88.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A734.

Family & Domainsi

Sequence similaritiesi

Belongs to the MinE family.Curated

Phylogenomic databases

eggNOGiENOG4105VJP. Bacteria.
COG0851. LUCA.
HOGENOMiHOG000218362.
InParanoidiP0A734.
KOiK03608.
OMAiQIVIAHE.
PhylomeDBiP0A734.

Family and domain databases

Gene3Di3.30.1070.10. 1 hit.
HAMAPiMF_00262. MinE. 1 hit.
InterProiIPR005527. MinE.
[Graphical view]
PfamiPF03776. MinE. 1 hit.
[Graphical view]
SUPFAMiSSF55229. SSF55229. 1 hit.
TIGRFAMsiTIGR01215. minE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLDFFLSR KKNTANIAKE RLQIIVAERR RSDAEPHYLP QLRKDILEVI
60 70 80
CKYVQIDPEM VTVQLEQKDG DISILELNVT LPEAEELK
Length:88
Mass (Da):10,235
Last modified:June 7, 2005 - v1
Checksum:i04DDFB4EA24F96C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03153 Genomic DNA. Translation: AAB59063.1.
U00096 Genomic DNA. Translation: AAC74258.1.
AP009048 Genomic DNA. Translation: BAA36008.1.
PIRiC31877. CEECTF.
RefSeqiNP_415692.1. NC_000913.3.
WP_001185665.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74258; AAC74258; b1174.
BAA36008; BAA36008; BAA36008.
GeneIDi945740.
KEGGiecj:JW1163.
eco:b1174.
PATRICi32117592. VBIEscCol129921_1218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03153 Genomic DNA. Translation: AAB59063.1.
U00096 Genomic DNA. Translation: AAC74258.1.
AP009048 Genomic DNA. Translation: BAA36008.1.
PIRiC31877. CEECTF.
RefSeqiNP_415692.1. NC_000913.3.
WP_001185665.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EV0NMR-A/B31-88[»]
3R9IX-ray2.60E/F/G/H12-31[»]
3R9JX-ray4.30C/D12-88[»]
ProteinModelPortaliP0A734.
SMRiP0A734. Positions 31-88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262867. 340 interactions.
DIPiDIP-35912N.
IntActiP0A734. 19 interactions.
STRINGi511145.b1174.

Proteomic databases

EPDiP0A734.
PaxDbiP0A734.
PRIDEiP0A734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74258; AAC74258; b1174.
BAA36008; BAA36008; BAA36008.
GeneIDi945740.
KEGGiecj:JW1163.
eco:b1174.
PATRICi32117592. VBIEscCol129921_1218.

Organism-specific databases

EchoBASEiEB0593.
EcoGeneiEG10598. minE.

Phylogenomic databases

eggNOGiENOG4105VJP. Bacteria.
COG0851. LUCA.
HOGENOMiHOG000218362.
InParanoidiP0A734.
KOiK03608.
OMAiQIVIAHE.
PhylomeDBiP0A734.

Enzyme and pathway databases

BioCyciEcoCyc:EG10598-MONOMER.
ECOL316407:JW1163-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A734.
PROiP0A734.

Family and domain databases

Gene3Di3.30.1070.10. 1 hit.
HAMAPiMF_00262. MinE. 1 hit.
InterProiIPR005527. MinE.
[Graphical view]
PfamiPF03776. MinE. 1 hit.
[Graphical view]
SUPFAMiSSF55229. SSF55229. 1 hit.
TIGRFAMsiTIGR01215. minE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMINE_ECOLI
AccessioniPrimary (citable) accession number: P0A734
Secondary accession number(s): P18198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.