Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylglyoxal synthase

Gene

mgsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Glycerone phosphate = methylglyoxal + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei711

GO - Molecular functioni

  • methylglyoxal synthase activity Source: EcoCyc

GO - Biological processi

  • methylglyoxal biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

BioCyciEcoCyc:METHGLYSYN-MONOMER.
ECOL316407:JW5129-MONOMER.
MetaCyc:METHGLYSYN-MONOMER.
BRENDAi4.2.3.3. 2026.
SABIO-RKP0A731.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylglyoxal synthase (EC:4.2.3.3)
Short name:
MGS
Gene namesi
Name:mgsA
Synonyms:yccG
Ordered Locus Names:b0963, JW5129
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12307. mgsA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001786251 – 152Methylglyoxal synthaseAdd BLAST152

Proteomic databases

PaxDbiP0A731.
PRIDEiP0A731.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

BioGridi4260938. 3 interactors.
849948. 1 interactor.
DIPiDIP-48252N.
STRINGi511145.b0963.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi13 – 18Combined sources6
Helixi20 – 22Combined sources3
Helixi23 – 32Combined sources10
Helixi34 – 37Combined sources4
Beta strandi40 – 45Combined sources6
Helixi48 – 56Combined sources9
Beta strandi61 – 63Combined sources3
Helixi66 – 68Combined sources3
Helixi70 – 79Combined sources10
Beta strandi85 – 89Combined sources5
Helixi99 – 111Combined sources13
Beta strandi116 – 119Combined sources4
Helixi120 – 127Combined sources8
Helixi130 – 133Combined sources4
Beta strandi136 – 141Combined sources6
Helixi143 – 147Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B93X-ray1.90A/B/C1-152[»]
1EGHX-ray2.00A/B/C/D/E/F1-152[»]
1IK4X-ray2.00A/B/C/D/E/F1-152[»]
1S89X-ray2.22A/B/C/D/E/F1-152[»]
1S8AX-ray2.20A/B/C/D/E/F1-152[»]
ProteinModelPortaliP0A731.
SMRiP0A731.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A731.

Family & Domainsi

Sequence similaritiesi

Belongs to the methylglyoxal synthase family.Curated

Phylogenomic databases

eggNOGiENOG4108Z5K. Bacteria.
COG1803. LUCA.
HOGENOMiHOG000283729.
InParanoidiP0A731.
KOiK01734.
OMAiMTPQPHE.
PhylomeDBiP0A731.

Family and domain databases

CDDicd01422. MGS. 1 hit.
Gene3Di3.40.50.1380. 1 hit.
HAMAPiMF_00549. Methylglyoxal_synth. 1 hit.
InterProiIPR004363. Methylgl_synth.
IPR018148. Methylglyoxal_synth_AS.
IPR011607. MGS-like_dom.
[Graphical view]
PfamiPF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF006614. Methylglyox_syn. 1 hit.
SMARTiSM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
TIGRFAMsiTIGR00160. MGSA. 1 hit.
PROSITEiPS01335. METHYLGLYOXAL_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELTTRTLPA RKHIALVAHD HCKQMLMSWV ERHQPLLEQH VLYATGTTGN
60 70 80 90 100
LISRATGMNV NAMLSGPMGG DQQVGALISE GKIDVLIFFW DPLNAVPHDP
110 120 130 140 150
DVKALLRLAT VWNIPVATNV ATADFIIQSP HFNDAVDILI PDYQRYLADR

LK
Length:152
Mass (Da):16,919
Last modified:June 7, 2005 - v1
Checksum:i85157193BD4CE72D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 2ME → WK in J04726 (PubMed:2542273).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04726 Genomic DNA. No translation available.
Y11249 Genomic DNA. Translation: CAA72119.1.
U00096 Genomic DNA. Translation: AAC74049.2.
AP009048 Genomic DNA. Translation: BAA35728.2.
PIRiB64837.
RefSeqiNP_415483.2. NC_000913.3.
WP_000424181.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74049; AAC74049; b0963.
BAA35728; BAA35728; BAA35728.
GeneIDi945574.
KEGGiecj:JW5129.
eco:b0963.
PATRICi32117145. VBIEscCol129921_0997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04726 Genomic DNA. No translation available.
Y11249 Genomic DNA. Translation: CAA72119.1.
U00096 Genomic DNA. Translation: AAC74049.2.
AP009048 Genomic DNA. Translation: BAA35728.2.
PIRiB64837.
RefSeqiNP_415483.2. NC_000913.3.
WP_000424181.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B93X-ray1.90A/B/C1-152[»]
1EGHX-ray2.00A/B/C/D/E/F1-152[»]
1IK4X-ray2.00A/B/C/D/E/F1-152[»]
1S89X-ray2.22A/B/C/D/E/F1-152[»]
1S8AX-ray2.20A/B/C/D/E/F1-152[»]
ProteinModelPortaliP0A731.
SMRiP0A731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260938. 3 interactors.
849948. 1 interactor.
DIPiDIP-48252N.
STRINGi511145.b0963.

Proteomic databases

PaxDbiP0A731.
PRIDEiP0A731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74049; AAC74049; b0963.
BAA35728; BAA35728; BAA35728.
GeneIDi945574.
KEGGiecj:JW5129.
eco:b0963.
PATRICi32117145. VBIEscCol129921_0997.

Organism-specific databases

EchoBASEiEB2213.
EcoGeneiEG12307. mgsA.

Phylogenomic databases

eggNOGiENOG4108Z5K. Bacteria.
COG1803. LUCA.
HOGENOMiHOG000283729.
InParanoidiP0A731.
KOiK01734.
OMAiMTPQPHE.
PhylomeDBiP0A731.

Enzyme and pathway databases

BioCyciEcoCyc:METHGLYSYN-MONOMER.
ECOL316407:JW5129-MONOMER.
MetaCyc:METHGLYSYN-MONOMER.
BRENDAi4.2.3.3. 2026.
SABIO-RKP0A731.

Miscellaneous databases

EvolutionaryTraceiP0A731.
PROiP0A731.

Family and domain databases

CDDicd01422. MGS. 1 hit.
Gene3Di3.40.50.1380. 1 hit.
HAMAPiMF_00549. Methylglyoxal_synth. 1 hit.
InterProiIPR004363. Methylgl_synth.
IPR018148. Methylglyoxal_synth_AS.
IPR011607. MGS-like_dom.
[Graphical view]
PfamiPF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF006614. Methylglyox_syn. 1 hit.
SMARTiSM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
TIGRFAMsiTIGR00160. MGSA. 1 hit.
PROSITEiPS01335. METHYLGLYOXAL_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGSA_ECOLI
AccessioniPrimary (citable) accession number: P0A731
Secondary accession number(s): P37066
, P75872, Q8XD91, Q9R7Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.