Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylglyoxal synthase

Gene

mgsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.UniRule annotation3 Publications

Catalytic activityi

Glycerone phosphate = methylglyoxal + phosphate.UniRule annotation3 Publications

Enzyme regulationi

Inhibited by inorganic phosphate ions (PubMed:9665712, PubMed:15049687). Competitively inhibited by phosphoglycolate (PubMed:15049687). Competitively inhibited by phosphoglycolohydroxamate (PubMed:11389594).3 Publications

Kineticsi

kcat is 220 sec(-1) with dihydroxyacetone phosphate as substrate.1 Publication
  1. KM=0.2 mM for dihydroxyacetone phosphate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei19SubstrateUniRule annotationCombined sources1 Publication1
    Binding sitei23SubstrateUniRule annotationCombined sources3 Publications1
    Active sitei71Proton donor/acceptorUniRule annotation4 Publications1
    Binding sitei98SubstrateUniRule annotationCombined sources2 Publications1

    GO - Molecular functioni

    • methylglyoxal synthase activity Source: EcoCyc

    GO - Biological processi

    • methylglyoxal biosynthetic process Source: EcoCyc

    Keywordsi

    Molecular functionLyase

    Enzyme and pathway databases

    BioCyciEcoCyc:METHGLYSYN-MONOMER
    MetaCyc:METHGLYSYN-MONOMER
    BRENDAi4.2.3.3 2026
    SABIO-RKiP0A731

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylglyoxal synthaseUniRule annotation (EC:4.2.3.3UniRule annotation3 Publications)
    Short name:
    MGSUniRule annotation
    Gene namesi
    Name:mgsAUniRule annotation
    Synonyms:yccG
    Ordered Locus Names:b0963, JW5129
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12307 mgsA

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi20D → E: Strongly decreased catalytic efficiency. 1 Publication1
    Mutagenesisi20D → N: Causes inhibition by substrate leading to loss of enzyme activity. 1 Publication1
    Mutagenesisi71D → E or N: Strongly decreased catalytic efficiency. 1 Publication1
    Mutagenesisi91D → E: Strongly decreased catalytic efficiency. 1 Publication1
    Mutagenesisi91D → N: Causes inhibition by substrate leading to loss of enzyme activity. 1 Publication1
    Mutagenesisi98H → N or Q: Strongly decreased catalytic efficiency. 1 Publication1
    Mutagenesisi101D → E or N: Strongly decreased catalytic efficiency. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001786251 – 152Methylglyoxal synthaseAdd BLAST152

    Proteomic databases

    PaxDbiP0A731
    PRIDEiP0A731

    Interactioni

    Subunit structurei

    Homohexamer.4 Publications

    Protein-protein interaction databases

    BioGridi4260938, 11 interactors
    849948, 1 interactor
    DIPiDIP-48252N
    STRINGi316385.ECDH10B_1033

    Structurei

    Secondary structure

    1152
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 8Combined sources6
    Beta strandi13 – 18Combined sources6
    Helixi20 – 22Combined sources3
    Helixi23 – 32Combined sources10
    Helixi34 – 37Combined sources4
    Beta strandi40 – 45Combined sources6
    Helixi48 – 56Combined sources9
    Beta strandi61 – 63Combined sources3
    Helixi66 – 68Combined sources3
    Helixi70 – 79Combined sources10
    Beta strandi85 – 89Combined sources5
    Helixi99 – 111Combined sources13
    Beta strandi116 – 119Combined sources4
    Helixi120 – 127Combined sources8
    Helixi130 – 133Combined sources4
    Beta strandi136 – 141Combined sources6
    Helixi143 – 147Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1B93X-ray1.90A/B/C1-152[»]
    1EGHX-ray2.00A/B/C/D/E/F1-152[»]
    1IK4X-ray2.00A/B/C/D/E/F1-152[»]
    1S89X-ray2.22A/B/C/D/E/F1-152[»]
    1S8AX-ray2.20A/B/C/D/E/F1-152[»]
    ProteinModelPortaliP0A731
    SMRiP0A731
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A731

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 152MGS-likeUniRule annotationAdd BLAST147

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni45 – 48Substrate bindingUniRule annotationCombined sources3 Publications4
    Regioni65 – 66Substrate bindingUniRule annotationCombined sources3 Publications2

    Sequence similaritiesi

    Belongs to the methylglyoxal synthase family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4108Z5K Bacteria
    COG1803 LUCA
    HOGENOMiHOG000283729
    InParanoidiP0A731
    KOiK01734
    OMAiRACDVHN
    PhylomeDBiP0A731

    Family and domain databases

    CDDicd01422 MGS, 1 hit
    Gene3Di3.40.50.1380, 1 hit
    HAMAPiMF_00549 Methylglyoxal_synth, 1 hit
    InterProiView protein in InterPro
    IPR004363 Methylgl_synth
    IPR018148 Methylglyoxal_synth_AS
    IPR011607 MGS-like_dom
    IPR036914 MGS-like_dom_sf
    PANTHERiPTHR30492 PTHR30492, 1 hit
    PfamiView protein in Pfam
    PF02142 MGS, 1 hit
    PIRSFiPIRSF006614 Methylglyox_syn, 1 hit
    SMARTiView protein in SMART
    SM00851 MGS, 1 hit
    SUPFAMiSSF52335 SSF52335, 1 hit
    TIGRFAMsiTIGR00160 MGSA, 1 hit
    PROSITEiView protein in PROSITE
    PS01335 METHYLGLYOXAL_SYNTH, 1 hit
    PS51855 MGS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A731-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MELTTRTLPA RKHIALVAHD HCKQMLMSWV ERHQPLLEQH VLYATGTTGN
    60 70 80 90 100
    LISRATGMNV NAMLSGPMGG DQQVGALISE GKIDVLIFFW DPLNAVPHDP
    110 120 130 140 150
    DVKALLRLAT VWNIPVATNV ATADFIIQSP HFNDAVDILI PDYQRYLADR

    LK
    Length:152
    Mass (Da):16,919
    Last modified:June 7, 2005 - v1
    Checksum:i85157193BD4CE72D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti1 – 2ME → WK in J04726 (PubMed:2542273).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J04726 Genomic DNA No translation available.
    Y11249 Genomic DNA Translation: CAA72119.1
    U00096 Genomic DNA Translation: AAC74049.2
    AP009048 Genomic DNA Translation: BAA35728.2
    PIRiB64837
    RefSeqiNP_415483.2, NC_000913.3
    WP_000424181.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74049; AAC74049; b0963
    BAA35728; BAA35728; BAA35728
    GeneIDi945574
    KEGGiecj:JW5129
    eco:b0963
    PATRICifig|1411691.4.peg.1311

    Similar proteinsi

    Entry informationi

    Entry nameiMGSA_ECOLI
    AccessioniPrimary (citable) accession number: P0A731
    Secondary accession number(s): P37066
    , P75872, Q8XD91, Q9R7Q3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: March 28, 2018
    This is version 99 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health