Reviewed, UniProtKB/Swiss-Prot P0A725 (LPXC_ECOLI)

Names and origin

Protein names

Recommended name:
    UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase
    EC=3.5.1.-
Alternative name(s):
    UDP-3-O-acyl-GlcNAc deacetylase
    Protein envA

Gene names

Name:	lpxC
Synonyms:	asmB, envA
Ordered Locus Names:	b0096, JW0094

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Ref.6
Catalytic activity	UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine + H2O = UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine + acetate. HAMAP MF_00388
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 2/6. HAMAP MF_00388
Sequence similarities	Belongs to the lpxC family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase HAMAP MF_00388		PRO_0000191929
Natural variations
	Natural variant	50	1	F → S in ASMB2/3; reduced activity.
	Natural variant	210	1	G → S in ASMB1; reduced activity.

Sequences

	Sequence		Length	Mass (Da)
	P0A725-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: CA439A00813463AB	FASTA	305	33,956
	10 20 30 40 50 60 MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT 70 80 90 100 110 120 MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI 130 140 150 160 170 180 DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD 190 200 210 220 230 240 AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM 250 260 270 280 290 300 LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP SAVLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli." Beall B., Lutkenhaus J. J. Bacteriol. 169:5408-5415(1987) [PubMed: 2824434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The nucleotide sequence of the essential cell-division gene ftsZ of Escherichia coli." Yi Q.-M., Lutkenhaus J. Gene 36:241-247(1985) [PubMed: 3000876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82. Strain: K12.
[6]	"The second step of lipid A biosynthesis, UDP-3-O-acyl-GlcNAc deacetylase is encoded by the pleotropic permeability/cell division gene envA of E.coli." Young K., Silver L.L., Bramhill D., Caceres C.A., Stachula S.A., Shelly S.E., Raetz C.R.H., Anderson M.S. FASEB J. 7:1268-1268(1993) Cited for: FUNCTION.
[7]	"asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC)." Kloser A.W., Laird M.W., Misra R. J. Bacteriol. 178:5138-5143(1996) [PubMed: 8752330] [Abstract] Cited for: VARIANTS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19211 Genomic DNA. Translation: AAA83849.1. X55034 Genomic DNA. Translation: CAA38873.1. U00096 Genomic DNA. Translation: AAC73207.1. AP009048 Genomic DNA. Translation: BAB96664.1.
PIR	BVECEA. A28381.
RefSeq	AP_000759.1. NP_414638.1.
3D structure databases
HSSP	HSSP built from PDB template 1P42 based on UniProtKB O67648.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A725.
Genome annotation databases
GeneID	944816.
GenomeReviews	Gene locus JW0094 in contig AP009048_GR. Gene locus b0096 in contig U00096_GR.
KEGG	ecj:JW0094. eco:b0096.
Organism-specific databases
EchoBASE	EB0261.
EcoGene	EG10265. lpxC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A725.
OMA	TGVGVHS.
Enzyme and pathway databases
BioCyc	EcoCyc:UDPACYLGLCNACDEACETYL-MON. MetaCyc:UDPACYLGLCNACDEACETYL-MON.
Gene expression databases
Genevestigator	P0A725.
Family and domain databases
HAMAP	MF_00388. [Tree]
InterPro	IPR004463. UDP-acyl_GlcNac_deAcase. IPR011334. UDP-acyl_GlcNac_deAcase_C. IPR015870. UDP-acyl_N-AcGlcN_deAcase_N. [Graphical view]
Gene3D	G3DSA:3.30.1700.10. UDP-acyl_N-AcGlcN_deAcase_C. 1 hit. G3DSA:3.30.230.20. UDP-acyl_N-AcGlcN_deAcase_N. 1 hit.
Pfam	PF03331. LpxC. 1 hit. [Graphical view]
ProDom	PD011499. Lipid_A_LpxC. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00325. lpxC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXC_ECOLI

Accession

Primary (citable) accession number: P0A725
Secondary accession number(s): P07652

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)