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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125Substrate1
Binding sitei144Substrate1
Binding sitei161Substrate1

GO - Molecular functioni

  • acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: EcoliWiki
  • identical protein binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
ECOL316407:JW0176-MONOMER.
MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
BRENDAi2.3.1.129. 2026.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:b0181, JW0176
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10545. lpxA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001880461 – 262Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST262

Proteomic databases

EPDiP0A722.
PaxDbiP0A722.
PRIDEiP0A722.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation3 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261091. 384 interactors.
DIPiDIP-48043N.
IntActiP0A722. 36 interactors.
MINTiMINT-1234984.
STRINGi511145.b0181.

Structurei

Secondary structure

1262
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi12 – 14Combined sources3
Beta strandi36 – 38Combined sources3
Beta strandi52 – 56Combined sources5
Beta strandi67 – 70Combined sources4
Beta strandi83 – 86Combined sources4
Beta strandi97 – 99Combined sources3
Turni103 – 106Combined sources4
Beta strandi107 – 111Combined sources5
Beta strandi179 – 181Combined sources3
Beta strandi185 – 188Combined sources4
Turni189 – 192Combined sources4
Beta strandi193 – 197Combined sources5
Helixi199 – 204Combined sources6
Helixi209 – 223Combined sources15
Helixi229 – 240Combined sources12
Helixi244 – 246Combined sources3
Helixi247 – 255Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LXAX-ray2.60A1-262[»]
2AQ9X-ray1.80A1-262[»]
2JF2X-ray1.80A1-262[»]
2JF3X-ray3.00A1-262[»]
2QIAX-ray1.74A1-262[»]
2QIVX-ray1.85X1-262[»]
4J09X-ray1.90A1-262[»]
ProteinModelPortaliP0A722.
SMRiP0A722.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A722.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 76Substrate binding4

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
HOGENOMiHOG000294326.
InParanoidiP0A722.
KOiK00677.
OMAiGDDGLFM.
PhylomeDBiP0A722.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV
60 70 80 90 100
NGHTKIGRDN EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR
110 120 130 140 150
GTVQGGGLTK VGSDNLLMIN AHIAHDCTVG NRCILANNAT LAGHVSVDDF
160 170 180 190 200
AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD VPPYVIAQGN HATPFGVNIE
210 220 230 240 250
GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA ETYPEVKAFT
260
DFFARSTRGL IR
Length:262
Mass (Da):28,080
Last modified:June 7, 2005 - v1
Checksum:iB42B076F0045B44C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64 – 65QF → SV in AAC36918 (PubMed:3277952).Curated2
Sequence conflicti125H → D in AAC36918 (PubMed:3277952).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti189G → S in strain: SM101; temperature-sensitive. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36918.1.
U70214 Genomic DNA. Translation: AAB08610.1.
U00096 Genomic DNA. Translation: AAC73292.1.
AP009048 Genomic DNA. Translation: BAA77856.2.
PIRiE64742. XUECDP.
RefSeqiNP_414723.1. NC_000913.3.
WP_000565966.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73292; AAC73292; b0181.
BAA77856; BAA77856; BAA77856.
GeneIDi944849.
KEGGiecj:JW0176.
eco:b0181.
PATRICi32115473. VBIEscCol129921_0188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36918.1.
U70214 Genomic DNA. Translation: AAB08610.1.
U00096 Genomic DNA. Translation: AAC73292.1.
AP009048 Genomic DNA. Translation: BAA77856.2.
PIRiE64742. XUECDP.
RefSeqiNP_414723.1. NC_000913.3.
WP_000565966.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LXAX-ray2.60A1-262[»]
2AQ9X-ray1.80A1-262[»]
2JF2X-ray1.80A1-262[»]
2JF3X-ray3.00A1-262[»]
2QIAX-ray1.74A1-262[»]
2QIVX-ray1.85X1-262[»]
4J09X-ray1.90A1-262[»]
ProteinModelPortaliP0A722.
SMRiP0A722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261091. 384 interactors.
DIPiDIP-48043N.
IntActiP0A722. 36 interactors.
MINTiMINT-1234984.
STRINGi511145.b0181.

Proteomic databases

EPDiP0A722.
PaxDbiP0A722.
PRIDEiP0A722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73292; AAC73292; b0181.
BAA77856; BAA77856; BAA77856.
GeneIDi944849.
KEGGiecj:JW0176.
eco:b0181.
PATRICi32115473. VBIEscCol129921_0188.

Organism-specific databases

EchoBASEiEB0540.
EcoGeneiEG10545. lpxA.

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
HOGENOMiHOG000294326.
InParanoidiP0A722.
KOiK00677.
OMAiGDDGLFM.
PhylomeDBiP0A722.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.
BioCyciEcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
ECOL316407:JW0176-MONOMER.
MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
BRENDAi2.3.1.129. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A722.
PROiP0A722.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPXA_ECOLI
AccessioniPrimary (citable) accession number: P0A722
Secondary accession number(s): P10440, P78243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.