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P0A722

- LPXA_ECOLI

UniProt

P0A722 - LPXA_ECOLI

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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Substrate
Binding sitei144 – 1441Substrate
Binding sitei161 – 1611Substrate

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: EcoliWiki
  2. identical protein binding Source: EcoCyc

GO - Biological processi

  1. lipid A biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
ECOL316407:JW0176-MONOMER.
MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:b0181, JW0176
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10545. lpxA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferasePRO_0000188046Add
BLAST

Proteomic databases

PaxDbiP0A722.
PRIDEiP0A722.

Expressioni

Gene expression databases

GenevestigatoriP0A722.

Interactioni

Subunit structurei

Homotrimer.3 PublicationsUniRule annotation

Protein-protein interaction databases

DIPiDIP-48043N.
IntActiP0A722. 36 interactions.
MINTiMINT-1234984.
STRINGi511145.b0181.

Structurei

Secondary structure

1
262
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83
Beta strandi12 – 143
Beta strandi36 – 383
Beta strandi52 – 565
Beta strandi67 – 704
Beta strandi83 – 864
Beta strandi97 – 993
Turni103 – 1064
Beta strandi107 – 1115
Beta strandi179 – 1813
Beta strandi185 – 1884
Turni189 – 1924
Beta strandi193 – 1975
Helixi199 – 2046
Helixi209 – 22315
Helixi229 – 24012
Helixi244 – 2463
Helixi247 – 2559

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LXAX-ray2.60A1-262[»]
2AQ9X-ray1.80A1-262[»]
2JF2X-ray1.80A1-262[»]
2JF3X-ray3.00A1-262[»]
2QIAX-ray1.74A1-262[»]
2QIVX-ray1.85X1-262[»]
4J09X-ray1.90A1-262[»]
ProteinModelPortaliP0A722.
SMRiP0A722. Positions 1-262.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A722.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni73 – 764Substrate binding

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1043.
HOGENOMiHOG000294326.
InParanoidiP0A722.
KOiK00677.
OMAiDCQDKKY.
OrthoDBiEOG6F81P1.
PhylomeDBiP0A722.

Family and domain databases

Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A722-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV
60 70 80 90 100
NGHTKIGRDN EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR
110 120 130 140 150
GTVQGGGLTK VGSDNLLMIN AHIAHDCTVG NRCILANNAT LAGHVSVDDF
160 170 180 190 200
AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD VPPYVIAQGN HATPFGVNIE
210 220 230 240 250
GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA ETYPEVKAFT
260
DFFARSTRGL IR
Length:262
Mass (Da):28,080
Last modified:June 7, 2005 - v1
Checksum:iB42B076F0045B44C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 652QF → SV in AAC36918. (PubMed:3277952)Curated
Sequence conflicti125 – 1251H → D in AAC36918. (PubMed:3277952)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti189 – 1891G → S in strain: SM101; temperature-sensitive.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19334 Genomic DNA. Translation: AAC36918.1.
U70214 Genomic DNA. Translation: AAB08610.1.
U00096 Genomic DNA. Translation: AAC73292.1.
AP009048 Genomic DNA. Translation: BAA77856.2.
PIRiE64742. XUECDP.
RefSeqiNP_414723.1. NC_000913.3.
YP_488483.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73292; AAC73292; b0181.
BAA77856; BAA77856; BAA77856.
GeneIDi12932097.
944849.
KEGGiecj:Y75_p0177.
eco:b0181.
PATRICi32115473. VBIEscCol129921_0188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19334 Genomic DNA. Translation: AAC36918.1 .
U70214 Genomic DNA. Translation: AAB08610.1 .
U00096 Genomic DNA. Translation: AAC73292.1 .
AP009048 Genomic DNA. Translation: BAA77856.2 .
PIRi E64742. XUECDP.
RefSeqi NP_414723.1. NC_000913.3.
YP_488483.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LXA X-ray 2.60 A 1-262 [» ]
2AQ9 X-ray 1.80 A 1-262 [» ]
2JF2 X-ray 1.80 A 1-262 [» ]
2JF3 X-ray 3.00 A 1-262 [» ]
2QIA X-ray 1.74 A 1-262 [» ]
2QIV X-ray 1.85 X 1-262 [» ]
4J09 X-ray 1.90 A 1-262 [» ]
ProteinModelPortali P0A722.
SMRi P0A722. Positions 1-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48043N.
IntActi P0A722. 36 interactions.
MINTi MINT-1234984.
STRINGi 511145.b0181.

Proteomic databases

PaxDbi P0A722.
PRIDEi P0A722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73292 ; AAC73292 ; b0181 .
BAA77856 ; BAA77856 ; BAA77856 .
GeneIDi 12932097.
944849.
KEGGi ecj:Y75_p0177.
eco:b0181.
PATRICi 32115473. VBIEscCol129921_0188.

Organism-specific databases

EchoBASEi EB0540.
EcoGenei EG10545. lpxA.

Phylogenomic databases

eggNOGi COG1043.
HOGENOMi HOG000294326.
InParanoidi P0A722.
KOi K00677.
OMAi DCQDKKY.
OrthoDBi EOG6F81P1.
PhylomeDBi P0A722.

Enzyme and pathway databases

UniPathwayi UPA00359 ; UER00477 .
BioCyci EcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.
ECOL316407:JW0176-MONOMER.
MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A722.
PROi P0A722.

Gene expression databases

Genevestigatori P0A722.

Family and domain databases

Gene3Di 1.20.1180.10. 1 hit.
HAMAPi MF_00387. LpxA.
InterProi IPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view ]
PIRSFi PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMi SSF51161. SSF51161. 1 hit.
TIGRFAMsi TIGR01852. lipid_A_lpxA. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene."
    Coleman J., Raetz C.R.H.
    J. Bacteriol. 170:1268-1274(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 64-65 AND 125.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase."
    Crowell D.N., Reznikoff W.S., Raetz C.R.H.
    J. Bacteriol. 169:5727-5734(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 148-262.
  7. "A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyltransferase."
    Raetz C.R.H., Roderick S.L.
    Science 270:997-1000(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
  8. "Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide."
    Williams A.H., Immormino R.M., Gewirth D.T., Raetz C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:10877-10882(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH INHIBITOR, SUBUNIT.
  9. "Nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis."
    Ulaganathan V., Buetow L., Hunter W.N.
    J. Mol. Biol. 369:305-312(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH UDP-N-ACETYL-GLUCOSAMINE, SUBUNIT.
  10. "Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase."
    Williams A.H., Raetz C.R.
    Proc. Natl. Acad. Sci. U.S.A. 104:13543-13550(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) IN COMPLEX WITH UDP-3-O-(R-3-HYDROXYMYRISTOYL)-GLCNAC, SUBUNIT.

Entry informationi

Entry nameiLPXA_ECOLI
AccessioniPrimary (citable) accession number: P0A722
Secondary accession number(s): P10440, P78243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3