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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125Substrate1
Binding sitei144Substrate1
Binding sitei161Substrate1

GO - Molecular functioni

  • acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: EcoliWiki
  • identical protein binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER
MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER
BRENDAi2.3.1.129 2026
UniPathwayiUPA00359; UER00477

Chemistry databases

SwissLipidsiSLP:000001883

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:b0181, JW0176
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10545 lpxA

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001880461 – 262Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST262

Proteomic databases

EPDiP0A722
PaxDbiP0A722
PRIDEiP0A722

Interactioni

Subunit structurei

Homotrimer.UniRule annotation3 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261091, 390 interactors
DIPiDIP-48043N
IntActiP0A722, 36 interactors
STRINGi316385.ECDH10B_0161

Structurei

Secondary structure

1262
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi12 – 14Combined sources3
Beta strandi36 – 38Combined sources3
Beta strandi52 – 56Combined sources5
Beta strandi67 – 70Combined sources4
Beta strandi83 – 86Combined sources4
Beta strandi97 – 99Combined sources3
Turni103 – 106Combined sources4
Beta strandi107 – 111Combined sources5
Beta strandi179 – 181Combined sources3
Beta strandi185 – 188Combined sources4
Turni189 – 192Combined sources4
Beta strandi193 – 197Combined sources5
Helixi199 – 204Combined sources6
Helixi209 – 223Combined sources15
Helixi229 – 240Combined sources12
Helixi244 – 246Combined sources3
Helixi247 – 255Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LXAX-ray2.60A1-262[»]
2AQ9X-ray1.80A1-262[»]
2JF2X-ray1.80A1-262[»]
2JF3X-ray3.00A1-262[»]
2QIAX-ray1.74A1-262[»]
2QIVX-ray1.85X1-262[»]
4J09X-ray1.90A1-262[»]
ProteinModelPortaliP0A722
SMRiP0A722
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A722

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 76Substrate binding4

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DAF Bacteria
COG1043 LUCA
HOGENOMiHOG000294326
InParanoidiP0A722
KOiK00677
OMAiRECVTIN
PhylomeDBiP0A722

Family and domain databases

CDDicd03351 LbH_UDP-GlcNAc_AT, 1 hit
Gene3Di1.20.1180.10, 1 hit
HAMAPiMF_00387 LpxA, 1 hit
InterProiView protein in InterPro
IPR029098 Acetyltransf_C
IPR037157 Acetyltransf_C_sf
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR010137 Lipid_A_LpxA
IPR011004 Trimer_LpxA-like_sf
PANTHERiPTHR43480 PTHR43480, 1 hit
PfamiView protein in Pfam
PF13720 Acetyltransf_11, 1 hit
PF00132 Hexapep, 2 hits
PIRSFiPIRSF000456 UDP-GlcNAc_acltr, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
TIGRFAMsiTIGR01852 lipid_A_lpxA, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 2 hits

Sequencei

Sequence statusi: Complete.

P0A722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV
60 70 80 90 100
NGHTKIGRDN EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR
110 120 130 140 150
GTVQGGGLTK VGSDNLLMIN AHIAHDCTVG NRCILANNAT LAGHVSVDDF
160 170 180 190 200
AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD VPPYVIAQGN HATPFGVNIE
210 220 230 240 250
GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA ETYPEVKAFT
260
DFFARSTRGL IR
Length:262
Mass (Da):28,080
Last modified:June 7, 2005 - v1
Checksum:iB42B076F0045B44C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64 – 65QF → SV in AAC36918 (PubMed:3277952).Curated2
Sequence conflicti125H → D in AAC36918 (PubMed:3277952).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti189G → S in strain: SM101; temperature-sensitive. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA Translation: AAC36918.1
U70214 Genomic DNA Translation: AAB08610.1
U00096 Genomic DNA Translation: AAC73292.1
AP009048 Genomic DNA Translation: BAA77856.2
PIRiE64742 XUECDP
RefSeqiNP_414723.1, NC_000913.3
WP_000565966.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73292; AAC73292; b0181
BAA77856; BAA77856; BAA77856
GeneIDi944849
KEGGiecj:JW0176
eco:b0181
PATRICifig|1411691.4.peg.2098

Similar proteinsi

Entry informationi

Entry nameiLPXA_ECOLI
AccessioniPrimary (citable) accession number: P0A722
Secondary accession number(s): P10440, P78243
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health