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Protein

Thymidylate kinase

Gene

tmk

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth (By similarity).By similarity

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12TMPBy similarity1
Binding sitei74TMPBy similarity1
Binding sitei78TMPBy similarity1
Binding sitei100TMPBy similarity1
Binding sitei105TMPBy similarity1
Binding sitei108TMPBy similarity1
Binding sitei109TMPBy similarity1
Sitei153Transition state stabilizerSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
Thymidine monophosphate kinase
dTMP kinase
Short name:
TMPK
Gene namesi
Name:tmk
Ordered Locus Names:SF1102, S1182
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB03280 P1-(5'-Adenosyl)P5-(5'-Thymidyl)Pentaphosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001553371 – 213Thymidylate kinaseAdd BLAST213

Proteomic databases

PRIDEiP0A721

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A721
SMRiP0A721
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 159LIDAdd BLAST13

Domaini

The LID domain is a solvent-exposed domain that closes over the site of phosphoryl transfer upon ATP binding.By similarity

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108ZMD Bacteria
COG0125 LUCA
HOGENOMiHOG000229078
KOiK00943
OMAiFLYTADH

Family and domain databases

HAMAPiMF_00165 Thymidylate_kinase, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR018095 Thymidylate_kin_CS
IPR018094 Thymidylate_kinase
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00041 DTMP_kinase, 1 hit
PROSITEiView protein in PROSITE
PS01331 THYMIDYLATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0A721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSKYIVIEG LEGAGKTTAR NVVVETLEQL GIRDMVFTRE PGGTQLAEKL
60 70 80 90 100
RSLVLDIKSV GDEVITDKAE VLMFYAARVQ LVETVIKPAL ANGTWVIGDR
110 120 130 140 150
HDLSTQAYQG GGRGIDQHML ATLRDAVLGD FRPDLTLYLD VTPEVGLKRA
160 170 180 190 200
RARGELDRIE QESFDFFNRT RARYLELAAQ DKSIHTIDAT QPLEAVMDAI
210
RTTVTHWVKE LDA
Length:213
Mass (Da):23,783
Last modified:March 15, 2005 - v1
Checksum:i16BC0A725AAFCB72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN42721.1
AE014073 Genomic DNA Translation: AAP16609.1
RefSeqiNP_707014.1, NC_004337.2
WP_001257000.1, NZ_NIYV01000083.1

Genome annotation databases

EnsemblBacteriaiAAN42721; AAN42721; SF1102
AAP16609; AAP16609; S1182
GeneIDi1024058
KEGGisfl:SF1102
sfx:S1182
PATRICifig|198214.7.peg.1290

Similar proteinsi

Entry informationi

Entry nameiKTHY_SHIFL
AccessioniPrimary (citable) accession number: P0A721
Secondary accession number(s): P37345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health