Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate kinase

Gene

tmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth.

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Kineticsi

  1. KM=0.04 mM for ATP (at pH 7.4 and 30 degrees Celsius)1 Publication
  2. KM=15 µM for dTMP (at pH 7.4 and 30 degrees Celsius)1 Publication
  1. Vmax=50 µmol/min/mg enzyme with ATP and dTMP as substrates (at pH 7.4 and 30 degrees Celsius)1 Publication

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12TMP1
Binding sitei74TMP1
Binding sitei78TMP1
Binding sitei100TMP1
Binding sitei105TMP1
Binding sitei108TMP1
Binding sitei109TMP1
Sitei153Transition state stabilizerSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • thymidylate kinase activity Source: EcoCyc
  • uridylate kinase activity Source: GO_Central

GO - Biological processi

  • dTDP biosynthetic process Source: EcoliWiki
  • dTTP biosynthetic process Source: EcoliWiki
  • dUDP biosynthetic process Source: GO_Central
  • nucleobase-containing small molecule interconversion Source: EcoliWiki

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DTMPKI-MONOMER
MetaCyc:DTMPKI-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
Thymidine monophosphate kinase
dTMP kinase
Short name:
TMPK
Gene namesi
Name:tmk
Synonyms:ycfG
Ordered Locus Names:b1098, JW1084
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12302 tmk

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001552691 – 213Thymidylate kinaseAdd BLAST213

Proteomic databases

PaxDbiP0A720
PRIDEiP0A720

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4263411, 41 interactors
DIPiDIP-48110N
IntActiP0A720, 8 interactors
STRINGi316385.ECDH10B_1170

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi16 – 29Combined sources14
Beta strandi35 – 41Combined sources7
Helixi45 – 55Combined sources11
Turni57 – 62Combined sources6
Helixi67 – 84Combined sources18
Helixi86 – 91Combined sources6
Beta strandi95 – 99Combined sources5
Helixi102 – 108Combined sources7
Turni109 – 113Combined sources5
Helixi117 – 128Combined sources12
Beta strandi134 – 140Combined sources7
Helixi143 – 153Combined sources11
Turni158 – 161Combined sources4
Helixi164 – 179Combined sources16
Beta strandi184 – 188Combined sources5
Helixi193 – 209Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9FX-ray1.90A151-156[»]
4TMKX-ray1.98A1-213[»]
5TMPX-ray1.98A1-213[»]
ProteinModelPortaliP0A720
SMRiP0A720
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A720

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 159LIDAdd BLAST13

Domaini

The LID domain is a solvent-exposed domain that closes over the site of phosphoryl transfer upon ATP binding.

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108ZMD Bacteria
COG0125 LUCA
HOGENOMiHOG000229078
InParanoidiP0A720
KOiK00943
OMAiFLYTADH
PhylomeDBiP0A720

Family and domain databases

HAMAPiMF_00165 Thymidylate_kinase, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR018095 Thymidylate_kin_CS
IPR018094 Thymidylate_kinase
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00041 DTMP_kinase, 1 hit
PROSITEiView protein in PROSITE
PS01331 THYMIDYLATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0A720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSKYIVIEG LEGAGKTTAR NVVVETLEQL GIRDMVFTRE PGGTQLAEKL
60 70 80 90 100
RSLVLDIKSV GDEVITDKAE VLMFYAARVQ LVETVIKPAL ANGTWVIGDR
110 120 130 140 150
HDLSTQAYQG GGRGIDQHML ATLRDAVLGD FRPDLTLYLD VTPEVGLKRA
160 170 180 190 200
RARGELDRIE QESFDFFNRT RARYLELAAQ DKSIHTIDAT QPLEAVMDAI
210
RTTVTHWVKE LDA
Length:213
Mass (Da):23,783
Last modified:March 15, 2005 - v1
Checksum:i16BC0A725AAFCB72
GO

Sequence cautioni

The sequence L01483 differs from that shown. Reason: Frameshift at positions 92 and 136.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112G → A (PubMed:8509334).Curated1
Sequence conflicti122T → N (PubMed:8509334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41456 Genomic DNA Translation: AAB06878.1
U00096 Genomic DNA Translation: AAC74182.1
AP009048 Genomic DNA Translation: BAA35905.1
L01483 Genomic DNA No translation available.
L04577 Genomic DNA No translation available.
PIRiJC6006 G64853
RefSeqiNP_415616.1, NC_000913.3
WP_001257000.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74182; AAC74182; b1098
BAA35905; BAA35905; BAA35905
GeneIDi945663
KEGGiecj:JW1084
eco:b1098
PATRICifig|1411691.4.peg.1170

Similar proteinsi

Entry informationi

Entry nameiKTHY_ECOLI
AccessioniPrimary (citable) accession number: P0A720
Secondary accession number(s): P37345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health