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P0A715

- KDSA_ECOLI

UniProt

P0A715 - KDSA_ECOLI

Protein

2-dehydro-3-deoxyphosphooctonate aldolase

Gene

kdsA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Synthesis of KDO 8-P which is required for lipid A maturation and cellular growth.

    Catalytic activityi

    Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate.

    Pathwayi

    GO - Molecular functioni

    1. 3-deoxy-8-phosphooctulonate synthase activity Source: EcoCyc

    GO - Biological processi

    1. keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:KDO-8PSYNTH-MONOMER.
    ECOL316407:JW1206-MONOMER.
    MetaCyc:KDO-8PSYNTH-MONOMER.
    SABIO-RKP0A715.
    UniPathwayiUPA00030.
    UPA00357; UER00474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase (EC:2.5.1.55)
    Alternative name(s):
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
    KDO-8-phosphate synthase
    Short name:
    KDO 8-P synthase
    Short name:
    KDOPS
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    Gene namesi
    Name:kdsA
    Ordered Locus Names:b1215, JW1206
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10518. kdsA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2842842-dehydro-3-deoxyphosphooctonate aldolasePRO_0000187124Add
    BLAST

    Proteomic databases

    PaxDbiP0A715.
    PRIDEiP0A715.

    2D gel databases

    SWISS-2DPAGEP0A715.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A715.

    Interactioni

    Subunit structurei

    Homotrimer.

    Protein-protein interaction databases

    DIPiDIP-35940N.
    IntActiP0A715. 46 interactions.
    MINTiMINT-1220797.
    STRINGi511145.b1215.

    Structurei

    Secondary structure

    1
    284
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 83
    Beta strandi11 – 133
    Beta strandi15 – 173
    Beta strandi20 – 223
    Beta strandi24 – 274
    Helixi31 – 4818
    Beta strandi55 – 584
    Beta strandi64 – 685
    Helixi74 – 8815
    Beta strandi93 – 953
    Turni99 – 1013
    Helixi102 – 1087
    Beta strandi110 – 1145
    Helixi116 – 1194
    Helixi122 – 13110
    Beta strandi134 – 1396
    Helixi145 – 1473
    Helixi148 – 1569
    Turni157 – 1593
    Beta strandi163 – 1675
    Beta strandi173 – 1753
    Helixi183 – 1908
    Turni191 – 1933
    Beta strandi196 – 1994
    Helixi201 – 2044
    Beta strandi211 – 2133
    Turni219 – 2224
    Helixi223 – 2319
    Beta strandi234 – 2374
    Beta strandi240 – 2423
    Helixi244 – 2474
    Beta strandi250 – 2523
    Helixi257 – 2593
    Helixi260 – 27516

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D9EX-ray2.40A/B/C/D1-284[»]
    1G7UX-ray2.80A1-284[»]
    1G7VX-ray2.40A1-284[»]
    1GG0X-ray3.00A1-284[»]
    1PHQX-ray2.70A1-284[»]
    1PHWX-ray2.36A1-284[»]
    1PL9X-ray2.90A1-284[»]
    1Q3NX-ray2.70A1-284[»]
    1X6UX-ray2.70A1-284[»]
    1X8FX-ray2.40A1-284[»]
    ProteinModelPortaliP0A715.
    SMRiP0A715. Positions 1-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A715.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the KdsA family.Curated

    Phylogenomic databases

    eggNOGiCOG2877.
    HOGENOMiHOG000023021.
    KOiK01627.
    OMAiANDKPMV.
    OrthoDBiEOG680X4R.
    PhylomeDBiP0A715.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00056. KDO8P_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006269. KDO8P_synthase.
    [Graphical view]
    PANTHERiPTHR21057:SF2. PTHR21057:SF2. 1 hit.
    PfamiPF00793. DAHP_synth_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01362. KDO8P_synth. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A715-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQKVVSIGD INVANDLPFV LFGGMNVLES RDLAMRICEH YVTVTQKLGI    50
    PYVFKASFDK ANRSSIHSYR GPGLEEGMKI FQELKQTFGV KIITDVHEPS 100
    QAQPVADVVD VIQLPAFLAR QTDLVEAMAK TGAVINVKKP QFVSPGQMGN 150
    IVDKFKEGGN EKVILCDRGA NFGYDNLVVD MLGFSIMKKV SGNSPVIFDV 200
    THALQCRDPF GAASGGRRAQ VAELARAGMA VGLAGLFIEA HPDPEHAKCD 250
    GPSALPLAKL EPFLKQMKAI DDLVKGFEEL DTSK 284
    Length:284
    Mass (Da):30,833
    Last modified:June 7, 2005 - v1
    Checksum:i35E79D692D134535
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221F → L in CAA29067. (PubMed:3039295)Curated

    Mass spectrometryi

    Molecular mass is 30842±17 Da from positions 1 - 284. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05552 Genomic DNA. Translation: CAA29067.1.
    U18555 Genomic DNA. Translation: AAC43441.1.
    U00096 Genomic DNA. Translation: AAC74299.1.
    AP009048 Genomic DNA. Translation: BAA36073.1.
    PIRiI83573. SYECOL.
    RefSeqiNP_415733.1. NC_000913.3.
    YP_489482.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74299; AAC74299; b1215.
    BAA36073; BAA36073; BAA36073.
    GeneIDi12931123.
    945785.
    KEGGiecj:Y75_p1187.
    eco:b1215.
    PATRICi32117682. VBIEscCol129921_1263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05552 Genomic DNA. Translation: CAA29067.1 .
    U18555 Genomic DNA. Translation: AAC43441.1 .
    U00096 Genomic DNA. Translation: AAC74299.1 .
    AP009048 Genomic DNA. Translation: BAA36073.1 .
    PIRi I83573. SYECOL.
    RefSeqi NP_415733.1. NC_000913.3.
    YP_489482.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D9E X-ray 2.40 A/B/C/D 1-284 [» ]
    1G7U X-ray 2.80 A 1-284 [» ]
    1G7V X-ray 2.40 A 1-284 [» ]
    1GG0 X-ray 3.00 A 1-284 [» ]
    1PHQ X-ray 2.70 A 1-284 [» ]
    1PHW X-ray 2.36 A 1-284 [» ]
    1PL9 X-ray 2.90 A 1-284 [» ]
    1Q3N X-ray 2.70 A 1-284 [» ]
    1X6U X-ray 2.70 A 1-284 [» ]
    1X8F X-ray 2.40 A 1-284 [» ]
    ProteinModelPortali P0A715.
    SMRi P0A715. Positions 1-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35940N.
    IntActi P0A715. 46 interactions.
    MINTi MINT-1220797.
    STRINGi 511145.b1215.

    2D gel databases

    SWISS-2DPAGE P0A715.

    Proteomic databases

    PaxDbi P0A715.
    PRIDEi P0A715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74299 ; AAC74299 ; b1215 .
    BAA36073 ; BAA36073 ; BAA36073 .
    GeneIDi 12931123.
    945785.
    KEGGi ecj:Y75_p1187.
    eco:b1215.
    PATRICi 32117682. VBIEscCol129921_1263.

    Organism-specific databases

    EchoBASEi EB0513.
    EcoGenei EG10518. kdsA.

    Phylogenomic databases

    eggNOGi COG2877.
    HOGENOMi HOG000023021.
    KOi K01627.
    OMAi ANDKPMV.
    OrthoDBi EOG680X4R.
    PhylomeDBi P0A715.

    Enzyme and pathway databases

    UniPathwayi UPA00030 .
    UPA00357 ; UER00474 .
    BioCyci EcoCyc:KDO-8PSYNTH-MONOMER.
    ECOL316407:JW1206-MONOMER.
    MetaCyc:KDO-8PSYNTH-MONOMER.
    SABIO-RK P0A715.

    Miscellaneous databases

    EvolutionaryTracei P0A715.
    PROi P0A715.

    Gene expression databases

    Genevestigatori P0A715.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00056. KDO8P_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006269. KDO8P_synthase.
    [Graphical view ]
    PANTHERi PTHR21057:SF2. PTHR21057:SF2. 1 hit.
    Pfami PF00793. DAHP_synth_1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01362. KDO8P_synth. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli."
      Woisetschlaeger M., Hoegenauer G.
      Mol. Gen. Genet. 207:369-373(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12."
      Strohmaier H., Remler P., Renner W., Hoegenauer G.
      J. Bacteriol. 177:4488-4500(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Strain: K12 / EMG2.
    7. "Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR."
      Dotson G.D., Dua R.K., Clemens J.C., Wooten E.W., Woodard R.W.
      J. Biol. Chem. 270:13698-13705(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1-10.
    8. "3-deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate."
      Wagner T., Kretsinger R.H., Bauerle R., Tolbert W.D.
      J. Mol. Biol. 301:233-238(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    9. "Crystal structures of KDOP synthase in its binary complexes with the substrate phosphoenolpyruvate and with a mechanism-based inhibitor."
      Asojo O., Friedman J., Adir N., Belakhov V., Shoham Y., Baasov T.
      Biochemistry 40:6326-6334(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), MASS SPECTROMETRY.

    Entry informationi

    Entry nameiKDSA_ECOLI
    AccessioniPrimary (citable) accession number: P0A715
    Secondary accession number(s): P17579
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3