Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Translation initiation factor IF-3

Gene

infC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the essential components for the initiation of protein synthesis.IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Important for 30S binding1 Publication1
Sitei110Important for 30S binding1 Publication1

GO - Molecular functioni

  • ribosome binding Source: EcoCyc
  • translation initiation factor activity Source: EcoCyc

GO - Biological processi

  • response to cold Source: EcoCyc
  • ribosome disassembly Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10506-MONOMER.
ECOL316407:JW5829-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-3UniRule annotation
Cleaved into the following 2 chains:
Translation initiation factor IF-3, N-terminally processedUniRule annotation
Translation initiation factor IF-3SUniRule annotation
Gene namesi
Name:infCUniRule annotation
Synonyms:fit, srjA
Ordered Locus Names:b1718, JW5829
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10506. infC.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107Y → F or L: Reduced ribosome binding. 1 Publication1
Mutagenesisi110K → R or L: Reduced ribosome binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075077.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003674981 – 180Translation initiation factor IF-31 PublicationAdd BLAST180
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00000144992 – 180Translation initiation factor IF-3, N-terminally processed1 PublicationAdd BLAST179
ChainiPRO_00003640897 – 180Translation initiation factor IF-3S1 PublicationAdd BLAST174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-methylmethionine; in Translation initiation factor IF-3; alternate1 Publication1

Post-translational modificationi

Phosphorylated on threonine residue(s).1 Publication
The form lacking the initiator methionine is less abundant than the N-methylmethionine form.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Methylation, Phosphoprotein

Proteomic databases

EPDiP0A707.
PaxDbiP0A707.
PRIDEiP0A707.

Interactioni

Subunit structurei

Monomer. Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsGP023593EBI-546262,EBI-543074

Protein-protein interaction databases

BioGridi4262192. 46 interactors.
DIPiDIP-36176N.
IntActiP0A707. 48 interactors.
MINTiMINT-1221578.
STRINGi511145.b1718.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi92 – 98Combined sources7
Helixi104 – 107Combined sources4
Helixi109 – 119Combined sources11
Beta strandi122 – 128Combined sources7
Helixi140 – 152Combined sources13
Turni153 – 155Combined sources3
Beta strandi156 – 159Combined sources4
Beta strandi171 – 176Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IFENMR-A81-180[»]
DisProtiDP00197.
ProteinModelPortaliP0A707.
SMRiP0A707.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A707.

Family & Domainsi

Sequence similaritiesi

Belongs to the IF-3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UUX. Bacteria.
COG0290. LUCA.
HOGENOMiHOG000035157.
InParanoidiP0A707.
KOiK02520.
OMAiHDFNVKV.
PhylomeDBiP0A707.

Family and domain databases

Gene3Di3.10.20.80. 1 hit.
3.30.110.10. 1 hit.
HAMAPiMF_00080. IF_3. 1 hit.
InterProiIPR019813. Translation_initiation_fac3_CS.
IPR001288. Translation_initiation_fac_3.
IPR019815. Translation_initiation_fac_3_C.
IPR019814. Translation_initiation_fac_3_N.
[Graphical view]
PANTHERiPTHR10938. PTHR10938. 1 hit.
PfamiPF00707. IF3_C. 1 hit.
PF05198. IF3_N. 1 hit.
[Graphical view]
SUPFAMiSSF54364. SSF54364. 1 hit.
SSF55200. SSF55200. 1 hit.
TIGRFAMsiTIGR00168. infC. 1 hit.
PROSITEiPS00938. IF3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGGKRVQTA RPNRINGEIR AQEVRLTGLE GEQLGIVSLR EALEKAEEAG
60 70 80 90 100
VDLVEISPNA EPPVCRIMDY GKFLYEKSKS SKEQKKKQKV IQVKEIKFRP
110 120 130 140 150
GTDEGDYQVK LRSLIRFLEE GDKAKITLRF RGREMAHQQI GMEVLNRVKD
160 170 180
DLQELAVVES FPTKIEGRQM IMVLAPKKKQ
Length:180
Mass (Da):20,564
Last modified:July 21, 1986 - v1
Checksum:i6120EB6AD42E9ABE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22Q → E AA sequence (PubMed:330233).Curated1
Sequence conflicti34 – 36LGI → IGMV AA sequence (PubMed:330233).Curated3
Sequence conflicti47E → Q AA sequence (PubMed:330233).Curated1
Sequence conflicti52D → N AA sequence (PubMed:330233).Curated1
Sequence conflicti61E → Q AA sequence (PubMed:330233).Curated1
Sequence conflicti87K → E AA sequence (PubMed:330233).Curated1
Sequence conflicti90V → K AA sequence (PubMed:330233).Curated1
Sequence conflicti103D → N AA sequence (PubMed:330233).Curated1
Sequence conflicti105G → N AA sequence (PubMed:330233).Curated1
Sequence conflicti161F → S in AAA51467 (Ref. 3) Curated1
Sequence conflicti178K → Q AA sequence (PubMed:330233).Curated1
Sequence conflicti180Missing AA sequence (PubMed:330233).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00291 Genomic DNA. Translation: CAA23561.1.
K02844 Genomic DNA. Translation: AAA51467.1.
U00096 Genomic DNA. Translation: AAC74788.1.
AP009048 Genomic DNA. Translation: BAA15485.1.
PIRiS13748. FIEC3.
RefSeqiNP_416233.1. NC_000913.3.
WP_001700733.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74788; AAC74788; b1718.
BAA15485; BAA15485; BAA15485.
GeneIDi946225.
KEGGiecj:JW5829.
eco:b1718.
PATRICi32118740. VBIEscCol129921_1788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00291 Genomic DNA. Translation: CAA23561.1.
K02844 Genomic DNA. Translation: AAA51467.1.
U00096 Genomic DNA. Translation: AAC74788.1.
AP009048 Genomic DNA. Translation: BAA15485.1.
PIRiS13748. FIEC3.
RefSeqiNP_416233.1. NC_000913.3.
WP_001700733.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IFENMR-A81-180[»]
DisProtiDP00197.
ProteinModelPortaliP0A707.
SMRiP0A707.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262192. 46 interactors.
DIPiDIP-36176N.
IntActiP0A707. 48 interactors.
MINTiMINT-1221578.
STRINGi511145.b1718.

Chemistry databases

ChEMBLiCHEMBL1075077.

Proteomic databases

EPDiP0A707.
PaxDbiP0A707.
PRIDEiP0A707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74788; AAC74788; b1718.
BAA15485; BAA15485; BAA15485.
GeneIDi946225.
KEGGiecj:JW5829.
eco:b1718.
PATRICi32118740. VBIEscCol129921_1788.

Organism-specific databases

EchoBASEiEB0501.
EcoGeneiEG10506. infC.

Phylogenomic databases

eggNOGiENOG4108UUX. Bacteria.
COG0290. LUCA.
HOGENOMiHOG000035157.
InParanoidiP0A707.
KOiK02520.
OMAiHDFNVKV.
PhylomeDBiP0A707.

Enzyme and pathway databases

BioCyciEcoCyc:EG10506-MONOMER.
ECOL316407:JW5829-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A707.
PROiP0A707.

Family and domain databases

Gene3Di3.10.20.80. 1 hit.
3.30.110.10. 1 hit.
HAMAPiMF_00080. IF_3. 1 hit.
InterProiIPR019813. Translation_initiation_fac3_CS.
IPR001288. Translation_initiation_fac_3.
IPR019815. Translation_initiation_fac_3_C.
IPR019814. Translation_initiation_fac_3_N.
[Graphical view]
PANTHERiPTHR10938. PTHR10938. 1 hit.
PfamiPF00707. IF3_C. 1 hit.
PF05198. IF3_N. 1 hit.
[Graphical view]
SUPFAMiSSF54364. SSF54364. 1 hit.
SSF55200. SSF55200. 1 hit.
TIGRFAMsiTIGR00168. infC. 1 hit.
PROSITEiPS00938. IF3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF3_ECOLI
AccessioniPrimary (citable) accession number: P0A707
Secondary accession number(s): P02999, P76905
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A short form called IF-3S/IF-3 beta is found both in vivo and in vitro and is probably produced by degradation of the long form IF-3L/IF-3 alpha. The major form is the N-methylmethionine long form.1 Publication

Caution

Was originally (PubMed:2954162) thought to control the translation of its own gene by binding to its mRNA; it now seems that discrimination against the AUU start codon is a kinetic effect (PubMed:16857585).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Translation initiation factors
    List of translation initiation factor entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.