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Protein

Hydrogenase maturation factor HybF

Gene

hybF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase. HybF is involved in maturation of hydrogenases 1 and 2. It may partially substitute for the function of HypA and vice versa.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2NickelUniRule annotation1 Publication1
Metal bindingi3NickelUniRule annotation1 Publication1
Metal bindingi73ZincUniRule annotation1 Publication1
Metal bindingi76ZincUniRule annotation1 Publication1
Metal bindingi89ZincUniRule annotation1 Publication1
Metal bindingi92ZincUniRule annotation1 Publication1

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • protein maturation Source: EcoCyc

Keywordsi

LigandMetal-binding, Nickel, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11804-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase maturation factor HybFUniRule annotationCurated
Alternative name(s):
Hydrogenase nickel incorporation protein HybFCurated
Hydrogenase-2 operon protein HybFCurated
Gene namesi
Name:hybF1 PublicationUniRule annotation
Ordered Locus Names:b2991, JW5493
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11804 hybF

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene severely reduces hydrogenase 1 and hydrogenase 2 activity (PubMed:12081959). HypA-hybF double mutant is completely blocked in maturation of hydrogenases 1, 2 and 3. However, the inclusion of high nickel concentrations in the medium can restore limited activity of all three hydrogenases (PubMed:12081959).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2H → A: Loss of activity. 1 Publication1
Mutagenesisi2H → Q: Loss of activity. Does not affect the amount of zinc, but shows a strong decrease in nickel content. 1 Publication1
Mutagenesisi3E → L: Loss of activity. 1 Publication1
Mutagenesisi3E → Q: Does not affect activity. 1 Publication1
Mutagenesisi73C → A: Affects stability and solubility of the protein, but mutant is still active. 1 Publication1
Mutagenesisi76C → A: Affects stability and solubility of the protein, but mutant is still active. 1 Publication1
Mutagenesisi89C → A or S: Affects stability and solubility of the protein, but mutant is still active. 1 Publication1
Mutagenesisi90P → A: Does not affect activity. 1 Publication1
Mutagenesisi92C → A: Affects stability and solubility of the protein, but mutant is still active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001290631 – 113Hydrogenase maturation factor HybFAdd BLAST113

Proteomic databases

PaxDbiP0A703
PRIDEiP0A703

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261537, 5 interactors
IntActiP0A703, 1 interactor
STRINGi316385.ECDH10B_3168

Structurei

3D structure databases

ProteinModelPortaliP0A703
SMRiP0A703
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HypA/HybF family. HybF subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105WMM Bacteria
COG0375 LUCA
HOGENOMiHOG000291317
InParanoidiP0A703
KOiK04651
OMAiMHEMSLC
PhylomeDBiP0A703

Family and domain databases

HAMAPiMF_02099 HybF_subfam, 1 hit
MF_00213 HypA_HybF, 1 hit
InterProiView protein in InterPro
IPR000688 Hydgase_Ni_incorp_HypA/HybF
IPR020538 Hydgase_Ni_incorp_HypA/HybF_CS
PANTHERiPTHR34535 PTHR34535, 1 hit
PfamiView protein in Pfam
PF01155 HypA, 1 hit
PIRSFiPIRSF004761 Hydrgn_mat_HypA, 1 hit
TIGRFAMsiTIGR00100 hypA, 1 hit
PROSITEiView protein in PROSITE
PS01249 HYPA, 1 hit

Sequencei

Sequence statusi: Complete.

P0A703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHELSLCQSA VEIIQRQAEQ HDVKRVTAVW LEIGALSCVE ESAVRFSFEI
60 70 80 90 100
VCHGTVAQGC DLHIVYKPAQ AWCWDCSQVV EIHQHDAQCP LCHGERLRVD
110
TGDSLIVKSI EVE
Length:113
Mass (Da):12,697
Last modified:June 7, 2005 - v1
Checksum:i70919C4A40CEABE6
GO

Sequence cautioni

The sequence AAA21594 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA Translation: AAA21594.1 Sequence problems.
U28377 Genomic DNA Translation: AAA69158.1
U00096 Genomic DNA Translation: AAC76027.1
AP009048 Genomic DNA Translation: BAE77052.1
PIRiF55516
RefSeqiNP_417465.1, NC_000913.3
WP_000544953.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76027; AAC76027; b2991
BAE77052; BAE77052; BAE77052
GeneIDi948004
KEGGiecj:JW5493
eco:b2991
PATRICifig|1411691.4.peg.3738

Similar proteinsi

Entry informationi

Entry nameiHYBF_ECOLI
AccessioniPrimary (citable) accession number: P0A703
Secondary accession number(s): P37184, Q2M9K4, Q46846
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: April 25, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health