P0A703 (HYBF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable hydrogenase nickel incorporation protein HybF

Alternative name(s):
Hydrogenase-2 operon protein HybF

Gene names

Name:	hybF
Ordered Locus Names:	b2991, JW5493

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably plays a role in a hydrogenase nickel cofactor insertion step. HAMAP-Rule MF_00213
Sequence similarities	Belongs to the HypA/HybF family.
Sequence caution	The sequence AAA21594.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from electronic annotation. Source: HAMAP protein maturation Inferred from mutant phenotype PubMed 12081959. Source: EcoCyc
Molecular_function	nickel cation binding Inferred from direct assay PubMed 15090500. Source: EcoCyc zinc ion binding Inferred from direct assay PubMed 15090500. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 113	113	Probable hydrogenase nickel incorporation protein HybF HAMAP-Rule MF_00213		PRO_0000129063
Sites
	Metal binding	2	1	Nickel Potential
	Metal binding	73	1	Nickel Potential
	Metal binding	76	1	Nickel Potential
	Metal binding	89	1	Nickel Potential
	Metal binding	92	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A703 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 70919C4A40CEABE6	FASTA	113	12,697
	10 20 30 40 50 60 MHELSLCQSA VEIIQRQAEQ HDVKRVTAVW LEIGALSCVE ESAVRFSFEI VCHGTVAQGC 70 80 90 100 110 DLHIVYKPAQ AWCWDCSQVV EIHQHDAQCP LCHGERLRVD TGDSLIVKSI EVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E. J. Bacteriol. 176:4416-4423(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / TG1.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09177 Genomic DNA. Translation: AAA21594.1. Sequence problems. U28377 Genomic DNA. Translation: AAA69158.1. U00096 Genomic DNA. Translation: AAC76027.1. AP009048 Genomic DNA. Translation: BAE77052.1.
PIR	F55516.
RefSeq	NP_417465.1. NC_000913.2. YP_491188.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A703.
SMR	P0A703. Positions 2-113.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b2991.
Proteomic databases
PRIDE	P0A703.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76027; AAC76027; b2991. BAE77052; BAE77052; BAE77052.
GeneID	12932543. 948004.
KEGG	ecj:Y75_p2920. eco:b2991.
PATRIC	32121398. VBIEscCol129921_3086.
Organism-specific databases
EchoBASE	EB1752.
EcoGene	EG11804. hybF.
Phylogenomic databases
eggNOG	COG0375.
HOGENOM	HOG000291317.
KO	K04651.
OMA	MHEMSLC.
ProtClustDB	PRK12380.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11804-MONOMER. ECOL316407:JW5493-MONOMER.
Gene expression databases
Genevestigator	P0A703.
Family and domain databases
HAMAP	MF_00213. HypA.
InterPro	IPR000688. Hydgase_Ni_incorp_HypA/HybF. IPR020538. Hydgase_Ni_incorp_HypA/HybF_CS. [Graphical view]
Pfam	PF01155. HypA. 1 hit. [Graphical view]
PIRSF	PIRSF004761. Hydrgn_mat_HypA. 1 hit.
TIGRFAMs	TIGR00100. hypA. 1 hit.
PROSITE	PS01249. HYPA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYBF_ECOLI

Accession

Primary (citable) accession number: P0A703
Secondary accession number(s): P37184, Q2M9K4, Q46846

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program