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Protein

Probable hydrogenase nickel incorporation protein HybF

Gene

hybF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably plays a role in a hydrogenase nickel cofactor insertion step.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi2 – 21NickelSequence analysis
Metal bindingi73 – 731NickelSequence analysis
Metal bindingi76 – 761NickelSequence analysis
Metal bindingi89 – 891NickelSequence analysis
Metal bindingi92 – 921NickelSequence analysis

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • cellular protein modification process Source: UniProtKB-HAMAP
  • protein maturation Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:EG11804-MONOMER.
ECOL316407:JW5493-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable hydrogenase nickel incorporation protein HybF
Alternative name(s):
Hydrogenase-2 operon protein HybF
Gene namesi
Name:hybF
Ordered Locus Names:b2991, JW5493
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11804. hybF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 113113Probable hydrogenase nickel incorporation protein HybFPRO_0000129063Add
BLAST

Proteomic databases

PaxDbiP0A703.
PRIDEiP0A703.

Interactioni

Protein-protein interaction databases

BioGridi4261537. 5 interactions.
IntActiP0A703. 1 interaction.
STRINGi511145.b2991.

Structurei

3D structure databases

ProteinModelPortaliP0A703.
SMRiP0A703. Positions 2-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HypA/HybF family.Curated

Phylogenomic databases

eggNOGiENOG4105WMM. Bacteria.
COG0375. LUCA.
HOGENOMiHOG000291317.
InParanoidiP0A703.
KOiK04651.
OMAiMHEMSLC.
PhylomeDBiP0A703.

Family and domain databases

HAMAPiMF_00213. HypA. 1 hit.
InterProiIPR000688. Hydgase_Ni_incorp_HypA/HybF.
IPR020538. Hydgase_Ni_incorp_HypA/HybF_CS.
[Graphical view]
PfamiPF01155. HypA. 1 hit.
[Graphical view]
PIRSFiPIRSF004761. Hydrgn_mat_HypA. 1 hit.
TIGRFAMsiTIGR00100. hypA. 1 hit.
PROSITEiPS01249. HYPA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHELSLCQSA VEIIQRQAEQ HDVKRVTAVW LEIGALSCVE ESAVRFSFEI
60 70 80 90 100
VCHGTVAQGC DLHIVYKPAQ AWCWDCSQVV EIHQHDAQCP LCHGERLRVD
110
TGDSLIVKSI EVE
Length:113
Mass (Da):12,697
Last modified:June 7, 2005 - v1
Checksum:i70919C4A40CEABE6
GO

Sequence cautioni

The sequence AAA21594 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21594.1. Sequence problems.
U28377 Genomic DNA. Translation: AAA69158.1.
U00096 Genomic DNA. Translation: AAC76027.1.
AP009048 Genomic DNA. Translation: BAE77052.1.
PIRiF55516.
RefSeqiNP_417465.1. NC_000913.3.
WP_000544953.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76027; AAC76027; b2991.
BAE77052; BAE77052; BAE77052.
GeneIDi948004.
KEGGiecj:JW5493.
eco:b2991.
PATRICi32121398. VBIEscCol129921_3086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21594.1. Sequence problems.
U28377 Genomic DNA. Translation: AAA69158.1.
U00096 Genomic DNA. Translation: AAC76027.1.
AP009048 Genomic DNA. Translation: BAE77052.1.
PIRiF55516.
RefSeqiNP_417465.1. NC_000913.3.
WP_000544953.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A703.
SMRiP0A703. Positions 2-113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261537. 5 interactions.
IntActiP0A703. 1 interaction.
STRINGi511145.b2991.

Proteomic databases

PaxDbiP0A703.
PRIDEiP0A703.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76027; AAC76027; b2991.
BAE77052; BAE77052; BAE77052.
GeneIDi948004.
KEGGiecj:JW5493.
eco:b2991.
PATRICi32121398. VBIEscCol129921_3086.

Organism-specific databases

EchoBASEiEB1752.
EcoGeneiEG11804. hybF.

Phylogenomic databases

eggNOGiENOG4105WMM. Bacteria.
COG0375. LUCA.
HOGENOMiHOG000291317.
InParanoidiP0A703.
KOiK04651.
OMAiMHEMSLC.
PhylomeDBiP0A703.

Enzyme and pathway databases

BioCyciEcoCyc:EG11804-MONOMER.
ECOL316407:JW5493-MONOMER.

Miscellaneous databases

PROiP0A703.

Family and domain databases

HAMAPiMF_00213. HypA. 1 hit.
InterProiIPR000688. Hydgase_Ni_incorp_HypA/HybF.
IPR020538. Hydgase_Ni_incorp_HypA/HybF_CS.
[Graphical view]
PfamiPF01155. HypA. 1 hit.
[Graphical view]
PIRSFiPIRSF004761. Hydrgn_mat_HypA. 1 hit.
TIGRFAMsiTIGR00100. hypA. 1 hit.
PROSITEiPS01249. HYPA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYBF_ECOLI
AccessioniPrimary (citable) accession number: P0A703
Secondary accession number(s): P37184, Q2M9K4, Q46846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.