P0A700 (HYPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein HypA

Gene names

Name:	hypA
Ordered Locus Names:	b2726, JW2696

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not required for the formation of hydrogenases 1 and 2. Plays probably a regulatory role. HAMAP-Rule MF_00213
Sequence similarities	Belongs to the HypA/HybF family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from electronic annotation. Source: HAMAP protein maturation Inferred from mutant phenotype PubMed 12081959. Source: EcoCyc
Cellular_component	extrinsic to internal side of plasma membrane Inferred from direct assay PubMed 22016389. Source: EcoCyc
Molecular_function	nickel cation binding Inferred from direct assay PubMed 15995183. Source: EcoCyc zinc ion binding Inferred from direct assay PubMed 15995183. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
hycE	P16431	14	EBI-6290024,EBI-552702

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	Protein HypA HAMAP-Rule MF_00213		PRO_0000129057
Sites
	Metal binding	2	1	Nickel Potential
	Metal binding	73	1	Nickel Potential
	Metal binding	76	1	Nickel Potential
	Metal binding	90	1	Nickel Potential
	Metal binding	93	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A700 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 57458941783073D4	FASTA	116	13,168
	10 20 30 40 50 60 MHEITLCQRA LELIEQQAAK HGAKRVTGVW LKIGAFSCVE TSSLAFCFDL VCRGSVAEGC 70 80 90 100 110 KLHLEEQEAE CWCETCQQYV TLLTQRVRRC PQCHGDMLQI VADDGLQIRR IEIDQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli." Lutz S., Jacobi A., Schlensog V., Boehm R., Sawers G., Boeck A. Mol. Microbiol. 5:123-135(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54543 Genomic DNA. Translation: CAA38412.1. U29579 Genomic DNA. Translation: AAA69236.1. U00096 Genomic DNA. Translation: AAC75768.1. AP009048 Genomic DNA. Translation: BAE76803.1.
PIR	S15197.
RefSeq	NP_417206.1. NC_000913.2. YP_490935.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A700.
SMR	P0A700. Positions 2-116.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48022N.
IntAct	P0A700. 7 interactions.
STRING	511145.b2726.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75768; AAC75768; b2726. BAE76803; BAE76803; BAE76803.
GeneID	12932434. 947195.
KEGG	ecj:Y75_p2664. eco:b2726.
PATRIC	32120852. VBIEscCol129921_2818.
Organism-specific databases
EchoBASE	EB0478.
EcoGene	EG10483. hypA.
Phylogenomic databases
eggNOG	COG0375.
HOGENOM	HOG000291317.
KO	K04651.
OMA	AFSCVEP.
ProtClustDB	PRK03681.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10483-MONOMER. ECOL316407:JW2696-MONOMER. MetaCyc:EG10483-MONOMER.
Gene expression databases
Genevestigator	P0A700.
Family and domain databases
HAMAP	MF_00213. HypA.
InterPro	IPR000688. Hydgase_Ni_incorp_HypA/HybF. IPR020538. Hydgase_Ni_incorp_HypA/HybF_CS. [Graphical view]
Pfam	PF01155. HypA. 1 hit. [Graphical view]
PIRSF	PIRSF004761. Hydrgn_mat_HypA. 1 hit.
TIGRFAMs	TIGR00100. hypA. 1 hit.
PROSITE	PS01249. HYPA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYPA_ECOLI

Accession

Primary (citable) accession number: P0A700
Secondary accession number(s): P24189, Q2MAA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program