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Protein

Hydrogenase maturation factor HypA

Gene

hypA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase (PubMed:12081959, PubMed:15995183). Mediates transfer of nickel, but not zinc, from the low-affinity metal-binding site in the GTPase domain of HypB to HypA (PubMed:23899293, PubMed:27951644). HypA is involved in maturation of hydrogenase 3. It may partially substitute for the function of HybF and vice versa (PubMed:12081959). May act as a scaffold for assembly of the nickel insertion proteins with the hydrogenase precursor protein after delivery of the iron center (PubMed:22016389).5 Publications

Enzyme regulationi

GDP-loaded state of HypB enhances HypA-HypB complex formation and nickel transfer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2NickelUniRule annotation1 Publication1
Metal bindingi73ZincUniRule annotation1
Metal bindingi76ZincUniRule annotation1
Metal bindingi90ZincUniRule annotation1
Metal bindingi93ZincUniRule annotation1

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • protein maturation Source: EcoCyc

Keywordsi

LigandMetal-binding, Nickel, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10483-MONOMER
MetaCyc:EG10483-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase maturation factor HypACurated
Gene namesi
Name:hypA1 Publication
Ordered Locus Names:b2726, JW2696
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10483 hypA

Subcellular locationi

  • Cell inner membrane 1 Publication

GO - Cellular componenti

  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of the gene prevents development of hydrogenase 3 activity, does not influence the level of hydrogenase 1 and leads to a considerable increase in hydrogenase 2 activity (PubMed:1482271). HypA-hybF double mutant is completely blocked in maturation of hydrogenases 1, 2 and 3. However, the inclusion of high nickel concentrations in the medium can restore limited activity of all three hydrogenases (PubMed:12081959).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2H → A: Does not affect interaction with HycE. 1 Publication1
Mutagenesisi2H → Q: Can bind nickel, but with reduced affinity. Decreases nickel transfer from HypB and cannot support hydrogenase maturation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001290571 – 116Hydrogenase maturation factor HypAAdd BLAST116

Proteomic databases

PaxDbiP0A700
PRIDEiP0A700

Interactioni

Subunit structurei

Homodimer (PubMed:15995183). Forms complexes with HypB and SlyD, and interacts with HycE, the large subunit of hydrogenase 3 (PubMed:15995183, PubMed:22016389, PubMed:23899293).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
hycEP1643114EBI-6290024,EBI-552702

Protein-protein interaction databases

BioGridi4261428, 8 interactors
DIPiDIP-48022N
IntActiP0A700, 9 interactors
STRINGi316385.ECDH10B_2894

Structurei

3D structure databases

ProteinModelPortaliP0A700
SMRiP0A700
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HypA/HybF family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG410816D Bacteria
COG0375 LUCA
HOGENOMiHOG000291317
InParanoidiP0A700
KOiK04651
OMAiHEYSIVT
PhylomeDBiP0A700

Family and domain databases

HAMAPiMF_00213 HypA_HybF, 1 hit
InterProiView protein in InterPro
IPR020538 Hydgase_Ni_incorp_HypA/HybF_CS
IPR000688 HypA/HybF
PANTHERiPTHR34535 PTHR34535, 1 hit
PfamiView protein in Pfam
PF01155 HypA, 1 hit
PIRSFiPIRSF004761 Hydrgn_mat_HypA, 1 hit
TIGRFAMsiTIGR00100 hypA, 1 hit
PROSITEiView protein in PROSITE
PS01249 HYPA, 1 hit

Sequencei

Sequence statusi: Complete.

P0A700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEITLCQRA LELIEQQAAK HGAKRVTGVW LKIGAFSCVE TSSLAFCFDL
60 70 80 90 100
VCRGSVAEGC KLHLEEQEAE CWCETCQQYV TLLTQRVRRC PQCHGDMLQI
110
VADDGLQIRR IEIDQE
Length:116
Mass (Da):13,168
Last modified:June 7, 2005 - v1
Checksum:i57458941783073D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA Translation: CAA38412.1
U29579 Genomic DNA Translation: AAA69236.1
U00096 Genomic DNA Translation: AAC75768.1
AP009048 Genomic DNA Translation: BAE76803.1
PIRiS15197
RefSeqiNP_417206.1, NC_000913.3
WP_001299100.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75768; AAC75768; b2726
BAE76803; BAE76803; BAE76803
GeneIDi947195
KEGGiecj:JW2696
eco:b2726
PATRICifig|1411691.4.peg.4015

Similar proteinsi

Entry informationi

Entry nameiHYPA_ECOLI
AccessioniPrimary (citable) accession number: P0A700
Secondary accession number(s): P24189, Q2MAA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health