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Protein

Chaperone protein HtpG

Gene

htpG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone. Has ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATP1 Publication1
Binding sitei80ATP1 Publication1
Binding sitei127ATP; via amide nitrogen1 Publication1
Binding sitei174ATP1 Publication1
Binding sitei255ATP1 Publication1

GO - Molecular functioni

  • ATPase activity Source: CACAO
  • ATPase activity, coupled Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • protein folding Source: EcoCyc
  • response to heat Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10461-MONOMER.
ECOL316407:JW0462-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein HtpG
Alternative name(s):
Heat shock protein C62.5
Heat shock protein HtpG
High temperature protein G
Gene namesi
Name:htpG
Ordered Locus Names:b0473, JW0462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10461. htpG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000629851 – 624Chaperone protein HtpGAdd BLAST624

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0A6Z3.
PaxDbiP0A6Z3.
PRIDEiP0A6Z3.

2D gel databases

SWISS-2DPAGEP0A6Z3.

Interactioni

Subunit structurei

Homodimer. Oligomerizes at 65 degrees Celsius.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-369221,EBI-369221
dnaKP0A6Y83EBI-369221,EBI-542092
rpoCP0A8T72EBI-369221,EBI-543604

Protein-protein interaction databases

BioGridi4261971. 197 interactors.
DIPiDIP-29797N.
IntActiP0A6Z3. 61 interactors.
MINTiMINT-1227806.
STRINGi511145.b0473.

Structurei

Secondary structure

1624
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi11 – 23Combined sources13
Helixi25 – 29Combined sources5
Helixi30 – 50Combined sources21
Helixi54 – 57Combined sources4
Beta strandi65 – 70Combined sources6
Turni71 – 74Combined sources4
Beta strandi75 – 80Combined sources6
Helixi87 – 94Combined sources8
Helixi102 – 108Combined sources7
Helixi113 – 120Combined sources8
Helixi127 – 132Combined sources6
Beta strandi134 – 142Combined sources9
Beta strandi144 – 146Combined sources3
Helixi148 – 150Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi158 – 167Combined sources10
Beta strandi173 – 180Combined sources8
Helixi185 – 188Combined sources4
Helixi190 – 200Combined sources11
Beta strandi208 – 210Combined sources3
Beta strandi221 – 226Combined sources6
Helixi233 – 235Combined sources3
Helixi238 – 240Combined sources3
Helixi243 – 254Combined sources12
Beta strandi261 – 268Combined sources8
Beta strandi270 – 272Combined sources3
Beta strandi274 – 280Combined sources7
Turni286 – 289Combined sources4
Beta strandi296 – 301Combined sources6
Beta strandi304 – 310Combined sources7
Helixi311 – 313Combined sources3
Helixi316 – 318Combined sources3
Beta strandi322 – 330Combined sources9
Helixi336 – 341Combined sources6
Helixi343 – 366Combined sources24
Helixi368 – 382Combined sources15
Helixi385 – 388Combined sources4
Helixi390 – 392Combined sources3
Helixi393 – 397Combined sources5
Beta strandi402 – 404Combined sources3
Turni405 – 408Combined sources4
Helixi416 – 421Combined sources6
Beta strandi429 – 434Combined sources6
Helixi438 – 442Combined sources5
Helixi445 – 447Combined sources3
Helixi448 – 453Combined sources6
Beta strandi457 – 460Combined sources4
Helixi465 – 468Combined sources4
Turni469 – 471Combined sources3
Beta strandi474 – 477Combined sources4
Beta strandi478 – 482Combined sources5
Helixi488 – 493Combined sources6
Helixi511 – 519Combined sources9
Helixi520 – 522Combined sources3
Beta strandi523 – 528Combined sources6
Beta strandi537 – 540Combined sources4
Helixi548 – 555Combined sources8
Turni556 – 558Combined sources3
Beta strandi567 – 570Combined sources4
Helixi575 – 582Combined sources8
Helixi586 – 605Combined sources20
Helixi611 – 623Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SF8X-ray2.60A/B/C/D/E/F/G/H511-624[»]
1Y4SX-ray2.90A/B1-559[»]
1Y4UX-ray2.90A/B1-559[»]
2GQ0X-ray1.90A/B230-495[»]
2IOPX-ray3.55A/B/C/D1-624[»]
2IOQX-ray3.50A/B1-624[»]
2IORX-ray1.65A1-215[»]
ProteinModelPortaliP0A6Z3.
SMRiP0A6Z3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6Z3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 336A; substrate-bindingAdd BLAST336
Regioni337 – 552BAdd BLAST216
Regioni553 – 624CAdd BLAST72
Regioni585 – 624Mediates dimerizationAdd BLAST40

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Phylogenomic databases

eggNOGiENOG4105CJX. Bacteria.
COG0326. LUCA.
HOGENOMiHOG000031989.
InParanoidiP0A6Z3.
KOiK04079.
OMAiYLRFMRG.
PhylomeDBiP0A6Z3.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGQETRGFQ SEVKQLLHLM IHSLYSNKEI FLRELISNAS DAADKLRFRA
60 70 80 90 100
LSNPDLYEGD GELRVRVSFD KDKRTLTISD NGVGMTRDEV IDHLGTIAKS
110 120 130 140 150
GTKSFLESLG SDQAKDSQLI GQFGVGFYSA FIVADKVTVR TRAAGEKPEN
160 170 180 190 200
GVFWESAGEG EYTVADITKE DRGTEITLHL REGEDEFLDD WRVRSIISKY
210 220 230 240 250
SDHIALPVEI EKREEKDGET VISWEKINKA QALWTRNKSE ITDEEYKEFY
260 270 280 290 300
KHIAHDFNDP LTWSHNRVEG KQEYTSLLYI PSQAPWDMWN RDHKHGLKLY
310 320 330 340 350
VQRVFIMDDA EQFMPNYLRF VRGLIDSSDL PLNVSREILQ DSTVTRNLRN
360 370 380 390 400
ALTKRVLQML EKLAKDDAEK YQTFWQQFGL VLKEGPAEDF ANQEAIAKLL
410 420 430 440 450
RFASTHTDSS AQTVSLEDYV SRMKEGQEKI YYITADSYAA AKSSPHLELL
460 470 480 490 500
RKKGIEVLLL SDRIDEWMMN YLTEFDGKPF QSVSKVDESL EKLADEVDES
510 520 530 540 550
AKEAEKALTP FIDRVKALLG ERVKDVRLTH RLTDTPAIVS TDADEMSTQM
560 570 580 590 600
AKLFAAAGQK VPEVKYIFEL NPDHVLVKRA ADTEDEAKFS EWVELLLDQA
610 620
LLAERGTLED PNLFIRRMNQ LLVS
Length:624
Mass (Da):71,423
Last modified:June 7, 2005 - v1
Checksum:i1410577B589ADBAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38777 Genomic DNA. Translation: AAA23460.1.
U82664 Genomic DNA. Translation: AAB40227.1.
U00096 Genomic DNA. Translation: AAC73575.1.
AP009048 Genomic DNA. Translation: BAE76252.1.
PIRiA28324. HHEC62.
RefSeqiNP_415006.1. NC_000913.3.
WP_000678201.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC73575; AAC73575; b0473.
BAE76252; BAE76252; BAE76252.
GeneIDi945099.
KEGGiecj:JW0462.
eco:b0473.
PATRICi32116103. VBIEscCol129921_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38777 Genomic DNA. Translation: AAA23460.1.
U82664 Genomic DNA. Translation: AAB40227.1.
U00096 Genomic DNA. Translation: AAC73575.1.
AP009048 Genomic DNA. Translation: BAE76252.1.
PIRiA28324. HHEC62.
RefSeqiNP_415006.1. NC_000913.3.
WP_000678201.1. NZ_CP014272.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SF8X-ray2.60A/B/C/D/E/F/G/H511-624[»]
1Y4SX-ray2.90A/B1-559[»]
1Y4UX-ray2.90A/B1-559[»]
2GQ0X-ray1.90A/B230-495[»]
2IOPX-ray3.55A/B/C/D1-624[»]
2IOQX-ray3.50A/B1-624[»]
2IORX-ray1.65A1-215[»]
ProteinModelPortaliP0A6Z3.
SMRiP0A6Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261971. 197 interactors.
DIPiDIP-29797N.
IntActiP0A6Z3. 61 interactors.
MINTiMINT-1227806.
STRINGi511145.b0473.

2D gel databases

SWISS-2DPAGEP0A6Z3.

Proteomic databases

EPDiP0A6Z3.
PaxDbiP0A6Z3.
PRIDEiP0A6Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73575; AAC73575; b0473.
BAE76252; BAE76252; BAE76252.
GeneIDi945099.
KEGGiecj:JW0462.
eco:b0473.
PATRICi32116103. VBIEscCol129921_0493.

Organism-specific databases

EchoBASEiEB0456.
EcoGeneiEG10461. htpG.

Phylogenomic databases

eggNOGiENOG4105CJX. Bacteria.
COG0326. LUCA.
HOGENOMiHOG000031989.
InParanoidiP0A6Z3.
KOiK04079.
OMAiYLRFMRG.
PhylomeDBiP0A6Z3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10461-MONOMER.
ECOL316407:JW0462-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6Z3.
PROiP0A6Z3.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00298. HSP90. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHTPG_ECOLI
AccessioniPrimary (citable) accession number: P0A6Z3
Secondary accession number(s): P10413, Q2MBV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.