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Protein

Chaperone protein HscA

Gene

hscA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU.

GO - Molecular functioni

  • ADP binding Source: EcoCyc
  • ATPase activity Source: EcoCyc
  • ATP binding Source: EcoCyc

GO - Biological processi

  • cellular response to cold Source: EcoCyc
  • iron-sulfur cluster assembly Source: InterPro
  • protein folding Source: UniProtKB-HAMAP
  • protein maturation by iron-sulfur cluster transfer Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12130-MONOMER.
ECOL316407:JW2510-MONOMER.
MetaCyc:EG12130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein HscA
Alternative name(s):
Hsc66
Gene namesi
Name:hscA
Synonyms:hsc
Ordered Locus Names:b2526, JW2510
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12130. hscA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • iron-sulfur cluster transfer complex Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 616616Chaperone protein HscAPRO_0000078625Add
BLAST

Proteomic databases

EPDiP0A6Z1.
PaxDbiP0A6Z1.
PRIDEiP0A6Z1.

Expressioni

Inductioni

By cold shock.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262906. 73 interactions.
DIPiDIP-47348N.
IntActiP0A6Z1. 28 interactions.
STRINGi511145.b2526.

Structurei

Secondary structure

1
616
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi396 – 4005Combined sources
Turni401 – 4033Combined sources
Beta strandi404 – 4096Combined sources
Beta strandi414 – 42411Combined sources
Beta strandi433 – 4397Combined sources
Beta strandi441 – 4444Combined sources
Helixi445 – 4473Combined sources
Beta strandi448 – 4569Combined sources
Beta strandi469 – 4757Combined sources
Beta strandi481 – 4877Combined sources
Turni488 – 4903Combined sources
Beta strandi493 – 4986Combined sources
Helixi506 – 51813Combined sources
Helixi520 – 55031Combined sources
Helixi551 – 5533Combined sources
Helixi556 – 57217Combined sources
Helixi578 – 60932Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U00X-ray1.95A389-615[»]
ProteinModelPortaliP0A6Z1.
SMRiP0A6Z1. Positions 23-615.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6Z1.

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105C9I. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
InParanoidiP0A6Z1.
KOiK04044.
OMAiLRGIPAM.
PhylomeDBiP0A6Z1.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00679. HscA. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
IPR010236. ISC_FeS_clus_asmbl_HscA.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR01991. HscA. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLADHEGR
60 70 80 90 100
HLLPSVVHYQ QQGHSVGYDA RTNAALDTAN TISSVKRLMG RSLADIQQRY
110 120 130 140 150
PHLPYQFQAS ENGLPMIETA AGLLNPVRVS ADILKALAAR ATEALAGELD
160 170 180 190 200
GVVITVPAYF DDAQRQGTKD AARLAGLHVL RLLNEPTAAA IAYGLDSGQE
210 220 230 240 250
GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD FDHLLADYIR
260 270 280 290 300
EQAGIPDRSD NRVQRELLDA AIAAKIALSD ADSVTVNVAG WQGEISREQF
310 320 330 340 350
NELIAPLVKR TLLACRRALK DAGVEADEVL EVVMVGGSTR VPLVRERVGE
360 370 380 390 400
FFGRPPLTSI DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET
410 420 430 440 450
MGGLVEKVIP RNTTIPVARA QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS
460 470 480 490 500
LARFALRGIP ALPAGGAHIR VTFQVDADGL LSVTAMEKST GVEASIQVKP
510 520 530 540 550
SYGLTDSEIA SMIKDSMSYA EQDVKARMLA EQKVEAARVL ESLHGALAAD
560 570 580 590 600
AALLSAAERQ VIDDAAAHLS EVAQGDDVDA IEQAIKNVDK QTQDFAARRM
610
DQSVRRALKG HSVDEV
Length:616
Mass (Da):65,652
Last modified:June 7, 2005 - v1
Checksum:i9C80E6EF1BFDAB48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti456 – 46510LRGIPALPAG → CVVFRRYRLA in AAA18300 (PubMed:8300516).Curated
Sequence conflicti516 – 5161S → T in AAA18300 (PubMed:8300516).Curated
Sequence conflicti557 – 5571Missing in AAA18300 (PubMed:8300516).Curated
Sequence conflicti583 – 5831Q → K in AAD13473 (PubMed:8134349).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti216 – 2161S → F in hsca1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. Translation: AAA18300.1.
U05338 Genomic DNA. Translation: AAD13473.1.
U00096 Genomic DNA. Translation: AAC75579.1.
AP009048 Genomic DNA. Translation: BAA16420.1.
PIRiE65029.
RefSeqiNP_417021.1. NC_000913.3.
WP_001196613.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75579; AAC75579; b2526.
BAA16420; BAA16420; BAA16420.
GeneIDi944885.
KEGGiecj:JW2510.
eco:b2526.
PATRICi32120447. VBIEscCol129921_2626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. Translation: AAA18300.1.
U05338 Genomic DNA. Translation: AAD13473.1.
U00096 Genomic DNA. Translation: AAC75579.1.
AP009048 Genomic DNA. Translation: BAA16420.1.
PIRiE65029.
RefSeqiNP_417021.1. NC_000913.3.
WP_001196613.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U00X-ray1.95A389-615[»]
ProteinModelPortaliP0A6Z1.
SMRiP0A6Z1. Positions 23-615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262906. 73 interactions.
DIPiDIP-47348N.
IntActiP0A6Z1. 28 interactions.
STRINGi511145.b2526.

Proteomic databases

EPDiP0A6Z1.
PaxDbiP0A6Z1.
PRIDEiP0A6Z1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75579; AAC75579; b2526.
BAA16420; BAA16420; BAA16420.
GeneIDi944885.
KEGGiecj:JW2510.
eco:b2526.
PATRICi32120447. VBIEscCol129921_2626.

Organism-specific databases

EchoBASEiEB2051.
EcoGeneiEG12130. hscA.

Phylogenomic databases

eggNOGiENOG4105C9I. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
InParanoidiP0A6Z1.
KOiK04044.
OMAiLRGIPAM.
PhylomeDBiP0A6Z1.

Enzyme and pathway databases

BioCyciEcoCyc:EG12130-MONOMER.
ECOL316407:JW2510-MONOMER.
MetaCyc:EG12130-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6Z1.
PROiP0A6Z1.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00679. HscA. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
IPR010236. ISC_FeS_clus_asmbl_HscA.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR01991. HscA. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSCA_ECOLI
AccessioniPrimary (citable) accession number: P0A6Z1
Secondary accession number(s): P36541, P76990, P77497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.