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Reviewed, UniProtKB/Swiss-Prot P0A6Z1 (HSCA_ECOLI)

Last modified February 9, 2010. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Chaperone protein hscA
Alternative name(s):
    Hsc66
Gene names
Name: hscA
Synonyms: hsc
Ordered Locus Names: b2526, JW2510
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length616 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by hscB. Involved in the maturation of iscU. HAMAP MF_00679

Induction

By cold shock. Ref.6

Sequence similarities

Belongs to the heat shock protein 70 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 616616Chaperone protein hscA HAMAP MF_00679
PRO_0000078625

Natural variations

Natural variant2161S → F in hsca1.

Experimental info

Sequence conflict456 – 46510LRGIPALPAG → CVVFRRYRLA in AAA18300. Ref.1
Sequence conflict5161S → T in AAA18300. Ref.1
Sequence conflict5571Missing in AAA18300. Ref.1
Sequence conflict5831Q → K in AAD13473. Ref.2

Secondary structure

............................. 616
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A6Z1-1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 9C80E6EF1BFDAB48

FASTA61665,652
        10         20         30         40         50         60 
MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLADHEGR HLLPSVVHYQ 

        70         80         90        100        110        120 
QQGHSVGYDA RTNAALDTAN TISSVKRLMG RSLADIQQRY PHLPYQFQAS ENGLPMIETA 

       130        140        150        160        170        180 
AGLLNPVRVS ADILKALAAR ATEALAGELD GVVITVPAYF DDAQRQGTKD AARLAGLHVL 

       190        200        210        220        230        240 
RLLNEPTAAA IAYGLDSGQE GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD 

       250        260        270        280        290        300 
FDHLLADYIR EQAGIPDRSD NRVQRELLDA AIAAKIALSD ADSVTVNVAG WQGEISREQF 

       310        320        330        340        350        360 
NELIAPLVKR TLLACRRALK DAGVEADEVL EVVMVGGSTR VPLVRERVGE FFGRPPLTSI 

       370        380        390        400        410        420 
DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET MGGLVEKVIP RNTTIPVARA 

       430        440        450        460        470        480 
QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS LARFALRGIP ALPAGGAHIR VTFQVDADGL 

       490        500        510        520        530        540 
LSVTAMEKST GVEASIQVKP SYGLTDSEIA SMIKDSMSYA EQDVKARMLA EQKVEAARVL 

       550        560        570        580        590        600 
ESLHGALAAD AALLSAAERQ VIDDAAAHLS EVAQGDDVDA IEQAIKNVDK QTQDFAARRM 

       610 
DQSVRRALKG HSVDEV 

« Hide

References

« Hide 'large scale' references
[1]"Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and bgl activation, but not proU derepression, in hns-1 mutant Escherichia coli."
Kawula T.H., Lelivelt M.J.
J. Bacteriol. 176:610-619(1994) [PubMed: 8300516] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A gene encoding a DnaK/hsp70 homolog in Escherichia coli."
Seaton B.L., Vickery L.E.
Proc. Natl. Acad. Sci. U.S.A. 91:2066-2070(1994) [PubMed: 8134349] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B.
[3]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Hsc66, an Hsp70 homolog in Escherichia coli, is induced by cold shock but not by heat shock."
Lelivelt M.J., Kawula T.H.
J. Bacteriol. 177:4900-4907(1995) [PubMed: 7665466] [Abstract]
Cited for: INDUCTION.
[7]"Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli."
Vickery L.E., Silberg J.J., Ta D.T.
Protein Sci. 6:1047-1056(1997) [PubMed: 9144776] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli."
Hoff K.G., Silberg J.J., Vickery L.E.
Proc. Natl. Acad. Sci. U.S.A. 97:7790-7795(2000) [PubMed: 10869428] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01827 Unassigned DNA. Translation: AAA18300.1.
U05338 Genomic DNA. Translation: AAD13473.1.
U00096 Genomic DNA. Translation: AAC75579.1.
AP009048 Genomic DNA. Translation: BAA16420.1.
PIRE65029.
RefSeqAP_003112.1.
NP_417021.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U00X-ray1.95A390-615[»]
SMRP0A6Z1. Positions 21-581.
ModBaseSearch...

Protein-protein interaction databases

STRINGP0A6Z1.

Genome annotation databases

GeneID944885.
GenomeReviewsGene locus JW2510 in contig AP009048_GR.
Gene locus b2526 in contig U00096_GR.
KEGGecj:JW2510.
eco:b2526.

Organism-specific databases

EchoBASEEB2051.
EcoGeneEG12130. hscA.
CMRSearch...

Phylogenomic databases

eggNOGCOG0443.
HOGENOMHBG334976.
OMAKSTGVQS.

Enzyme and pathway databases

BioCycEcoCyc:EG12130-MONOMER.
ECOL168927:B2526-MONOMER.
MetaCyc:EG12130-MONOMER.

Gene expression databases

GenevestigatorP0A6Z1.

Family and domain databases

HAMAPMF_00679. HscA.
[Tree]
InterProIPR018181. Heat_shock_70_CS.
IPR001023. Hsp70.
IPR013126. Hsp_70.
IPR010236. ISC_FeS_clus_asmbl_HscA.
[Graphical view]
PANTHERPTHR19375. Hsp70. 1 hit.
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
TIGRFAMsTIGR01991. HscA. 1 hit.
PROSITEPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHSCA_ECOLI
AccessionPrimary (citable) accession number: P0A6Z1
Secondary accession number(s): P36541, P76990, P77497
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents