Reviewed, UniProtKB/Swiss-Prot P0A6Z0 (DNAK_ECO57)

Names and origin

Protein names

Recommended name:
    Chaperone protein dnaK
Alternative name(s):
    Heat shock protein 70
    Heat shock 70 kDa protein
    HSP70

Gene names

Name:	dnaK
Ordered Locus Names:	Z0014, ECs0014

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts as a chaperone By similarity.
Induction	By stress conditions e.g. heat shock By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 638	637	Chaperone protein dnaK HAMAP MF_00332		PRO_0000078460
Amino acid modifications
	Modified residue	109	1	N6-acetyllysine By similarity
	Modified residue	199	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	245	1	N6-acetyllysine By similarity
	Modified residue	304	1	N6-acetyllysine By similarity
	Modified residue	421	1	N6-acetyllysine By similarity
	Modified residue	556	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A6Z0-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5A8589B21D7CD9C1	FASTA	638	69,115
	10 20 30 40 50 60 MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT 70 80 90 100 110 120 NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL 130 140 150 160 170 180 KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG 190 200 210 220 230 240 LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL 250 260 270 280 290 300 VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV 310 320 330 340 350 360 TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE 370 380 390 400 410 420 PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT 430 440 450 460 470 480 KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA 490 500 510 520 530 540 DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD 550 560 570 580 590 600 HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME 610 620 630 IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG54314.1. BA000007 Genomic DNA. Translation: BAB33437.1.
PIR	F85481. F90630.
RefSeq	NP_285706.1. NP_308041.1.
3D structure databases
SMR	P0A6Z0. Positions 3-383, 389-603.
ModBase	Search...
Proteomic databases
PRIDE	P0A6Z0.
Genome annotation databases
GeneID	913406. 956673.
GenomeReviews	Gene locus Z0014 in contig AE005174_GR. Gene locus ECs0014 in contig BA000007_GR.
KEGG	ece:Z0014. ecs:ECs0014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A6Z0.
OMA	VHSTRKM
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0014-MON.
Family and domain databases
HAMAP	MF_00332. [Tree]
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_ECO57

Accession

Primary (citable) accession number: P0A6Z0
Secondary accession number(s): P04475

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)