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Protein

Integration host factor subunit beta

Gene

ihfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the 2 subunits of integration host factor (IHF), a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.
Plays a crucial role in the lysogenic life cycle of bacteriophage lambda, as it is required not only in the recombination reaction, which inserts lambda DNA into the E.coli chromosome, but also for the synthesis of int and cI repressor, two phage proteins necessary for DNA insertion and repression, respectively. The synthesis of int and cI proteins is regulated indirectly by IHF via translational control of the lambda cII protein.
Has an essential role in conjugative DNA transfer (CDT), the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Conjugation, DNA recombination, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00348.
ECOL316407:JW0895-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Integration host factor subunit beta
Short name:
IHF-beta
Gene namesi
Name:ihfB
Synonyms:himD, hip
Ordered Locus Names:b0912, JW0895
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10441. ihfB.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: InterPro
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 441E → G, K or V: Altered DNA-binding specificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9494Integration host factor subunit betaPRO_0000105048Add
BLAST

Proteomic databases

EPDiP0A6Y1.
PaxDbiP0A6Y1.
PRIDEiP0A6Y1.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Part of the relaxosome, a complex composed of plasmid-encoded TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT).2 Publications

Protein-protein interaction databases

BioGridi4263061. 174 interactions.
DIPiDIP-41099N.
IntActiP0A6Y1. 34 interactions.
MINTiMINT-365163.
STRINGi511145.b0912.

Structurei

Secondary structure

1
94
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1311Combined sources
Beta strandi15 – 173Combined sources
Helixi19 – 3820Combined sources
Beta strandi43 – 453Combined sources
Turni46 – 483Combined sources
Beta strandi49 – 568Combined sources
Beta strandi59 – 624Combined sources
Turni64 – 663Combined sources
Beta strandi69 – 724Combined sources
Beta strandi75 – 828Combined sources
Helixi84 – 907Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IHFX-ray2.20B1-94[»]
1OUZX-ray2.41B1-94[»]
1OWFX-ray1.95B1-94[»]
1OWGX-ray2.10B1-94[»]
2HT0X-ray2.00B1-94[»]
2IIEX-ray2.41A2-94[»]
2IIFX-ray2.72A2-94[»]
ProteinModelPortaliP0A6Y1.
SMRiP0A6Y1. Positions 1-93.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6Y1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41080YA. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0A6Y1.
KOiK05788.
OMAiRDRVNIY.
PhylomeDBiP0A6Y1.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
HAMAPiMF_00381. IHF_beta. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
IPR005685. IHF_beta.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
TIGRFAMsiTIGR00988. hip. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSELIERL ATQQSHIPAK TVEDAVKEML EHMASTLAQG ERIEIRGFGS
60 70 80 90
FSLHYRAPRT GRNPKTGDKV ELEGKYVPHF KPGKELRDRA NIYG
Length:94
Mass (Da):10,651
Last modified:June 7, 2005 - v1
Checksum:i7558D89EF469EA26
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91R → S AA sequence (PubMed:9868784).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04864 Genomic DNA. Translation: CAA28557.1.
U00096 Genomic DNA. Translation: AAC73998.1.
AP009048 Genomic DNA. Translation: BAA35656.1.
PIRiG64830. IQECAB.
RefSeqiNP_415432.1. NC_000913.3.
WP_000167336.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73998; AAC73998; b0912.
BAA35656; BAA35656; BAA35656.
GeneIDi945533.
KEGGiecj:JW0895.
eco:b0912.
PATRICi32117037. VBIEscCol129921_0943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04864 Genomic DNA. Translation: CAA28557.1.
U00096 Genomic DNA. Translation: AAC73998.1.
AP009048 Genomic DNA. Translation: BAA35656.1.
PIRiG64830. IQECAB.
RefSeqiNP_415432.1. NC_000913.3.
WP_000167336.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IHFX-ray2.20B1-94[»]
1OUZX-ray2.41B1-94[»]
1OWFX-ray1.95B1-94[»]
1OWGX-ray2.10B1-94[»]
2HT0X-ray2.00B1-94[»]
2IIEX-ray2.41A2-94[»]
2IIFX-ray2.72A2-94[»]
ProteinModelPortaliP0A6Y1.
SMRiP0A6Y1. Positions 1-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263061. 174 interactions.
DIPiDIP-41099N.
IntActiP0A6Y1. 34 interactions.
MINTiMINT-365163.
STRINGi511145.b0912.

Proteomic databases

EPDiP0A6Y1.
PaxDbiP0A6Y1.
PRIDEiP0A6Y1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73998; AAC73998; b0912.
BAA35656; BAA35656; BAA35656.
GeneIDi945533.
KEGGiecj:JW0895.
eco:b0912.
PATRICi32117037. VBIEscCol129921_0943.

Organism-specific databases

EchoBASEiEB0436.
EcoGeneiEG10441. ihfB.

Phylogenomic databases

eggNOGiENOG41080YA. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0A6Y1.
KOiK05788.
OMAiRDRVNIY.
PhylomeDBiP0A6Y1.

Enzyme and pathway databases

BioCyciEcoCyc:PD00348.
ECOL316407:JW0895-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6Y1.
PROiP0A6Y1.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
HAMAPiMF_00381. IHF_beta. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
IPR005685. IHF_beta.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
TIGRFAMsiTIGR00988. hip. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIHFB_ECOLI
AccessioniPrimary (citable) accession number: P0A6Y1
Secondary accession number(s): P08756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.