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Protein

Integration host factor subunit alpha

Gene

ihfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the 2 subunits of integration host factor (IHF), a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control. Binds to hundreds of transcriptionally inactive, AT-rich DNA sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779).1 Publication
Plays a crucial role in the lysogenic life cycle of bacteriophage lambda, as it is required not only in the recombination reaction, which inserts lambda DNA into the E.coli chromosome, but also for the synthesis of int and cI repressor, two phage proteins necessary for DNA insertion and repression, respectively. The synthesis of int and cI proteins is regulated indirectly by IHF via translational control of the lambda cII protein.
Has an essential role in conjugative DNA transfer (CDT), the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processConjugation, DNA recombination, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00347

Names & Taxonomyi

Protein namesi
Recommended name:
Integration host factor subunit alpha
Short name:
IHF-alpha
Gene namesi
Name:ihfA
Synonyms:hid, himA
Ordered Locus Names:b1712, JW1702
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10440 ihfA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • nucleoid Source: UniProtKB-SubCell
  • protein-DNA complex Source: CollecTF

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65P → L: Alters DNA-binding specificity. 1 Publication1
Mutagenesisi66K → S: Alters DNA-binding specificity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001050052 – 99Integration host factor subunit alphaAdd BLAST98

Proteomic databases

EPDiP0A6X7
PaxDbiP0A6X7
PRIDEiP0A6X7

Expressioni

Gene expression databases

CollecTFiEXPREG_00000780

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Part of the relaxosome, a complex composed of plasmid-encoded TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT).2 Publications

Protein-protein interaction databases

BioGridi4260284, 205 interactors
ComplexPortaliCPX-1957 IHF complex
DIPiDIP-36031N
IntActiP0A6X7, 24 interactors
STRINGi316385.ECDH10B_1848

Structurei

Secondary structure

199
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 16Combined sources12
Helixi20 – 39Combined sources20
Beta strandi44 – 46Combined sources3
Turni47 – 49Combined sources3
Beta strandi50 – 57Combined sources8
Beta strandi60 – 63Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi70 – 73Combined sources4
Beta strandi76 – 83Combined sources8
Helixi85 – 92Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IHFX-ray2.20A1-99[»]
1OUZX-ray2.41A1-99[»]
1OWFX-ray1.95A1-99[»]
1OWGX-ray2.10A1-99[»]
2HT0X-ray2.00A1-99[»]
2IIEX-ray2.41A3-94[»]
2IIFX-ray2.72A3-94[»]
5J0Nelectron microscopy11.00I/K2-97[»]
ProteinModelPortaliP0A6X7
SMRiP0A6X7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6X7

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0776 LUCA
HOGENOMiHOG000043829
InParanoidiP0A6X7
KOiK04764
OMAiFDLRDKK
PhylomeDBiP0A6X7

Family and domain databases

CDDicd13835 IHF_A, 1 hit
Gene3Di4.10.520.10, 1 hit
HAMAPiMF_00380 IHF_alpha, 1 hit
InterProiView protein in InterPro
IPR000119 Hist_DNA-bd
IPR020816 Histone-like_DNA-bd_CS
IPR010992 IHF-like_DNA-bd_dom_sf
IPR005684 IHF_alpha
PANTHERiPTHR33175 PTHR33175, 1 hit
PfamiView protein in Pfam
PF00216 Bac_DNA_binding, 1 hit
PRINTSiPR01727 DNABINDINGHU
SMARTiView protein in SMART
SM00411 BHL, 1 hit
SUPFAMiSSF47729 SSF47729, 1 hit
TIGRFAMsiTIGR00987 himA, 1 hit
PROSITEiView protein in PROSITE
PS00045 HISTONE_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTKAEMSE YLFDKLGLSK RDAKELVELF FEEIRRALEN GEQVKLSGFG
60 70 80 90
NFDLRDKNQR PGRNPKTGED IPITARRVVT FRPGQKLKSR VENASPKDE
Length:99
Mass (Da):11,354
Last modified:April 1, 1988 - v1
Checksum:i278AC82BCD79D754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02844 Genomic DNA Translation: AAA51471.1
V00291 Genomic DNA Translation: CAA23566.1
U00096 Genomic DNA Translation: AAC74782.1
AP009048 Genomic DNA Translation: BAA15480.1
M14031 Genomic DNA Translation: AAA23525.1
PIRiC23099 IQECAA
RefSeqiNP_416227.1, NC_000913.3
WP_001229265.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74782; AAC74782; b1712
BAA15480; BAA15480; BAA15480
GeneIDi945472
KEGGiecj:JW1702
eco:b1712
PATRICifig|1411691.4.peg.545

Similar proteinsi

Entry informationi

Entry nameiIHFA_ECOLI
AccessioniPrimary (citable) accession number: P0A6X7
Secondary accession number(s): P06984
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 20, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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