##gff-version 3
P0A6X1	UniProtKB	Chain	1	418	.	.	.	ID=PRO_0000114023;Note=Glutamyl-tRNA reductase	
P0A6X1	UniProtKB	Active site	50	50	.	.	.	Note=Nucleophile	
P0A6X1	UniProtKB	Binding site	49	52	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Binding site	109	109	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Binding site	114	116	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Binding site	120	120	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0A6X1	UniProtKB	Binding site	189	194	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Site	99	99	.	.	.	Note=Important for activity	
P0A6X1	UniProtKB	Mutagenesis	7	7	.	.	.	Note=Loss of activity. G->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	49	49	.	.	.	Note=10%25 and 5%25 of wild-type reductase and esterase activity%2C respectively. T->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	50	50	.	.	.	Note=Loss of activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	52	52	.	.	.	Note=5%25 and 4%25 of wild-type reductase and esterase activity%2C respectively. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	52	52	.	.	.	Note=Loss of activity. R->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	54	54	.	.	.	Note=6%25 and 2%25 of wild-type reductase and esterase activity%2C respectively. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	74	74	.	.	.	Note=No effect. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	99	99	.	.	.	Note=5%25 and 4%25 of wild-type reductase and esterase activity%2C respectively. H->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	106	106	.	.	.	Note=Loss of activity. G->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	109	109	.	.	.	Note=28%25 and 25%25 of wild-type reductase and esterase activity%2C respectively. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	114	114	.	.	.	Note=Loss of activity. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	116	116	.	.	.	Note=Loss of reductase activity. 30%25 of wild-type esterase activity. Q->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17697121;Dbxref=PMID:17697121	
P0A6X1	UniProtKB	Mutagenesis	145	145	.	.	.	Note=Loss of activity. S->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	170	170	.	.	.	Note=No effect. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	191	191	.	.	.	Note=Loss of reductase activity. Retains esterase activity. G->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Mutagenesis	314	314	.	.	.	Note=Loss of activity. R->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12370189;Dbxref=PMID:12370189	
P0A6X1	UniProtKB	Sequence conflict	151	168	.	.	.	Note=RVRTETDIGASAVSVAFA->PFALKQISVPALCLSLLP;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Sequence conflict	172	172	.	.	.	Note=L->V;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Sequence conflict	240	240	.	.	.	Note=L->M;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Sequence conflict	243	243	.	.	.	Note=A->R;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0A6X1	UniProtKB	Sequence conflict	365	365	.	.	.	Note=A->R;Ontology_term=ECO:0000305;evidence=ECO:0000305