P0A6X0 (GSH1_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	c3245

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP-Rule MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP-Rule MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Glutamate--cysteine ligase HAMAP-Rule MF_00578		PRO_0000192525

Sequences

	Sequence		Length	Mass (Da)
	P0A6X0 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 4E58C447B4D7FF16	FASTA	518	58,269
	10 20 30 40 50 60 MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS ALTHKWITTD 70 80 90 100 110 120 FAEALLEFIT PVDGDIEHML TFMRDLHRYT ARNMGDERMW PLSMPCYIAE GQDIELAQYG 130 140 150 160 170 180 TSNTGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGDISGA DAKEKISAGY 190 200 210 220 230 240 FRVIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTSLPFEK TECGMYYLPY ATSLRLSDLG 250 260 270 280 290 300 YTNKSQSNLG ITFNDLYEYV AGLKQAIKTP SEEYAKIGIE KDGKRLQINS NVLQIENELY 310 320 330 340 350 360 APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL DLFMVWCALA 370 380 390 400 410 420 DAPEMSSSEL ACTRVNWNRV ILEGRKPGLT LGIGCETAQF PLPQVGKDLF RDLKRVAQTL 430 440 450 460 470 480 DSINGGEAYQ KVCDELVACF DNPDLTFSAR ILRSMIDTGI GGTGKAFAEA YRNLLREEPL 490 500 510 EILREEDFVA EREASERRQQ EMEAADTEPF AVWLEKHA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN81697.1.
RefSeq	NP_755127.1. NC_004431.1.
3D structure databases
ProteinModelPortal	P0A6X0.
SMR	P0A6X0. Positions 1-518.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1302356.
STRING	199310.c3245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN81697; AAN81697; c3245.
GeneID	1039015.
KEGG	ecc:c3245.
PATRIC	18284319. VBIEscCol75197_3055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000266224.
KO	K01919.
OMA	EYIEVRA.
ProtClustDB	PRK02107.
Enzyme and pathway databases
SABIO-RK	P0A6X0.
UniPathway	UPA00142; UER00209.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase.
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_ECOL6

Accession

Primary (citable) accession number: P0A6X0
Secondary accession number(s): P06980, P78228

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program