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Protein

Glutamate--cysteine ligase

Gene

gshA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-cysteine ligase activity Source: EcoCyc
  • metal ion binding Source: EcoCyc

GO - Biological processi

  • cellular response to arsenic-containing substance Source: EcoCyc
  • cellular response to mercury ion Source: EcoCyc
  • glutathione biosynthetic process Source: EcoCyc
  • hyperosmotic response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLUTCYSLIG-MONOMER.
ECOL316407:JW2663-MONOMER.
MetaCyc:GLUTCYSLIG-MONOMER.
BRENDAi6.3.2.2. 2026.
SABIO-RKP0A6W9.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:gshA
Synonyms:gsh-I
Ordered Locus Names:b2688, JW2663
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10418. gshA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Glutamate--cysteine ligasePRO_0000192524Add
BLAST

Proteomic databases

EPDiP0A6W9.
PaxDbiP0A6W9.
PRIDEiP0A6W9.

Interactioni

Protein-protein interaction databases

BioGridi4262271. 8 interactions.
DIPiDIP-48212N.
IntActiP0A6W9. 5 interactions.
STRINGi511145.b2688.

Structurei

Secondary structure

1
518
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 149Combined sources
Helixi16 – 194Combined sources
Beta strandi23 – 3311Combined sources
Beta strandi37 – 393Combined sources
Helixi46 – 483Combined sources
Turni51 – 533Combined sources
Beta strandi55 – 595Combined sources
Beta strandi65 – 695Combined sources
Helixi76 – 9116Combined sources
Beta strandi102 – 1043Combined sources
Helixi111 – 1133Combined sources
Helixi123 – 13917Combined sources
Helixi141 – 1444Combined sources
Beta strandi148 – 1547Combined sources
Helixi157 – 1648Combined sources
Helixi169 – 19022Combined sources
Helixi193 – 1986Combined sources
Beta strandi201 – 2044Combined sources
Helixi206 – 2083Combined sources
Beta strandi224 – 2274Combined sources
Helixi234 – 2363Combined sources
Turni238 – 2403Combined sources
Helixi244 – 2463Combined sources
Helixi256 – 26712Combined sources
Helixi272 – 2754Combined sources
Beta strandi279 – 2813Combined sources
Beta strandi290 – 2923Combined sources
Helixi296 – 2983Combined sources
Beta strandi302 – 3065Combined sources
Helixi315 – 3228Combined sources
Beta strandi326 – 3338Combined sources
Helixi344 – 35916Combined sources
Helixi367 – 3726Combined sources
Helixi374 – 38310Combined sources
Helixi402 – 42423Combined sources
Helixi428 – 43710Combined sources
Turni438 – 4414Combined sources
Helixi443 – 4453Combined sources
Helixi447 – 45711Combined sources
Helixi464 – 47613Combined sources
Beta strandi481 – 4833Combined sources
Helixi485 – 50521Combined sources
Helixi510 – 5145Combined sources
Turni515 – 5173Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V4GX-ray2.50A/B/C/D1-518[»]
1VA6X-ray2.10A/B1-518[»]
2D32X-ray2.40A/B/C/D1-518[»]
2D33X-ray2.60A/B/C/D1-518[»]
ProteinModelPortaliP0A6W9.
SMRiP0A6W9. Positions 1-518.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6W9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D9M. Bacteria.
COG2918. LUCA.
HOGENOMiHOG000266224.
InParanoidiP0A6W9.
KOiK01919.
OMAiHRYGRRM.
PhylomeDBiP0A6W9.

Family and domain databases

HAMAPiMF_00578. Glu_cys_ligase. 1 hit.
InterProiIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01434. glu_cys_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6W9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS
60 70 80 90 100
ALTHKWITTD FAEALLEFIT PVDGDIEHML TFMRDLHRYT ARNMGDERMW
110 120 130 140 150
PLSMPCYIAE GQDIELAQYG TSNTGRFKTL YREGLKNRYG ALMQTISGVH
160 170 180 190 200
YNFSLPMAFW QAKCGDISGA DAKEKISAGY FRVIRNYYRF GWVIPYLFGA
210 220 230 240 250
SPAICSSFLQ GKPTSLPFEK TECGMYYLPY ATSLRLSDLG YTNKSQSNLG
260 270 280 290 300
ITFNDLYEYV AGLKQAIKTP SEEYAKIGIE KDGKRLQINS NVLQIENELY
310 320 330 340 350
APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL
360 370 380 390 400
DLFMVWCALA DAPEMSSSEL ACTRVNWNRV ILEGRKPGLT LGIGCETAQF
410 420 430 440 450
PLPQVGKDLF RDLKRVAQTL DSINGGEAYQ KVCDELVACF DNPDLTFSAR
460 470 480 490 500
ILRSMIDTGI GGTGKAFAEA YRNLLREEPL EILREEDFVA EREASERRQQ
510
EMEAADTEPF AVWLEKHA
Length:518
Mass (Da):58,269
Last modified:March 29, 2005 - v1
Checksum:i4E58C447B4D7FF16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001W → L in CAA27583 (PubMed:2872655).Curated
Sequence conflicti494 – 4952AS → GF in CAA27583 (PubMed:2872655).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03954 Genomic DNA. Translation: CAA27583.1.
U00096 Genomic DNA. Translation: AAC75735.1.
AP009048 Genomic DNA. Translation: BAA16555.1.
PIRiA65049. SYECEC.
RefSeqiNP_417173.1. NC_000913.3.
WP_000611804.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75735; AAC75735; b2688.
BAA16555; BAA16555; BAA16555.
GeneIDi944881.
KEGGiecj:JW2663.
eco:b2688.
PATRICi32120770. VBIEscCol129921_2782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03954 Genomic DNA. Translation: CAA27583.1.
U00096 Genomic DNA. Translation: AAC75735.1.
AP009048 Genomic DNA. Translation: BAA16555.1.
PIRiA65049. SYECEC.
RefSeqiNP_417173.1. NC_000913.3.
WP_000611804.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V4GX-ray2.50A/B/C/D1-518[»]
1VA6X-ray2.10A/B1-518[»]
2D32X-ray2.40A/B/C/D1-518[»]
2D33X-ray2.60A/B/C/D1-518[»]
ProteinModelPortaliP0A6W9.
SMRiP0A6W9. Positions 1-518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262271. 8 interactions.
DIPiDIP-48212N.
IntActiP0A6W9. 5 interactions.
STRINGi511145.b2688.

Proteomic databases

EPDiP0A6W9.
PaxDbiP0A6W9.
PRIDEiP0A6W9.

Protocols and materials databases

DNASUi944881.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75735; AAC75735; b2688.
BAA16555; BAA16555; BAA16555.
GeneIDi944881.
KEGGiecj:JW2663.
eco:b2688.
PATRICi32120770. VBIEscCol129921_2782.

Organism-specific databases

EchoBASEiEB0413.
EcoGeneiEG10418. gshA.

Phylogenomic databases

eggNOGiENOG4105D9M. Bacteria.
COG2918. LUCA.
HOGENOMiHOG000266224.
InParanoidiP0A6W9.
KOiK01919.
OMAiHRYGRRM.
PhylomeDBiP0A6W9.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BioCyciEcoCyc:GLUTCYSLIG-MONOMER.
ECOL316407:JW2663-MONOMER.
MetaCyc:GLUTCYSLIG-MONOMER.
BRENDAi6.3.2.2. 2026.
SABIO-RKP0A6W9.

Miscellaneous databases

EvolutionaryTraceiP0A6W9.
PROiP0A6W9.

Family and domain databases

HAMAPiMF_00578. Glu_cys_ligase. 1 hit.
InterProiIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01434. glu_cys_ligase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSH1_ECOLI
AccessioniPrimary (citable) accession number: P0A6W9
Secondary accession number(s): P06980, P78228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.