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Protein

Transcription elongation factor GreA

Gene

greA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.

GO - Molecular functioni

  • bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
  • DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • DNA-templated transcription, elongation Source: EcoCyc
  • regulation of DNA-templated transcription, elongation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor GreA
Alternative name(s):
Transcript cleavage factor GreA
Gene namesi
Name:greA
Ordered Locus Names:b3181, JW3148
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10415. greA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001769211 – 158Transcription elongation factor GreAAdd BLAST158

Proteomic databases

EPDiP0A6W5.
PaxDbiP0A6W5.
PRIDEiP0A6W5.

2D gel databases

SWISS-2DPAGEP0A6W5.

Interactioni

GO - Molecular functioni

  • bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262064. 36 interactors.
DIPiDIP-35800N.
IntActiP0A6W5. 51 interactors.
STRINGi511145.b3181.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi8 – 23Combined sources16
Helixi25 – 37Combined sources13
Helixi42 – 44Combined sources3
Helixi46 – 71Combined sources26
Beta strandi72 – 75Combined sources4
Helixi77 – 79Combined sources3
Beta strandi91 – 96Combined sources6
Turni97 – 100Combined sources4
Beta strandi101 – 108Combined sources8
Turni110 – 112Combined sources3
Helixi115 – 117Combined sources3
Beta strandi119 – 124Combined sources6
Helixi125 – 130Combined sources6
Beta strandi150 – 156Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GRJX-ray2.20A1-158[»]
ProteinModelPortaliP0A6W5.
SMRiP0A6W5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6W5.

Family & Domainsi

Sequence similaritiesi

Belongs to the GreA/GreB family.Curated

Phylogenomic databases

eggNOGiENOG4108UKH. Bacteria.
COG0782. LUCA.
HOGENOMiHOG000241145.
InParanoidiP0A6W5.
KOiK03624.
OMAiHNEGRIA.
PhylomeDBiP0A6W5.

Family and domain databases

Gene3Di1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPiMF_00105. GreA_GreB. 1 hit.
InterProiIPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]
PfamiPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006092. GreA_GreB. 1 hit.
SUPFAMiSSF46557. SSF46557. 1 hit.
TIGRFAMsiTIGR01462. greA. 1 hit.
PROSITEiPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA
60 70 80 90 100
AREQQGFCEG RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD
110 120 130 140 150
EEQTYRIVGD DEADFKQNLI SVNSPIARGL IGKEEDDVVV IKTPGGEVEF

EVIKVEYL
Length:158
Mass (Da):17,641
Last modified:March 29, 2005 - v1
Checksum:i9E7DFAFE838AC340
GO

Sequence cautioni

The sequence AAA57982 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54718 Genomic DNA. Translation: CAA38521.1.
U01376 Genomic DNA. Translation: AAA97506.1.
U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2.
AP009048 Genomic DNA. Translation: BAE77225.1.
PIRiG65108.
RefSeqiNP_417648.4. NC_000913.3.
WP_001148001.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76213; AAC76213; b3181.
BAE77225; BAE77225; BAE77225.
GeneIDi947696.
KEGGiecj:JW3148.
eco:b3181.
PATRICi32121780. VBIEscCol129921_3274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54718 Genomic DNA. Translation: CAA38521.1.
U01376 Genomic DNA. Translation: AAA97506.1.
U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2.
AP009048 Genomic DNA. Translation: BAE77225.1.
PIRiG65108.
RefSeqiNP_417648.4. NC_000913.3.
WP_001148001.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GRJX-ray2.20A1-158[»]
ProteinModelPortaliP0A6W5.
SMRiP0A6W5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262064. 36 interactors.
DIPiDIP-35800N.
IntActiP0A6W5. 51 interactors.
STRINGi511145.b3181.

2D gel databases

SWISS-2DPAGEP0A6W5.

Proteomic databases

EPDiP0A6W5.
PaxDbiP0A6W5.
PRIDEiP0A6W5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76213; AAC76213; b3181.
BAE77225; BAE77225; BAE77225.
GeneIDi947696.
KEGGiecj:JW3148.
eco:b3181.
PATRICi32121780. VBIEscCol129921_3274.

Organism-specific databases

EchoBASEiEB0410.
EcoGeneiEG10415. greA.

Phylogenomic databases

eggNOGiENOG4108UKH. Bacteria.
COG0782. LUCA.
HOGENOMiHOG000241145.
InParanoidiP0A6W5.
KOiK03624.
OMAiHNEGRIA.
PhylomeDBiP0A6W5.

Enzyme and pathway databases

BioCyciEcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6W5.
PROiP0A6W5.

Family and domain databases

Gene3Di1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPiMF_00105. GreA_GreB. 1 hit.
InterProiIPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]
PfamiPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006092. GreA_GreB. 1 hit.
SUPFAMiSSF46557. SSF46557. 1 hit.
TIGRFAMsiTIGR01462. greA. 1 hit.
PROSITEiPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGREA_ECOLI
AccessioniPrimary (citable) accession number: P0A6W5
Secondary accession number(s): P21346
, P78111, Q2M931, Q8X9K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.