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Protein

Transcription elongation factor GreA

Gene

greA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, elongation Source: EcoCyc
  • regulation of DNA-templated transcription, elongation Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10415-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor GreA
Alternative name(s):
Transcript cleavage factor GreA
Gene namesi
Name:greA
Ordered Locus Names:b3181, JW3148
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10415 greA

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001769211 – 158Transcription elongation factor GreAAdd BLAST158

Proteomic databases

EPDiP0A6W5
PaxDbiP0A6W5
PRIDEiP0A6W5

2D gel databases

SWISS-2DPAGEiP0A6W5

Interactioni

GO - Molecular functioni

  • bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
  • RNA polymerase binding Source: InterPro

Protein-protein interaction databases

BioGridi4262064, 36 interactors
DIPiDIP-35800N
IntActiP0A6W5, 52 interactors
STRINGi316385.ECDH10B_3355

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi8 – 23Combined sources16
Helixi25 – 37Combined sources13
Helixi42 – 44Combined sources3
Helixi46 – 71Combined sources26
Beta strandi72 – 75Combined sources4
Helixi77 – 79Combined sources3
Beta strandi91 – 96Combined sources6
Turni97 – 100Combined sources4
Beta strandi101 – 108Combined sources8
Turni110 – 112Combined sources3
Helixi115 – 117Combined sources3
Beta strandi119 – 124Combined sources6
Helixi125 – 130Combined sources6
Beta strandi150 – 156Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GRJX-ray2.20A1-158[»]
ProteinModelPortaliP0A6W5
SMRiP0A6W5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6W5

Family & Domainsi

Sequence similaritiesi

Belongs to the GreA/GreB family.Curated

Phylogenomic databases

eggNOGiENOG4108UKH Bacteria
COG0782 LUCA
HOGENOMiHOG000241145
InParanoidiP0A6W5
KOiK03624
OMAiTWLTQEA
PhylomeDBiP0A6W5

Family and domain databases

Gene3Di1.10.287.180, 1 hit
3.10.50.30, 1 hit
HAMAPiMF_00105 GreA_GreB, 1 hit
InterProiView protein in InterPro
IPR036953 GreA/GreB_C_sf
IPR018151 TF_GreA/GreB_CS
IPR006359 Tscrpt_elong_fac_GreA
IPR028624 Tscrpt_elong_fac_GreA/B
IPR001437 Tscrpt_elong_fac_GreA/B_C
IPR023459 Tscrpt_elong_fac_GreA/B_fam
IPR022691 Tscrpt_elong_fac_GreA/B_N
IPR036805 Tscrpt_elong_fac_GreA/B_N_sf
PANTHERiPTHR30437 PTHR30437, 1 hit
PfamiView protein in Pfam
PF01272 GreA_GreB, 1 hit
PF03449 GreA_GreB_N, 1 hit
PIRSFiPIRSF006092 GreA_GreB, 1 hit
SUPFAMiSSF46557 SSF46557, 1 hit
TIGRFAMsiTIGR01462 greA, 1 hit
PROSITEiView protein in PROSITE
PS00829 GREAB_1, 1 hit
PS00830 GREAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA
60 70 80 90 100
AREQQGFCEG RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD
110 120 130 140 150
EEQTYRIVGD DEADFKQNLI SVNSPIARGL IGKEEDDVVV IKTPGGEVEF

EVIKVEYL
Length:158
Mass (Da):17,641
Last modified:March 29, 2005 - v1
Checksum:i9E7DFAFE838AC340
GO

Sequence cautioni

The sequence AAA57982 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54718 Genomic DNA Translation: CAA38521.1
U01376 Genomic DNA Translation: AAA97506.1
U18997 Genomic DNA Translation: AAA57982.1 Different initiation.
U00096 Genomic DNA Translation: AAC76213.2
AP009048 Genomic DNA Translation: BAE77225.1
PIRiG65108
RefSeqiNP_417648.4, NC_000913.3
WP_001148001.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76213; AAC76213; b3181
BAE77225; BAE77225; BAE77225
GeneIDi947696
KEGGiecj:JW3148
eco:b3181
PATRICifig|1411691.4.peg.3551

Similar proteinsi

Entry informationi

Entry nameiGREA_ECOLI
AccessioniPrimary (citable) accession number: P0A6W5
Secondary accession number(s): P21346
, P78111, Q2M931, Q8X9K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health