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P0A6W5

- GREA_ECOLI

UniProt

P0A6W5 - GREA_ECOLI

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Protein
Transcription elongation factor GreA
Gene
greA, b3181, JW3148
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc

GO - Biological processi

  1. DNA-templated transcription, elongation Source: EcoCyc
  2. regulation of DNA-templated transcription, elongation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor GreA
Alternative name(s):
Transcript cleavage factor GreA
Gene namesi
Name:greA
Ordered Locus Names:b3181, JW3148
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10415. greA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Transcription elongation factor GreAUniRule annotation
PRO_0000176921Add
BLAST

Proteomic databases

PaxDbiP0A6W5.
PRIDEiP0A6W5.

2D gel databases

SWISS-2DPAGEP0A6W5.

Expressioni

Gene expression databases

GenevestigatoriP0A6W5.

Interactioni

Protein-protein interaction databases

DIPiDIP-35800N.
IntActiP0A6W5. 51 interactions.
STRINGi511145.b3181.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Helixi8 – 2316
Helixi25 – 3713
Helixi42 – 443
Helixi46 – 7126
Beta strandi72 – 754
Helixi77 – 793
Beta strandi91 – 966
Turni97 – 1004
Beta strandi101 – 1088
Turni110 – 1123
Helixi115 – 1173
Beta strandi119 – 1246
Helixi125 – 1306
Beta strandi150 – 1567

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GRJX-ray2.20A1-158[»]
ProteinModelPortaliP0A6W5.
SMRiP0A6W5. Positions 2-137.

Miscellaneous databases

EvolutionaryTraceiP0A6W5.

Family & Domainsi

Sequence similaritiesi

Belongs to the GreA/GreB family.

Phylogenomic databases

eggNOGiCOG0782.
HOGENOMiHOG000241145.
KOiK03624.
OMAiHAQIIDV.
OrthoDBiEOG686NQ9.
PhylomeDBiP0A6W5.

Family and domain databases

Gene3Di1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPiMF_00105. GreA_GreB.
InterProiIPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]
PfamiPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006092. GreA_GreB. 1 hit.
SUPFAMiSSF46557. SSF46557. 1 hit.
TIGRFAMsiTIGR01462. greA. 1 hit.
PROSITEiPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6W5-1 [UniParc]FASTAAdd to Basket

« Hide

MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA    50
AREQQGFCEG RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD 100
EEQTYRIVGD DEADFKQNLI SVNSPIARGL IGKEEDDVVV IKTPGGEVEF 150
EVIKVEYL 158
Length:158
Mass (Da):17,641
Last modified:March 29, 2005 - v1
Checksum:i9E7DFAFE838AC340
GO

Sequence cautioni

The sequence AAA57982.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54718 Genomic DNA. Translation: CAA38521.1.
U01376 Genomic DNA. Translation: AAA97506.1.
U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2.
AP009048 Genomic DNA. Translation: BAE77225.1.
PIRiG65108.
RefSeqiNP_417648.4. NC_000913.3.
YP_491366.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76213; AAC76213; b3181.
BAE77225; BAE77225; BAE77225.
GeneIDi12933070.
947696.
KEGGiecj:Y75_p3101.
eco:b3181.
PATRICi32121780. VBIEscCol129921_3274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54718 Genomic DNA. Translation: CAA38521.1 .
U01376 Genomic DNA. Translation: AAA97506.1 .
U18997 Genomic DNA. Translation: AAA57982.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2 .
AP009048 Genomic DNA. Translation: BAE77225.1 .
PIRi G65108.
RefSeqi NP_417648.4. NC_000913.3.
YP_491366.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GRJ X-ray 2.20 A 1-158 [» ]
ProteinModelPortali P0A6W5.
SMRi P0A6W5. Positions 2-137.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35800N.
IntActi P0A6W5. 51 interactions.
STRINGi 511145.b3181.

2D gel databases

SWISS-2DPAGE P0A6W5.

Proteomic databases

PaxDbi P0A6W5.
PRIDEi P0A6W5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76213 ; AAC76213 ; b3181 .
BAE77225 ; BAE77225 ; BAE77225 .
GeneIDi 12933070.
947696.
KEGGi ecj:Y75_p3101.
eco:b3181.
PATRICi 32121780. VBIEscCol129921_3274.

Organism-specific databases

EchoBASEi EB0410.
EcoGenei EG10415. greA.

Phylogenomic databases

eggNOGi COG0782.
HOGENOMi HOG000241145.
KOi K03624.
OMAi HAQIIDV.
OrthoDBi EOG686NQ9.
PhylomeDBi P0A6W5.

Enzyme and pathway databases

BioCyci EcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A6W5.
PROi P0A6W5.

Gene expression databases

Genevestigatori P0A6W5.

Family and domain databases

Gene3Di 1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPi MF_00105. GreA_GreB.
InterProi IPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view ]
Pfami PF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF006092. GreA_GreB. 1 hit.
SUPFAMi SSF46557. SSF46557. 1 hit.
TIGRFAMsi TIGR01462. greA. 1 hit.
PROSITEi PS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature."
    Sparkowski J., Das A.
    Nucleic Acids Res. 18:6443-6443(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Wang R., Kushner S.R.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  6. "Crystal structure of the GreA transcript cleavage factor from Escherichia coli."
    Stebbins C.E., Borukhov S., Orlova M., Polyakov A., Goldfarb A., Darst S.A.
    Nature 373:636-640(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiGREA_ECOLI
AccessioniPrimary (citable) accession number: P0A6W5
Secondary accession number(s): P21346
, P78111, Q2M931, Q8X9K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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