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P0A6W5 (GREA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription elongation factor GreA
Alternative name(s):
Transcript cleavage factor GreA
Gene names
Name:greA
Ordered Locus Names:b3181, JW3148
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. HAMAP-Rule MF_00105

Sequence similarities

Belongs to the GreA/GreB family.

Sequence caution

The sequence AAA57982.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Transcription elongation factor GreA HAMAP-Rule MF_00105
PRO_0000176921

Secondary structure

.......................... 158
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A6W5 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: 9E7DFAFE838AC340

FASTA15817,641
        10         20         30         40         50         60 
MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA AREQQGFCEG 

        70         80         90        100        110        120 
RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD EEQTYRIVGD DEADFKQNLI 

       130        140        150 
SVNSPIARGL IGKEEDDVVV IKTPGGEVEF EVIKVEYL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature."
Sparkowski J., Das A.
Nucleic Acids Res. 18:6443-6443(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Wang R., Kushner S.R.
Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[6]"Crystal structure of the GreA transcript cleavage factor from Escherichia coli."
Stebbins C.E., Borukhov S., Orlova M., Polyakov A., Goldfarb A., Darst S.A.
Nature 373:636-640(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54718 Genomic DNA. Translation: CAA38521.1.
U01376 Genomic DNA. Translation: AAA97506.1.
U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2.
AP009048 Genomic DNA. Translation: BAE77225.1.
PIRG65108.
RefSeqNP_417648.4. NC_000913.3.
YP_491366.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GRJX-ray2.20A1-158[»]
ProteinModelPortalP0A6W5.
SMRP0A6W5. Positions 2-137.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35800N.
IntActP0A6W5. 51 interactions.
STRING511145.b3181.

2D gel databases

SWISS-2DPAGEP0A6W5.

Proteomic databases

PaxDbP0A6W5.
PRIDEP0A6W5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76213; AAC76213; b3181.
BAE77225; BAE77225; BAE77225.
GeneID12933070.
947696.
KEGGecj:Y75_p3101.
eco:b3181.
PATRIC32121780. VBIEscCol129921_3274.

Organism-specific databases

EchoBASEEB0410.
EcoGeneEG10415. greA.

Phylogenomic databases

eggNOGCOG0782.
HOGENOMHOG000241145.
KOK03624.
OMAHNEGRIA.
OrthoDBEOG686NQ9.
PhylomeDBP0A6W5.
ProtClustDBPRK00226.

Enzyme and pathway databases

BioCycEcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Gene expression databases

GenevestigatorP0A6W5.

Family and domain databases

Gene3D1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPMF_00105. GreA_GreB.
InterProIPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]
PfamPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFPIRSF006092. GreA_GreB. 1 hit.
SUPFAMSSF46557. SSF46557. 1 hit.
TIGRFAMsTIGR01462. greA. 1 hit.
PROSITEPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A6W5.
PROP0A6W5.

Entry information

Entry nameGREA_ECOLI
AccessionPrimary (citable) accession number: P0A6W5
Secondary accession number(s): P21346 expand/collapse secondary AC list , P78111, Q2M931, Q8X9K9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene