P0A6W5 (GREA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor GreA

Alternative name(s):
Transcript cleavage factor GreA

Gene names

Name:	greA
Ordered Locus Names:	b3181, JW3148

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. HAMAP-Rule MF_00105
Sequence similarities	Belongs to the GreA/GreB family.
Sequence caution	The sequence AAA57982.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, elongation Inferred from direct assay PubMed 1384037. Source: EcoCyc regulation of DNA-dependent transcription, elongation Inferred from direct assay PubMed 1384037. Source: EcoCyc
Cellular_component	cytoplasm Inferred from direct assay PubMed 16858726. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP bacterial-type RNA polymerase holo enzyme binding Inferred from direct assay PubMed 10625443. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Transcription elongation factor GreA HAMAP-Rule MF_00105

PRO_0000176921

Secondary structure

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158

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Helix

8 – 23

16

Helix

25 – 37

13

Helix

42 – 44

3

Helix

46 – 71

26

Beta strand

72 – 75

4

Helix

77 – 79

3

Beta strand

91 – 96

6

Turn

97 – 100

4

Beta strand

101 – 108

8

Turn

110 – 112

3

Helix

115 – 117

3

Beta strand

119 – 124

6

Helix

125 – 130

6

Beta strand

150 – 156

7

Sequences

	Sequence		Length	Mass (Da)
	P0A6W5 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 9E7DFAFE838AC340	FASTA	158	17,641
	10 20 30 40 50 60 MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA AREQQGFCEG 70 80 90 100 110 120 RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD EEQTYRIVGD DEADFKQNLI 130 140 150 SVNSPIARGL IGKEEDDVVV IKTPGGEVEF EVIKVEYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature." Sparkowski J., Das A. Nucleic Acids Res. 18:6443-6443(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Wang R., Kushner S.R. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[6]	"Crystal structure of the GreA transcript cleavage factor from Escherichia coli." Stebbins C.E., Borukhov S., Orlova M., Polyakov A., Goldfarb A., Darst S.A. Nature 373:636-640(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X54718 Genomic DNA. Translation: CAA38521.1.
U01376 Genomic DNA. Translation: AAA97506.1.
U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76213.2.
AP009048 Genomic DNA. Translation: BAE77225.1.

PIR

G65108.

RefSeq

NP_417648.4. NC_000913.2.
YP_491366.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GRJ	X-ray	2.20	A	1-158	[»]

ProteinModelPortal

P0A6W5.

SMR

P0A6W5. Positions 2-137.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35800N.

IntAct

P0A6W5. 51 interactions.

STRING

511145.b3181.

2D gel databases

SWISS-2DPAGE

P0A6W5.

Proteomic databases

PaxDb

P0A6W5.

PRIDE

P0A6W5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76213; AAC76213; b3181.
BAE77225; BAE77225; BAE77225.

GeneID

12933070.
947696.

KEGG

ecj:Y75_p3101.
eco:b3181.

PATRIC

32121780. VBIEscCol129921_3274.

Organism-specific databases

EchoBASE

EB0410.

EcoGene

EG10415. greA.

Phylogenomic databases

eggNOG

COG0782.

HOGENOM

HOG000241145.

KO

K03624.

OMA

HNEGRIA.

ProtClustDB

PRK00226.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10415-MONOMER.
ECOL316407:JW3148-MONOMER.

Gene expression databases

Genevestigator

P0A6W5.

Family and domain databases

Gene3D

1.10.287.180. 1 hit.
3.10.50.30. 1 hit.

HAMAP

MF_00105. GreA_GreB.

InterPro

IPR018151. TF_GreA/GreB_CS.
IPR006359. Tscrpt_elong_fac_GreA.
IPR023459. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]

Pfam

PF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]

PIRSF

PIRSF006092. GreA_GreB. 1 hit.

SUPFAM

SSF46557. GreA_GreB. 1 hit.

TIGRFAMs

TIGR01462. greA. 1 hit.

PROSITE

PS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A6W5.

Entry information

Entry name

GREA_ECOLI

Accession

Primary (citable) accession number: P0A6W5
Secondary accession number(s): P21346 , P78111, Q2M931, Q8X9K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program