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P0A6W5

- GREA_ECOLI

UniProt

P0A6W5 - GREA_ECOLI

Protein

Transcription elongation factor GreA

Gene

greA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.

    GO - Molecular functioni

    1. bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA-templated transcription, elongation Source: EcoCyc
    2. regulation of DNA-templated transcription, elongation Source: EcoCyc

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10415-MONOMER.
    ECOL316407:JW3148-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription elongation factor GreA
    Alternative name(s):
    Transcript cleavage factor GreA
    Gene namesi
    Name:greA
    Ordered Locus Names:b3181, JW3148
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10415. greA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Transcription elongation factor GreAPRO_0000176921Add
    BLAST

    Proteomic databases

    PaxDbiP0A6W5.
    PRIDEiP0A6W5.

    2D gel databases

    SWISS-2DPAGEP0A6W5.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6W5.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-35800N.
    IntActiP0A6W5. 51 interactions.
    STRINGi511145.b3181.

    Structurei

    Secondary structure

    1
    158
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Helixi8 – 2316
    Helixi25 – 3713
    Helixi42 – 443
    Helixi46 – 7126
    Beta strandi72 – 754
    Helixi77 – 793
    Beta strandi91 – 966
    Turni97 – 1004
    Beta strandi101 – 1088
    Turni110 – 1123
    Helixi115 – 1173
    Beta strandi119 – 1246
    Helixi125 – 1306
    Beta strandi150 – 1567

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GRJX-ray2.20A1-158[»]
    ProteinModelPortaliP0A6W5.
    SMRiP0A6W5. Positions 2-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A6W5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GreA/GreB family.Curated

    Phylogenomic databases

    eggNOGiCOG0782.
    HOGENOMiHOG000241145.
    KOiK03624.
    OMAiHAQIIDV.
    OrthoDBiEOG686NQ9.
    PhylomeDBiP0A6W5.

    Family and domain databases

    Gene3Di1.10.287.180. 1 hit.
    3.10.50.30. 1 hit.
    HAMAPiMF_00105. GreA_GreB.
    InterProiIPR018151. TF_GreA/GreB_CS.
    IPR006359. Tscrpt_elong_fac_GreA.
    IPR028624. Tscrpt_elong_fac_GreA/B.
    IPR001437. Tscrpt_elong_fac_GreA/B_C.
    IPR023459. Tscrpt_elong_fac_GreA/B_fam.
    IPR022691. Tscrpt_elong_fac_GreA/B_N.
    [Graphical view]
    PfamiPF01272. GreA_GreB. 1 hit.
    PF03449. GreA_GreB_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006092. GreA_GreB. 1 hit.
    SUPFAMiSSF46557. SSF46557. 1 hit.
    TIGRFAMsiTIGR01462. greA. 1 hit.
    PROSITEiPS00829. GREAB_1. 1 hit.
    PS00830. GREAB_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A6W5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA    50
    AREQQGFCEG RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD 100
    EEQTYRIVGD DEADFKQNLI SVNSPIARGL IGKEEDDVVV IKTPGGEVEF 150
    EVIKVEYL 158
    Length:158
    Mass (Da):17,641
    Last modified:March 29, 2005 - v1
    Checksum:i9E7DFAFE838AC340
    GO

    Sequence cautioni

    The sequence AAA57982.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54718 Genomic DNA. Translation: CAA38521.1.
    U01376 Genomic DNA. Translation: AAA97506.1.
    U18997 Genomic DNA. Translation: AAA57982.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76213.2.
    AP009048 Genomic DNA. Translation: BAE77225.1.
    PIRiG65108.
    RefSeqiNP_417648.4. NC_000913.3.
    YP_491366.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76213; AAC76213; b3181.
    BAE77225; BAE77225; BAE77225.
    GeneIDi12933070.
    947696.
    KEGGiecj:Y75_p3101.
    eco:b3181.
    PATRICi32121780. VBIEscCol129921_3274.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54718 Genomic DNA. Translation: CAA38521.1 .
    U01376 Genomic DNA. Translation: AAA97506.1 .
    U18997 Genomic DNA. Translation: AAA57982.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC76213.2 .
    AP009048 Genomic DNA. Translation: BAE77225.1 .
    PIRi G65108.
    RefSeqi NP_417648.4. NC_000913.3.
    YP_491366.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GRJ X-ray 2.20 A 1-158 [» ]
    ProteinModelPortali P0A6W5.
    SMRi P0A6W5. Positions 2-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35800N.
    IntActi P0A6W5. 51 interactions.
    STRINGi 511145.b3181.

    2D gel databases

    SWISS-2DPAGE P0A6W5.

    Proteomic databases

    PaxDbi P0A6W5.
    PRIDEi P0A6W5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76213 ; AAC76213 ; b3181 .
    BAE77225 ; BAE77225 ; BAE77225 .
    GeneIDi 12933070.
    947696.
    KEGGi ecj:Y75_p3101.
    eco:b3181.
    PATRICi 32121780. VBIEscCol129921_3274.

    Organism-specific databases

    EchoBASEi EB0410.
    EcoGenei EG10415. greA.

    Phylogenomic databases

    eggNOGi COG0782.
    HOGENOMi HOG000241145.
    KOi K03624.
    OMAi HAQIIDV.
    OrthoDBi EOG686NQ9.
    PhylomeDBi P0A6W5.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10415-MONOMER.
    ECOL316407:JW3148-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A6W5.
    PROi P0A6W5.

    Gene expression databases

    Genevestigatori P0A6W5.

    Family and domain databases

    Gene3Di 1.10.287.180. 1 hit.
    3.10.50.30. 1 hit.
    HAMAPi MF_00105. GreA_GreB.
    InterProi IPR018151. TF_GreA/GreB_CS.
    IPR006359. Tscrpt_elong_fac_GreA.
    IPR028624. Tscrpt_elong_fac_GreA/B.
    IPR001437. Tscrpt_elong_fac_GreA/B_C.
    IPR023459. Tscrpt_elong_fac_GreA/B_fam.
    IPR022691. Tscrpt_elong_fac_GreA/B_N.
    [Graphical view ]
    Pfami PF01272. GreA_GreB. 1 hit.
    PF03449. GreA_GreB_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006092. GreA_GreB. 1 hit.
    SUPFAMi SSF46557. SSF46557. 1 hit.
    TIGRFAMsi TIGR01462. greA. 1 hit.
    PROSITEi PS00829. GREAB_1. 1 hit.
    PS00830. GREAB_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature."
      Sparkowski J., Das A.
      Nucleic Acids Res. 18:6443-6443(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Wang R., Kushner S.R.
      Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    6. "Crystal structure of the GreA transcript cleavage factor from Escherichia coli."
      Stebbins C.E., Borukhov S., Orlova M., Polyakov A., Goldfarb A., Darst S.A.
      Nature 373:636-640(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiGREA_ECOLI
    AccessioniPrimary (citable) accession number: P0A6W5
    Secondary accession number(s): P21346
    , P78111, Q2M931, Q8X9K9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3