P0A6W0 (GLSA2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase 2
EC=3.5.1.2

Gene names

Name:	glsA2
Synonyms:	yneH
Ordered Locus Names:	b1524, JW1517

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. Ref.4
Subunit structure	Homotetramer. Ref.4
Sequence similarities	Belongs to the glutaminase family.
Biophysicochemical properties	Kinetic parameters: KM=30.6 mM for glutamine Ref.4

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine catabolic process Inferred from mutant phenotype Ref.4. Source: EcoCyc negative regulation of growth Inferred from mutant phenotype Ref.4. Source: EcoliWiki
Molecular_function	glutaminase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glutaminase 2 HAMAP-Rule MF_00313		PRO_0000110608
Sites
	Binding site	66	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	262	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A6W0 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: DDC39D9DC0D8A651	FASTA	308	33,516
	10 20 30 40 50 60 MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG QLFQAGDAQE 70 80 90 100 110 120 RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS LVQLEMEQGI PRNPFINAGA 130 140 150 160 170 180 LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD ISYDTVVARS EFEHSARNAA IAWLMKSFGN 190 200 210 220 230 240 FHHDVTTVLQ NYFHYCALKM SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT 250 260 270 280 290 300 SGMYQNAGEF AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT KQLGRSVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis." Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F. Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74597.1. AP009048 Genomic DNA. Translation: BAA15206.1.
PIR	G64906.
RefSeq	NP_416041.1. NC_000913.2. YP_489787.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A6W0.
SMR	P0A6W0. Positions 24-308.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A6W0. 3 interactions.
STRING	511145.b1524.
Proteomic databases
PRIDE	P0A6W0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74597; AAC74597; b1524. BAA15206; BAA15206; BAA15206.
GeneID	12932701. 944973.
KEGG	ecj:Y75_p1499. eco:b1524.
PATRIC	32118346. VBIEscCol129921_1593.
Organism-specific databases
EchoBASE	EB3577.
EcoGene	EG13816. glsA2.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	EcoCyc:G6810-MONOMER. ECOL316407:JW1517-MONOMER. MetaCyc:G6810-MONOMER.
Gene expression databases
Genevestigator	P0A6W0.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA2_ECOLI

Accession

Primary (citable) accession number: P0A6W0
Secondary accession number(s): P77470

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program