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Protein

Glutaminase 2

Gene

glsA2

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation1 Publication

Kineticsi

  1. KM=30.6 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei66SubstrateUniRule annotation1
    Binding sitei117SubstrateUniRule annotation1
    Binding sitei161SubstrateUniRule annotation1
    Binding sitei168SubstrateUniRule annotation1
    Binding sitei192SubstrateUniRule annotation1
    Binding sitei244SubstrateUniRule annotation1
    Binding sitei262Substrate; via amide nitrogenUniRule annotation1

    GO - Molecular functioni

    • glutaminase activity Source: EcoCyc

    GO - Biological processi

    • glutamate biosynthetic process Source: GO_Central
    • glutamine catabolic process Source: EcoCyc
    • negative regulation of growth Source: EcoliWiki

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6810-MONOMER
    MetaCyc:G6810-MONOMER
    BRENDAi3.5.1.2 2026

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsA2UniRule annotation
    Synonyms:yneH
    Ordered Locus Names:b1524, JW1517
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13816 glsA2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001106081 – 308Glutaminase 2Add BLAST308

    Proteomic databases

    PaxDbiP0A6W0
    PRIDEiP0A6W0

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Protein-protein interaction databases

    BioGridi4261695, 8 interactors
    DIPiDIP-12757N
    IntActiP0A6W0, 3 interactors
    STRINGi316385.ECDH10B_1655

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6W0
    SMRiP0A6W0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105CSV Bacteria
    COG2066 LUCA
    HOGENOMiHOG000216890
    InParanoidiP0A6W0
    KOiK01425
    OMAiEPSGNPF
    PhylomeDBiP0A6W0

    Family and domain databases

    HAMAPiMF_00313 Glutaminase, 1 hit
    InterProiView protein in InterPro
    IPR012338 Beta-lactam/transpept-like
    IPR015868 Glutaminase
    PANTHERiPTHR12544 PTHR12544, 1 hit
    PfamiView protein in Pfam
    PF04960 Glutaminase, 1 hit
    SUPFAMiSSF56601 SSF56601, 1 hit
    TIGRFAMsiTIGR03814 Gln_ase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A6W0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG
    60 70 80 90 100
    QLFQAGDAQE RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS
    110 120 130 140 150
    LVQLEMEQGI PRNPFINAGA LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD
    160 170 180 190 200
    ISYDTVVARS EFEHSARNAA IAWLMKSFGN FHHDVTTVLQ NYFHYCALKM
    210 220 230 240 250
    SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT SGMYQNAGEF
    260 270 280 290 300
    AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT

    KQLGRSVY
    Length:308
    Mass (Da):33,516
    Last modified:March 29, 2005 - v1
    Checksum:iDDC39D9DC0D8A651
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74597.1
    AP009048 Genomic DNA Translation: BAA15206.1
    PIRiG64906
    RefSeqiNP_416041.1, NC_000913.3
    WP_000257409.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74597; AAC74597; b1524
    BAA15206; BAA15206; BAA15206
    GeneIDi944973
    KEGGiecj:JW1517
    eco:b1524
    PATRICifig|1411691.4.peg.742

    Similar proteinsi

    Entry informationi

    Entry nameiGLSA2_ECOLI
    AccessioniPrimary (citable) accession number: P0A6W0
    Secondary accession number(s): P77470
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: March 28, 2018
    This is version 95 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health