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Protein

Glutaminase 2

Gene

glsA2

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation1 Publication

Kineticsi

  1. KM=30.6 mM for glutamine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661SubstrateUniRule annotation
Binding sitei117 – 1171SubstrateUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Binding sitei244 – 2441SubstrateUniRule annotation
Binding sitei262 – 2621Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. glutaminase activity Source: EcoCyc

GO - Biological processi

  1. glutamine catabolic process Source: EcoCyc
  2. negative regulation of growth Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6810-MONOMER.
ECOL316407:JW1517-MONOMER.
MetaCyc:G6810-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:glsA2UniRule annotation
Synonyms:yneH
Ordered Locus Names:b1524, JW1517
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13816. glsA2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Glutaminase 2PRO_0000110608Add
BLAST

Proteomic databases

PRIDEiP0A6W0.

Expressioni

Gene expression databases

GenevestigatoriP0A6W0.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

DIPiDIP-12757N.
IntActiP0A6W0. 3 interactions.
STRINGi511145.b1524.

Structurei

3D structure databases

ProteinModelPortaliP0A6W0.
SMRiP0A6W0. Positions 24-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
InParanoidiP0A6W0.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.
PhylomeDBiP0A6W0.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG
60 70 80 90 100
QLFQAGDAQE RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS
110 120 130 140 150
LVQLEMEQGI PRNPFINAGA LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD
160 170 180 190 200
ISYDTVVARS EFEHSARNAA IAWLMKSFGN FHHDVTTVLQ NYFHYCALKM
210 220 230 240 250
SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT SGMYQNAGEF
260 270 280 290 300
AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT

KQLGRSVY
Length:308
Mass (Da):33,516
Last modified:March 29, 2005 - v1
Checksum:iDDC39D9DC0D8A651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74597.1.
AP009048 Genomic DNA. Translation: BAA15206.1.
PIRiG64906.
RefSeqiNP_416041.1. NC_000913.3.
YP_489787.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74597; AAC74597; b1524.
BAA15206; BAA15206; BAA15206.
GeneIDi12932701.
944973.
KEGGiecj:Y75_p1499.
eco:b1524.
PATRICi32118346. VBIEscCol129921_1593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74597.1.
AP009048 Genomic DNA. Translation: BAA15206.1.
PIRiG64906.
RefSeqiNP_416041.1. NC_000913.3.
YP_489787.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0A6W0.
SMRiP0A6W0. Positions 24-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12757N.
IntActiP0A6W0. 3 interactions.
STRINGi511145.b1524.

Proteomic databases

PRIDEiP0A6W0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74597; AAC74597; b1524.
BAA15206; BAA15206; BAA15206.
GeneIDi12932701.
944973.
KEGGiecj:Y75_p1499.
eco:b1524.
PATRICi32118346. VBIEscCol129921_1593.

Organism-specific databases

EchoBASEiEB3577.
EcoGeneiEG13816. glsA2.

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
InParanoidiP0A6W0.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.
PhylomeDBiP0A6W0.

Enzyme and pathway databases

BioCyciEcoCyc:G6810-MONOMER.
ECOL316407:JW1517-MONOMER.
MetaCyc:G6810-MONOMER.

Miscellaneous databases

PROiP0A6W0.

Gene expression databases

GenevestigatoriP0A6W0.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
    Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
    Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiGLSA2_ECOLI
AccessioniPrimary (citable) accession number: P0A6W0
Secondary accession number(s): P77470
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.