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P0A6W0

- GLSA2_ECOLI

UniProt

P0A6W0 - GLSA2_ECOLI

Protein

Glutaminase 2

Gene

glsA2

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.1 PublicationUniRule annotation

    Kineticsi

    1. KM=30.6 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei66 – 661SubstrateUniRule annotation
    Binding sitei117 – 1171SubstrateUniRule annotation
    Binding sitei161 – 1611SubstrateUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Binding sitei192 – 1921SubstrateUniRule annotation
    Binding sitei244 – 2441SubstrateUniRule annotation
    Binding sitei262 – 2621Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. glutaminase activity Source: EcoCyc

    GO - Biological processi

    1. glutamine catabolic process Source: EcoCyc
    2. negative regulation of growth Source: EcoliWiki

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6810-MONOMER.
    ECOL316407:JW1517-MONOMER.
    MetaCyc:G6810-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsA2UniRule annotation
    Synonyms:yneH
    Ordered Locus Names:b1524, JW1517
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13816. glsA2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Glutaminase 2PRO_0000110608Add
    BLAST

    Proteomic databases

    PRIDEiP0A6W0.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6W0.

    Interactioni

    Subunit structurei

    Homotetramer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-12757N.
    IntActiP0A6W0. 3 interactions.
    STRINGi511145.b1524.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6W0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    KOiK01425.
    OMAiMSIAVWS.
    OrthoDBiEOG6N94BK.
    PhylomeDBiP0A6W0.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6W0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG    50
    QLFQAGDAQE RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS 100
    LVQLEMEQGI PRNPFINAGA LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD 150
    ISYDTVVARS EFEHSARNAA IAWLMKSFGN FHHDVTTVLQ NYFHYCALKM 200
    SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT SGMYQNAGEF 250
    AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT 300
    KQLGRSVY 308
    Length:308
    Mass (Da):33,516
    Last modified:March 29, 2005 - v1
    Checksum:iDDC39D9DC0D8A651
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74597.1.
    AP009048 Genomic DNA. Translation: BAA15206.1.
    PIRiG64906.
    RefSeqiNP_416041.1. NC_000913.3.
    YP_489787.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74597; AAC74597; b1524.
    BAA15206; BAA15206; BAA15206.
    GeneIDi12932701.
    944973.
    KEGGiecj:Y75_p1499.
    eco:b1524.
    PATRICi32118346. VBIEscCol129921_1593.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74597.1 .
    AP009048 Genomic DNA. Translation: BAA15206.1 .
    PIRi G64906.
    RefSeqi NP_416041.1. NC_000913.3.
    YP_489787.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A6W0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12757N.
    IntActi P0A6W0. 3 interactions.
    STRINGi 511145.b1524.

    Proteomic databases

    PRIDEi P0A6W0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74597 ; AAC74597 ; b1524 .
    BAA15206 ; BAA15206 ; BAA15206 .
    GeneIDi 12932701.
    944973.
    KEGGi ecj:Y75_p1499.
    eco:b1524.
    PATRICi 32118346. VBIEscCol129921_1593.

    Organism-specific databases

    EchoBASEi EB3577.
    EcoGenei EG13816. glsA2.

    Phylogenomic databases

    eggNOGi COG2066.
    HOGENOMi HOG000216890.
    KOi K01425.
    OMAi MSIAVWS.
    OrthoDBi EOG6N94BK.
    PhylomeDBi P0A6W0.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6810-MONOMER.
    ECOL316407:JW1517-MONOMER.
    MetaCyc:G6810-MONOMER.

    Miscellaneous databases

    PROi P0A6W0.

    Gene expression databases

    Genevestigatori P0A6W0.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
      Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
      Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiGLSA2_ECOLI
    AccessioniPrimary (citable) accession number: P0A6W0
    Secondary accession number(s): P77470
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3