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Protein

Glutaminase 2

Gene

glsA2

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation1 Publication

Kineticsi

  1. KM=30.6 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei66 – 661SubstrateUniRule annotation
    Binding sitei117 – 1171SubstrateUniRule annotation
    Binding sitei161 – 1611SubstrateUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Binding sitei192 – 1921SubstrateUniRule annotation
    Binding sitei244 – 2441SubstrateUniRule annotation
    Binding sitei262 – 2621Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    • glutaminase activity Source: EcoCyc

    GO - Biological processi

    • glutamine catabolic process Source: EcoCyc
    • negative regulation of growth Source: EcoliWiki
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6810-MONOMER.
    ECOL316407:JW1517-MONOMER.
    MetaCyc:G6810-MONOMER.
    BRENDAi3.5.1.2. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsA2UniRule annotation
    Synonyms:yneH
    Ordered Locus Names:b1524, JW1517
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13816. glsA2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Glutaminase 2PRO_0000110608Add
    BLAST

    Proteomic databases

    PRIDEiP0A6W0.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Protein-protein interaction databases

    DIPiDIP-12757N.
    IntActiP0A6W0. 3 interactions.
    STRINGi511145.b1524.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6W0.
    SMRiP0A6W0. Positions 24-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    InParanoidiP0A6W0.
    KOiK01425.
    OMAiVNSIMAT.
    OrthoDBiEOG6N94BK.
    PhylomeDBiP0A6W0.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6W0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG
    60 70 80 90 100
    QLFQAGDAQE RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS
    110 120 130 140 150
    LVQLEMEQGI PRNPFINAGA LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD
    160 170 180 190 200
    ISYDTVVARS EFEHSARNAA IAWLMKSFGN FHHDVTTVLQ NYFHYCALKM
    210 220 230 240 250
    SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT SGMYQNAGEF
    260 270 280 290 300
    AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT

    KQLGRSVY
    Length:308
    Mass (Da):33,516
    Last modified:March 29, 2005 - v1
    Checksum:iDDC39D9DC0D8A651
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74597.1.
    AP009048 Genomic DNA. Translation: BAA15206.1.
    PIRiG64906.
    RefSeqiNP_416041.1. NC_000913.3.
    WP_000257409.1. NZ_CP010445.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74597; AAC74597; b1524.
    BAA15206; BAA15206; BAA15206.
    GeneIDi944973.
    KEGGieco:b1524.
    PATRICi32118346. VBIEscCol129921_1593.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74597.1.
    AP009048 Genomic DNA. Translation: BAA15206.1.
    PIRiG64906.
    RefSeqiNP_416041.1. NC_000913.3.
    WP_000257409.1. NZ_CP010445.1.

    3D structure databases

    ProteinModelPortaliP0A6W0.
    SMRiP0A6W0. Positions 24-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-12757N.
    IntActiP0A6W0. 3 interactions.
    STRINGi511145.b1524.

    Proteomic databases

    PRIDEiP0A6W0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74597; AAC74597; b1524.
    BAA15206; BAA15206; BAA15206.
    GeneIDi944973.
    KEGGieco:b1524.
    PATRICi32118346. VBIEscCol129921_1593.

    Organism-specific databases

    EchoBASEiEB3577.
    EcoGeneiEG13816. glsA2.

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    InParanoidiP0A6W0.
    KOiK01425.
    OMAiVNSIMAT.
    OrthoDBiEOG6N94BK.
    PhylomeDBiP0A6W0.

    Enzyme and pathway databases

    BioCyciEcoCyc:G6810-MONOMER.
    ECOL316407:JW1517-MONOMER.
    MetaCyc:G6810-MONOMER.
    BRENDAi3.5.1.2. 2026.

    Miscellaneous databases

    PROiP0A6W0.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
      Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
      Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiGLSA2_ECOLI
    AccessioniPrimary (citable) accession number: P0A6W0
    Secondary accession number(s): P77470
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: July 22, 2015
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.