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P0A6W0

- GLSA2_ECOLI

UniProt

P0A6W0 - GLSA2_ECOLI

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Protein

Glutaminase 2

Gene

glsA2

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.1 PublicationUniRule annotation

Kineticsi

  1. KM=30.6 mM for glutamine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661SubstrateUniRule annotation
Binding sitei117 – 1171SubstrateUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Binding sitei244 – 2441SubstrateUniRule annotation
Binding sitei262 – 2621Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. glutaminase activity Source: EcoCyc

GO - Biological processi

  1. glutamine catabolic process Source: EcoCyc
  2. negative regulation of growth Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6810-MONOMER.
ECOL316407:JW1517-MONOMER.
MetaCyc:G6810-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase 2UniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:glsA2UniRule annotation
Synonyms:yneH
Ordered Locus Names:b1524, JW1517
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13816. glsA2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Glutaminase 2PRO_0000110608Add
BLAST

Proteomic databases

PRIDEiP0A6W0.

Expressioni

Gene expression databases

GenevestigatoriP0A6W0.

Interactioni

Subunit structurei

Homotetramer.1 PublicationUniRule annotation

Protein-protein interaction databases

DIPiDIP-12757N.
IntActiP0A6W0. 3 interactions.
STRINGi511145.b1524.

Structurei

3D structure databases

ProteinModelPortaliP0A6W0.
SMRiP0A6W0. Positions 24-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
InParanoidiP0A6W0.
KOiK01425.
OMAiMSIAVWS.
OrthoDBiEOG6N94BK.
PhylomeDBiP0A6W0.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6W0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG
60 70 80 90 100
QLFQAGDAQE RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS
110 120 130 140 150
LVQLEMEQGI PRNPFINAGA LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD
160 170 180 190 200
ISYDTVVARS EFEHSARNAA IAWLMKSFGN FHHDVTTVLQ NYFHYCALKM
210 220 230 240 250
SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT SGMYQNAGEF
260 270 280 290 300
AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT

KQLGRSVY
Length:308
Mass (Da):33,516
Last modified:March 29, 2005 - v1
Checksum:iDDC39D9DC0D8A651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74597.1.
AP009048 Genomic DNA. Translation: BAA15206.1.
PIRiG64906.
RefSeqiNP_416041.1. NC_000913.3.
YP_489787.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74597; AAC74597; b1524.
BAA15206; BAA15206; BAA15206.
GeneIDi12932701.
944973.
KEGGiecj:Y75_p1499.
eco:b1524.
PATRICi32118346. VBIEscCol129921_1593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74597.1 .
AP009048 Genomic DNA. Translation: BAA15206.1 .
PIRi G64906.
RefSeqi NP_416041.1. NC_000913.3.
YP_489787.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A6W0.
SMRi P0A6W0. Positions 24-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12757N.
IntActi P0A6W0. 3 interactions.
STRINGi 511145.b1524.

Proteomic databases

PRIDEi P0A6W0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74597 ; AAC74597 ; b1524 .
BAA15206 ; BAA15206 ; BAA15206 .
GeneIDi 12932701.
944973.
KEGGi ecj:Y75_p1499.
eco:b1524.
PATRICi 32118346. VBIEscCol129921_1593.

Organism-specific databases

EchoBASEi EB3577.
EcoGenei EG13816. glsA2.

Phylogenomic databases

eggNOGi COG2066.
HOGENOMi HOG000216890.
InParanoidi P0A6W0.
KOi K01425.
OMAi MSIAVWS.
OrthoDBi EOG6N94BK.
PhylomeDBi P0A6W0.

Enzyme and pathway databases

BioCyci EcoCyc:G6810-MONOMER.
ECOL316407:JW1517-MONOMER.
MetaCyc:G6810-MONOMER.

Miscellaneous databases

PROi P0A6W0.

Gene expression databases

Genevestigatori P0A6W0.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF04960. Glutaminase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
    Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
    Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiGLSA2_ECOLI
AccessioniPrimary (citable) accession number: P0A6W0
Secondary accession number(s): P77470
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3