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P0A6W0 (GLSA2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase 2

EC=3.5.1.2
Gene names
Name:glsA2
Synonyms:yneH
Ordered Locus Names:b1524, JW1517
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. Ref.4

Subunit structure

Homotetramer. Ref.4

Sequence similarities

Belongs to the glutaminase family.

Biophysicochemical properties

Kinetic parameters:

KM=30.6 mM for glutamine Ref.4

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine catabolic process

Inferred from mutant phenotype Ref.4. Source: EcoCyc

negative regulation of growth

Inferred from mutant phenotype Ref.4. Source: EcoliWiki

   Molecular_functionglutaminase activity

Inferred from direct assay Ref.4. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Glutaminase 2 HAMAP-Rule MF_00313
PRO_0000110608

Sites

Binding site661Substrate By similarity
Binding site1171Substrate By similarity
Binding site1611Substrate By similarity
Binding site1681Substrate By similarity
Binding site1921Substrate By similarity
Binding site2441Substrate By similarity
Binding site2621Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A6W0 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: DDC39D9DC0D8A651

FASTA30833,516
        10         20         30         40         50         60 
MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG QLFQAGDAQE 

        70         80         90        100        110        120 
RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS LVQLEMEQGI PRNPFINAGA 

       130        140        150        160        170        180 
LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD ISYDTVVARS EFEHSARNAA IAWLMKSFGN 

       190        200        210        220        230        240 
FHHDVTTVLQ NYFHYCALKM SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT 

       250        260        270        280        290        300 
SGMYQNAGEF AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT 


KQLGRSVY 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis."
Brown G., Singer A., Proudfoot M., Skarina T., Kim Y., Chang C., Dementieva I., Kuznetsova E., Gonzalez C.F., Joachimiak A., Savchenko A., Yakunin A.F.
Biochemistry 47:5724-5735(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74597.1.
AP009048 Genomic DNA. Translation: BAA15206.1.
PIRG64906.
RefSeqNP_416041.1. NC_000913.3.
YP_489787.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A6W0.
SMRP0A6W0. Positions 24-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12757N.
IntActP0A6W0. 3 interactions.
STRING511145.b1524.

Proteomic databases

PRIDEP0A6W0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74597; AAC74597; b1524.
BAA15206; BAA15206; BAA15206.
GeneID12932701.
944973.
KEGGecj:Y75_p1499.
eco:b1524.
PATRIC32118346. VBIEscCol129921_1593.

Organism-specific databases

EchoBASEEB3577.
EcoGeneEG13816. glsA2.

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OMAVNSIMAT.
OrthoDBEOG6N94BK.
ProtClustDBPRK00971.

Enzyme and pathway databases

BioCycEcoCyc:G6810-MONOMER.
ECOL316407:JW1517-MONOMER.
MetaCyc:G6810-MONOMER.

Gene expression databases

GenevestigatorP0A6W0.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
ProtoNetSearch...

Other

PROP0A6W0.

Entry information

Entry nameGLSA2_ECOLI
AccessionPrimary (citable) accession number: P0A6W0
Secondary accession number(s): P77470
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene