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P0A6V9

- GLK_ECO57

UniProt

P0A6V9 - GLK_ECO57

Protein

Glucokinase

Gene

glk

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.

    Catalytic activityi

    ATP + D-glucose = ADP + D-glucose 6-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 136ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glucokinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-3222-MONOMER.
    ECOO157:GLK-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucokinase (EC:2.7.1.2)
    Alternative name(s):
    Glucose kinase
    Gene namesi
    Name:glk
    Ordered Locus Names:Z3654, ECs3268
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321GlucokinasePRO_0000215125Add
    BLAST

    Proteomic databases

    PRIDEiP0A6V9.

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z3654.

    Structurei

    Secondary structure

    1
    321
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 118
    Beta strandi14 – 218
    Turni22 – 243
    Beta strandi27 – 348
    Helixi35 – 373
    Helixi41 – 5111
    Beta strandi58 – 658
    Beta strandi69 – 724
    Beta strandi75 – 773
    Helixi83 – 908
    Beta strandi93 – 997
    Helixi100 – 1078
    Helixi108 – 1103
    Helixi113 – 1153
    Beta strandi116 – 1183
    Beta strandi130 – 14718
    Beta strandi150 – 1556
    Helixi158 – 1603
    Helixi168 – 18013
    Beta strandi181 – 1833
    Helixi186 – 1883
    Helixi192 – 20514
    Helixi215 – 22410
    Helixi228 – 25225
    Beta strandi257 – 2615
    Beta strandi263 – 2653
    Helixi266 – 2683
    Helixi269 – 2746
    Helixi277 – 2826
    Helixi285 – 2873
    Helixi288 – 2914
    Beta strandi296 – 2994
    Helixi304 – 31613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q18X-ray2.36A/B2-321[»]
    1SZ2X-ray2.20A/B2-321[»]
    ProteinModelPortaliP0A6V9.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A6V9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bacterial glucokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0837.
    HOGENOMiHOG000274469.
    KOiK00845.
    OMAiDWVEMTN.
    OrthoDBiEOG64BQ47.

    Family and domain databases

    HAMAPiMF_00524. Glucokinase.
    InterProiIPR003836. Glucokinase.
    [Graphical view]
    PfamiPF02685. Glucokinase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00749. glk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6V9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE    50
    EHKVEVKDGC IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND 100
    FTAVSMAIPM LKKEHLIQFG GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR 150
    WVSLPGEGGH VDFAPNSEEE AIILEILRAE IGHVSAERVL SGPGLVNLYR 200
    AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI MGRFGGNLAL 250
    NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 300
    HDNPGLLGSG AHLRQTLGHI L 321
    Length:321
    Mass (Da):34,723
    Last modified:March 29, 2005 - v1
    Checksum:i2D6CD956641A3823
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57514.1.
    BA000007 Genomic DNA. Translation: BAB36691.1.
    PIRiD91037.
    F85881.
    RefSeqiNP_288958.1. NC_002655.2.
    NP_311295.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG57514; AAG57514; Z3654.
    BAB36691; BAB36691; BAB36691.
    GeneIDi915629.
    961924.
    KEGGiece:Z3654.
    ecs:ECs3268.
    PATRICi18355936. VBIEscCol44059_3168.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57514.1 .
    BA000007 Genomic DNA. Translation: BAB36691.1 .
    PIRi D91037.
    F85881.
    RefSeqi NP_288958.1. NC_002655.2.
    NP_311295.1. NC_002695.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q18 X-ray 2.36 A/B 2-321 [» ]
    1SZ2 X-ray 2.20 A/B 2-321 [» ]
    ProteinModelPortali P0A6V9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z3654.

    Proteomic databases

    PRIDEi P0A6V9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG57514 ; AAG57514 ; Z3654 .
    BAB36691 ; BAB36691 ; BAB36691 .
    GeneIDi 915629.
    961924.
    KEGGi ece:Z3654.
    ecs:ECs3268.
    PATRICi 18355936. VBIEscCol44059_3168.

    Phylogenomic databases

    eggNOGi COG0837.
    HOGENOMi HOG000274469.
    KOi K00845.
    OMAi DWVEMTN.
    OrthoDBi EOG64BQ47.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-3222-MONOMER.
    ECOO157:GLK-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A6V9.

    Family and domain databases

    HAMAPi MF_00524. Glucokinase.
    InterProi IPR003836. Glucokinase.
    [Graphical view ]
    Pfami PF02685. Glucokinase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00749. glk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
    3. "Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose."
      Lunin V.V., Li Y., Schrag J.D., Iannuzzi P., Cygler M., Matte A.
      J. Bacteriol. 186:6915-6927(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiGLK_ECO57
    AccessioniPrimary (citable) accession number: P0A6V9
    Secondary accession number(s): P46880, P78276
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3