P0A6V9 (GLK_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucokinase
EC=2.7.1.2

Alternative name(s):
Glucose kinase

Gene names

Name:	glk
Ordered Locus Names:	Z3654, ECs3268

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. HAMAP-Rule MF_00524
Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP-Rule MF_00524
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the bacterial glucokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 321

321

Glucokinase HAMAP-Rule MF_00524

PRO_0000215125

Regions

Nucleotide binding

8 – 13

6

ATP Potential

Secondary structure

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321

Helix Strand Turn

Details...

Beta strand

4 – 11

8

Beta strand

14 – 21

8

Turn

22 – 24

3

Beta strand

27 – 34

8

Helix

35 – 37

3

Helix

41 – 51

11

Beta strand

58 – 65

8

Beta strand

69 – 72

4

Beta strand

75 – 77

3

Helix

83 – 90

8

Beta strand

93 – 99

7

Helix

100 – 107

8

Helix

108 – 110

3

Helix

113 – 115

3

Beta strand

116 – 118

3

Beta strand

130 – 147

18

Beta strand

150 – 155

6

Helix

158 – 160

3

Helix

168 – 180

13

Beta strand

181 – 183

3

Helix

186 – 188

3

Helix

192 – 205

14

Helix

215 – 224

10

Helix

228 – 252

25

Beta strand

257 – 261

5

Beta strand

263 – 265

3

Helix

266 – 268

3

Helix

269 – 274

6

Helix

277 – 282

6

Helix

285 – 287

3

Helix

288 – 291

4

Beta strand

296 – 299

4

Helix

304 – 316

13

Sequences

	Sequence		Length	Mass (Da)
	P0A6V9 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 2D6CD956641A3823	FASTA	321	34,723
	10 20 30 40 50 60 MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 70 80 90 100 110 120 IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 130 140 150 160 170 180 GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE 190 200 210 220 230 240 IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 250 260 270 280 290 300 MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 310 320 HDNPGLLGSG AHLRQTLGHI L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
[3]	"Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose." Lunin V.V., Li Y., Schrag J.D., Iannuzzi P., Cygler M., Matte A. J. Bacteriol. 186:6915-6927(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE005174 Genomic DNA. Translation: AAG57514.1.
BA000007 Genomic DNA. Translation: BAB36691.1.

PIR

D91037.
F85881.

RefSeq

NP_288958.1. NC_002655.2.
NP_311295.1. NC_002695.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q18	X-ray	2.36	A/B	2-321	[»]
1SZ2	X-ray	2.20	A/B	2-321	[»]

ProteinModelPortal

P0A6V9.

SMR

P0A6V9. Positions 2-321.

ModBase

Search...

Protein-protein interaction databases

STRING

155864.Z3654.

Proteomic databases

PRIDE

P0A6V9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAG57514; AAG57514; Z3654.
BAB36691; BAB36691; BAB36691.

GeneID

915629.
961924.

KEGG

ece:Z3654.
ecs:ECs3268.

PATRIC

18355936. VBIEscCol44059_3168.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0837.

HOGENOM

HOG000274469.

KO

K00845.

OMA

EAVIRHY.

ProtClustDB

PRK00292.

Enzyme and pathway databases

BioCyc

ECOL386585:GJFA-3222-MONOMER.

Family and domain databases

HAMAP

MF_00524. Glucokinase.

InterPro

IPR003836. Glucokinase.
[Graphical view]

Pfam

PF02685. Glucokinase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00749. glk. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A6V9.

Entry information

Entry name

GLK_ECO57

Accession

Primary (citable) accession number: P0A6V9
Secondary accession number(s): P46880, P78276

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program