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P0A6V8

- GLK_ECOLI

UniProt

P0A6V8 - GLK_ECOLI

Protein

Glucokinase

Gene

glk

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.

    Catalytic activityi

    ATP + D-glucose = ADP + D-glucose 6-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 136ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glucokinase activity Source: EcoCyc

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GLUCOKIN-MONOMER.
    ECOL316407:JW2385-MONOMER.
    MetaCyc:GLUCOKIN-MONOMER.
    SABIO-RKP0A6V8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucokinase (EC:2.7.1.2)
    Alternative name(s):
    Glucose kinase
    Gene namesi
    Name:glk
    Ordered Locus Names:b2388, JW2385
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12957. glk.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321GlucokinasePRO_0000215124Add
    BLAST

    Proteomic databases

    PaxDbiP0A6V8.
    PRIDEiP0A6V8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6V8.

    Interactioni

    Protein-protein interaction databases

    IntActiP0A6V8. 5 interactions.
    STRINGi511145.b2388.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6V8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bacterial glucokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0837.
    HOGENOMiHOG000274469.
    KOiK00845.
    OMAiDWVEMTN.
    OrthoDBiEOG64BQ47.
    PhylomeDBiP0A6V8.

    Family and domain databases

    HAMAPiMF_00524. Glucokinase.
    InterProiIPR003836. Glucokinase.
    [Graphical view]
    PfamiPF02685. Glucokinase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00749. glk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6V8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE    50
    EHKVEVKDGC IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND 100
    FTAVSMAIPM LKKEHLIQFG GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR 150
    WVSLPGEGGH VDFAPNSEEE AIILEILRAE IGHVSAERVL SGPGLVNLYR 200
    AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI MGRFGGNLAL 250
    NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 300
    HDNPGLLGSG AHLRQTLGHI L 321
    Length:321
    Mass (Da):34,723
    Last modified:March 29, 2005 - v1
    Checksum:i2D6CD956641A3823
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081I → N in AAA64506. (PubMed:9023215)Curated
    Sequence conflicti188 – 1892RV → AC in AAA64506. (PubMed:9023215)Curated
    Sequence conflicti274 – 2741A → G in AAA64506. (PubMed:9023215)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22490 Genomic DNA. Translation: AAA64506.1.
    U00096 Genomic DNA. Translation: AAC75447.1.
    AP009048 Genomic DNA. Translation: BAA16258.1.
    PIRiA65013.
    RefSeqiNP_416889.1. NC_000913.3.
    YP_490630.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75447; AAC75447; b2388.
    BAA16258; BAA16258; BAA16258.
    GeneIDi12931569.
    946858.
    KEGGiecj:Y75_p2355.
    eco:b2388.
    PATRICi32120155. VBIEscCol129921_2486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22490 Genomic DNA. Translation: AAA64506.1 .
    U00096 Genomic DNA. Translation: AAC75447.1 .
    AP009048 Genomic DNA. Translation: BAA16258.1 .
    PIRi A65013.
    RefSeqi NP_416889.1. NC_000913.3.
    YP_490630.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A6V8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A6V8. 5 interactions.
    STRINGi 511145.b2388.

    Proteomic databases

    PaxDbi P0A6V8.
    PRIDEi P0A6V8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75447 ; AAC75447 ; b2388 .
    BAA16258 ; BAA16258 ; BAA16258 .
    GeneIDi 12931569.
    946858.
    KEGGi ecj:Y75_p2355.
    eco:b2388.
    PATRICi 32120155. VBIEscCol129921_2486.

    Organism-specific databases

    EchoBASEi EB2791.
    EcoGenei EG12957. glk.

    Phylogenomic databases

    eggNOGi COG0837.
    HOGENOMi HOG000274469.
    KOi K00845.
    OMAi DWVEMTN.
    OrthoDBi EOG64BQ47.
    PhylomeDBi P0A6V8.

    Enzyme and pathway databases

    BioCyci EcoCyc:GLUCOKIN-MONOMER.
    ECOL316407:JW2385-MONOMER.
    MetaCyc:GLUCOKIN-MONOMER.
    SABIO-RK P0A6V8.

    Miscellaneous databases

    PROi P0A6V8.

    Gene expression databases

    Genevestigatori P0A6V8.

    Family and domain databases

    HAMAPi MF_00524. Glucokinase.
    InterProi IPR003836. Glucokinase.
    [Graphical view ]
    Pfami PF02685. Glucokinase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00749. glk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of glucokinase from Escherichia coli K-12."
      Meyer D., Schneider-Fresenius C., Horlacher R., Peist R., Boos W.W.
      J. Bacteriol. 179:1298-1306(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiGLK_ECOLI
    AccessioniPrimary (citable) accession number: P0A6V8
    Secondary accession number(s): P46880, P78276
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3