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Protein

Glucokinase

Gene

glk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 136ATPSequence Analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • glucokinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLUCOKIN-MONOMER.
ECOL316407:JW2385-MONOMER.
MetaCyc:GLUCOKIN-MONOMER.
SABIO-RKP0A6V8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase (EC:2.7.1.2)
Alternative name(s):
Glucose kinase
Gene namesi
Name:glk
Ordered Locus Names:b2388, JW2385
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12957. glk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321GlucokinasePRO_0000215124Add
BLAST

Proteomic databases

PaxDbiP0A6V8.
PRIDEiP0A6V8.

Interactioni

Protein-protein interaction databases

IntActiP0A6V8. 5 interactions.
STRINGi511145.b2388.

Structurei

3D structure databases

ProteinModelPortaliP0A6V8.
SMRiP0A6V8. Positions 2-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial glucokinase family.Curated

Phylogenomic databases

eggNOGiCOG0837.
HOGENOMiHOG000274469.
InParanoidiP0A6V8.
KOiK00845.
OMAiGHADFAP.
OrthoDBiEOG64BQ47.
PhylomeDBiP0A6V8.

Family and domain databases

HAMAPiMF_00524. Glucokinase.
InterProiIPR003836. Glucokinase.
[Graphical view]
PfamiPF02685. Glucokinase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00749. glk. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE
60 70 80 90 100
EHKVEVKDGC IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND
110 120 130 140 150
FTAVSMAIPM LKKEHLIQFG GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR
160 170 180 190 200
WVSLPGEGGH VDFAPNSEEE AIILEILRAE IGHVSAERVL SGPGLVNLYR
210 220 230 240 250
AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI MGRFGGNLAL
260 270 280 290 300
NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV
310 320
HDNPGLLGSG AHLRQTLGHI L
Length:321
Mass (Da):34,723
Last modified:March 29, 2005 - v1
Checksum:i2D6CD956641A3823
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081I → N in AAA64506 (PubMed:9023215).Curated
Sequence conflicti188 – 1892RV → AC in AAA64506 (PubMed:9023215).Curated
Sequence conflicti274 – 2741A → G in AAA64506 (PubMed:9023215).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22490 Genomic DNA. Translation: AAA64506.1.
U00096 Genomic DNA. Translation: AAC75447.1.
AP009048 Genomic DNA. Translation: BAA16258.1.
PIRiA65013.
RefSeqiNP_416889.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75447; AAC75447; b2388.
BAA16258; BAA16258; BAA16258.
GeneIDi946858.
KEGGiecj:Y75_p2355.
eco:b2388.
PATRICi32120155. VBIEscCol129921_2486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22490 Genomic DNA. Translation: AAA64506.1.
U00096 Genomic DNA. Translation: AAC75447.1.
AP009048 Genomic DNA. Translation: BAA16258.1.
PIRiA65013.
RefSeqiNP_416889.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A6V8.
SMRiP0A6V8. Positions 2-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0A6V8. 5 interactions.
STRINGi511145.b2388.

Proteomic databases

PaxDbiP0A6V8.
PRIDEiP0A6V8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75447; AAC75447; b2388.
BAA16258; BAA16258; BAA16258.
GeneIDi946858.
KEGGiecj:Y75_p2355.
eco:b2388.
PATRICi32120155. VBIEscCol129921_2486.

Organism-specific databases

EchoBASEiEB2791.
EcoGeneiEG12957. glk.

Phylogenomic databases

eggNOGiCOG0837.
HOGENOMiHOG000274469.
InParanoidiP0A6V8.
KOiK00845.
OMAiGHADFAP.
OrthoDBiEOG64BQ47.
PhylomeDBiP0A6V8.

Enzyme and pathway databases

BioCyciEcoCyc:GLUCOKIN-MONOMER.
ECOL316407:JW2385-MONOMER.
MetaCyc:GLUCOKIN-MONOMER.
SABIO-RKP0A6V8.

Miscellaneous databases

PROiP0A6V8.

Family and domain databases

HAMAPiMF_00524. Glucokinase.
InterProiIPR003836. Glucokinase.
[Graphical view]
PfamiPF02685. Glucokinase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00749. glk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of glucokinase from Escherichia coli K-12."
    Meyer D., Schneider-Fresenius C., Horlacher R., Peist R., Boos W.W.
    J. Bacteriol. 179:1298-1306(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLK_ECOLI
AccessioniPrimary (citable) accession number: P0A6V8
Secondary accession number(s): P46880, P78276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: June 24, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.