P0A6V8 (GLK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucokinase
EC=2.7.1.2

Alternative name(s):
Glucose kinase

Gene names

Name:	glk
Ordered Locus Names:	b2388, JW2385

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. HAMAP-Rule MF_00524
Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP-Rule MF_00524
Subcellular location	Cytoplasm HAMAP-Rule MF_00524.
Sequence similarities	Belongs to the bacterial glucokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate catabolic process Inferred from genetic interaction PubMed 19028900. Source: EcoCyc glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from direct assay PubMed 17307338. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Glucokinase HAMAP-Rule MF_00524		PRO_0000215124
Regions
	Nucleotide binding	8 – 13	6	ATP Potential
Experimental info
	Sequence conflict	108	1	I → N in AAA64506. Ref.1
	Sequence conflict	188 – 189	2	RV → AC in AAA64506. Ref.1
	Sequence conflict	274	1	A → G in AAA64506. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A6V8 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 2D6CD956641A3823	FASTA	321	34,723
	10 20 30 40 50 60 MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 70 80 90 100 110 120 IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 130 140 150 160 170 180 GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE 190 200 210 220 230 240 IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 250 260 270 280 290 300 MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 310 320 HDNPGLLGSG AHLRQTLGHI L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of glucokinase from Escherichia coli K-12." Meyer D., Schneider-Fresenius C., Horlacher R., Peist R., Boos W.W. J. Bacteriol. 179:1298-1306(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22490 Genomic DNA. Translation: AAA64506.1. U00096 Genomic DNA. Translation: AAC75447.1. AP009048 Genomic DNA. Translation: BAA16258.1.
PIR	A65013.
RefSeq	NP_416889.1. NC_000913.2. YP_490630.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A6V8.
SMR	P0A6V8. Positions 2-321.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A6V8. 5 interactions.
STRING	511145.b2388.
Proteomic databases
PaxDb	P0A6V8.
PRIDE	P0A6V8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75447; AAC75447; b2388. BAA16258; BAA16258; BAA16258.
GeneID	12931569. 946858.
KEGG	ecj:Y75_p2355. eco:b2388.
PATRIC	32120155. VBIEscCol129921_2486.
Organism-specific databases
EchoBASE	EB2791.
EcoGene	EG12957. glk.
Phylogenomic databases
eggNOG	COG0837.
HOGENOM	HOG000274469.
KO	K00845.
OMA	EAVIRHY.
ProtClustDB	PRK00292.
Enzyme and pathway databases
BioCyc	EcoCyc:GLUCOKIN-MONOMER. ECOL316407:JW2385-MONOMER. MetaCyc:GLUCOKIN-MONOMER.
SABIO-RK	P0A6V8.
Gene expression databases
Genevestigator	P0A6V8.
Family and domain databases
HAMAP	MF_00524. Glucokinase.
InterPro	IPR003836. Glucokinase. [Graphical view]
Pfam	PF02685. Glucokinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00749. glk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLK_ECOLI

Accession

Primary (citable) accession number: P0A6V8
Secondary accession number(s): P46880, P78276

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program