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P0A6V8 (GLK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucokinase

EC=2.7.1.2
Alternative name(s):
Glucose kinase
Gene names
Name:glk
Ordered Locus Names:b2388, JW2385
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. HAMAP-Rule MF_00524

Catalytic activity

ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP-Rule MF_00524

Subcellular location

Cytoplasm HAMAP-Rule MF_00524.

Sequence similarities

Belongs to the bacterial glucokinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glucokinase activity

Inferred from direct assay PubMed 17307338. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321Glucokinase HAMAP-Rule MF_00524
PRO_0000215124

Regions

Nucleotide binding8 – 136ATP Potential

Experimental info

Sequence conflict1081I → N in AAA64506. Ref.1
Sequence conflict188 – 1892RV → AC in AAA64506. Ref.1
Sequence conflict2741A → G in AAA64506. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0A6V8 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: 2D6CD956641A3823

FASTA32134,723
        10         20         30         40         50         60 
MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 

        70         80         90        100        110        120 
IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 

       130        140        150        160        170        180 
GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE 

       190        200        210        220        230        240 
IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 

       250        260        270        280        290        300 
MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 

       310        320 
HDNPGLLGSG AHLRQTLGHI L 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of glucokinase from Escherichia coli K-12."
Meyer D., Schneider-Fresenius C., Horlacher R., Peist R., Boos W.W.
J. Bacteriol. 179:1298-1306(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22490 Genomic DNA. Translation: AAA64506.1.
U00096 Genomic DNA. Translation: AAC75447.1.
AP009048 Genomic DNA. Translation: BAA16258.1.
PIRA65013.
RefSeqNP_416889.1. NC_000913.3.
YP_490630.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A6V8.
SMRP0A6V8. Positions 2-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0A6V8. 5 interactions.
STRING511145.b2388.

Proteomic databases

PaxDbP0A6V8.
PRIDEP0A6V8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75447; AAC75447; b2388.
BAA16258; BAA16258; BAA16258.
GeneID12931569.
946858.
KEGGecj:Y75_p2355.
eco:b2388.
PATRIC32120155. VBIEscCol129921_2486.

Organism-specific databases

EchoBASEEB2791.
EcoGeneEG12957. glk.

Phylogenomic databases

eggNOGCOG0837.
HOGENOMHOG000274469.
KOK00845.
OMADWVEMTN.
OrthoDBEOG64BQ47.
ProtClustDBPRK00292.

Enzyme and pathway databases

BioCycEcoCyc:GLUCOKIN-MONOMER.
ECOL316407:JW2385-MONOMER.
MetaCyc:GLUCOKIN-MONOMER.
SABIO-RKP0A6V8.

Gene expression databases

GenevestigatorP0A6V8.

Family and domain databases

HAMAPMF_00524. Glucokinase.
InterProIPR003836. Glucokinase.
[Graphical view]
PfamPF02685. Glucokinase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00749. glk. 1 hit.
ProtoNetSearch...

Other

PROP0A6V8.

Entry information

Entry nameGLK_ECOLI
AccessionPrimary (citable) accession number: P0A6V8
Secondary accession number(s): P46880, P78276
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene