P0A6V7 (GLPE_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	SF3447, S4317

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.
Sequence caution	The sequence AAN44907.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_0000200566
Regions
	Domain	17 – 105	89	Rhodanese
Sites
	Active site	65	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A6V7 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 18D5B461C2A8EF19	FASTA	108	12,082
	10 20 30 40 50 60 MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAVQA FHLTNDTLGA FMRDNDFDTP 70 80 90 100 VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF PAEVAYGA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN44907.1. Different initiation. AE014073 Genomic DNA. Translation: AAP19274.1.
RefSeq	NP_709200.2. NC_004337.2. NP_839463.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0A6V7.
SMR	P0A6V7. Positions 1-108.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000085194; EBESCP00000082016; EBESCG00000084240. EBESCT00000090078; EBESCP00000086714; EBESCG00000089122.
GeneID	1026510. 1080921.
GenomeReviews	Gene locus SF3447 in contig AE005674_GR. Gene locus S4317 in contig AE014073_GR.
KEGG	sfl:SF3447. sfx:S4317.
PATRIC	18710628. VBIShiFle31049_4718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010658.
HOGENOM	HBG743107.
ProtClustDB	PRK00162.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44907.1-MONOMER.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
KO	K02439.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_SHIFL

Accession

Primary (citable) accession number: P0A6V7
Secondary accession number(s): P09390, Q47235

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program