Thiosulfate sulfurtransferase glpE - Escherichia coli (strain K12)

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P0A6V5 (GLPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	b3425, JW3388

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of glpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP MF_01009.
Induction	By infection by filamentous bacteriophages. Expression is positively regulated by cyclic AMP-cAMP receptor protein (cAMP-CRP). Ref.5 Ref.7
Miscellaneous	Incubation of glpE with the cysteine-specific modifying reagent DTNB resulted in a greater-than-90% loss of activity. HAMAP MF_01009
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.
Sequence caution	The sequence AAA23889.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.6. Source: EcoCyc
Molecular function	thiosulfate sulfurtransferase activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 108

108

Thiosulfate sulfurtransferase glpE HAMAP MF_01009

PRO_0000200549

Regions

Domain

17 – 105

Rhodanese

Sites

Active site

Cysteine persulfide intermediate Probable

Experimental info

Sequence conflict

108

A → RNALYCPLLCGISDSNDVDD YLFC Ref.2

Secondary structure

108

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A6V5 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: 18D5B461C2A8EF19
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FASTA

108

12,082

        10         20         30         40         50         60 
MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAVQA FHLTNDTLGA FMRDNDFDTP 

        70         80         90        100 
VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF PAEVAYGA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain." Zeng G., Ye S., Larson T.J. J. Bacteriol. 178:7080-7089(1996) [PubMed: 8955387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12." Choi Y.-L., Kawase S., Nishida T., Sakai H., Komano T., Kawamukai M., Utsumi R., Kohara Y., Akiyama K. Nucleic Acids Res. 16:7732-7732(1988) [PubMed: 3045764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of glpD and glpE gene expression by a cyclic AMP-cAMP receptor protein (cAMP-CRP) complex in Escherichia coli." Choi Y.-L., Kawase S., Kawamukai M., Sakai H., Komano T. Biochim. Biophys. Acta 1088:31-35(1991) [PubMed: 1846566] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin." Ray W.K., Zeng G., Potters M.B., Mansuri A.M., Larson T.J. J. Bacteriol. 182:2277-2284(2000) [PubMed: 10735872] [Abstract] Cited for: CHARACTERIZATION. Strain: BL21-DE3.
[7]	"Biochemical and genetic characterization of pspE and glpE, two single-domain sulfurtransferases of Escherichia coli." Cheng H., Donahue J.L., Battle S.E., Ray W.K., Larson T.J. Open Microbiol. J. 2:18-28(2008) [PubMed: 19088907] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[8]	"Crystals of GlpE, a 12 kDa sulfurtransferase from Escherichia coli, display 1.06 A resolution diffraction: a preliminary report." Bordo D., Larson T.J., Donahue J.L., Spallarossa A., Bolognesi M. Acta Crystallogr. D 56:1691-1693(2000) [PubMed: 11092948] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS). Strain: BL21-DE3.
[9]	"Escherichia coli GlpE is a prototype sulfurtransferase for the single-domain rhodanese homology superfamily." Spallarossa A., Donahue J.L., Larson T.J., Bolognesi M., Bordo D. Structure 9:1117-1125(2001) [PubMed: 11709175] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS). Strain: BL21-DE3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M96795 Genomic DNA. Translation: AAC28165.1.
X07520 Genomic DNA. Translation: CAA30397.1.
M54940 Genomic DNA. Translation: AAA23889.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58223.1.
U00096 Genomic DNA. Translation: AAC76450.1.
AP009048 Genomic DNA. Translation: BAE77867.1.

PIR

BVECGE. D65138.

RefSeq

NP_417883.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GMX	X-ray	1.10	A	1-108	[»]
1GN0	X-ray	1.80	A	1-108	[»]

ProteinModelPortal

P0A6V5.

SMR

P0A6V5. Positions 1-108.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A6V5. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002575; EBESCP00000002575; EBESCG00000002101.
EBESCT00000017761; EBESCP00000017052; EBESCG00000016817.

GeneID

947935.

GenomeReviews

Gene locus JW3388 in contig AP009048_GR.
Gene locus b3425 in contig U00096_GR.

KEGG

ecj:JW3388.
eco:b3425.

PATRIC

32122286. VBIEscCol129921_3520.

Organism-specific databases

EchoBASE

EB0390.

EcoGene

EG10395. glpE.

Phylogenomic databases

eggNOG

COG0607.

GeneTree

EBGT00050000010658.

HOGENOM

HBG743107.

OMA

FSAWHEA.

PhylomeDB

P0A6V5.

ProtClustDB

PRK00162.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10395-MONOMER.
MetaCyc:EG10395-MONOMER.

Gene expression databases

Genevestigator

P0A6V5.

Family and domain databases

HAMAP

MF_01009. Thiosulf_sulfurtr.
[Tree]

InterPro

IPR001763. Rhodanese-like.
IPR023695. Thiosulf_sulfurTrfase.
[Graphical view]

Gene3D

G3DSA:3.40.250.10. Rhodanese-like. 1 hit.

K02439.

Pfam

PF00581. Rhodanese. 1 hit.
[Graphical view]

SMART

SM00450. RHOD. 1 hit.
[Graphical view]

SUPFAM

SSF52821. Rhodanese-like. 1 hit.

PROSITE

PS50206. RHODANESE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GLPE_ECOLI

Accession

Primary (citable) accession number: P0A6V5
Secondary accession number(s): P09390, Q2M789, Q47235

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents