P0A6V5 (GLPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase GlpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	b3425, JW3388

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated. HAMAP-Rule MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP-Rule MF_01009
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP-Rule MF_01009.
Induction	By infection by filamentous bacteriophages. Expression is positively regulated by cyclic AMP-cAMP receptor protein (cAMP-CRP). Ref.5 Ref.7
Miscellaneous	Incubation of GlpE with the cysteine-specific modifying reagent DTNB resulted in a greater-than-90% loss of activity. HAMAP-Rule MF_01009
Sequence similarities	Belongs to the GlpE family. Contains 1 rhodanese domain.
Sequence caution	The sequence AAA23889.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: EcoCyc
Molecular_function	thiosulfate sulfurtransferase activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase GlpE HAMAP-Rule MF_01009		PRO_0000200549
Regions
	Domain	17 – 105	89	Rhodanese
Sites
	Active site	65	1	Cysteine persulfide intermediate Probable
Experimental info
	Sequence conflict	108	1	A → RNALYCPLLCGISDSNDVDD YLFC Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A6V5 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 18D5B461C2A8EF19	FASTA	108	12,082
	10 20 30 40 50 60 MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAVQA FHLTNDTLGA FMRDNDFDTP 70 80 90 100 VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF PAEVAYGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain." Zeng G., Ye S., Larson T.J. J. Bacteriol. 178:7080-7089(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12." Choi Y.-L., Kawase S., Nishida T., Sakai H., Komano T., Kawamukai M., Utsumi R., Kohara Y., Akiyama K. Nucleic Acids Res. 16:7732-7732(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of glpD and glpE gene expression by a cyclic AMP-cAMP receptor protein (cAMP-CRP) complex in Escherichia coli." Choi Y.-L., Kawase S., Kawamukai M., Sakai H., Komano T. Biochim. Biophys. Acta 1088:31-35(1991) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin." Ray W.K., Zeng G., Potters M.B., Mansuri A.M., Larson T.J. J. Bacteriol. 182:2277-2284(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: BL21-DE3.
[7]	"Biochemical and genetic characterization of pspE and glpE, two single-domain sulfurtransferases of Escherichia coli." Cheng H., Donahue J.L., Battle S.E., Ray W.K., Larson T.J. Open Microbiol. J. 2:18-28(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[8]	"Crystals of GlpE, a 12 kDa sulfurtransferase from Escherichia coli, display 1.06 A resolution diffraction: a preliminary report." Bordo D., Larson T.J., Donahue J.L., Spallarossa A., Bolognesi M. Acta Crystallogr. D 56:1691-1693(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS). Strain: BL21-DE3.
[9]	"Escherichia coli GlpE is a prototype sulfurtransferase for the single-domain rhodanese homology superfamily." Spallarossa A., Donahue J.L., Larson T.J., Bolognesi M., Bordo D. Structure 9:1117-1125(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS). Strain: BL21-DE3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96795 Genomic DNA. Translation: AAC28165.1. X07520 Genomic DNA. Translation: CAA30397.1. M54940 Genomic DNA. Translation: AAA23889.1. Different initiation. U18997 Genomic DNA. Translation: AAA58223.1. U00096 Genomic DNA. Translation: AAC76450.1. AP009048 Genomic DNA. Translation: BAE77867.1.
PIR	BVECGE. D65138.
RefSeq	NP_417883.1. NC_000913.2. YP_492008.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A6V5.
SMR	P0A6V5. Positions 1-108.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A6V5. 2 interactions.
STRING	511145.b3425.
Proteomic databases
PaxDb	P0A6V5.
PRIDE	P0A6V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76450; AAC76450; b3425. BAE77867; BAE77867; BAE77867.
GeneID	12933493. 947935.
KEGG	ecj:Y75_p3752. eco:b3425.
PATRIC	32122286. VBIEscCol129921_3520.
Organism-specific databases
EchoBASE	EB0390.
EcoGene	EG10395. glpE.
Phylogenomic databases
eggNOG	COG0607.
HOGENOM	HOG000247776.
KO	K02439.
OMA	FSAWHEA.
ProtClustDB	PRK00162.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10395-MONOMER. ECOL316407:JW3388-MONOMER. MetaCyc:EG10395-MONOMER.
Gene expression databases
Genevestigator	P0A6V5.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
HAMAP	MF_01009. Thiosulf_sulfurtr.
InterPro	IPR001763. Rhodanese-like_dom. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
EvolutionaryTrace	P0A6V5.

Entry information

Entry name

GLPE_ECOLI

Accession

Primary (citable) accession number: P0A6V5
Secondary accession number(s): P09390, Q2M789, Q47235

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program