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P0A6V5

- GLPE_ECOLI

UniProt

P0A6V5 - GLPE_ECOLI

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Protein

Thiosulfate sulfurtransferase GlpE

Gene
glpE, b3425, JW3388
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated.UniRule annotation

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Cysteine persulfide intermediate Inferred

GO - Molecular functioni

  1. thiosulfate sulfurtransferase activity Source: EcoCyc

GO - Biological processi

  1. glycerol metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG10395-MONOMER.
ECOL316407:JW3388-MONOMER.
MetaCyc:EG10395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase GlpE (EC:2.8.1.1)
Gene namesi
Name:glpE
Ordered Locus Names:b3425, JW3388
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10395. glpE.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 108108Thiosulfate sulfurtransferase GlpEUniRule annotationPRO_0000200549Add
BLAST

Proteomic databases

PaxDbiP0A6V5.
PRIDEiP0A6V5.

Expressioni

Inductioni

By infection by filamentous bacteriophages. Expression is positively regulated by cyclic AMP-cAMP receptor protein (cAMP-CRP).2 Publications

Gene expression databases

GenevestigatoriP0A6V5.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP0A6V5. 2 interactions.
STRINGi511145.b3425.

Structurei

Secondary structure

1
108
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73
Helixi9 – 179
Beta strandi22 – 254
Helixi29 – 346
Helixi45 – 5410
Beta strandi61 – 644
Beta strandi66 – 694
Helixi70 – 8112
Beta strandi84 – 896
Helixi92 – 998
Helixi101 – 1033

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GMXX-ray1.10A1-108[»]
1GN0X-ray1.80A1-108[»]
ProteinModelPortaliP0A6V5.
SMRiP0A6V5. Positions 1-108.

Miscellaneous databases

EvolutionaryTraceiP0A6V5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 10589RhodaneseAdd
BLAST

Sequence similaritiesi

Belongs to the GlpE family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG0607.
HOGENOMiHOG000247776.
KOiK02439.
OMAiILVMCYH.
OrthoDBiEOG68DD6K.
PhylomeDBiP0A6V5.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
HAMAPiMF_01009. Thiosulf_sulfurtr.
InterProiIPR001763. Rhodanese-like_dom.
IPR023695. Thiosulf_sulfurTrfase.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6V5-1 [UniParc]FASTAAdd to Basket

« Hide

MDQFECINVA DAHQKLQEKE AVLVDIRDPQ SFAMGHAVQA FHLTNDTLGA    50
FMRDNDFDTP VMVMCYHGNS SKGAAQYLLQ QGYDVVYSID GGFEAWQRQF 100
PAEVAYGA 108
Length:108
Mass (Da):12,082
Last modified:March 29, 2005 - v1
Checksum:i18D5B461C2A8EF19
GO

Sequence cautioni

The sequence AAA23889.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081A → RNALYCPLLCGISDSNDVDD YLFC1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96795 Genomic DNA. Translation: AAC28165.1.
X07520 Genomic DNA. Translation: CAA30397.1.
M54940 Genomic DNA. Translation: AAA23889.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58223.1.
U00096 Genomic DNA. Translation: AAC76450.1.
AP009048 Genomic DNA. Translation: BAE77867.1.
PIRiD65138. BVECGE.
RefSeqiNP_417883.1. NC_000913.3.
YP_492008.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76450; AAC76450; b3425.
BAE77867; BAE77867; BAE77867.
GeneIDi12933493.
947935.
KEGGiecj:Y75_p3752.
eco:b3425.
PATRICi32122286. VBIEscCol129921_3520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96795 Genomic DNA. Translation: AAC28165.1 .
X07520 Genomic DNA. Translation: CAA30397.1 .
M54940 Genomic DNA. Translation: AAA23889.1 . Different initiation.
U18997 Genomic DNA. Translation: AAA58223.1 .
U00096 Genomic DNA. Translation: AAC76450.1 .
AP009048 Genomic DNA. Translation: BAE77867.1 .
PIRi D65138. BVECGE.
RefSeqi NP_417883.1. NC_000913.3.
YP_492008.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GMX X-ray 1.10 A 1-108 [» ]
1GN0 X-ray 1.80 A 1-108 [» ]
ProteinModelPortali P0A6V5.
SMRi P0A6V5. Positions 1-108.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0A6V5. 2 interactions.
STRINGi 511145.b3425.

Proteomic databases

PaxDbi P0A6V5.
PRIDEi P0A6V5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76450 ; AAC76450 ; b3425 .
BAE77867 ; BAE77867 ; BAE77867 .
GeneIDi 12933493.
947935.
KEGGi ecj:Y75_p3752.
eco:b3425.
PATRICi 32122286. VBIEscCol129921_3520.

Organism-specific databases

EchoBASEi EB0390.
EcoGenei EG10395. glpE.

Phylogenomic databases

eggNOGi COG0607.
HOGENOMi HOG000247776.
KOi K02439.
OMAi ILVMCYH.
OrthoDBi EOG68DD6K.
PhylomeDBi P0A6V5.

Enzyme and pathway databases

BioCyci EcoCyc:EG10395-MONOMER.
ECOL316407:JW3388-MONOMER.
MetaCyc:EG10395-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A6V5.
PROi P0A6V5.

Gene expression databases

Genevestigatori P0A6V5.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
HAMAPi MF_01009. Thiosulf_sulfurtr.
InterProi IPR001763. Rhodanese-like_dom.
IPR023695. Thiosulf_sulfurTrfase.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain."
    Zeng G., Ye S., Larson T.J.
    J. Bacteriol. 178:7080-7089(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12."
    Choi Y.-L., Kawase S., Nishida T., Sakai H., Komano T., Kawamukai M., Utsumi R., Kohara Y., Akiyama K.
    Nucleic Acids Res. 16:7732-7732(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Regulation of glpD and glpE gene expression by a cyclic AMP-cAMP receptor protein (cAMP-CRP) complex in Escherichia coli."
    Choi Y.-L., Kawase S., Kawamukai M., Sakai H., Komano T.
    Biochim. Biophys. Acta 1088:31-35(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin."
    Ray W.K., Zeng G., Potters M.B., Mansuri A.M., Larson T.J.
    J. Bacteriol. 182:2277-2284(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: BL21-DE3.
  7. "Biochemical and genetic characterization of pspE and glpE, two single-domain sulfurtransferases of Escherichia coli."
    Cheng H., Donahue J.L., Battle S.E., Ray W.K., Larson T.J.
    Open Microbiol. J. 2:18-28(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "Crystals of GlpE, a 12 kDa sulfurtransferase from Escherichia coli, display 1.06 A resolution diffraction: a preliminary report."
    Bordo D., Larson T.J., Donahue J.L., Spallarossa A., Bolognesi M.
    Acta Crystallogr. D 56:1691-1693(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS).
    Strain: BL21-DE3.
  9. "Escherichia coli GlpE is a prototype sulfurtransferase for the single-domain rhodanese homology superfamily."
    Spallarossa A., Donahue J.L., Larson T.J., Bolognesi M., Bordo D.
    Structure 9:1117-1125(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS).
    Strain: BL21-DE3.

Entry informationi

Entry nameiGLPE_ECOLI
AccessioniPrimary (citable) accession number: P0A6V5
Secondary accession number(s): P09390, Q2M789, Q47235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Incubation of GlpE with the cysteine-specific modifying reagent DTNB resulted in a greater-than-90% loss of activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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