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Protein

Glucose-1-phosphate adenylyltransferase

Gene

glgC

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.UniRule annotation

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose-1,6-bisphosphate (F16BP) and inhibited by AMP.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Fructose-1,6-bisphosphateUniRule annotation1
Binding sitei40AMPUniRule annotation1
Binding sitei46AMPUniRule annotation1
Binding sitei52AMPUniRule annotation1
Sitei74Could play a key role in the communication between the regulatory and the substrate sitesUniRule annotation1
Sitei113Could play a key role in the communication between the regulatory and the substrate sitesUniRule annotation1
Binding sitei114Alpha-D-glucose 1-phosphateUniRule annotation1
Binding sitei130AMPUniRule annotation1
Binding sitei179Alpha-D-glucose 1-phosphate; via amide nitrogenUniRule annotation1
Binding sitei212Alpha-D-glucose 1-phosphate; via carbonyl oxygenUniRule annotation1
Binding sitei370AMPUniRule annotation1
Binding sitei386AMPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.27UniRule annotation)
Alternative name(s):
ADP-glucose pyrophosphorylaseUniRule annotation
Short name:
ADPGlc PPaseUniRule annotation
ADP-glucose synthaseUniRule annotation
Gene namesi
Name:glgCUniRule annotation
Ordered Locus Names:Z4792, ECs4275
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001952962 – 431Glucose-1-phosphate adenylyltransferaseAdd BLAST430

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

MINTiMINT-1250907.
STRINGi155864.Z4792.

Structurei

3D structure databases

ProteinModelPortaliP0A6V3.
SMRiP0A6V3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 195Alpha-D-glucose 1-phosphate bindingUniRule annotation2
Regioni419 – 423Fructose-1,6-bisphosphate bindingUniRule annotation5
Regioni429 – 431Fructose-1,6-bisphosphate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
KOiK00975.
OMAiFGCIDSD.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiView protein in InterPro
IPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
PfamiView protein in Pfam
PF00483. NTP_transferase. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiView protein in PROSITE
PS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6V3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLEKNDHL MLARQLPLKS VALILAGGRG TRLKDLTNKR AKPAVHFGGK
60 70 80 90 100
FRIIDFALSN CINSGIRRMG VITQYQSHTL VQHIQRGWSF FNEEMNEFVD
110 120 130 140 150
LLPAQQRMKG ENWYRGTADA VTQNLDIIRR YKAEYVVILA GDHIYKQDYS
160 170 180 190 200
RMLIDHVEKG ARCTVACMPV PIEEASAFGV MAVDENDKII EFVEKPANPP
210 220 230 240 250
SMPNDPSKSL ASMGIYVFDA DYLYELLEED DRDENSSHDF GKDLIPKITE
260 270 280 290 300
AGLAYAHPFP LSCVQSDPDA EPYWRDVGTL EAYWKANLDL ASVVPELDMY
310 320 330 340 350
DRNWPIRTYN ESLPPAKFVQ DRSGSHGMTL NSLVSGGCVI SGSVVVQSVL
360 370 380 390 400
FSRVRVNSFC NIDSAVLLPE VWVGRSCRLR RCVIDRACVI PEGMVIGENA
410 420 430
EEDARRFYRS EEGIVLVTRE MLRKLGHKQE R
Length:431
Mass (Da):48,698
Last modified:January 23, 2007 - v2
Checksum:i3C0A4C4F5B13D9D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58536.1.
BA000007 Genomic DNA. Translation: BAB37698.1.
PIRiC91163.
D86009.
RefSeqiNP_312302.1. NC_002695.1.
WP_000253975.1. NZ_MKIV01000001.1.

Genome annotation databases

EnsemblBacteriaiAAG58536; AAG58536; Z4792.
BAB37698; BAB37698; BAB37698.
GeneIDi915871.
KEGGiece:Z4792.
ecs:ECs4275.
PATRICi18358115. VBIEscCol44059_4224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58536.1.
BA000007 Genomic DNA. Translation: BAB37698.1.
PIRiC91163.
D86009.
RefSeqiNP_312302.1. NC_002695.1.
WP_000253975.1. NZ_MKIV01000001.1.

3D structure databases

ProteinModelPortaliP0A6V3.
SMRiP0A6V3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1250907.
STRINGi155864.Z4792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58536; AAG58536; Z4792.
BAB37698; BAB37698; BAB37698.
GeneIDi915871.
KEGGiece:Z4792.
ecs:ECs4275.
PATRICi18358115. VBIEscCol44059_4224.

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
KOiK00975.
OMAiFGCIDSD.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiView protein in InterPro
IPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
PfamiView protein in Pfam
PF00483. NTP_transferase. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiView protein in PROSITE
PS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGC_ECO57
AccessioniPrimary (citable) accession number: P0A6V3
Secondary accession number(s): P00584
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.