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Protein

Glucose-1-phosphate adenylyltransferase

Gene

glgC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans.

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.

Enzyme regulationi

Activated by fructose-1,6-bisphosphate and inhibited by AMP and ADP. Allosteric regulation.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Allosteric activator
Binding sitei114 – 1141Glucose-1-phosphate
Binding sitei195 – 1951Glucose-1-phosphate

GO - Molecular functioni

  • AMP binding Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • glucose-1-phosphate adenylyltransferase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • glycogen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLUC1PADENYLTRANS-MONOMER.
ECOL316407:JW3393-MONOMER.
MetaCyc:GLUC1PADENYLTRANS-MONOMER.
BRENDAi2.7.7.27. 2026.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferase (EC:2.7.7.27)
Alternative name(s):
ADP-glucose pyrophosphorylase
Short name:
ADPGlc PPase
ADP-glucose synthase
Gene namesi
Name:glgC
Ordered Locus Names:b3430, JW3393
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10379. glgC.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi195 – 1951K → E, I, H, Q or R: No loss in enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 431430Glucose-1-phosphate adenylyltransferasePRO_0000195294Add
BLAST

Proteomic databases

EPDiP0A6V1.
PaxDbiP0A6V1.
PRIDEiP0A6V1.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4262502. 259 interactions.
DIPiDIP-48147N.
IntActiP0A6V1. 6 interactions.
STRINGi511145.b3430.

Structurei

3D structure databases

ProteinModelPortaliP0A6V1.
SMRiP0A6V1. Positions 16-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
InParanoidiP0A6V1.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP0A6V1.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLEKNDHL MLARQLPLKS VALILAGGRG TRLKDLTNKR AKPAVHFGGK
60 70 80 90 100
FRIIDFALSN CINSGIRRMG VITQYQSHTL VQHIQRGWSF FNEEMNEFVD
110 120 130 140 150
LLPAQQRMKG ENWYRGTADA VTQNLDIIRR YKAEYVVILA GDHIYKQDYS
160 170 180 190 200
RMLIDHVEKG ARCTVACMPV PIEEASAFGV MAVDENDKII EFVEKPANPP
210 220 230 240 250
SMPNDPSKSL ASMGIYVFDA DYLYELLEED DRDENSSHDF GKDLIPKITE
260 270 280 290 300
AGLAYAHPFP LSCVQSDPDA EPYWRDVGTL EAYWKANLDL ASVVPELDMY
310 320 330 340 350
DRNWPIRTYN ESLPPAKFVQ DRSGSHGMTL NSLVSGGCVI SGSVVVQSVL
360 370 380 390 400
FSRVRVNSFC NIDSAVLLPE VWVGRSCRLR RCVIDRACVI PEGMVIGENA
410 420 430
EEDARRFYRS EEGIVLVTRE MLRKLGHKQE R
Length:431
Mass (Da):48,698
Last modified:January 23, 2007 - v2
Checksum:i3C0A4C4F5B13D9D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611A → V in CAA23544 (PubMed:1320612).Curated
Sequence conflicti161 – 1611A → V in AAA98736 (PubMed:1320612).Curated
Sequence conflicti161 – 1611A → V in AAA23873 (PubMed:1320612).Curated
Sequence conflicti166 – 1661A → V in CAA23544 (PubMed:1320612).Curated
Sequence conflicti166 – 1661A → V in AAA98736 (PubMed:1320612).Curated
Sequence conflicti166 – 1661A → V in AAA23873 (PubMed:1320612).Curated
Sequence conflicti189 – 1891I → T in CAA23544 (PubMed:1320612).Curated
Sequence conflicti189 – 1891I → T in AAA98736 (PubMed:1320612).Curated
Sequence conflicti189 – 1891I → T in AAA23873 (PubMed:1320612).Curated
Sequence conflicti296 – 2961E → K in CAA23544 (PubMed:1320612).Curated
Sequence conflicti296 – 2961E → K in AAA98736 (PubMed:1320612).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441A → T in SG14 mutant; lower apparent affinities for substrates, the activator, F16BP and the inhibitor.
Natural varianti67 – 671R → C in CL1136 mutant; less dependent on the allosteric activator, F16BP, for activity and less sensitive to inhibition by AMP.
Natural varianti295 – 2951P → S in SG5 mutant.
Natural varianti336 – 3361G → D in 618 mutant; causes lowered affinity for AMP.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00281 Genomic DNA. Translation: CAA23544.1.
J01616 Genomic DNA. Translation: AAA98736.1.
M97226 Genomic DNA. Translation: AAA23873.1.
S58224 Genomic DNA. Translation: AAB26162.1.
U18997 Genomic DNA. Translation: AAA58228.1.
U00096 Genomic DNA. Translation: AAC76455.1.
AP009048 Genomic DNA. Translation: BAE77862.1.
PIRiA00721. YUEC.
RefSeqiNP_417888.1. NC_000913.3.
WP_000253975.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76455; AAC76455; b3430.
BAE77862; BAE77862; BAE77862.
GeneIDi947942.
KEGGiecj:JW3393.
eco:b3430.
PATRICi32122300. VBIEscCol129921_3527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00281 Genomic DNA. Translation: CAA23544.1.
J01616 Genomic DNA. Translation: AAA98736.1.
M97226 Genomic DNA. Translation: AAA23873.1.
S58224 Genomic DNA. Translation: AAB26162.1.
U18997 Genomic DNA. Translation: AAA58228.1.
U00096 Genomic DNA. Translation: AAC76455.1.
AP009048 Genomic DNA. Translation: BAE77862.1.
PIRiA00721. YUEC.
RefSeqiNP_417888.1. NC_000913.3.
WP_000253975.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A6V1.
SMRiP0A6V1. Positions 16-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262502. 259 interactions.
DIPiDIP-48147N.
IntActiP0A6V1. 6 interactions.
STRINGi511145.b3430.

Proteomic databases

EPDiP0A6V1.
PaxDbiP0A6V1.
PRIDEiP0A6V1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76455; AAC76455; b3430.
BAE77862; BAE77862; BAE77862.
GeneIDi947942.
KEGGiecj:JW3393.
eco:b3430.
PATRICi32122300. VBIEscCol129921_3527.

Organism-specific databases

EchoBASEiEB0374.
EcoGeneiEG10379. glgC.

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
InParanoidiP0A6V1.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP0A6V1.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciEcoCyc:GLUC1PADENYLTRANS-MONOMER.
ECOL316407:JW3393-MONOMER.
MetaCyc:GLUC1PADENYLTRANS-MONOMER.
BRENDAi2.7.7.27. 2026.

Miscellaneous databases

PROiP0A6V1.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLGC_ECOLI
AccessioniPrimary (citable) accession number: P0A6V1
Secondary accession number(s): P00584, Q2M794
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.