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Protein

Glucose-1-phosphate adenylyltransferase

Gene

glgC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans.

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.

Enzyme regulationi

Activated by fructose-1,6-bisphosphate and inhibited by AMP and ADP. Allosteric regulation.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Allosteric activator1
Binding sitei114Glucose-1-phosphate1
Binding sitei195Glucose-1-phosphate1

GO - Molecular functioni

  • AMP binding Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • glucose-1-phosphate adenylyltransferase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • glycogen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLUC1PADENYLTRANS-MONOMER.
ECOL316407:JW3393-MONOMER.
MetaCyc:GLUC1PADENYLTRANS-MONOMER.
BRENDAi2.7.7.27. 2026.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferase (EC:2.7.7.27)
Alternative name(s):
ADP-glucose pyrophosphorylase
Short name:
ADPGlc PPase
ADP-glucose synthase
Gene namesi
Name:glgC
Ordered Locus Names:b3430, JW3393
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10379. glgC.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi195K → E, I, H, Q or R: No loss in enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001952942 – 431Glucose-1-phosphate adenylyltransferaseAdd BLAST430

Proteomic databases

EPDiP0A6V1.
PaxDbiP0A6V1.
PRIDEiP0A6V1.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4262502. 259 interactors.
DIPiDIP-48147N.
IntActiP0A6V1. 6 interactors.
STRINGi511145.b3430.

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 18Combined sources7
Beta strandi20 – 25Combined sources6
Helixi34 – 37Combined sources4
Beta strandi38 – 40Combined sources3
Helixi42 – 44Combined sources3
Turni48 – 50Combined sources3
Helixi54 – 63Combined sources10
Beta strandi68 – 75Combined sources8
Helixi78 – 87Combined sources10
Helixi93 – 95Combined sources3
Beta strandi98 – 103Combined sources6
Beta strandi108 – 110Combined sources3
Helixi117 – 123Combined sources7
Helixi125 – 130Combined sources6
Beta strandi134 – 140Combined sources7
Helixi149 – 158Combined sources10
Beta strandi162 – 171Combined sources10
Helixi172 – 177Combined sources6
Beta strandi178 – 183Combined sources6
Beta strandi187 – 195Combined sources9
Beta strandi208 – 219Combined sources12
Helixi220 – 232Combined sources13
Beta strandi233 – 235Combined sources3
Turni240 – 243Combined sources4
Helixi244 – 250Combined sources7
Beta strandi254 – 258Combined sources5
Helixi259 – 261Combined sources3
Helixi280 – 289Combined sources10
Beta strandi292 – 294Combined sources3
Beta strandi316 – 319Combined sources4
Beta strandi327 – 334Combined sources8
Beta strandi339 – 342Combined sources4
Beta strandi344 – 347Combined sources4
Beta strandi361 – 367Combined sources7
Beta strandi378 – 384Combined sources7
Beta strandi395 – 398Combined sources4
Helixi400 – 406Combined sources7
Beta strandi407 – 409Combined sources3
Beta strandi415 – 417Combined sources3
Helixi419 – 424Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5L6SX-ray3.04A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-431[»]
5L6VX-ray2.67A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-431[»]
ProteinModelPortaliP0A6V1.
SMRiP0A6V1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
InParanoidiP0A6V1.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP0A6V1.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLEKNDHL MLARQLPLKS VALILAGGRG TRLKDLTNKR AKPAVHFGGK
60 70 80 90 100
FRIIDFALSN CINSGIRRMG VITQYQSHTL VQHIQRGWSF FNEEMNEFVD
110 120 130 140 150
LLPAQQRMKG ENWYRGTADA VTQNLDIIRR YKAEYVVILA GDHIYKQDYS
160 170 180 190 200
RMLIDHVEKG ARCTVACMPV PIEEASAFGV MAVDENDKII EFVEKPANPP
210 220 230 240 250
SMPNDPSKSL ASMGIYVFDA DYLYELLEED DRDENSSHDF GKDLIPKITE
260 270 280 290 300
AGLAYAHPFP LSCVQSDPDA EPYWRDVGTL EAYWKANLDL ASVVPELDMY
310 320 330 340 350
DRNWPIRTYN ESLPPAKFVQ DRSGSHGMTL NSLVSGGCVI SGSVVVQSVL
360 370 380 390 400
FSRVRVNSFC NIDSAVLLPE VWVGRSCRLR RCVIDRACVI PEGMVIGENA
410 420 430
EEDARRFYRS EEGIVLVTRE MLRKLGHKQE R
Length:431
Mass (Da):48,698
Last modified:January 23, 2007 - v2
Checksum:i3C0A4C4F5B13D9D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161A → V in CAA23544 (PubMed:1320612).Curated1
Sequence conflicti161A → V in AAA98736 (PubMed:1320612).Curated1
Sequence conflicti161A → V in AAA23873 (PubMed:1320612).Curated1
Sequence conflicti166A → V in CAA23544 (PubMed:1320612).Curated1
Sequence conflicti166A → V in AAA98736 (PubMed:1320612).Curated1
Sequence conflicti166A → V in AAA23873 (PubMed:1320612).Curated1
Sequence conflicti189I → T in CAA23544 (PubMed:1320612).Curated1
Sequence conflicti189I → T in AAA98736 (PubMed:1320612).Curated1
Sequence conflicti189I → T in AAA23873 (PubMed:1320612).Curated1
Sequence conflicti296E → K in CAA23544 (PubMed:1320612).Curated1
Sequence conflicti296E → K in AAA98736 (PubMed:1320612).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti44A → T in SG14 mutant; lower apparent affinities for substrates, the activator, F16BP and the inhibitor. 1
Natural varianti67R → C in CL1136 mutant; less dependent on the allosteric activator, F16BP, for activity and less sensitive to inhibition by AMP. 1
Natural varianti295P → S in SG5 mutant. 1
Natural varianti336G → D in 618 mutant; causes lowered affinity for AMP. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00281 Genomic DNA. Translation: CAA23544.1.
J01616 Genomic DNA. Translation: AAA98736.1.
M97226 Genomic DNA. Translation: AAA23873.1.
S58224 Genomic DNA. Translation: AAB26162.1.
U18997 Genomic DNA. Translation: AAA58228.1.
U00096 Genomic DNA. Translation: AAC76455.1.
AP009048 Genomic DNA. Translation: BAE77862.1.
PIRiA00721. YUEC.
RefSeqiNP_417888.1. NC_000913.3.
WP_000253975.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76455; AAC76455; b3430.
BAE77862; BAE77862; BAE77862.
GeneIDi947942.
KEGGiecj:JW3393.
eco:b3430.
PATRICi32122300. VBIEscCol129921_3527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00281 Genomic DNA. Translation: CAA23544.1.
J01616 Genomic DNA. Translation: AAA98736.1.
M97226 Genomic DNA. Translation: AAA23873.1.
S58224 Genomic DNA. Translation: AAB26162.1.
U18997 Genomic DNA. Translation: AAA58228.1.
U00096 Genomic DNA. Translation: AAC76455.1.
AP009048 Genomic DNA. Translation: BAE77862.1.
PIRiA00721. YUEC.
RefSeqiNP_417888.1. NC_000913.3.
WP_000253975.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5L6SX-ray3.04A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-431[»]
5L6VX-ray2.67A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-431[»]
ProteinModelPortaliP0A6V1.
SMRiP0A6V1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262502. 259 interactors.
DIPiDIP-48147N.
IntActiP0A6V1. 6 interactors.
STRINGi511145.b3430.

Proteomic databases

EPDiP0A6V1.
PaxDbiP0A6V1.
PRIDEiP0A6V1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76455; AAC76455; b3430.
BAE77862; BAE77862; BAE77862.
GeneIDi947942.
KEGGiecj:JW3393.
eco:b3430.
PATRICi32122300. VBIEscCol129921_3527.

Organism-specific databases

EchoBASEiEB0374.
EcoGeneiEG10379. glgC.

Phylogenomic databases

eggNOGiENOG4107QQ4. Bacteria.
COG0448. LUCA.
HOGENOMiHOG000278607.
InParanoidiP0A6V1.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP0A6V1.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciEcoCyc:GLUC1PADENYLTRANS-MONOMER.
ECOL316407:JW3393-MONOMER.
MetaCyc:GLUC1PADENYLTRANS-MONOMER.
BRENDAi2.7.7.27. 2026.

Miscellaneous databases

PROiP0A6V1.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
HAMAPiMF_00624. GlgC. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR023049. GlgC_bac.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLGC_ECOLI
AccessioniPrimary (citable) accession number: P0A6V1
Secondary accession number(s): P00584, Q2M794
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.