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P0A6U8 (GLGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogen synthase
EC=2.4.1.21
Alternative name(s):
Starch [bacterial glycogen] synthase
Gene names
Name:glgA
Ordered Locus Names:b3429, JW3392
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Synthesizes alpha-1,4-glucan chains using ADP-glucose. HAMAP-Rule MF_00484

Catalytic activity

ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1). HAMAP-Rule MF_00484

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00484

Sequence similarities

Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-1132777,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Glycogen synthase HAMAP-Rule MF_00484
PRO_0000188611

Sites

Binding site151ADP-glucose

Experimental info

Sequence conflict631F → S in AAA23870. Ref.1
Sequence conflict102 – 1032AY → VH in AAA23870. Ref.1
Sequence conflict2451L → P in AAA23870. Ref.1
Sequence conflict2801L → S in AAA23870. Ref.1
Sequence conflict2861L → S in AAA23870. Ref.1
Sequence conflict3091L → S in AAA23870. Ref.1
Sequence conflict3141L → S in AAA23870. Ref.1
Sequence conflict317 – 3182LL → SS in AAA23870. Ref.1
Sequence conflict3871L → S in AAA23870. Ref.1
Sequence conflict4191S → N in AAA23870. Ref.1
Sequence conflict4221V → I in AAA23870. Ref.1
Sequence conflict4341A → T in AAA23870. Ref.1
Sequence conflict4441R → C Ref.1
Sequence conflict4461S → P Ref.1
Sequence conflict4611S → G in AAA23870. Ref.1
Sequence conflict4761L → S in AAA23870. Ref.1

Secondary structure

1 ................................................................................................ 477
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A6U8 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: 177CF37F8C97CF42

FASTA47752,822
        10         20         30         40         50         60 
MQVLHVCSEM FPLLKTGGLA DVIGALPAAQ IADGVDARVL LPAFPDIRRG VTDAQVVSRR 

        70         80         90        100        110        120 
DTFAGHITLL FGHYNGVGIY LIDAPHLYDR PGSPYHDTNL FAYTDNVLRF ALLGWVGAEM 

       130        140        150        160        170        180 
ASGLDPFWRP DVVHAHDWHA GLAPAYLAAR GRPAKSVFTV HNLAYQGMFY AHHMNDIQLP 

       190        200        210        220        230        240 
WSFFNIHGLE FNGQISFLKA GLYYADHITA VSPTYAREIT EPQFAYGMEG LLQQRHREGR 

       250        260        270        280        290        300 
LSGVLNGVDE KIWSPETDLL LASRYTRDTL EDKAENKRQL QIAMGLKVDD KVPLFAVVSR 

       310        320        330        340        350        360 
LTSQKGLDLV LEALPGLLEQ GGQLALLGAG DPVLQEGFLA AAAEYPGQVG VQIGYHEAFS 

       370        380        390        400        410        420 
HRIMGGADVI LVPSRFEPCG LTQLYGLKYG TLPLVRRTGG LADTVSDCSL ENLADGVASG 

       430        440        450        460        470 
FVFEDSNAWS LLRAIRRAFV LWSRPSLWRF VQRQAMAMDF SWQVAAKSYR ELYYRLK

« Hide

References

« Hide 'large scale' references
[1]"Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose:alpha-1,4-glucan, 4-glucosyltransferase as deduced from the nucleotide sequence of the glgA gene."
Kumar A., Larsen C.E., Preiss J.
J. Biol. Chem. 261:16256-16259(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose synthetase as deduced from the nucleotide sequence of the glg C gene."
Baecker P.A., Furlong C.E., Preiss J.
J. Biol. Chem. 258:5084-5088(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
[5]"The 55-kilodalton protein in an oriC complex fraction is glycogen synthase."
Kaidow A., Kataoka T., Wachi M., Takada A., Yamasaki M., Nagai K.
J. Bacteriol. 174:5454-5456(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		V00281 Genomic DNA. Translation: CAA23545.1.
J02616 Genomic DNA. Translation: AAA23870.1.
U18997 Genomic DNA. Translation: AAA58227.1.
U00096 Genomic DNA. Translation: AAC76454.1.
AP009048 Genomic DNA. Translation: BAE77863.1.
J01616 Genomic DNA. Translation: AAA98737.1.
PIRSYECGL. H65138.
RefSeqNP_417887.1. NC_000913.2.
YP_492004.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QZSX-ray2.20A1-477[»]
2R4TX-ray2.26A1-477[»]
2R4UX-ray2.37A1-477[»]
3COPX-ray2.30A1-477[»]
3CX4X-ray2.29A1-477[»]
3D1JX-ray3.00A1-477[»]
3GUHX-ray2.79A1-477[»]
ProteinModelPortalP0A6U8.
SMRP0A6U8. Positions 1-477.
ModBaseSearch...

Protein-protein interaction databases

IntActP0A6U8. 1 interaction.
STRING511145.b3429.

Proteomic databases

PaxDbP0A6U8.
PRIDEP0A6U8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76454; AAC76454; b3429.
BAE77863; BAE77863; BAE77863.
GeneID12933507.
947932.
KEGGecj:Y75_p3748.
eco:b3429.
PATRIC32122298. VBIEscCol129921_3526.

Organism-specific databases

EchoBASEEB0372.
EcoGeneEG10377. glgA.

Phylogenomic databases

eggNOGCOG0297.
HOGENOMHOG000294941.
KOK00703.
OMAADVIMVP.
ProtClustDBPRK00654.

Enzyme and pathway databases

BioCycEcoCyc:GLYCOGENSYN-MONOMER.
ECOL316407:JW3392-MONOMER.
MetaCyc:GLYCOGENSYN-MONOMER.
UniPathwayUPA00164.

Gene expression databases

GenevestigatorP0A6U8.

Family and domain databases

HAMAPMF_00484. Glycogen_synth.
InterProIPR001296. Glyco_trans_1.
IPR011835. Glycogen/starch_synth.
IPR013534. Starch_synth_cat_dom.
[Graphical view]
PfamPF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR02095. glgA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A6U8.

Entry information

Entry nameGLGA_ECOLI
AccessionPrimary (citable) accession number: P0A6U8
Secondary accession number(s): P08323, Q2M793
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: March 29, 2005
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents