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P0A6U8

- GLGA_ECOLI

UniProt

P0A6U8 - GLGA_ECOLI

Protein

Glycogen synthase

Gene

glgA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Synthesizes alpha-1,4-glucan chains using ADP-glucose.

    Catalytic activityi

    ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ADP-glucose

    GO - Molecular functioni

    1. starch synthase activity Source: EcoCyc

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. glycogen biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:GLYCOGENSYN-MONOMER.
    ECOL316407:JW3392-MONOMER.
    MetaCyc:GLYCOGENSYN-MONOMER.
    UniPathwayiUPA00164.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen synthase (EC:2.4.1.21)
    Alternative name(s):
    Starch [bacterial glycogen] synthase
    Gene namesi
    Name:glgA
    Ordered Locus Names:b3429, JW3392
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10377. glgA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Glycogen synthasePRO_0000188611Add
    BLAST

    Proteomic databases

    PaxDbiP0A6U8.
    PRIDEiP0A6U8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6U8.

    Interactioni

    Protein-protein interaction databases

    IntActiP0A6U8. 1 interaction.
    STRINGi511145.b3429.

    Structurei

    Secondary structure

    1
    477
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Turni11 – 133
    Helixi18 – 3215
    Beta strandi36 – 427
    Helixi45 – 506
    Beta strandi55 – 606
    Beta strandi67 – 748
    Beta strandi77 – 837
    Helixi85 – 884
    Beta strandi91 – 933
    Beta strandi94 – 963
    Helixi106 – 12015
    Turni121 – 1233
    Beta strandi131 – 1366
    Helixi138 – 1403
    Helixi143 – 1497
    Beta strandi153 – 1619
    Beta strandi168 – 1703
    Helixi171 – 1755
    Turni176 – 1783
    Helixi181 – 1833
    Turni186 – 1894
    Beta strandi194 – 1963
    Helixi197 – 2048
    Beta strandi206 – 2127
    Helixi213 – 2186
    Helixi222 – 2254
    Helixi229 – 2379
    Beta strandi241 – 2433
    Turni250 – 2523
    Turni255 – 2573
    Beta strandi261 – 2633
    Helixi270 – 2734
    Helixi274 – 28411
    Beta strandi290 – 2923
    Beta strandi294 – 3029
    Helixi303 – 3053
    Helixi307 – 31913
    Beta strandi323 – 3308
    Helixi332 – 34413
    Turni346 – 3483
    Beta strandi349 – 3546
    Helixi357 – 36610
    Beta strandi368 – 3725
    Helixi382 – 3898
    Beta strandi392 – 3987
    Helixi399 – 4046
    Helixi410 – 4145
    Beta strandi420 – 4234
    Beta strandi425 – 4273
    Helixi428 – 44215
    Helixi445 – 45713
    Helixi462 – 47615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QZSX-ray2.20A1-477[»]
    2R4TX-ray2.26A1-477[»]
    2R4UX-ray2.37A1-477[»]
    3COPX-ray2.30A1-477[»]
    3CX4X-ray2.29A1-477[»]
    3D1JX-ray3.00A1-477[»]
    3GUHX-ray2.79A1-477[»]
    ProteinModelPortaliP0A6U8.
    SMRiP0A6U8. Positions 1-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A6U8.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0297.
    HOGENOMiHOG000294941.
    KOiK00703.
    OMAiPYHDESQ.
    OrthoDBiEOG6JTC6Z.
    PhylomeDBiP0A6U8.

    Family and domain databases

    HAMAPiMF_00484. Glycogen_synth.
    InterProiIPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view]
    PfamiPF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02095. glgA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6U8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVLHVCSEM FPLLKTGGLA DVIGALPAAQ IADGVDARVL LPAFPDIRRG    50
    VTDAQVVSRR DTFAGHITLL FGHYNGVGIY LIDAPHLYDR PGSPYHDTNL 100
    FAYTDNVLRF ALLGWVGAEM ASGLDPFWRP DVVHAHDWHA GLAPAYLAAR 150
    GRPAKSVFTV HNLAYQGMFY AHHMNDIQLP WSFFNIHGLE FNGQISFLKA 200
    GLYYADHITA VSPTYAREIT EPQFAYGMEG LLQQRHREGR LSGVLNGVDE 250
    KIWSPETDLL LASRYTRDTL EDKAENKRQL QIAMGLKVDD KVPLFAVVSR 300
    LTSQKGLDLV LEALPGLLEQ GGQLALLGAG DPVLQEGFLA AAAEYPGQVG 350
    VQIGYHEAFS HRIMGGADVI LVPSRFEPCG LTQLYGLKYG TLPLVRRTGG 400
    LADTVSDCSL ENLADGVASG FVFEDSNAWS LLRAIRRAFV LWSRPSLWRF 450
    VQRQAMAMDF SWQVAAKSYR ELYYRLK 477
    Length:477
    Mass (Da):52,822
    Last modified:March 29, 2005 - v1
    Checksum:i177CF37F8C97CF42
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631F → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti102 – 1032AY → VH in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti245 – 2451L → P in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti280 – 2801L → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti286 – 2861L → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti309 – 3091L → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti314 – 3141L → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti317 – 3182LL → SS in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti387 – 3871L → S in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti419 – 4191S → N in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti422 – 4221V → I in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti434 – 4341A → T in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti444 – 4441R → C(PubMed:3097003)Curated
    Sequence conflicti446 – 4461S → P(PubMed:3097003)Curated
    Sequence conflicti461 – 4611S → G in AAA23870. (PubMed:3097003)Curated
    Sequence conflicti476 – 4761L → S in AAA23870. (PubMed:3097003)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00281 Genomic DNA. Translation: CAA23545.1.
    J02616 Genomic DNA. Translation: AAA23870.1.
    U18997 Genomic DNA. Translation: AAA58227.1.
    U00096 Genomic DNA. Translation: AAC76454.1.
    AP009048 Genomic DNA. Translation: BAE77863.1.
    J01616 Genomic DNA. Translation: AAA98737.1.
    PIRiH65138. SYECGL.
    RefSeqiNP_417887.1. NC_000913.3.
    YP_492004.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76454; AAC76454; b3429.
    BAE77863; BAE77863; BAE77863.
    GeneIDi12933507.
    947932.
    KEGGiecj:Y75_p3748.
    eco:b3429.
    PATRICi32122298. VBIEscCol129921_3526.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00281 Genomic DNA. Translation: CAA23545.1 .
    J02616 Genomic DNA. Translation: AAA23870.1 .
    U18997 Genomic DNA. Translation: AAA58227.1 .
    U00096 Genomic DNA. Translation: AAC76454.1 .
    AP009048 Genomic DNA. Translation: BAE77863.1 .
    J01616 Genomic DNA. Translation: AAA98737.1 .
    PIRi H65138. SYECGL.
    RefSeqi NP_417887.1. NC_000913.3.
    YP_492004.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QZS X-ray 2.20 A 1-477 [» ]
    2R4T X-ray 2.26 A 1-477 [» ]
    2R4U X-ray 2.37 A 1-477 [» ]
    3COP X-ray 2.30 A 1-477 [» ]
    3CX4 X-ray 2.29 A 1-477 [» ]
    3D1J X-ray 3.00 A 1-477 [» ]
    3GUH X-ray 2.79 A 1-477 [» ]
    ProteinModelPortali P0A6U8.
    SMRi P0A6U8. Positions 1-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A6U8. 1 interaction.
    STRINGi 511145.b3429.

    Proteomic databases

    PaxDbi P0A6U8.
    PRIDEi P0A6U8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76454 ; AAC76454 ; b3429 .
    BAE77863 ; BAE77863 ; BAE77863 .
    GeneIDi 12933507.
    947932.
    KEGGi ecj:Y75_p3748.
    eco:b3429.
    PATRICi 32122298. VBIEscCol129921_3526.

    Organism-specific databases

    EchoBASEi EB0372.
    EcoGenei EG10377. glgA.

    Phylogenomic databases

    eggNOGi COG0297.
    HOGENOMi HOG000294941.
    KOi K00703.
    OMAi PYHDESQ.
    OrthoDBi EOG6JTC6Z.
    PhylomeDBi P0A6U8.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci EcoCyc:GLYCOGENSYN-MONOMER.
    ECOL316407:JW3392-MONOMER.
    MetaCyc:GLYCOGENSYN-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A6U8.
    PROi P0A6U8.

    Gene expression databases

    Genevestigatori P0A6U8.

    Family and domain databases

    HAMAPi MF_00484. Glycogen_synth.
    InterProi IPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view ]
    Pfami PF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02095. glgA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose:alpha-1,4-glucan, 4-glucosyltransferase as deduced from the nucleotide sequence of the glgA gene."
      Kumar A., Larsen C.E., Preiss J.
      J. Biol. Chem. 261:16256-16259(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose synthetase as deduced from the nucleotide sequence of the glg C gene."
      Baecker P.A., Furlong C.E., Preiss J.
      J. Biol. Chem. 258:5084-5088(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    5. "The 55-kilodalton protein in an oriC complex fraction is glycogen synthase."
      Kaidow A., Kataoka T., Wachi M., Takada A., Yamasaki M., Nagai K.
      J. Bacteriol. 174:5454-5456(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10.

    Entry informationi

    Entry nameiGLGA_ECOLI
    AccessioniPrimary (citable) accession number: P0A6U8
    Secondary accession number(s): P08323, Q2M793
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3