Glycogen synthase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A6U8 (GLGA_ECOLI)

Last modified June 16, 2009. Version 30. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glycogen synthase
    EC=2.4.1.21
Alternative name(s):
    Starch [bacterial glycogen] synthase

Gene names

Name:	glgA
Ordered Locus Names:	b3429, JW3392

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Synthesizes alpha-1,4-glucan chains using ADP-glucose. HAMAP MF_00484
Catalytic activity	ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1). HAMAP MF_00484
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00484
Sequence similarities	Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

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Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	protein binding Inferred from physical interaction. Source: IntAct starch synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1132777,EBI-543750

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 477

477

Glycogen synthase HAMAP MF_00484

PRO_0000188611

Sites

Binding site

ADP-glucose HAMAP MF_00484

Experimental info

Sequence conflict

F → S in AAA23870. Ref.1

Sequence conflict

102 – 103

AY → VH in AAA23870. Ref.1

Sequence conflict

245

L → P in AAA23870. Ref.1

Sequence conflict

280

L → S in AAA23870. Ref.1

Sequence conflict

286

L → S in AAA23870. Ref.1

Sequence conflict

309

L → S in AAA23870. Ref.1

Sequence conflict

314

L → S in AAA23870. Ref.1

Sequence conflict

317 – 318

LL → SS in AAA23870. Ref.1

Sequence conflict

387

L → S in AAA23870. Ref.1

Sequence conflict

419

S → N in AAA23870. Ref.1

Sequence conflict

422

V → I in AAA23870. Ref.1

Sequence conflict

434

A → T in AAA23870. Ref.1

Sequence conflict

444

R → C Ref.1

Sequence conflict

446

S → P Ref.1

Sequence conflict

461

S → G in AAA23870. Ref.1

Sequence conflict

476

L → S in AAA23870. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A6U8-1 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: 177CF37F8C97CF42
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FASTA

477

52,822

        10         20         30         40         50         60 
MQVLHVCSEM FPLLKTGGLA DVIGALPAAQ IADGVDARVL LPAFPDIRRG VTDAQVVSRR 

        70         80         90        100        110        120 
DTFAGHITLL FGHYNGVGIY LIDAPHLYDR PGSPYHDTNL FAYTDNVLRF ALLGWVGAEM 

       130        140        150        160        170        180 
ASGLDPFWRP DVVHAHDWHA GLAPAYLAAR GRPAKSVFTV HNLAYQGMFY AHHMNDIQLP 

       190        200        210        220        230        240 
WSFFNIHGLE FNGQISFLKA GLYYADHITA VSPTYAREIT EPQFAYGMEG LLQQRHREGR 

       250        260        270        280        290        300 
LSGVLNGVDE KIWSPETDLL LASRYTRDTL EDKAENKRQL QIAMGLKVDD KVPLFAVVSR 

       310        320        330        340        350        360 
LTSQKGLDLV LEALPGLLEQ GGQLALLGAG DPVLQEGFLA AAAEYPGQVG VQIGYHEAFS 

       370        380        390        400        410        420 
HRIMGGADVI LVPSRFEPCG LTQLYGLKYG TLPLVRRTGG LADTVSDCSL ENLADGVASG 

       430        440        450        460        470 
FVFEDSNAWS LLRAIRRAFV LWSRPSLWRF VQRQAMAMDF SWQVAAKSYR ELYYRLK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose:alpha-1,4-glucan, 4-glucosyltransferase as deduced from the nucleotide sequence of the glgA gene." Kumar A., Larsen C.E., Preiss J. J. Biol. Chem. 261:16256-16259(1986) [PubMed: 3097003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose synthetase as deduced from the nucleotide sequence of the glg C gene." Baecker P.A., Furlong C.E., Preiss J. J. Biol. Chem. 258:5084-5088(1983) [PubMed: 6300111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
[5]	"The 55-kilodalton protein in an oriC complex fraction is glycogen synthase." Kaidow A., Kataoka T., Wachi M., Takada A., Yamasaki M., Nagai K. J. Bacteriol. 174:5454-5456(1992) [PubMed: 1644773] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

V00281 Genomic DNA. Translation: CAA23545.1.
J02616 Genomic DNA. Translation: AAA23870.1.
U18997 Genomic DNA. Translation: AAA58227.1.
U00096 Genomic DNA. Translation: AAC76454.1.
AP009048 Genomic DNA. Translation: BAE77863.1.
J01616 Genomic DNA. Translation: AAA98737.1.

PIR

SYECGL. H65138.

RefSeq

AP_004362.1.
NP_417887.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QZS	X-ray	2.20	A	1-477	[»]
2R4T	X-ray	2.26	A	1-477	[»]
2R4U	X-ray	2.37	A	1-477	[»]
3COP	X-ray	2.30	A	1-477	[»]
3CX4	X-ray	2.29	A	1-477	[»]
3D1J	X-ray	3.00	A	1-477	[»]
3GUH	X-ray	2.79	A	1-477	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A6U8. 1 interaction.

Genome annotation databases

GeneID

947932.

GenomeReviews

Gene locus JW3392 in contig AP009048_GR.
Gene locus b3429 in contig U00096_GR.

KEGG

ecj:JW3392.
eco:b3429.

Organism-specific databases

EchoBASE

EB0372.

EcoGene

EG10377. glgA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A6U8.

OMA

P0A6U8. YQGPWRW.

Enzyme and pathway databases

BioCyc

EcoCyc:GLYCOGENSYN-MON.
MetaCyc:GLYCOGENSYN-MON.

Family and domain databases

HAMAP

MF_00484.
[Tree]

InterPro

IPR011835. GlgA.
IPR001296. Glyco_trans_1.
IPR013534. Glyco_transf_5.
[Graphical view]

Pfam

PF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02095. glgA. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

GLGA_ECOLI

Accession

Primary (citable) accession number: P0A6U8
Secondary accession number(s): P08323, Q2M793

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	March 29, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents