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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Requires the intact 30S subunit for methylation.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei139S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • rRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER.
ECOL316407:JW3718-MONOMER.
MetaCyc:EG10376-MONOMER.
BRENDAi2.1.1.170. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:b3740, JW3718
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10376. rsmG.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39N → A: Lack of activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi53H → A: Decrease in activity. Very low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi56D → A: Strong decrease in activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi71D → A: Lack of activity. Low-level streptomycin resistance. 10-fold increase in AdoMet affinity. 1 Publication1
Mutagenesisi73G → A: Slight decrease in activity. Streptomycin-sensitive. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi75G → A: Lack of activity. Low-level streptomycin resistance. 24-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi77G → A: Lack of activity. Low-level streptomycin resistance. 10-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi79P → A: Lack of activity. Low-level streptomycin resistance. 11-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi96D → A: Lack of activity. Low-level streptomycin resistance. 1 Publication1
Mutagenesisi100 – 101KR → AA: Lack of activity. Low-level streptomycin resistance. 1 Publication2
Mutagenesisi123R → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication1
Mutagenesisi139R → A: Lack of activity. Low-level streptomycin resistance. No change in AdoMet affinity. 1 Publication1
Mutagenesisi139R → K: Decrease in activity. Very low-level streptomycin resistance. 1 Publication1
Mutagenesisi165K → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication1
Mutagenesisi197R → A: Strong decrease in activity. Low-level streptomycin resistance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001842491 – 207Ribosomal RNA small subunit methyltransferase GAdd BLAST207

Proteomic databases

EPDiP0A6U5.
PaxDbiP0A6U5.
PRIDEiP0A6U5.

Expressioni

Inductioni

Induced under stress conditions known to stimulate ppGpp accumulation and down-regulate rRNA synthesis.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263261. 246 interactors.
DIPiDIP-47992N.
IntActiP0A6U5. 10 interactors.
STRINGi511145.b3740.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 10Combined sources9
Turni11 – 13Combined sources3
Helixi18 – 34Combined sources17
Helixi49 – 60Combined sources12
Helixi61 – 63Combined sources3
Beta strandi66 – 72Combined sources7
Turni75 – 79Combined sources5
Helixi80 – 86Combined sources7
Beta strandi90 – 97Combined sources8
Helixi99 – 111Combined sources13
Beta strandi115 – 121Combined sources7
Turni124 – 126Combined sources3
Beta strandi133 – 137Combined sources5
Beta strandi141 – 143Combined sources3
Helixi144 – 151Combined sources8
Beta strandi154 – 167Combined sources14
Helixi170 – 174Combined sources5
Beta strandi180 – 189Combined sources10
Beta strandi196 – 204Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JSXX-ray2.40A1-207[»]
ProteinModelPortaliP0A6U5.
SMRiP0A6U5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6U5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiP0A6U5.
KOiK03501.
OMAiKWNGVYN.
PhylomeDBiP0A6U5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKLSLLLK DAGISLTDHQ KNQLIAYVNM LHKWNKAYNL TSVRDPNEML
60 70 80 90 100
VRHILDSIVV APYLQGERFI DVGTGPGLPG IPLSIVRPEA HFTLLDSLGK
110 120 130 140 150
RVRFLRQVQH ELKLENIEPV QSRVEEFPSE PPFDGVISRA FASLNDMVSW
160 170 180 190 200
CHHLPGEQGR FYALKGQMPE DEIALLPEEY QVESVVKLQV PALDGERHLV

VIKANKI
Length:207
Mass (Da):23,431
Last modified:March 29, 2005 - v1
Checksum:i40CF9BA134E9A234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25774.1.
L10328 Genomic DNA. Translation: AAA62092.1.
U00096 Genomic DNA. Translation: AAC76763.1.
AP009048 Genomic DNA. Translation: BAE77548.1.
X01383 Genomic DNA. Translation: CAA25639.1.
PIRiC30389. BVECQB.
RefSeqiNP_418196.1. NC_000913.3.
WP_000932839.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740.
BAE77548; BAE77548; BAE77548.
GeneIDi948250.
KEGGiecj:JW3718.
eco:b3740.
PATRICi32122979. VBIEscCol129921_3865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25774.1.
L10328 Genomic DNA. Translation: AAA62092.1.
U00096 Genomic DNA. Translation: AAC76763.1.
AP009048 Genomic DNA. Translation: BAE77548.1.
X01383 Genomic DNA. Translation: CAA25639.1.
PIRiC30389. BVECQB.
RefSeqiNP_418196.1. NC_000913.3.
WP_000932839.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JSXX-ray2.40A1-207[»]
ProteinModelPortaliP0A6U5.
SMRiP0A6U5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263261. 246 interactors.
DIPiDIP-47992N.
IntActiP0A6U5. 10 interactors.
STRINGi511145.b3740.

Proteomic databases

EPDiP0A6U5.
PaxDbiP0A6U5.
PRIDEiP0A6U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740.
BAE77548; BAE77548; BAE77548.
GeneIDi948250.
KEGGiecj:JW3718.
eco:b3740.
PATRICi32122979. VBIEscCol129921_3865.

Organism-specific databases

EchoBASEiEB0371.
EcoGeneiEG10376. rsmG.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiP0A6U5.
KOiK03501.
OMAiKWNGVYN.
PhylomeDBiP0A6U5.

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER.
ECOL316407:JW3718-MONOMER.
MetaCyc:EG10376-MONOMER.
BRENDAi2.1.1.170. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A6U5.
PROiP0A6U5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMG_ECOLI
AccessioniPrimary (citable) accession number: P0A6U5
Secondary accession number(s): P17113, Q2M858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mechanisms acting on transcription regulation, translation or protein stability could be responsible for adjusting RsmG expression to rRNA synthesis.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.