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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Requires the intact 30S subunit for methylation.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei78 – 781S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei139 – 1391S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  • rRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER.
ECOL316407:JW3718-MONOMER.
MetaCyc:EG10376-MONOMER.
BRENDAi2.1.1.170. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:b3740, JW3718
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10376. rsmG.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391N → A: Lack of activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication
Mutagenesisi53 – 531H → A: Decrease in activity. Very low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication
Mutagenesisi56 – 561D → A: Strong decrease in activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication
Mutagenesisi71 – 711D → A: Lack of activity. Low-level streptomycin resistance. 10-fold increase in AdoMet affinity. 1 Publication
Mutagenesisi73 – 731G → A: Slight decrease in activity. Streptomycin-sensitive. Slight decrease in AdoMet affinity. 1 Publication
Mutagenesisi75 – 751G → A: Lack of activity. Low-level streptomycin resistance. 24-fold decrease in AdoMet affinity. 1 Publication
Mutagenesisi77 – 771G → A: Lack of activity. Low-level streptomycin resistance. 10-fold decrease in AdoMet affinity. 1 Publication
Mutagenesisi79 – 791P → A: Lack of activity. Low-level streptomycin resistance. 11-fold decrease in AdoMet affinity. 1 Publication
Mutagenesisi96 – 961D → A: Lack of activity. Low-level streptomycin resistance. 1 Publication
Mutagenesisi100 – 1012KR → AA: Lack of activity. Low-level streptomycin resistance. 1 Publication
Mutagenesisi123 – 1231R → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication
Mutagenesisi139 – 1391R → A: Lack of activity. Low-level streptomycin resistance. No change in AdoMet affinity. 1 Publication
Mutagenesisi139 – 1391R → K: Decrease in activity. Very low-level streptomycin resistance. 1 Publication
Mutagenesisi165 – 1651K → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication
Mutagenesisi197 – 1971R → A: Strong decrease in activity. Low-level streptomycin resistance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Ribosomal RNA small subunit methyltransferase GPRO_0000184249Add
BLAST

Proteomic databases

EPDiP0A6U5.
PaxDbiP0A6U5.

Expressioni

Inductioni

Induced under stress conditions known to stimulate ppGpp accumulation and down-regulate rRNA synthesis.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263261. 246 interactions.
DIPiDIP-47992N.
IntActiP0A6U5. 10 interactions.
STRINGi511145.b3740.

Structurei

Secondary structure

1
207
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 109Combined sources
Turni11 – 133Combined sources
Helixi18 – 3417Combined sources
Helixi49 – 6012Combined sources
Helixi61 – 633Combined sources
Beta strandi66 – 727Combined sources
Turni75 – 795Combined sources
Helixi80 – 867Combined sources
Beta strandi90 – 978Combined sources
Helixi99 – 11113Combined sources
Beta strandi115 – 1217Combined sources
Turni124 – 1263Combined sources
Beta strandi133 – 1375Combined sources
Beta strandi141 – 1433Combined sources
Helixi144 – 1518Combined sources
Beta strandi154 – 16714Combined sources
Helixi170 – 1745Combined sources
Beta strandi180 – 18910Combined sources
Beta strandi196 – 2049Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JSXX-ray2.40A1-207[»]
ProteinModelPortaliP0A6U5.
SMRiP0A6U5. Positions 1-207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6U5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiP0A6U5.
KOiK03501.
OMAiKWNGVYN.
OrthoDBiEOG6HF639.
PhylomeDBiP0A6U5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKLSLLLK DAGISLTDHQ KNQLIAYVNM LHKWNKAYNL TSVRDPNEML
60 70 80 90 100
VRHILDSIVV APYLQGERFI DVGTGPGLPG IPLSIVRPEA HFTLLDSLGK
110 120 130 140 150
RVRFLRQVQH ELKLENIEPV QSRVEEFPSE PPFDGVISRA FASLNDMVSW
160 170 180 190 200
CHHLPGEQGR FYALKGQMPE DEIALLPEEY QVESVVKLQV PALDGERHLV

VIKANKI
Length:207
Mass (Da):23,431
Last modified:March 29, 2005 - v1
Checksum:i40CF9BA134E9A234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25774.1.
L10328 Genomic DNA. Translation: AAA62092.1.
U00096 Genomic DNA. Translation: AAC76763.1.
AP009048 Genomic DNA. Translation: BAE77548.1.
X01383 Genomic DNA. Translation: CAA25639.1.
PIRiC30389. BVECQB.
RefSeqiNP_418196.1. NC_000913.3.
WP_000932839.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740.
BAE77548; BAE77548; BAE77548.
GeneIDi948250.
KEGGiecj:JW3718.
eco:b3740.
PATRICi32122979. VBIEscCol129921_3865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25774.1.
L10328 Genomic DNA. Translation: AAA62092.1.
U00096 Genomic DNA. Translation: AAC76763.1.
AP009048 Genomic DNA. Translation: BAE77548.1.
X01383 Genomic DNA. Translation: CAA25639.1.
PIRiC30389. BVECQB.
RefSeqiNP_418196.1. NC_000913.3.
WP_000932839.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JSXX-ray2.40A1-207[»]
ProteinModelPortaliP0A6U5.
SMRiP0A6U5. Positions 1-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263261. 246 interactions.
DIPiDIP-47992N.
IntActiP0A6U5. 10 interactions.
STRINGi511145.b3740.

Proteomic databases

EPDiP0A6U5.
PaxDbiP0A6U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740.
BAE77548; BAE77548; BAE77548.
GeneIDi948250.
KEGGiecj:JW3718.
eco:b3740.
PATRICi32122979. VBIEscCol129921_3865.

Organism-specific databases

EchoBASEiEB0371.
EcoGeneiEG10376. rsmG.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiP0A6U5.
KOiK03501.
OMAiKWNGVYN.
OrthoDBiEOG6HF639.
PhylomeDBiP0A6U5.

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER.
ECOL316407:JW3718-MONOMER.
MetaCyc:EG10376-MONOMER.
BRENDAi2.1.1.170. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A6U5.
PROiP0A6U5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS."
    Walker J.E., Gay N.J., Saraste M., Eberle A.N.
    Biochem. J. 224:799-815(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The promoters of the atp operon of Escherichia coli K12."
    Nielsen J., Joergensen B.B., von Meyenburg K., Hansen F.G.
    Mol. Gen. Genet. 193:64-71(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 192-207.
    Strain: K12.
  6. "Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria."
    Okamoto S., Tamaru A., Nakajima C., Nishimura K., Tanaka Y., Tokuyama S., Suzuki Y., Ochi K.
    Mol. Microbiol. 63:1096-1106(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    Strain: K12.
  7. "Regulation of expression and catalytic activity of Escherichia coli RsmG methyltransferase."
    Benitez-Paez A., Villarroya M., Armengod M.E.
    RNA 18:795-806(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, MUTAGENESIS OF HIS-53; ASP-56; ASP-71; GLY-73; GLY-75; GLY-77; PRO-79; ASP-96; 100-LYS-ARG-101; ARG-123; ARG-139; LYS-165 AND ARG-197.
  8. "Impairing methylations at ribosome RNA, a point mutation-dependent strategy for aminoglycoside resistance: the rsmG case."
    Benitez-Paez A., Cardenas-Brito S., Corredor M., Villarroya M., Armengod M.E.
    Biomedica 34:41-49(2014)
    Cited for: MUTAGENESIS OF ASN-39.
  9. "Crystal structure of the Escherichia coli glucose-inhibited division protein B (GidB) reveals a methyltransferase fold."
    Romanowski M.J., Bonanno J.B., Burley S.K.
    Proteins 47:563-567(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiRSMG_ECOLI
AccessioniPrimary (citable) accession number: P0A6U5
Secondary accession number(s): P17113, Q2M858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mechanisms acting on transcription regulation, translation or protein stability could be responsible for adjusting RsmG expression to rRNA synthesis.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.