P0A6U5 (RSMG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase
Glucose-inhibited division protein B

Gene names

Name:	rsmG
Synonyms:	gidB
Ordered Locus Names:	b3740, JW3718

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA. Ref.6
Catalytic activity	S-adenosyl-L-methionine + guanosine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanosine(527) in 16S rRNA. HAMAP MF_00074
Subcellular location	Cytoplasm Potential HAMAP MF_00074.
Disruption phenotype	Low-level streptomycin resistance. Ref.6
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N7-)-methyltransferase activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074

PRO_0000184249

Secondary structure

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207

Helix Strand Turn

Details...

Helix

2 – 10

9

Turn

11 – 13

3

Helix

18 – 34

17

Helix

49 – 60

12

Helix

61 – 63

3

Beta strand

66 – 72

7

Turn

75 – 79

5

Helix

80 – 86

7

Beta strand

90 – 97

8

Helix

99 – 111

13

Beta strand

115 – 121

7

Turn

124 – 126

3

Beta strand

133 – 137

5

Beta strand

141 – 143

3

Helix

144 – 151

8

Beta strand

154 – 167

14

Helix

170 – 174

5

Beta strand

180 – 189

10

Beta strand

196 – 204

9

Sequences

	Sequence		Length	Mass (Da)
	P0A6U5 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 40CF9BA134E9A234	FASTA	207	23,431
	10 20 30 40 50 60 MLNKLSLLLK DAGISLTDHQ KNQLIAYVNM LHKWNKAYNL TSVRDPNEML VRHILDSIVV 70 80 90 100 110 120 APYLQGERFI DVGTGPGLPG IPLSIVRPEA HFTLLDSLGK RVRFLRQVQH ELKLENIEPV 130 140 150 160 170 180 QSRVEEFPSE PPFDGVISRA FASLNDMVSW CHHLPGEQGR FYALKGQMPE DEIALLPEEY 190 200 QVESVVKLQV PALDGERHLV VIKANKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS." Walker J.E., Gay N.J., Saraste M., Eberle A.N. Biochem. J. 224:799-815(1984) [PubMed: 6395859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The promoters of the atp operon of Escherichia coli K12." Nielsen J., Joergensen B.B., von Meyenburg K., Hansen F.G. Mol. Gen. Genet. 193:64-71(1984) [PubMed: 6318052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 192-207. Strain: K12.
[6]	"Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria." Okamoto S., Tamaru A., Nakajima C., Nishimura K., Tanaka Y., Tokuyama S., Suzuki Y., Ochi K. Mol. Microbiol. 63:1096-1106(2007) [PubMed: 17238915] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: K12.
[7]	"Crystal structure of the Escherichia coli glucose-inhibited division protein B (GidB) reveals a methyltransferase fold." Romanowski M.J., Bonanno J.B., Burley S.K. Proteins 47:563-567(2002) [PubMed: 12001236] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01631 Genomic DNA. Translation: CAA25774.1.
L10328 Genomic DNA. Translation: AAA62092.1.
U00096 Genomic DNA. Translation: AAC76763.1.
AP009048 Genomic DNA. Translation: BAE77548.1.
X01383 Genomic DNA. Translation: CAA25639.1.

PIR

BVECQB. C30389.

RefSeq

NP_418196.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JSX	X-ray	2.40	A	1-207	[»]

ProteinModelPortal

P0A6U5.

SMR

P0A6U5. Positions 1-207.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47992N.

IntAct

P0A6U5. 9 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000419; EBESCP00000000419; EBESCG00000000346.
EBESCT00000017232; EBESCP00000016523; EBESCG00000016291.

GeneID

948250.

GenomeReviews

Gene locus JW3718 in contig AP009048_GR.
Gene locus b3740 in contig U00096_GR.

KEGG

ecj:JW3718.
eco:b3740.

PATRIC

32122979. VBIEscCol129921_3865.

Organism-specific databases

EchoBASE

EB0371.

EcoGene

EG10376. rsmG.

Phylogenomic databases

eggNOG

COG0357.

GeneTree

EBGT00050000011126.

HOGENOM

HBG686577.

OMA

DSHFTLL.

PhylomeDB

P0A6U5.

ProtClustDB

PRK00107.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10376-MONOMER.
MetaCyc:EG10376-MONOMER.

Gene expression databases

Genevestigator

P0A6U5.

Family and domain databases

HAMAP

MF_00074. 16SrRNA_methyltr_G.
[Tree]

InterPro

IPR003682. rRNA_ssu_MeTfrase_G.
[Graphical view]

KO

K03501.

Pfam

PF02527. GidB. 1 hit.
[Graphical view]

PIRSF

PIRSF003078. GidB. 1 hit.

TIGRFAMs

TIGR00138. GidB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RSMG_ECOLI

Accession

Primary (citable) accession number: P0A6U5
Secondary accession number(s): P17113, Q2M858

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program