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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.3 Publications

Cofactori

FAD1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei180 – 1801FADBy similarity
Binding sitei370 – 3701FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186FADBy similarity
Nucleotide bindingi273 – 28715NADSequence analysisAdd
BLAST

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • oxidoreductase activity Source: InterPro

GO - Biological processi

  • tRNA methylation Source: EcoCyc
  • tRNA wobble uridine modification Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:EG10375-MONOMER.
ECOL316407:JW3719-MONOMER.
MetaCyc:EG10375-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene namesi
Name:mnmG
Synonyms:gidA, trmF
Ordered Locus Names:b3741, JW3719
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10375. mnmG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131G → A: Decrease in FAD binding and partial loss of activity. Loss of activity; when associated with A-15. 1 Publication
Mutagenesisi15 – 151G → A: Decrease in FAD binding and partial loss of activity. Loss of activity; when associated with A-13. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGPRO_0000117099Add
BLAST

Proteomic databases

EPDiP0A6U3.
PaxDbiP0A6U3.
PRIDEiP0A6U3.

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mnmEP255224EBI-550977,EBI-550986

Protein-protein interaction databases

BioGridi4263258. 136 interactions.
DIPiDIP-35786N.
IntActiP0A6U3. 12 interactions.
STRINGi511145.b3741.

Structurei

Secondary structure

1
629
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Helixi16 – 2712Combined sources
Beta strandi32 – 376Combined sources
Helixi39 – 413Combined sources
Beta strandi46 – 538Combined sources
Helixi56 – 6510Combined sources
Helixi70 – 778Combined sources
Beta strandi78 – 858Combined sources
Beta strandi86 – 883Combined sources
Helixi90 – 923Combined sources
Beta strandi94 – 996Combined sources
Helixi101 – 11313Combined sources
Beta strandi118 – 1225Combined sources
Beta strandi125 – 14016Combined sources
Beta strandi143 – 15311Combined sources
Turni157 – 1593Combined sources
Beta strandi162 – 1643Combined sources
Helixi182 – 1887Combined sources
Turni189 – 1913Combined sources
Beta strandi194 – 1974Combined sources
Beta strandi204 – 2063Combined sources
Helixi207 – 2093Combined sources
Helixi212 – 2143Combined sources
Beta strandi215 – 2184Combined sources
Beta strandi227 – 2304Combined sources
Helixi233 – 2353Combined sources
Beta strandi242 – 2465Combined sources
Helixi249 – 2579Combined sources
Helixi258 – 2614Combined sources
Helixi280 – 2867Combined sources
Beta strandi289 – 2913Combined sources
Beta strandi294 – 3007Combined sources
Beta strandi306 – 3105Combined sources
Helixi318 – 3269Combined sources
Beta strandi336 – 3383Combined sources
Beta strandi341 – 3488Combined sources
Helixi350 – 3523Combined sources
Beta strandi357 – 3637Combined sources
Beta strandi365 – 3673Combined sources
Helixi370 – 3723Combined sources
Helixi377 – 39519Combined sources
Turni405 – 4073Combined sources
Helixi409 – 42012Combined sources
Helixi426 – 4283Combined sources
Helixi429 – 4335Combined sources
Helixi434 – 4385Combined sources
Helixi441 – 4433Combined sources
Helixi444 – 45512Combined sources
Helixi460 – 48122Combined sources
Helixi492 – 4965Combined sources
Beta strandi499 – 5013Combined sources
Helixi509 – 5135Combined sources
Helixi520 – 5234Combined sources
Turni527 – 5293Combined sources
Helixi536 – 54712Combined sources
Turni549 – 5524Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CESX-ray2.41A/B/C/D1-629[»]
3CP2X-ray2.90A1-629[»]
ProteinModelPortaliP0A6U3.
SMRiP0A6U3. Positions 1-621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6U3.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.Curated

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201059.
InParanoidiP0A6U3.
KOiK03495.
OMAiFRPGYAI.
PhylomeDBiP0A6U3.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYPDPFDVI IIGGGHAGTE AAMAAARMGQ QTLLLTHNID TLGQMSCNPA
60 70 80 90 100
IGGIGKGHLV KEVDALGGLM AKAIDQAGIQ FRILNASKGP AVRATRAQAD
110 120 130 140 150
RVLYRQAVRT ALENQPNLMI FQQAVEDLIV ENDRVVGAVT QMGLKFRAKA
160 170 180 190 200
VVLTVGTFLD GKIHIGLDNY SGGRAGDPPS IPLSRRLREL PLRVGRLKTG
210 220 230 240 250
TPPRIDARTI DFSVLAQQHG DNPMPVFSFM GNASQHPQQV PCYITHTNEK
260 270 280 290 300
THDVIRSNLD RSPMYAGVIE GVGPRYCPSI EDKVMRFADR NQHQIFLEPE
310 320 330 340 350
GLTSNEIYPN GISTSLPFDV QMQIVRSMQG MENAKIVRPG YAIEYDFFDP
360 370 380 390 400
RDLKPTLESK FIQGLFFAGQ INGTTGYEEA AAQGLLAGLN AARLSADKEG
410 420 430 440 450
WAPARSQAYL GVLVDDLCTL GTKEPYRMFT SRAEYRLMLR EDNADLRLTE
460 470 480 490 500
IGRELGLVDD ERWARFNEKL ENIERERQRL KSTWVTPSAE AAAEVNAHLT
510 520 530 540 550
APLSREASGE DLLRRPEMTY EKLTTLTPFA PALTDEQAAE QVEIQVKYEG
560 570 580 590 600
YIARQQDEIE KQLRNENTLL PATLDYRQVS GLSNEVIAKL NDHKPASIGQ
610 620
ASRISGVTPA AISILLVWLK KQGMLRRSA
Length:629
Mass (Da):69,521
Last modified:March 29, 2005 - v1
Checksum:i54FD79807E901724
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti515 – 5151Missing (PubMed:6395859).Curated
Sequence conflicti515 – 5151Missing (PubMed:7686882).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25773.1.
L10328 Genomic DNA. Translation: AAA62093.1.
U00096 Genomic DNA. Translation: AAC76764.1.
AP009048 Genomic DNA. Translation: BAE77547.1.
K00826 Genomic DNA. Translation: AAA24250.2.
J01657 Genomic DNA. No translation available.
PIRiF65177. BVECQA.
RefSeqiNP_418197.1. NC_000913.3.
WP_000499788.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76764; AAC76764; b3741.
BAE77547; BAE77547; BAE77547.
GeneIDi948248.
KEGGiecj:JW3719.
eco:b3741.
PATRICi32122981. VBIEscCol129921_3866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25773.1.
L10328 Genomic DNA. Translation: AAA62093.1.
U00096 Genomic DNA. Translation: AAC76764.1.
AP009048 Genomic DNA. Translation: BAE77547.1.
K00826 Genomic DNA. Translation: AAA24250.2.
J01657 Genomic DNA. No translation available.
PIRiF65177. BVECQA.
RefSeqiNP_418197.1. NC_000913.3.
WP_000499788.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CESX-ray2.41A/B/C/D1-629[»]
3CP2X-ray2.90A1-629[»]
ProteinModelPortaliP0A6U3.
SMRiP0A6U3. Positions 1-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263258. 136 interactions.
DIPiDIP-35786N.
IntActiP0A6U3. 12 interactions.
STRINGi511145.b3741.

Proteomic databases

EPDiP0A6U3.
PaxDbiP0A6U3.
PRIDEiP0A6U3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76764; AAC76764; b3741.
BAE77547; BAE77547; BAE77547.
GeneIDi948248.
KEGGiecj:JW3719.
eco:b3741.
PATRICi32122981. VBIEscCol129921_3866.

Organism-specific databases

EchoBASEiEB0370.
EcoGeneiEG10375. mnmG.

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201059.
InParanoidiP0A6U3.
KOiK03495.
OMAiFRPGYAI.
PhylomeDBiP0A6U3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10375-MONOMER.
ECOL316407:JW3719-MONOMER.
MetaCyc:EG10375-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6U3.
PROiP0A6U3.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNMG_ECOLI
AccessioniPrimary (citable) accession number: P0A6U3
Secondary accession number(s): P03816, P17112, Q2M859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.