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Protein

Glycine cleavage system H protein

Gene

gcvH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.UniRule annotation1 Publication

Cofactori

(R)-lipoateUniRule annotationNote: Binds 1 lipoyl cofactor covalently.UniRule annotation

GO - Molecular functioni

  • lipoic acid binding Source: EcoliWiki

GO - Biological processi

  • glycine decarboxylation via glycine cleavage system Source: EcoliWiki

Enzyme and pathway databases

BioCyciEcoCyc:GCVH-MONOMER
MetaCyc:GCVH-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H proteinUniRule annotation
Gene namesi
Name:gcvH1 PublicationUniRule annotation
Ordered Locus Names:b2904, JW2872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10371 gcvH

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • glycine cleavage complex Source: InterPro

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001662182 – 129Glycine cleavage system H proteinAdd BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65N6-lipoyllysineUniRule annotation1

Proteomic databases

EPDiP0A6T9
PaxDbiP0A6T9
PRIDEiP0A6T9

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi426101943 interactors.
IntActiP0A6T9 2 interactors.
STRINGi316385.ECDH10B_3078

Structurei

Secondary structure

1129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 11Combined sources3
Beta strandi14 – 19Combined sources6
Beta strandi25 – 29Combined sources5
Helixi31 – 37Combined sources7
Beta strandi39 – 44Combined sources6
Beta strandi57 – 65Combined sources9
Beta strandi67 – 71Combined sources5
Beta strandi73 – 81Combined sources9
Helixi83 – 86Combined sources4
Helixi91 – 94Combined sources4
Turni96 – 100Combined sources5
Beta strandi103 – 108Combined sources6
Helixi110 – 115Combined sources6
Beta strandi116 – 118Combined sources3
Helixi119 – 127Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A7AX-ray3.10B/D2-129[»]
3A7LX-ray1.30A2-129[»]
3A8IX-ray1.99E/F1-129[»]
3A8JX-ray1.98E/F1-129[»]
3A8KX-ray1.95E/F1-129[»]
3AB9X-ray1.65A1-129[»]
ProteinModelPortaliP0A6T9
SMRiP0A6T9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6T9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 106Lipoyl-bindingUniRule annotationAdd BLAST83

Sequence similaritiesi

Belongs to the GcvH family.UniRule annotation

Keywords - Domaini

Lipoyl

Phylogenomic databases

eggNOGiCOG0509 LUCA
HOGENOMiHOG000239392
InParanoidiP0A6T9
KOiK02437
OMAiIVYVEVE
PhylomeDBiP0A6T9

Family and domain databases

CDDicd06848 GCS_H, 1 hit
HAMAPiMF_00272 GcvH, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR000089 Biotin_lipoyl
IPR002930 GCV_H
IPR033753 GCV_H/Fam206
IPR017453 GCV_H_sub
IPR011053 Single_hybrid_motif
PANTHERiPTHR11715 PTHR11715, 1 hit
PfamiView protein in Pfam
PF01597 GCV_H, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
TIGRFAMsiTIGR00527 gcvH, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVPAELKY SKEHEWLRKE ADGTYTVGIT EHAQELLGDM VFVDLPEVGA
60 70 80 90 100
TVSAGDDCAV AESVKAASDI YAPVSGEIVA VNDALSDSPE LVNSEPYAGG
110 120
WIFKIKASDE SELESLLDAT AYEALLEDE
Length:129
Mass (Da):13,811
Last modified:January 23, 2007 - v2
Checksum:i79748C818BD1325F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57690 Genomic DNA Translation: AAA68887.1
X73958 Genomic DNA Translation: CAA52145.1
U28377 Genomic DNA Translation: AAA69072.1
U00096 Genomic DNA Translation: AAC75942.1
M97263 Unassigned DNA Translation: AAC36844.1
L20872 Genomic DNA Translation: AAA23866.1
AP009048 Genomic DNA Translation: BAE76969.1
PIRiA56623
RefSeqiNP_417380.1, NC_000913.3
WP_001295377.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75942; AAC75942; b2904
BAE76969; BAE76969; BAE76969
GeneIDi947393
KEGGiecj:JW2872
eco:b2904
PATRICifig|1411691.4.peg.3828

Similar proteinsi

Entry informationi

Entry nameiGCSH_ECOLI
AccessioniPrimary (citable) accession number: P0A6T9
Secondary accession number(s): P23884, Q2M9T7, Q8Z3W9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome