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Protein

GTP cyclohydrolase 1

Gene

folE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Enzyme regulationi

Allosteric enzyme. Activity is modulated by K+, divalent cations, UTP, and tetrahydrobiopterin. Tetrahydrobiopterin is an inhibitor of this enzyme.

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (folE)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111Zinc1
Metal bindingi114Zinc1
Metal bindingi182Zinc1

GO - Molecular functioni

  • GTP binding Source: EcoliWiki
  • GTP cyclohydrolase I activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GTP-CYCLOHYDRO-I-MONOMER.
ECOL316407:JW2140-MONOMER.
MetaCyc:GTP-CYCLOHYDRO-I-MONOMER.
BRENDAi3.5.4.16. 2026.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:folE
Ordered Locus Names:b2153, JW2140
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11375. folE.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • protein complex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001194042 – 222GTP cyclohydrolase 1Add BLAST221

Proteomic databases

EPDiP0A6T5.
PaxDbiP0A6T5.
PRIDEiP0A6T5.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers.2 Publications

Protein-protein interaction databases

BioGridi4263524. 298 interactors.
853284. 1 interactor.
DIPiDIP-9676N.
IntActiP0A6T5. 1 interactor.
MINTiMINT-6732927.
STRINGi511145.b2153.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Helixi33 – 50Combined sources18
Helixi58 – 61Combined sources4
Helixi63 – 73Combined sources11
Turni74 – 76Combined sources3
Helixi77 – 79Combined sources3
Helixi81 – 83Combined sources3
Beta strandi89 – 91Combined sources3
Beta strandi100 – 111Combined sources12
Turni112 – 115Combined sources4
Beta strandi116 – 126Combined sources11
Beta strandi129 – 133Combined sources5
Helixi135 – 146Combined sources12
Beta strandi147 – 150Combined sources4
Helixi152 – 167Combined sources16
Beta strandi172 – 180Combined sources9
Helixi181 – 184Combined sources4
Beta strandi194 – 200Combined sources7
Helixi203 – 206Combined sources4
Helixi208 – 217Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8RX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1A9CX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1FBXX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1GTPX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T2-222[»]
1N3RX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1N3SX-ray2.55A/B/C/D/E/F/G/H/I/J2-222[»]
1N3TX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
ProteinModelPortaliP0A6T5.
SMRiP0A6T5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6T5.

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiENOG4105EUJ. Bacteria.
COG0302. LUCA.
HOGENOMiHOG000221222.
InParanoidiP0A6T5.
KOiK01495.
OMAiISGHMTE.
PhylomeDBiP0A6T5.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLSKEAAL VHEALVARGL ETPLRPPVHE MDNETRKSLI AGHMTEIMQL
60 70 80 90 100
LNLDLADDSL METPHRIAKM YVDEIFSGLD YANFPKITLI ENKMKVDEMV
110 120 130 140 150
TVRDITLTST CEHHFVTIDG KATVAYIPKD SVIGLSKINR IVQFFAQRPQ
160 170 180 190 200
VQERLTQQIL IALQTLLGTN NVAVSIDAVH YCVKARGIRD ATSATTTTSL
210 220
GGLFKSSQNT RHEFLRAVRH HN
Length:222
Mass (Da):24,831
Last modified:January 23, 2007 - v2
Checksum:i23C6A1440F579FC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63910 Genomic DNA. Translation: CAA45365.1.
U00007 Genomic DNA. Translation: AAA60535.1.
U00096 Genomic DNA. Translation: AAC75214.1.
AP009048 Genomic DNA. Translation: BAE76630.1.
PIRiH64983.
S29895.
RefSeqiNP_416658.1. NC_000913.3.
WP_001139613.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75214; AAC75214; b2153.
BAE76630; BAE76630; BAE76630.
GeneIDi949040.
KEGGiecj:JW2140.
eco:b2153.
PATRICi32119653. VBIEscCol129921_2237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63910 Genomic DNA. Translation: CAA45365.1.
U00007 Genomic DNA. Translation: AAA60535.1.
U00096 Genomic DNA. Translation: AAC75214.1.
AP009048 Genomic DNA. Translation: BAE76630.1.
PIRiH64983.
S29895.
RefSeqiNP_416658.1. NC_000913.3.
WP_001139613.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8RX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1A9CX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1FBXX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1GTPX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T2-222[»]
1N3RX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1N3SX-ray2.55A/B/C/D/E/F/G/H/I/J2-222[»]
1N3TX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
ProteinModelPortaliP0A6T5.
SMRiP0A6T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263524. 298 interactors.
853284. 1 interactor.
DIPiDIP-9676N.
IntActiP0A6T5. 1 interactor.
MINTiMINT-6732927.
STRINGi511145.b2153.

Proteomic databases

EPDiP0A6T5.
PaxDbiP0A6T5.
PRIDEiP0A6T5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75214; AAC75214; b2153.
BAE76630; BAE76630; BAE76630.
GeneIDi949040.
KEGGiecj:JW2140.
eco:b2153.
PATRICi32119653. VBIEscCol129921_2237.

Organism-specific databases

EchoBASEiEB1349.
EcoGeneiEG11375. folE.

Phylogenomic databases

eggNOGiENOG4105EUJ. Bacteria.
COG0302. LUCA.
HOGENOMiHOG000221222.
InParanoidiP0A6T5.
KOiK01495.
OMAiISGHMTE.
PhylomeDBiP0A6T5.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
BioCyciEcoCyc:GTP-CYCLOHYDRO-I-MONOMER.
ECOL316407:JW2140-MONOMER.
MetaCyc:GTP-CYCLOHYDRO-I-MONOMER.
BRENDAi3.5.4.16. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A6T5.
PROiP0A6T5.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCH1_ECOLI
AccessioniPrimary (citable) accession number: P0A6T5
Secondary accession number(s): P27511, Q2MAS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.