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Protein

Galactokinase

Gene

galK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). To a lesser extent, is also able to phosphorylate 2-deoxy-D-galactose and D-galactosamine. Is not able to use D-galacturonic acid, D-talose, L-altrose, and L-glucose as substrates.UniRule annotation3 Publications

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation3 Publications

Kineticsi

kcat is 108 min(-1), 30 min(-1) and 11.7 min(-1) with D-galactose, 2-deoxy-D-galactose, and D-galactosamine as substrate, respectively.

  1. KM=2.1 mM for D-galactose2 Publications
  2. KM=3.6 mM for 2-deoxy-D-galactose2 Publications
  3. KM=2.9 mM for D-galactosamine2 Publications
  4. KM=2.5 mM for ATP2 Publications
  1. Vmax=1.65 µmol/min/mg enzyme with D-galactose as substrate2 Publications

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei28Transition state stabilizerUniRule annotation1
Metal bindingi130MagnesiumUniRule annotation1
Metal bindingi162MagnesiumUniRule annotation1
Active sitei174Proton acceptorUniRule annotation1
Binding sitei223SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 130ATPUniRule annotation7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • galactokinase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

GO - Biological processi

  • galactose catabolic process via UDP-galactose Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GALACTOKIN-MONOMER.
ECOL316407:JW0740-MONOMER.
MetaCyc:GALACTOKIN-MONOMER.
SABIO-RKP0A6T3.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
Alternative name(s):
Galactose kinaseUniRule annotation
Gene namesi
Name:galKUniRule annotation
Synonyms:galA
Ordered Locus Names:b0757, JW0740
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10363. galK.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi371Y → H: Displays relaxed substrate specificity since it gains kinase activity toward sugars as diverse as D-galacturonic acid, D-talose, L-altrose, and L-glucose. Also shows enhanced turnover of the small pool of sugars converted by the wild-type enzyme. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001846072 – 382GalactokinaseAdd BLAST381

Proteomic databases

EPDiP0A6T3.
PaxDbiP0A6T3.
PRIDEiP0A6T3.

Interactioni

Protein-protein interaction databases

BioGridi4261711. 201 interactors.
IntActiP0A6T3. 4 interactors.
STRINGi511145.b0757.

Structurei

3D structure databases

ProteinModelPortaliP0A6T3.
SMRiP0A6T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 37Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CRD. Bacteria.
COG0153. LUCA.
HOGENOMiHOG000241100.
InParanoidiP0A6T3.
KOiK00849.
OMAiVMPCAIN.
PhylomeDBiP0A6T3.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase. 1 hit.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY
60 70 80 90 100
QTVISCAPRD DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV
110 120 130 140 150
KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD
160 170 180 190 200
GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS
210 220 230 240 250
MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF
260 270 280 290 300
NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS
310 320 330 340 350
MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP
360 370 380
AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC
Length:382
Mass (Da):41,442
Last modified:January 23, 2007 - v2
Checksum:i7D886CE6FC48F0A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02306 Genomic DNA. Translation: CAA26172.1.
U00096 Genomic DNA. Translation: AAC73844.1.
AP009048 Genomic DNA. Translation: BAA35419.1.
U13636 Genomic DNA. Translation: AAB17019.1.
PIRiB23044. KIECGG.
RefSeqiNP_415278.1. NC_000913.3.
WP_000053415.1. NZ_CP011343.2.

Genome annotation databases

EnsemblBacteriaiAAC73844; AAC73844; b0757.
BAA35419; BAA35419; BAA35419.
GeneIDi945358.
KEGGiecj:JW0740.
eco:b0757.
PATRICi32116715. VBIEscCol129921_0783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02306 Genomic DNA. Translation: CAA26172.1.
U00096 Genomic DNA. Translation: AAC73844.1.
AP009048 Genomic DNA. Translation: BAA35419.1.
U13636 Genomic DNA. Translation: AAB17019.1.
PIRiB23044. KIECGG.
RefSeqiNP_415278.1. NC_000913.3.
WP_000053415.1. NZ_CP011343.2.

3D structure databases

ProteinModelPortaliP0A6T3.
SMRiP0A6T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261711. 201 interactors.
IntActiP0A6T3. 4 interactors.
STRINGi511145.b0757.

Proteomic databases

EPDiP0A6T3.
PaxDbiP0A6T3.
PRIDEiP0A6T3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73844; AAC73844; b0757.
BAA35419; BAA35419; BAA35419.
GeneIDi945358.
KEGGiecj:JW0740.
eco:b0757.
PATRICi32116715. VBIEscCol129921_0783.

Organism-specific databases

EchoBASEiEB0358.
EcoGeneiEG10363. galK.

Phylogenomic databases

eggNOGiENOG4105CRD. Bacteria.
COG0153. LUCA.
HOGENOMiHOG000241100.
InParanoidiP0A6T3.
KOiK00849.
OMAiVMPCAIN.
PhylomeDBiP0A6T3.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciEcoCyc:GALACTOKIN-MONOMER.
ECOL316407:JW0740-MONOMER.
MetaCyc:GALACTOKIN-MONOMER.
SABIO-RKP0A6T3.

Miscellaneous databases

PROiP0A6T3.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase. 1 hit.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAL1_ECOLI
AccessioniPrimary (citable) accession number: P0A6T3
Secondary accession number(s): P06976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.