P0A6T3 (GAL1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactokinase
EC=2.7.1.6

Alternative name(s):
Galactose kinase

Gene names

Name:	galK
Synonyms:	galA
Ordered Locus Names:	b0757, JW0740

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP MF_00246
Subcellular location	Cytoplasm Potential HAMAP MF_00246.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	galactose catabolic process via UDP-galactose Inferred from mutant phenotype. Source: EcoliWiki
Cellular component	cytoplasm Inferred from direct assay. Source: EcoliWiki
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW galactokinase activity Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 382	381	Galactokinase HAMAP MF_00246		PRO_0000184607
Regions
	Nucleotide binding	122 – 132	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A6T3 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7D886CE6FC48F0A1	FASTA	382	41,442
	10 20 30 40 50 60 MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD 70 80 90 100 110 120 DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN 130 140 150 160 170 180 VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL 190 200 210 220 230 240 GKKDHALLID CRSLGTKAVS MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP 250 260 270 280 290 300 ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS 310 320 330 340 350 360 MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP AVQQAVAEQY 370 380 EAKTGIKETF YVCKPSQGAG QC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon." Debouck C., Riccio A., Schumperli D., McKenney K., Jeffers J., Hughes C., Rosenberg M., Heusterspreute M., Brunel F., Davison J. Nucleic Acids Res. 13:1841-1853(1985) [PubMed: 3158881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae." Schlesinger D.H., Schell M.A., Wilson D.B. FEBS Lett. 83:45-47(1977) [PubMed: 200486] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[6]	"Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli." Bouffard G.G., Rudd K.E., Adhya S.L. J. Mol. Biol. 244:269-278(1994) [PubMed: 7966338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 371-382.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02306 Genomic DNA. Translation: CAA26172.1. U00096 Genomic DNA. Translation: AAC73844.1. AP009048 Genomic DNA. Translation: BAA35419.1. U13636 Genomic DNA. Translation: AAB17019.1.
PIR	KIECGG. B23044.
RefSeq	NP_415278.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A6T3.
SMR	P0A6T3. Positions 2-381.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001753; EBESCP00000001753; EBESCG00000001445. EBESCT00000001754; EBESCP00000001754; EBESCG00000001445. EBESCT00000001755; EBESCP00000001755; EBESCG00000001445. EBESCT00000016992; EBESCP00000016283; EBESCG00000016051.
GeneID	945358.
GenomeReviews	Gene locus JW0740 in contig AP009048_GR. Gene locus b0757 in contig U00096_GR.
KEGG	ecj:JW0740. eco:b0757.
PATRIC	32116715. VBIEscCol129921_0783.
Organism-specific databases
EchoBASE	EB0358.
EcoGene	EG10363. galK.
Phylogenomic databases
eggNOG	COG0153.
GeneTree	EBGT00050000009538.
HOGENOM	HBG725121.
OMA	YNDGFVM.
PhylomeDB	P0A6T3.
ProtClustDB	PRK05101.
Enzyme and pathway databases
BioCyc	EcoCyc:GALACTOKIN-MONOMER. MetaCyc:GALACTOKIN-MONOMER.
Gene expression databases
Genevestigator	P0A6T3.
Family and domain databases
HAMAP	MF_00246. Galactokinase. [Tree]
InterPro	IPR000705. Galactokinase. IPR022963. Galactokinase_bac. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal-bd. IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00849.
Pfam	PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000530. Galactokinase. 1 hit.
PRINTS	PR00473. GALCTOKINASE. PR00959. MEVGALKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00131. Gal_kin. 1 hit.
PROSITE	PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL1_ECOLI

Accession

Primary (citable) accession number: P0A6T3
Secondary accession number(s): P06976

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program