Reviewed, UniProtKB/Swiss-Prot P0A6T1 (G6PI_ECOLI)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate isomerase
      Short name=GPI
    EC=5.3.1.9
Alternative name(s):
    Phosphoglucose isomerase
      Short name=PGI
    Phosphohexose isomerase
      Short name=PHI

Gene names

Name:	pgi
Ordered Locus Names:	b4025, JW3985

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP MF_00473
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP MF_00473
Subunit structure	Homodimer. HAMAP MF_00473
Subcellular location	Cytoplasm. HAMAP MF_00473
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Glucose-6-phosphate isomerase HAMAP MF_00473		PRO_0000180641
Sites
	Active site	355	1	Proton donor By similarity
	Active site	386	1	By similarity
	Active site	514	1	By similarity
Amino acid modifications
	Modified residue	80	1	N6-acetyllysine Ref.6
	Modified residue	228	1	N6-acetyllysine Ref.6
	Modified residue	234	1	N6-acetyllysine Ref.6
Experimental info
	Sequence conflict	317	1	L → V Ref.1
	Sequence conflict	317	1	L → V Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A6T1-1 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 74AEDB670A068A01	FASTA	549	61,530
	10 20 30 40 50 60 MKNINPTQTA AWQALQKHFD EMKDVTIADL FAKDGDRFSK FSATFDDQML VDYSKNRITE 70 80 90 100 110 120 ETLAKLQDLA KECDLAGAIK SMFSGEKINR TENRAVLHVA LRNRSNTPIL VDGKDVMPEV 130 140 150 160 170 180 NAVLEKMKTF SEAIISGEWK GYTGKAITDV VNIGIGGSDL GPYMVTEALR PYKNHLNMHF 190 200 210 220 230 240 VSNVDGTHIA EVLKKVNPET TLFLVASKTF TTQETMTNAH SARDWFLKAA GDEKHVAKHF 250 260 270 280 290 300 AALSTNAKAV GEFGIDTANM FEFWDWVGGR YSLWSAIGLS IVLSIGFDNF VELLSGAHAM 310 320 330 340 350 360 DKHFSTTPAE KNLPVLLALI GIWYNNFFGA ETEAILPYDQ YMHRFAAYFQ QGNMESNGKY 370 380 390 400 410 420 VDRNGNVVDY QTGPIIWGEP GTNGQHAFYQ LIHQGTKMVP CDFIAPAITH NPLSDHHQKL 430 440 450 460 470 480 LSNFFAQTEA LAFGKSREVV EQEYRDQGKD PATLDYVVPF KVFEGNRPTN SILLREITPF 490 500 510 520 530 540 SLGALIALYE HKIFTQGVIL NIFTFDQWGV ELGKQLANRI LPELKDDKEI SSHDSSTNGL INRYKAWRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli." Froman B.E., Tait R.C., Gottlieb L.D. Mol. Gen. Genet. 217:126-131(1989) [PubMed: 2549364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / JM101 / ATCC 33876 / DSM 3948.
[2]	"Anomalous phylogeny involving the enzyme glucose-6-phosphate isomerase." Smith M.W., Doolittle R.F. J. Mol. Evol. 34:544-545(1992) [PubMed: 1593646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PHYLOGENETIC STUDY. Strain: XL1 Blue 2.
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80; LYS-228 AND LYS-234, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15196 Genomic DNA. Translation: CAA33268.1. U00006 Genomic DNA. Translation: AAC43119.1. U00096 Genomic DNA. Translation: AAC76995.1. AP009048 Genomic DNA. Translation: BAE78027.1.
PIR	NUEC. H65209.
RefSeq	AP_004526.1. NP_418449.1.
3D structure databases
HSSP	HSSP built from PDB template 1IAT based on UniProtKB P06744.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A6T1. 7 interactions.
STRING	P0A6T1.
PTM databases
PhosSite	P0A6T1.
Genome annotation databases
GeneID	948535.
GenomeReviews	Gene locus JW3985 in contig AP009048_GR. Gene locus b4025 in contig U00096_GR.
KEGG	ecj:JW3985. eco:b4025.
Organism-specific databases
EchoBASE	EB0696.
EcoGene	EG10702. pgi.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A6T1.
OMA	GPKIVSQ.
Enzyme and pathway databases
BioCyc	EcoCyc:PGLUCISOM. MetaCyc:PGLUCISOM.
Gene expression databases
Genevestigator	P0A6T1.
Family and domain databases
HAMAP	MF_00473. [Tree]
InterPro	IPR001672. G6P_Isomerase. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
PANTHER	PTHR11469. G6P_Isomerase. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PI_ECOLI

Accession

Primary (citable) accession number: P0A6T1
Secondary accession number(s): P11537, Q2M6S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)