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Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by sorbitol-6-phosphate (S6P) and activated by CsrA.2 Publications

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase isozyme 2 (pfkB), ATP-dependent 6-phosphofructokinase isozyme 1 (pfkA)
  4. Fructose-bisphosphate aldolase class 2 (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei355Proton donorBy similarity1
Active sitei386By similarity1
Active sitei514By similarity1

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: EcoCyc

GO - Biological processi

  • cellular response to oxidative stress Source: EcoCyc
  • gluconeogenesis Source: UniProtKB-HAMAP
  • glycolytic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciEcoCyc:PGLUCISOM.
ECOL316407:JW3985-MONOMER.
MetaCyc:PGLUCISOM.
SABIO-RKP0A6T1.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgi
Ordered Locus Names:b4025, JW3985
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10702. pgi.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

One of the main features of pgi mutant is the overproduction of NADPH produced in pentose phosphate (PP) pathway which causes some reducing power imbalance that ultimately affects the cell growth. Cells lacking this gene grow slowly on glucose and utilize glucose primarily via the pentose phosphate pathway as the source of NADPH. They are also hypersensitive to oxidative stress induced by paraquat.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001806411 – 549Glucose-6-phosphate isomeraseAdd BLAST549

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei80N6-acetyllysine1 Publication1
Modified residuei228N6-acetyllysine1 Publication1
Modified residuei234N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A6T1.
PaxDbiP0A6T1.
PRIDEiP0A6T1.

Expressioni

Inductioni

Induced by oxidative stress.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-909327,EBI-909327

Protein-protein interaction databases

BioGridi4261959. 16 interactors.
DIPiDIP-35887N.
IntActiP0A6T1. 7 interactors.
STRINGi511145.b4025.

Structurei

Secondary structure

1549
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi10 – 22Combined sources13
Helixi27 – 33Combined sources7
Helixi37 – 40Combined sources4
Beta strandi41 – 45Combined sources5
Turni46 – 48Combined sources3
Beta strandi49 – 52Combined sources4
Beta strandi55 – 57Combined sources3
Helixi60 – 72Combined sources13
Helixi75 – 83Combined sources9
Turni90 – 93Combined sources4
Helixi98 – 101Combined sources4
Beta strandi114 – 116Combined sources3
Helixi117 – 135Combined sources19
Beta strandi149 – 153Combined sources5
Helixi156 – 158Combined sources3
Helixi160 – 168Combined sources9
Helixi170 – 172Combined sources3
Beta strandi177 – 181Combined sources5
Helixi186 – 193Combined sources8
Helixi198 – 200Combined sources3
Beta strandi201 – 206Combined sources6
Beta strandi208 – 210Combined sources3
Helixi213 – 230Combined sources18
Helixi233 – 238Combined sources6
Beta strandi240 – 244Combined sources5
Helixi247 – 250Combined sources4
Helixi257 – 259Combined sources3
Beta strandi260 – 262Combined sources3
Helixi269 – 271Combined sources3
Helixi276 – 278Combined sources3
Helixi279 – 285Combined sources7
Helixi287 – 306Combined sources20
Helixi309 – 311Combined sources3
Helixi313 – 326Combined sources14
Beta strandi332 – 338Combined sources7
Helixi340 – 342Combined sources3
Helixi345 – 357Combined sources13
Beta strandi375 – 377Combined sources3
Turni381 – 384Combined sources4
Helixi385 – 394Combined sources10
Beta strandi395 – 397Combined sources3
Beta strandi401 – 408Combined sources8
Helixi416 – 433Combined sources18
Helixi437 – 446Combined sources10
Helixi451 – 453Combined sources3
Turni455 – 457Combined sources3
Helixi458 – 461Combined sources4
Beta strandi469 – 476Combined sources8
Helixi479 – 499Combined sources21
Helixi508 – 510Combined sources3
Helixi511 – 523Combined sources13
Helixi535 – 547Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NBUX-ray2.05A/B/C/D/E/F1-549[»]
ProteinModelPortaliP0A6T1.
SMRiP0A6T1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6T1.

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
HOGENOMiHOG000261371.
InParanoidiP0A6T1.
KOiK01810.
OMAiSHLIAPY.
PhylomeDBiP0A6T1.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNINPTQTA AWQALQKHFD EMKDVTIADL FAKDGDRFSK FSATFDDQML
60 70 80 90 100
VDYSKNRITE ETLAKLQDLA KECDLAGAIK SMFSGEKINR TENRAVLHVA
110 120 130 140 150
LRNRSNTPIL VDGKDVMPEV NAVLEKMKTF SEAIISGEWK GYTGKAITDV
160 170 180 190 200
VNIGIGGSDL GPYMVTEALR PYKNHLNMHF VSNVDGTHIA EVLKKVNPET
210 220 230 240 250
TLFLVASKTF TTQETMTNAH SARDWFLKAA GDEKHVAKHF AALSTNAKAV
260 270 280 290 300
GEFGIDTANM FEFWDWVGGR YSLWSAIGLS IVLSIGFDNF VELLSGAHAM
310 320 330 340 350
DKHFSTTPAE KNLPVLLALI GIWYNNFFGA ETEAILPYDQ YMHRFAAYFQ
360 370 380 390 400
QGNMESNGKY VDRNGNVVDY QTGPIIWGEP GTNGQHAFYQ LIHQGTKMVP
410 420 430 440 450
CDFIAPAITH NPLSDHHQKL LSNFFAQTEA LAFGKSREVV EQEYRDQGKD
460 470 480 490 500
PATLDYVVPF KVFEGNRPTN SILLREITPF SLGALIALYE HKIFTQGVIL
510 520 530 540
NIFTFDQWGV ELGKQLANRI LPELKDDKEI SSHDSSTNGL INRYKAWRG
Length:549
Mass (Da):61,530
Last modified:March 29, 2005 - v1
Checksum:i74AEDB670A068A01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317L → V (PubMed:2549364).Curated1
Sequence conflicti317L → V (PubMed:1593646).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15196 Genomic DNA. Translation: CAA33268.1.
U00006 Genomic DNA. Translation: AAC43119.1.
U00096 Genomic DNA. Translation: AAC76995.1.
AP009048 Genomic DNA. Translation: BAE78027.1.
PIRiH65209. NUEC.
RefSeqiNP_418449.1. NC_000913.3.
WP_000789986.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76995; AAC76995; b4025.
BAE78027; BAE78027; BAE78027.
GeneIDi948535.
KEGGiecj:JW3985.
eco:b4025.
PATRICi32123579. VBIEscCol129921_4138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15196 Genomic DNA. Translation: CAA33268.1.
U00006 Genomic DNA. Translation: AAC43119.1.
U00096 Genomic DNA. Translation: AAC76995.1.
AP009048 Genomic DNA. Translation: BAE78027.1.
PIRiH65209. NUEC.
RefSeqiNP_418449.1. NC_000913.3.
WP_000789986.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NBUX-ray2.05A/B/C/D/E/F1-549[»]
ProteinModelPortaliP0A6T1.
SMRiP0A6T1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261959. 16 interactors.
DIPiDIP-35887N.
IntActiP0A6T1. 7 interactors.
STRINGi511145.b4025.

Proteomic databases

EPDiP0A6T1.
PaxDbiP0A6T1.
PRIDEiP0A6T1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76995; AAC76995; b4025.
BAE78027; BAE78027; BAE78027.
GeneIDi948535.
KEGGiecj:JW3985.
eco:b4025.
PATRICi32123579. VBIEscCol129921_4138.

Organism-specific databases

EchoBASEiEB0696.
EcoGeneiEG10702. pgi.

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
HOGENOMiHOG000261371.
InParanoidiP0A6T1.
KOiK01810.
OMAiSHLIAPY.
PhylomeDBiP0A6T1.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BioCyciEcoCyc:PGLUCISOM.
ECOL316407:JW3985-MONOMER.
MetaCyc:PGLUCISOM.
SABIO-RKP0A6T1.

Miscellaneous databases

EvolutionaryTraceiP0A6T1.
PROiP0A6T1.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_ECOLI
AccessioniPrimary (citable) accession number: P0A6T1
Secondary accession number(s): P11537, Q2M6S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.