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Protein

Glycerol-3-phosphate dehydrogenase [NAD(P)+]

Gene

gpsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NAD; via amide nitrogenUniRule annotation1
Binding sitei110SubstrateUniRule annotation1
Binding sitei143NAD; via amide nitrogenUniRule annotation1
Active sitei195Proton acceptorUniRule annotation1
Binding sitei259NADUniRule annotation1
Binding sitei285NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerophospholipid biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.
MetaCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+]UniRule annotation (EC:1.1.1.94UniRule annotation)
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gpsAUniRule annotation
Ordered Locus Names:b3608, JW3583
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20091. gpsA.

Subcellular locationi

P0A6S7:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • glycerol-3-phosphate dehydrogenase complex Source: InterPro

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001379571 – 339Glycerol-3-phosphate dehydrogenase [NAD(P)+]Add BLAST339

Proteomic databases

PaxDbiP0A6S7.
PRIDEiP0A6S7.

Interactioni

Protein-protein interaction databases

BioGridi4261595. 125 interactors.
DIPiDIP-48003N.
IntActiP0A6S7. 6 interactors.
STRINGi316385.ECDH10B_3790.

Structurei

3D structure databases

ProteinModelPortaliP0A6S7.
SMRiP0A6S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 260Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSF. Bacteria.
COG0240. LUCA.
HOGENOMiHOG000246854.
InParanoidiP0A6S7.
KOiK00057.
PhylomeDBiP0A6S7.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00957. NAD_G3PDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQRNASMTV IGAGSYGTAL AITLARNGHE VVLWGHDPEH IATLERDRCN
60 70 80 90 100
AAFLPDVPFP DTLHLESDLA TALAASRNIL VVVPSHVFGE VLRQIKPLMR
110 120 130 140 150
PDARLVWATK GLEAETGRLL QDVAREALGD QIPLAVISGP TFAKELAAGL
160 170 180 190 200
PTAISLASTD QTFADDLQQL LHCGKSFRVY SNPDFIGVQL GGAVKNVIAI
210 220 230 240 250
GAGMSDGIGF GANARTALIT RGLAEMSRLG AALGADPATF MGMAGLGDLV
260 270 280 290 300
LTCTDNQSRN RRFGMMLGQG MDVQSAQEKI GQVVEGYRNT KEVRELAHRF
310 320 330
GVEMPITEEI YQVLYCGKNA REAALTLLGR ARKDERSSH
Length:339
Mass (Da):36,362
Last modified:March 29, 2005 - v1
Checksum:iDF95F674204276BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18585.1.
U00096 Genomic DNA. Translation: AAC76632.1.
AP009048 Genomic DNA. Translation: BAE77684.1.
PIRiS47829.
RefSeqiNP_418065.1. NC_000913.3.
WP_001076194.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76632; AAC76632; b3608.
BAE77684; BAE77684; BAE77684.
GeneIDi948125.
KEGGiecj:JW3583.
eco:b3608.
PATRICifig|1411691.4.peg.3098.

Similar proteinsi

Entry informationi

Entry nameiGPDA_ECOLI
AccessioniPrimary (citable) accession number: P0A6S7
Secondary accession number(s): P37606, Q2M7S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: October 25, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families