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Protein

Glycerol-3-phosphate dehydrogenase [NAD(P)+]

Gene

gpsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101NAD; via amide nitrogenUniRule annotation
Binding sitei110 – 1101SubstrateUniRule annotation
Binding sitei143 – 1431NAD; via amide nitrogenUniRule annotation
Active sitei195 – 1951Proton acceptorUniRule annotation
Binding sitei259 – 2591NADUniRule annotation
Binding sitei285 – 2851NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.
ECOL316407:JW3583-MONOMER.
MetaCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+]UniRule annotation (EC:1.1.1.94UniRule annotation)
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gpsAUniRule annotation
Ordered Locus Names:b3608, JW3583
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20091. gpsA.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Glycerol-3-phosphate dehydrogenase [NAD(P)+]PRO_0000137957Add
BLAST

Proteomic databases

EPDiP0A6S7.
PaxDbiP0A6S7.
PRIDEiP0A6S7.

Interactioni

Protein-protein interaction databases

BioGridi4261595. 125 interactions.
DIPiDIP-48003N.
IntActiP0A6S7. 6 interactions.
STRINGi511145.b3608.

Structurei

3D structure databases

ProteinModelPortaliP0A6S7.
SMRiP0A6S7. Positions 8-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni259 – 2602Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSF. Bacteria.
COG0240. LUCA.
HOGENOMiHOG000246854.
InParanoidiP0A6S7.
KOiK00057.
OMAiSAQMEMS.
PhylomeDBiP0A6S7.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQRNASMTV IGAGSYGTAL AITLARNGHE VVLWGHDPEH IATLERDRCN
60 70 80 90 100
AAFLPDVPFP DTLHLESDLA TALAASRNIL VVVPSHVFGE VLRQIKPLMR
110 120 130 140 150
PDARLVWATK GLEAETGRLL QDVAREALGD QIPLAVISGP TFAKELAAGL
160 170 180 190 200
PTAISLASTD QTFADDLQQL LHCGKSFRVY SNPDFIGVQL GGAVKNVIAI
210 220 230 240 250
GAGMSDGIGF GANARTALIT RGLAEMSRLG AALGADPATF MGMAGLGDLV
260 270 280 290 300
LTCTDNQSRN RRFGMMLGQG MDVQSAQEKI GQVVEGYRNT KEVRELAHRF
310 320 330
GVEMPITEEI YQVLYCGKNA REAALTLLGR ARKDERSSH
Length:339
Mass (Da):36,362
Last modified:March 29, 2005 - v1
Checksum:iDF95F674204276BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18585.1.
U00096 Genomic DNA. Translation: AAC76632.1.
AP009048 Genomic DNA. Translation: BAE77684.1.
PIRiS47829.
RefSeqiNP_418065.1. NC_000913.3.
WP_001076194.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76632; AAC76632; b3608.
BAE77684; BAE77684; BAE77684.
GeneIDi948125.
KEGGiecj:JW3583.
eco:b3608.
PATRICi32122701. VBIEscCol129921_3727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18585.1.
U00096 Genomic DNA. Translation: AAC76632.1.
AP009048 Genomic DNA. Translation: BAE77684.1.
PIRiS47829.
RefSeqiNP_418065.1. NC_000913.3.
WP_001076194.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A6S7.
SMRiP0A6S7. Positions 8-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261595. 125 interactions.
DIPiDIP-48003N.
IntActiP0A6S7. 6 interactions.
STRINGi511145.b3608.

Proteomic databases

EPDiP0A6S7.
PaxDbiP0A6S7.
PRIDEiP0A6S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76632; AAC76632; b3608.
BAE77684; BAE77684; BAE77684.
GeneIDi948125.
KEGGiecj:JW3583.
eco:b3608.
PATRICi32122701. VBIEscCol129921_3727.

Organism-specific databases

EchoBASEiEB4142.
EcoGeneiEG20091. gpsA.

Phylogenomic databases

eggNOGiENOG4105CSF. Bacteria.
COG0240. LUCA.
HOGENOMiHOG000246854.
InParanoidiP0A6S7.
KOiK00057.
OMAiSAQMEMS.
PhylomeDBiP0A6S7.

Enzyme and pathway databases

UniPathwayiUPA00940.
BioCyciEcoCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.
ECOL316407:JW3583-MONOMER.
MetaCyc:GLYC3PDEHYDROGBIOSYN-MONOMER.

Miscellaneous databases

PROiP0A6S7.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDA_ECOLI
AccessioniPrimary (citable) accession number: P0A6S7
Secondary accession number(s): P37606, Q2M7S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.