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P0A6R3

- FIS_ECOLI

UniProt

P0A6R3 - FIS_ECOLI

Protein

DNA-binding protein Fis

Gene

fis

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi74 – 9320H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: EcoCyc
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: EcoliWiki
    4. sequence-specific DNA binding transcription factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: EcoCyc
    2. regulation of transcription by chromatin organization Source: EcoCyc
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PD00196.
    ECOL316407:JW3229-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein FisUniRule annotation
    Alternative name(s):
    Factor-for-inversion stimulation protein
    Hin recombinational enhancer-binding protein
    Gene namesi
    Name:fisUniRule annotation
    Ordered Locus Names:b3261, JW3229
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10317. fis.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic nucleosome Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9898DNA-binding protein FisPRO_0000203878Add
    BLAST

    Proteomic databases

    PaxDbiP0A6R3.
    PRIDEiP0A6R3.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6R3.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    yebVP645033EBI-550170,EBI-9126792

    Protein-protein interaction databases

    DIPiDIP-47975N.
    IntActiP0A6R3. 25 interactions.
    MINTiMINT-1225073.
    STRINGi511145.b3261.

    Structurei

    Secondary structure

    1
    98
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 154
    Turni18 – 203
    Beta strandi23 – 264
    Helixi27 – 4216
    Helixi50 – 6920
    Turni70 – 723
    Helixi74 – 818
    Helixi85 – 9410

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ETKX-ray2.10A/B1-98[»]
    1ETOX-ray1.90A/B1-98[»]
    1ETQX-ray2.80A/B/C/D1-98[»]
    1ETVX-ray2.00A/B1-98[»]
    1ETWX-ray2.00A/B1-98[»]
    1ETXX-ray1.90A/B1-98[»]
    1ETYX-ray2.00A/B1-98[»]
    1F36X-ray2.65A/B1-98[»]
    1FIAX-ray2.00A/B1-98[»]
    1FIPX-ray1.90A/B1-98[»]
    3FISX-ray2.30A/B1-98[»]
    3IV5X-ray2.90A/B1-98[»]
    3JR9X-ray2.90A/B1-98[»]
    3JRAX-ray3.11A/B1-98[»]
    3JRBX-ray3.10A/B1-98[»]
    3JRCX-ray3.08A/B1-98[»]
    3JRDX-ray3.10A/B1-98[»]
    3JREX-ray3.17A/B1-98[»]
    3JRFX-ray3.05A/B1-98[»]
    3JRGX-ray3.11A/B1-98[»]
    3JRHX-ray2.88A/B1-98[»]
    3JRIX-ray3.11A/B1-98[»]
    4FISX-ray2.30A/B1-98[»]
    DisProtiDP00422.
    ProteinModelPortaliP0A6R3.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A6R3.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni17 – 4428Required for the stimulation of HIN-mediated recombinationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transcriptional regulatory Fis family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2901.
    HOGENOMiHOG000256331.
    KOiK03557.
    OMAiHNQTRAA.
    OrthoDBiEOG67HJXZ.
    PhylomeDBiP0A6R3.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    HAMAPiMF_00166. DNA_binding_Fis.
    InterProiIPR005412. Fis_DNA-bd.
    IPR009057. Homeodomain-like.
    IPR002197. HTH_Fis.
    [Graphical view]
    PfamiPF02954. HTH_8. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002097. DNA-binding_Fis. 1 hit.
    PRINTSiPR01591. DNABINDNGFIS.
    PR01590. HTHFIS.
    SUPFAMiSSF46689. SSF46689. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6R3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFEQRVNSDV LTVSTVNSQD QVTQKPLRDS VKQALKNYFA QLNGQDVNDL   50
    YELVLAEVEQ PLLDMVMQYT RGNQTRAALM MGINRGTLRK KLKKYGMN 98
    Length:98
    Mass (Da):11,240
    Last modified:May 10, 2005 - v1
    Checksum:i2E339BFCFCDB163C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03245 Genomic DNA. Translation: AAA83856.1.
    J03816 Genomic DNA. Translation: AAA98812.1.
    M95784 Genomic DNA. Translation: AAA23783.1.
    U18997 Genomic DNA. Translation: AAA58065.1.
    U00096 Genomic DNA. Translation: AAC76293.1.
    AP009048 Genomic DNA. Translation: BAE77302.1.
    PIRiA32142. DNECFS.
    RefSeqiNP_417727.1. NC_000913.3.
    YP_491443.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76293; AAC76293; b3261.
    BAE77302; BAE77302; BAE77302.
    GeneIDi12932870.
    947697.
    KEGGiecj:Y75_p3179.
    eco:b3261.
    PATRICi32121952. VBIEscCol129921_3360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03245 Genomic DNA. Translation: AAA83856.1 .
    J03816 Genomic DNA. Translation: AAA98812.1 .
    M95784 Genomic DNA. Translation: AAA23783.1 .
    U18997 Genomic DNA. Translation: AAA58065.1 .
    U00096 Genomic DNA. Translation: AAC76293.1 .
    AP009048 Genomic DNA. Translation: BAE77302.1 .
    PIRi A32142. DNECFS.
    RefSeqi NP_417727.1. NC_000913.3.
    YP_491443.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ETK X-ray 2.10 A/B 1-98 [» ]
    1ETO X-ray 1.90 A/B 1-98 [» ]
    1ETQ X-ray 2.80 A/B/C/D 1-98 [» ]
    1ETV X-ray 2.00 A/B 1-98 [» ]
    1ETW X-ray 2.00 A/B 1-98 [» ]
    1ETX X-ray 1.90 A/B 1-98 [» ]
    1ETY X-ray 2.00 A/B 1-98 [» ]
    1F36 X-ray 2.65 A/B 1-98 [» ]
    1FIA X-ray 2.00 A/B 1-98 [» ]
    1FIP X-ray 1.90 A/B 1-98 [» ]
    3FIS X-ray 2.30 A/B 1-98 [» ]
    3IV5 X-ray 2.90 A/B 1-98 [» ]
    3JR9 X-ray 2.90 A/B 1-98 [» ]
    3JRA X-ray 3.11 A/B 1-98 [» ]
    3JRB X-ray 3.10 A/B 1-98 [» ]
    3JRC X-ray 3.08 A/B 1-98 [» ]
    3JRD X-ray 3.10 A/B 1-98 [» ]
    3JRE X-ray 3.17 A/B 1-98 [» ]
    3JRF X-ray 3.05 A/B 1-98 [» ]
    3JRG X-ray 3.11 A/B 1-98 [» ]
    3JRH X-ray 2.88 A/B 1-98 [» ]
    3JRI X-ray 3.11 A/B 1-98 [» ]
    4FIS X-ray 2.30 A/B 1-98 [» ]
    DisProti DP00422.
    ProteinModelPortali P0A6R3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47975N.
    IntActi P0A6R3. 25 interactions.
    MINTi MINT-1225073.
    STRINGi 511145.b3261.

    Proteomic databases

    PaxDbi P0A6R3.
    PRIDEi P0A6R3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76293 ; AAC76293 ; b3261 .
    BAE77302 ; BAE77302 ; BAE77302 .
    GeneIDi 12932870.
    947697.
    KEGGi ecj:Y75_p3179.
    eco:b3261.
    PATRICi 32121952. VBIEscCol129921_3360.

    Organism-specific databases

    EchoBASEi EB0313.
    EcoGenei EG10317. fis.

    Phylogenomic databases

    eggNOGi COG2901.
    HOGENOMi HOG000256331.
    KOi K03557.
    OMAi HNQTRAA.
    OrthoDBi EOG67HJXZ.
    PhylomeDBi P0A6R3.

    Enzyme and pathway databases

    BioCyci EcoCyc:PD00196.
    ECOL316407:JW3229-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A6R3.
    PROi P0A6R3.

    Gene expression databases

    Genevestigatori P0A6R3.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    HAMAPi MF_00166. DNA_binding_Fis.
    InterProi IPR005412. Fis_DNA-bd.
    IPR009057. Homeodomain-like.
    IPR002197. HTH_Fis.
    [Graphical view ]
    Pfami PF02954. HTH_8. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002097. DNA-binding_Fis. 1 hit.
    PRINTSi PR01591. DNABINDNGFIS.
    PR01590. HTHFIS.
    SUPFAMi SSF46689. SSF46689. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of the gene encoding the Hin recombinational enhancer binding protein."
      Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE.
    2. "Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene."
      Koch C., Vanderkerckhove J., Kahmann R.
      Proc. Natl. Acad. Sci. U.S.A. 85:4237-4241(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-29.
    3. "Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
      Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
      J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis."
      Green J., Anjum M.F., Guest J.R.
      Mol. Microbiol. 20:1043-1055(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-11.
    7. "E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo."
      Ross W., Thompson J.F., Newlands J.T., Gourse R.L.
      EMBO J. 9:3733-3742(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro."
      Wold S., Crooke E., Skarstad K.
      Nucleic Acids Res. 24:3527-3532(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision."
      Osuna R., Finkel S.E., Johnson R.C.
      EMBO J. 10:1593-1603(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, DOMAINS.
    10. "The regulation of the Escherichia coli mazEF promoter involves an unusual alternating palindrome."
      Marianovsky I., Aizenman E., Engelberg-Kulka H., Glaser G.
      J. Biol. Chem. 276:5975-5984(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: BINDING TO THE MAZE-MAZF PROMOTER.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    11. "Three-dimensional structure of the E. coli DNA-binding protein FIS."
      Kostrewa D., Granzin J., Koch C., Choe H.-W., Raghunathan S., Wolf W., Labahn J., Kahmann R., Saenger W.
      Nature 349:178-180(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    12. "Crystal structure of the factor for inversion stimulation FIS at 2.0-A resolution."
      Kostrewa D., Granzin J., Stock D., Choe H.-W., Labahn J., Saenger W.
      J. Mol. Biol. 226:209-226(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    13. "The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms."
      Safo M.K., Yang W.Z., Corselli L., Cramton S.E., Yuan H.S., Johnson R.C.
      EMBO J. 16:6860-6873(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).
    14. "Structural analysis of the transcriptional activation region on fis: crystal structures of six fis mutants with different activation properties."
      Cheng Y.-S., Yang W.-Z., Johnson R.C., Yuan H.S.
      J. Mol. Biol. 302:1139-1151(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANTS.

    Entry informationi

    Entry nameiFIS_ECOLI
    AccessioniPrimary (citable) accession number: P0A6R3
    Secondary accession number(s): P11028, P37404, Q2M8V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3