DNA-binding protein fis - Escherichia coli (strain K12)

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P0A6R3 (FIS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA-binding protein fis

Alternative name(s):
Factor-for-inversion stimulation protein
Hin recombinational enhancer-binding protein

Gene names

Name:	fis
Ordered Locus Names:	b3261, JW3229

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	98 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Ref.7 Ref.8
Subunit structure	Homodimer.
Sequence similarities	Belongs to the transcriptional regulatory fis family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription by chromatin organization Inferred from direct assay PubMed 11812843. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasmic nucleosome Inferred from direct assay PubMed 10320479. Source: EcoCyc
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 10551881. Source: EcoliWiki sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yhbT	P64599	1	EBI-550170,EBI-1127089

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 98

DNA-binding protein fis HAMAP-Rule MF_00166

PRO_0000203878

Regions

DNA binding

74 – 93

H-T-H motif HAMAP-Rule MF_00166

Region

17 – 44

Required for the stimulation of HIN-mediated recombination HAMAP-Rule MF_00166

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A6R3 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 2E339BFCFCDB163C
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FASTA

11,240

        10         20         30         40         50         60 
MFEQRVNSDV LTVSTVNSQD QVTQKPLRDS VKQALKNYFA QLNGQDVNDL YELVLAEVEQ 

        70         80         90 
PLLDMVMQYT RGNQTRAALM MGINRGTLRK KLKKYGMN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the gene encoding the Hin recombinational enhancer binding protein." Johnson R.C., Ball C.A., Pfeffer D., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE.
[2]	"Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene." Koch C., Vanderkerckhove J., Kahmann R. Proc. Natl. Acad. Sci. U.S.A. 85:4237-4241(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-29.
[3]	"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli." Ball C.A., Osuna R., Ferguson K.C., Johnson R.C. J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis." Green J., Anjum M.F., Guest J.R. Mol. Microbiol. 20:1043-1055(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11.
[7]	"E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo." Ross W., Thompson J.F., Newlands J.T., Gourse R.L. EMBO J. 9:3733-3742(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro." Wold S., Crooke E., Skarstad K. Nucleic Acids Res. 24:3527-3532(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision." Osuna R., Finkel S.E., Johnson R.C. EMBO J. 10:1593-1603(1991) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS, DOMAINS.
[10]	"The regulation of the Escherichia coli mazEF promoter involves an unusual alternating palindrome." Marianovsky I., Aizenman E., Engelberg-Kulka H., Glaser G. J. Biol. Chem. 276:5975-5984(2001) [PubMed] [Europe PMC] [Abstract] Cited for: BINDING TO THE MAZE-MAZF PROMOTER. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[11]	"Three-dimensional structure of the E. coli DNA-binding protein FIS." Kostrewa D., Granzin J., Koch C., Choe H.-W., Raghunathan S., Wolf W., Labahn J., Kahmann R., Saenger W. Nature 349:178-180(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[12]	"Crystal structure of the factor for inversion stimulation FIS at 2.0-A resolution." Kostrewa D., Granzin J., Stock D., Choe H.-W., Labahn J., Saenger W. J. Mol. Biol. 226:209-226(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[13]	"The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms." Safo M.K., Yang W.Z., Corselli L., Cramton S.E., Yuan H.S., Johnson R.C. EMBO J. 16:6860-6873(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).
[14]	"Structural analysis of the transcriptional activation region on fis: crystal structures of six fis mutants with different activation properties." Cheng Y.-S., Yang W.-Z., Johnson R.C., Yuan H.S. J. Mol. Biol. 302:1139-1151(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANTS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03245 Genomic DNA. Translation: AAA83856.1.
J03816 Genomic DNA. Translation: AAA98812.1.
M95784 Genomic DNA. Translation: AAA23783.1.
U18997 Genomic DNA. Translation: AAA58065.1.
U00096 Genomic DNA. Translation: AAC76293.1.
AP009048 Genomic DNA. Translation: BAE77302.1.

PIR

DNECFS. A32142.

RefSeq

NP_417727.1. NC_000913.2.
YP_491443.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ETK	X-ray	2.10	A/B	1-98	[»]
1ETO	X-ray	1.90	A/B	1-98	[»]
1ETQ	X-ray	2.80	A/B/C/D	1-98	[»]
1ETV	X-ray	2.00	A/B	1-98	[»]
1ETW	X-ray	2.00	A/B	1-98	[»]
1ETX	X-ray	1.90	A/B	1-98	[»]
1ETY	X-ray	2.00	A/B	1-98	[»]
1F36	X-ray	2.65	A/B	1-98	[»]
1FIA	X-ray	2.00	A/B	1-98	[»]
1FIP	X-ray	1.90	A/B	1-98	[»]
3FIS	X-ray	2.30	A/B	1-98	[»]
3IV5	X-ray	2.90	A/B	1-98	[»]
3JR9	X-ray	2.90	A/B	1-98	[»]
3JRA	X-ray	3.11	A/B	1-98	[»]
3JRB	X-ray	3.10	A/B	1-98	[»]
3JRC	X-ray	3.08	A/B	1-98	[»]
3JRD	X-ray	3.10	A/B	1-98	[»]
3JRE	X-ray	3.17	A/B	1-98	[»]
3JRF	X-ray	3.05	A/B	1-98	[»]
3JRG	X-ray	3.11	A/B	1-98	[»]
3JRH	X-ray	2.88	A/B	1-98	[»]
3JRI	X-ray	3.11	A/B	1-98	[»]
4FIS	X-ray	2.30	A/B	1-98	[»]

DisProt

DP00422.

ProteinModelPortal

P0A6R3.

SMR

P0A6R3. Positions 1-98.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47975N.

IntAct

P0A6R3. 24 interactions.

MINT

MINT-1225073.

STRING

511145.b3261.

Proteomic databases

PaxDb

P0A6R3.

PRIDE

P0A6R3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76293; AAC76293; b3261.
BAE77302; BAE77302; BAE77302.

GeneID

12932870.
947697.

KEGG

ecj:Y75_p3179.
eco:b3261.

PATRIC

32121952. VBIEscCol129921_3360.

Organism-specific databases

EchoBASE

EB0313.

EcoGene

EG10317. fis.

Phylogenomic databases

eggNOG

COG2901.

HOGENOM

HOG000256331.

K03557.

OMA

HNQTRAA.

ProtClustDB

PRK00430.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00196.
ECOL316407:JW3229-MONOMER.

Gene expression databases

Genevestigator

P0A6R3.

Family and domain databases

Gene3D

1.10.10.60. 1 hit.

HAMAP

MF_00166. DNA-binding_Fis.

InterPro

IPR005412. Fis_DNA-bd.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
[Graphical view]

Pfam

PF02954. HTH_8. 1 hit.
[Graphical view]

PIRSF

PIRSF002097. DNA-binding_Fis. 1 hit.

PRINTS

PR01591. DNABINDNGFIS.
PR01590. HTHFIS.

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A6R3.

Entry information

Entry name

FIS_ECOLI

Accession

Primary (citable) accession number: P0A6R3
Secondary accession number(s): P11028, P37404, Q2M8V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents