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Protein

DNA-binding protein Fis

Gene

fis

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Binds to hundreds of transcriptionally active and inactive AT-rich sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi74 – 9320H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoliWiki
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00196.
ECOL316407:JW3229-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein FisUniRule annotation
Alternative name(s):
Factor-for-inversion stimulation protein
Hin recombinational enhancer-binding protein
Gene namesi
Name:fisUniRule annotation
Ordered Locus Names:b3261, JW3229
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10317. fis.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic nucleosome Source: EcoCyc
  • cytosol Source: EcoCyc
  • nucleoid Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9898DNA-binding protein FisPRO_0000203878Add
BLAST

Proteomic databases

EPDiP0A6R3.
PaxDbiP0A6R3.
PRIDEiP0A6R3.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000770.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
yebVP645033EBI-550170,EBI-9126792

Protein-protein interaction databases

BioGridi4261864. 53 interactions.
DIPiDIP-47975N.
IntActiP0A6R3. 25 interactions.
MINTiMINT-1225073.
STRINGi511145.b3261.

Structurei

Secondary structure

1
98
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154Combined sources
Turni18 – 203Combined sources
Beta strandi23 – 264Combined sources
Helixi27 – 4216Combined sources
Helixi50 – 6920Combined sources
Turni70 – 723Combined sources
Helixi74 – 818Combined sources
Helixi85 – 9410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ETKX-ray2.10A/B1-98[»]
1ETOX-ray1.90A/B1-98[»]
1ETQX-ray2.80A/B/C/D1-98[»]
1ETVX-ray2.00A/B1-98[»]
1ETWX-ray2.00A/B1-98[»]
1ETXX-ray1.90A/B1-98[»]
1ETYX-ray2.00A/B1-98[»]
1F36X-ray2.65A/B1-98[»]
1FIAX-ray2.00A/B1-98[»]
1FIPX-ray1.90A/B1-98[»]
3FISX-ray2.30A/B1-98[»]
3IV5X-ray2.90A/B1-98[»]
3JR9X-ray2.90A/B1-98[»]
3JRAX-ray3.11A/B1-98[»]
3JRBX-ray3.10A/B1-98[»]
3JRCX-ray3.08A/B1-98[»]
3JRDX-ray3.10A/B1-98[»]
3JREX-ray3.17A/B1-98[»]
3JRFX-ray3.05A/B1-98[»]
3JRGX-ray3.11A/B1-98[»]
3JRHX-ray2.88A/B1-98[»]
3JRIX-ray3.11A/B1-98[»]
4FISX-ray2.30A/B1-98[»]
5DTDX-ray2.64A/B1-98[»]
5E3LX-ray2.66A/B1-98[»]
5E3MX-ray2.89A/B1-98[»]
5E3NX-ray2.66A/B1-98[»]
5E3OX-ray2.78A/B1-98[»]
DisProtiDP00422.
ProteinModelPortaliP0A6R3.
SMRiP0A6R3. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6R3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 4428Required for the stimulation of HIN-mediated recombinationAdd
BLAST

Sequence similaritiesi

Belongs to the transcriptional regulatory Fis family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2901. LUCA.
HOGENOMiHOG000256331.
InParanoidiP0A6R3.
KOiK03557.
OMAiMSDNANL.
PhylomeDBiP0A6R3.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
HAMAPiMF_00166. DNA_binding_Fis. 1 hit.
InterProiIPR005412. Fis_DNA-bd.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF002097. DNA-binding_Fis. 1 hit.
PRINTSiPR01591. DNABINDNGFIS.
PR01590. HTHFIS.
SUPFAMiSSF46689. SSF46689. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEQRVNSDV LTVSTVNSQD QVTQKPLRDS VKQALKNYFA QLNGQDVNDL
60 70 80 90
YELVLAEVEQ PLLDMVMQYT RGNQTRAALM MGINRGTLRK KLKKYGMN
Length:98
Mass (Da):11,240
Last modified:May 10, 2005 - v1
Checksum:i2E339BFCFCDB163C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03245 Genomic DNA. Translation: AAA83856.1.
J03816 Genomic DNA. Translation: AAA98812.1.
M95784 Genomic DNA. Translation: AAA23783.1.
U18997 Genomic DNA. Translation: AAA58065.1.
U00096 Genomic DNA. Translation: AAC76293.1.
AP009048 Genomic DNA. Translation: BAE77302.1.
PIRiA32142. DNECFS.
RefSeqiNP_417727.1. NC_000913.3.
WP_000462905.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76293; AAC76293; b3261.
BAE77302; BAE77302; BAE77302.
GeneIDi8911232.
947697.
KEGGiecj:JW3229.
eco:b3261.
PATRICi32121952. VBIEscCol129921_3360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03245 Genomic DNA. Translation: AAA83856.1.
J03816 Genomic DNA. Translation: AAA98812.1.
M95784 Genomic DNA. Translation: AAA23783.1.
U18997 Genomic DNA. Translation: AAA58065.1.
U00096 Genomic DNA. Translation: AAC76293.1.
AP009048 Genomic DNA. Translation: BAE77302.1.
PIRiA32142. DNECFS.
RefSeqiNP_417727.1. NC_000913.3.
WP_000462905.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ETKX-ray2.10A/B1-98[»]
1ETOX-ray1.90A/B1-98[»]
1ETQX-ray2.80A/B/C/D1-98[»]
1ETVX-ray2.00A/B1-98[»]
1ETWX-ray2.00A/B1-98[»]
1ETXX-ray1.90A/B1-98[»]
1ETYX-ray2.00A/B1-98[»]
1F36X-ray2.65A/B1-98[»]
1FIAX-ray2.00A/B1-98[»]
1FIPX-ray1.90A/B1-98[»]
3FISX-ray2.30A/B1-98[»]
3IV5X-ray2.90A/B1-98[»]
3JR9X-ray2.90A/B1-98[»]
3JRAX-ray3.11A/B1-98[»]
3JRBX-ray3.10A/B1-98[»]
3JRCX-ray3.08A/B1-98[»]
3JRDX-ray3.10A/B1-98[»]
3JREX-ray3.17A/B1-98[»]
3JRFX-ray3.05A/B1-98[»]
3JRGX-ray3.11A/B1-98[»]
3JRHX-ray2.88A/B1-98[»]
3JRIX-ray3.11A/B1-98[»]
4FISX-ray2.30A/B1-98[»]
5DTDX-ray2.64A/B1-98[»]
5E3LX-ray2.66A/B1-98[»]
5E3MX-ray2.89A/B1-98[»]
5E3NX-ray2.66A/B1-98[»]
5E3OX-ray2.78A/B1-98[»]
DisProtiDP00422.
ProteinModelPortaliP0A6R3.
SMRiP0A6R3. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261864. 53 interactions.
DIPiDIP-47975N.
IntActiP0A6R3. 25 interactions.
MINTiMINT-1225073.
STRINGi511145.b3261.

Proteomic databases

EPDiP0A6R3.
PaxDbiP0A6R3.
PRIDEiP0A6R3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76293; AAC76293; b3261.
BAE77302; BAE77302; BAE77302.
GeneIDi8911232.
947697.
KEGGiecj:JW3229.
eco:b3261.
PATRICi32121952. VBIEscCol129921_3360.

Organism-specific databases

EchoBASEiEB0313.
EcoGeneiEG10317. fis.

Phylogenomic databases

eggNOGiCOG2901. LUCA.
HOGENOMiHOG000256331.
InParanoidiP0A6R3.
KOiK03557.
OMAiMSDNANL.
PhylomeDBiP0A6R3.

Enzyme and pathway databases

BioCyciEcoCyc:PD00196.
ECOL316407:JW3229-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6R3.
PROiP0A6R3.

Gene expression databases

CollecTFiEXPREG_00000770.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
HAMAPiMF_00166. DNA_binding_Fis. 1 hit.
InterProiIPR005412. Fis_DNA-bd.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF002097. DNA-binding_Fis. 1 hit.
PRINTSiPR01591. DNABINDNGFIS.
PR01590. HTHFIS.
SUPFAMiSSF46689. SSF46689. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFIS_ECOLI
AccessioniPrimary (citable) accession number: P0A6R3
Secondary accession number(s): P11028, P37404, Q2M8V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.