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P0A6R3 (FIS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding protein Fis
Alternative name(s):
Factor-for-inversion stimulation protein
Hin recombinational enhancer-binding protein
Gene names
Name:fis
Ordered Locus Names:b3261, JW3229
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length98 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Ref.7 Ref.8

Subunit structure

Homodimer.

Sequence similarities

Belongs to the transcriptional regulatory Fis family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

yebVP645033EBI-550170,EBI-9126792

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9898DNA-binding protein Fis HAMAP-Rule MF_00166
PRO_0000203878

Regions

DNA binding74 – 9320H-T-H motif HAMAP-Rule MF_00166
Region17 – 4428Required for the stimulation of HIN-mediated recombination HAMAP-Rule MF_00166

Secondary structure

............... 98
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A6R3 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 2E339BFCFCDB163C

FASTA9811,240
        10         20         30         40         50         60 
MFEQRVNSDV LTVSTVNSQD QVTQKPLRDS VKQALKNYFA QLNGQDVNDL YELVLAEVEQ 

        70         80         90 
PLLDMVMQYT RGNQTRAALM MGINRGTLRK KLKKYGMN 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the gene encoding the Hin recombinational enhancer binding protein."
Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE.
[2]"Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene."
Koch C., Vanderkerckhove J., Kahmann R.
Proc. Natl. Acad. Sci. U.S.A. 85:4237-4241(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-29.
[3]"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis."
Green J., Anjum M.F., Guest J.R.
Mol. Microbiol. 20:1043-1055(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-11.
[7]"E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo."
Ross W., Thompson J.F., Newlands J.T., Gourse R.L.
EMBO J. 9:3733-3742(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro."
Wold S., Crooke E., Skarstad K.
Nucleic Acids Res. 24:3527-3532(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision."
Osuna R., Finkel S.E., Johnson R.C.
EMBO J. 10:1593-1603(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, DOMAINS.
[10]"The regulation of the Escherichia coli mazEF promoter involves an unusual alternating palindrome."
Marianovsky I., Aizenman E., Engelberg-Kulka H., Glaser G.
J. Biol. Chem. 276:5975-5984(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: BINDING TO THE MAZE-MAZF PROMOTER.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[11]"Three-dimensional structure of the E. coli DNA-binding protein FIS."
Kostrewa D., Granzin J., Koch C., Choe H.-W., Raghunathan S., Wolf W., Labahn J., Kahmann R., Saenger W.
Nature 349:178-180(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[12]"Crystal structure of the factor for inversion stimulation FIS at 2.0-A resolution."
Kostrewa D., Granzin J., Stock D., Choe H.-W., Labahn J., Saenger W.
J. Mol. Biol. 226:209-226(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[13]"The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms."
Safo M.K., Yang W.Z., Corselli L., Cramton S.E., Yuan H.S., Johnson R.C.
EMBO J. 16:6860-6873(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).
[14]"Structural analysis of the transcriptional activation region on fis: crystal structures of six fis mutants with different activation properties."
Cheng Y.-S., Yang W.-Z., Johnson R.C., Yuan H.S.
J. Mol. Biol. 302:1139-1151(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANTS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03245 Genomic DNA. Translation: AAA83856.1.
J03816 Genomic DNA. Translation: AAA98812.1.
M95784 Genomic DNA. Translation: AAA23783.1.
U18997 Genomic DNA. Translation: AAA58065.1.
U00096 Genomic DNA. Translation: AAC76293.1.
AP009048 Genomic DNA. Translation: BAE77302.1.
PIRDNECFS. A32142.
RefSeqNP_417727.1. NC_000913.3.
YP_491443.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ETKX-ray2.10A/B1-98[»]
1ETOX-ray1.90A/B1-98[»]
1ETQX-ray2.80A/B/C/D1-98[»]
1ETVX-ray2.00A/B1-98[»]
1ETWX-ray2.00A/B1-98[»]
1ETXX-ray1.90A/B1-98[»]
1ETYX-ray2.00A/B1-98[»]
1F36X-ray2.65A/B1-98[»]
1FIAX-ray2.00A/B1-98[»]
1FIPX-ray1.90A/B1-98[»]
3FISX-ray2.30A/B1-98[»]
3IV5X-ray2.90A/B1-98[»]
3JR9X-ray2.90A/B1-98[»]
3JRAX-ray3.11A/B1-98[»]
3JRBX-ray3.10A/B1-98[»]
3JRCX-ray3.08A/B1-98[»]
3JRDX-ray3.10A/B1-98[»]
3JREX-ray3.17A/B1-98[»]
3JRFX-ray3.05A/B1-98[»]
3JRGX-ray3.11A/B1-98[»]
3JRHX-ray2.88A/B1-98[»]
3JRIX-ray3.11A/B1-98[»]
4FISX-ray2.30A/B1-98[»]
DisProtDP00422.
ProteinModelPortalP0A6R3.
SMRP0A6R3. Positions 1-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47975N.
IntActP0A6R3. 25 interactions.
MINTMINT-1225073.
STRING511145.b3261.

Proteomic databases

PaxDbP0A6R3.
PRIDEP0A6R3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76293; AAC76293; b3261.
BAE77302; BAE77302; BAE77302.
GeneID12932870.
947697.
KEGGecj:Y75_p3179.
eco:b3261.
PATRIC32121952. VBIEscCol129921_3360.

Organism-specific databases

EchoBASEEB0313.
EcoGeneEG10317. fis.

Phylogenomic databases

eggNOGCOG2901.
HOGENOMHOG000256331.
KOK03557.
OMAMSDNANL.
OrthoDBEOG67HJXZ.
ProtClustDBPRK00430.

Enzyme and pathway databases

BioCycEcoCyc:PD00196.
ECOL316407:JW3229-MONOMER.

Gene expression databases

GenevestigatorP0A6R3.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
HAMAPMF_00166. DNA_binding_Fis.
InterProIPR005412. Fis_DNA-bd.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
[Graphical view]
PfamPF02954. HTH_8. 1 hit.
[Graphical view]
PIRSFPIRSF002097. DNA-binding_Fis. 1 hit.
PRINTSPR01591. DNABINDNGFIS.
PR01590. HTHFIS.
SUPFAMSSF46689. SSF46689. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A6R3.
PROP0A6R3.

Entry information

Entry nameFIS_ECOLI
AccessionPrimary (citable) accession number: P0A6R3
Secondary accession number(s): P11028, P37404, Q2M8V4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene