P0A6P7 (ENGB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable GTP-binding protein EngB

Gene names

Name:	engB
Synonyms:	yihA
Ordered Locus Names:	b3865, JW5930

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for normal cell division and for the maintenance of normal septation. Depletion of this protein leads to a severe reduction in growth rate and to extensive filamentation, with a block beyond the stage of segregation. Essential for bacteria survival. Ref.5
Sequence similarities	Belongs to the EngB family. Contains 1 G (guanine nucleotide-binding) domain.
Sequence caution	The sequence AAA24403.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB02999.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE77444.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Septation
Ligand	GTP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 210

210

Probable GTP-binding protein EngB HAMAP MF_00321

PRO_0000157747

Regions

Domain

38 – 146

109

G

Nucleotide binding

33 – 40

8

GTP Potential

Nucleotide binding

78 – 82

5

GTP Potential

Nucleotide binding

145 – 148

4

GTP Potential

Secondary structure

1

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210

Helix Strand Turn

Details...

Beta strand

12 – 17

6

Helix

18 – 20

3

Beta strand

26 – 33

8

Helix

39 – 43

5

Turn

44 – 46

3

Beta strand

65 – 71

7

Beta strand

74 – 78

5

Helix

92 – 105

14

Beta strand

109 – 117

9

Helix

124 – 135

12

Beta strand

140 – 145

6

Helix

147 – 149

3

Helix

152 – 166

15

Helix

167 – 169

3

Beta strand

173 – 177

5

Turn

180 – 183

4

Helix

186 – 197

12

Sequences

	Sequence		Length	Mass (Da)
	P0A6P7 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 11BCA2ED59220C0D	FASTA	210	23,561
	10 20 30 40 50 60 MTNLNYQQTH FVMSAPDIRH LPSDTGIEVA FAGRSNAGKS SALNTLTNQK SLARTSKTPG 70 80 90 100 110 120 RTQLINLFEV ADGKRLVDLP GYGYAEVPEE MKRKWQRALG EYLEKRQSLQ GLVVLMDIRH 130 140 150 160 170 180 PLKDLDQQMI EWAVDSNIAV LVLLTKADKL ASGARKAQLN MVREAVLAFN GDVQVETFSS 190 200 210 LKKQGVDKLR QKLDTWFSEM QPVEETQDGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two genes immediately downstream from the polA gene of Escherichia coli." Joyce C.M., Grindley N.D. J. Bacteriol. 152:1211-1219(1982) [PubMed: 6183253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A new essential gene of the 'minimal genome' affecting cell division." Dassain M., Leroy A., Colosetti L., Carole S., Bouche J.-P. Biochimie 81:889-895(1999) [PubMed: 10572302] [Abstract] Cited for: FUNCTION.
[6]	Loferer H. Submitted (MAR-1998) to UniProtKB Cited for: IDENTIFICATION OF START CODON.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01663 Genomic DNA. Translation: AAA24403.1. Different initiation.
L19201 Genomic DNA. Translation: AAB02999.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76862.3.
AP009048 Genomic DNA. Translation: BAE77444.1. Different initiation.

PIR

S40810.

RefSeq

NP_418301.3. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PUI	X-ray	2.00	A/B	1-210	[»]

ProteinModelPortal

P0A6P7.

SMR

P0A6P7. Positions 11-198.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48178N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000255; EBESCP00000000255; EBESCG00000000211.
EBESCT00000016911; EBESCP00000016202; EBESCG00000015970.

GeneID

948358.

GenomeReviews

Gene locus JW5930 in contig AP009048_GR.
Gene locus b3865 in contig U00096_GR.

KEGG

ecj:JW5930.
eco:b3865.

PATRIC

32123229. VBIEscCol129921_3975.

Organism-specific databases

EchoBASE

EB1188.

EcoGene

EG11203. engB.

Phylogenomic databases

eggNOG

COG0218.

GeneTree

EBGT00050000009490.

HOGENOM

HBG447097.

OMA

EWAVSAD.

PhylomeDB

P0A6P7.

ProtClustDB

PRK00454.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11203-MONOMER.

Gene expression databases

Genevestigator

P0A6P7.

Family and domain databases

HAMAP

MF_00321. GTPase_EngB.
[Tree]

InterPro

IPR019987. GTP-bd_ribosome_bio_YsxC.
IPR006073. GTP_binding_domain.
[Graphical view]

KO

K03978.

Pfam

PF01926. MMR_HSR1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03598. GTPase_YsxC. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ENGB_ECOLI

Accession

Primary (citable) accession number: P0A6P7
Secondary accession number(s): P24253, P76771, Q2M8G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program