##gff-version 3
P0A6P1	UniProtKB	Initiator methionine	1	1	.	.	.	Note=Removed;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:9298646,ECO:0000269|PubMed:9600841,ECO:0000269|Ref.7;Dbxref=PMID:9298646,PMID:9600841	
P0A6P1	UniProtKB	Chain	2	283	.	.	.	ID=PRO_0000161117;Note=Elongation factor Ts	
P0A6P1	UniProtKB	Region	80	83	.	.	.	Note=Involved in Mg(2+) ion dislocation from EF-Tu;Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00050	
P0A6P1	UniProtKB	Mutagenesis	24	24	.	.	.	Note=No change in binding to EF-Tu and in promoting GDP exchange. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9468511;Dbxref=PMID:9468511	
P0A6P1	UniProtKB	Mutagenesis	81	81	.	.	.	Note=2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF-Tu. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9468511;Dbxref=PMID:9468511	
P0A6P1	UniProtKB	Mutagenesis	82	82	.	.	.	Note=2 to 3-fold less active in promoting GDP exchange and 6-fold less active in binding to EF-Tu. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9468511;Dbxref=PMID:9468511	
P0A6P1	UniProtKB	Mutagenesis	148	148	.	.	.	Note=At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9468511;Dbxref=PMID:9468511	
P0A6P1	UniProtKB	Mutagenesis	186	225	.	.	.	Note=Resistant to toxins CdiA-CT-EC869%2C CdiA-CT-NC101 and CdiA-CT-96.154%2C but not other CdiA toxins in growth competition experiments%2C in vitro prevents CdiA-CT-EC869 from digesting tRNA(GUG-Gln). Resistant to bacteriophage R17. VSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKKFTG->EPGGEA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28223500;Dbxref=PMID:28223500	
P0A6P1	UniProtKB	Mutagenesis	188	227	.	.	.	Note=Still associates with EF-Tu%2C no longer forms the Qbeta viral RNA polymerase complex. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20798060;Dbxref=PMID:20798060	
P0A6P1	UniProtKB	Mutagenesis	202	202	.	.	.	Note=Resistant to toxin CdiA-CT-EC869%2C partially resistant to toxins CdiA-CT-NC101 and CdiA-CT-96.154%2C but not resistant to other CdiA toxins in growth competition experiments%2C in vitro prevents CdiA-CT-EC869 from digesting tRNA(GUG-Gln). Partially resistant to bacteriophage R17. A->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28223500;Dbxref=PMID:28223500	
P0A6P1	UniProtKB	Mutagenesis	219	219	.	.	.	Note=Resistant to toxin CdiA-CT-EC869%2C partially resistant to toxins CdiA-CT-NC101 and CdiA-CT-96.154%2C but not resistant to other CdiA toxins in growth competition experiments. Resistant to bacteriophage R17. R->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28223500;Dbxref=PMID:28223500	
P0A6P1	UniProtKB	Helix	6	16	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	20	29	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Turn	30	32	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	34	52	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	59	67	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	70	79	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	81	85	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	87	103	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	108	126	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	131	139	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	141	148	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Turn	149	151	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	152	160	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	163	176	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	179	182	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	183	185	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	188	204	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	209	226	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Turn	229	231	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	232	234	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1EFU	
P0A6P1	UniProtKB	Beta strand	237	241	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1EFU	
P0A6P1	UniProtKB	Helix	242	248	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Beta strand	252	260	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Turn	261	264	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3	
P0A6P1	UniProtKB	Helix	272	279	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4PC3