P0A6N4 (EFP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor P
Short name=EF-P

Gene names

Name:	efp
Ordered Locus Names:	b4147, JW4107

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in peptide bond synthesis, probably at an early stage of protein synthesis. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis. HAMAP MF_00141
Pathway	Protein biosynthesis; polypeptide chain elongation. HAMAP MF_00141
Subunit structure	Binds 30S, 50S and 70S ribosomes, possibly near the A site, note that T.thermophilus structures show binding between the P and E sites (PDB 3HUW and 3HUY). Proportionally less EF-P binds to larger polysomes, suggesting it has a role early in translation. It is present in 1 copy per 10 ribosomes.
Subcellular location	Cytoplasm HAMAP MF_00141.
Post-translational modification	Has been seen to be partially modified on residues 32-34 by an unidentified entity with a mass difference of 143.77 Da. HAMAP MF_00141
Disruption phenotype	Lethality. Ref.7
Sequence similarities	Belongs to the elongation factor P family.
Caution	The ptm may be an artifact.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Elongation factor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	peptide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: EcoCyc
Molecular function	ribosome binding Inferred from direct assay Ref.9. Source: EcoCyc translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.5
	Chain	2 – 188	187	Elongation factor P HAMAP MF_00141		PRO_0000094245

Sequences

	Sequence		Length	Mass (Da)
	P0A6N4 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E36E136D4399460F	FASTA	188	20,591
	10 20 30 40 50 60 MATYYSNDFR AGLKIMLDGE PYAVEASEFV KPGKGQAFAR VKLRRLLTGT RVEKTFKSTD 70 80 90 100 110 120 SAEGADVVDM NLTYLYNDGE FWHFMNNETF EQLSADAKAI GDNAKWLLDQ AECIVTLWNG 130 140 150 160 170 180 QPISVTPPNF VELEIVDTDP GLKGDTAGTG GKPATLSTGA VVKVPLFVQI GEVIKVDTRS GEYVSRVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis." Aoki H., Adams S.-L., Chung D.-G., Yaguchi M., Chuang S.-E., Ganoza M.C. Nucleic Acids Res. 19:6215-6220(1991) [PubMed: 1956781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-33; 71-80 AND 86-117.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13. Strain: K12 / EMG2.
[6]	"Molecular characterization of the prokaryotic efp gene product involved in a peptidyltransferase reaction." Aoki H., Adams S.-L., Turner M.A., Ganoza M.C. Biochimie 79:7-11(1997) [PubMed: 9195040] [Abstract] Cited for: CHARACTERIZATION.
[7]	"The gene encoding the elongation factor P protein is essential for viability and is required for protein synthesis." Aoki H., Dekany K., Adams S.L., Ganoza M.C. J. Biol. Chem. 272:32254-32259(1997) [PubMed: 9405429] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[8]	"Identification and quantitation of elongation factor EF-P in Escherichia coli cell-free extracts." An G., Glick B.R., Friesen J.D., Ganoza M.C. Can. J. Biochem. 58:1312-1314(1980) [PubMed: 7011506] [Abstract] Cited for: COPY NUMBER.
[9]	"Interactions of elongation factor EF-P with the Escherichia coli ribosome." Aoki H., Xu J., Emili A., Chosay J.G., Golshani A., Ganoza M.C. FEBS J. 275:671-681(2008) [PubMed: 18201202] [Abstract] Cited for: PUTATIVE PTM, RIBOSOME-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61676 Genomic DNA. Translation: CAA43851.1. U14003 Genomic DNA. Translation: AAA97046.1. U00096 Genomic DNA. Translation: AAC77107.1. AP009048 Genomic DNA. Translation: BAE78149.1.
PIR	S34443.
RefSeq	NP_418571.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A6N4.
SMR	P0A6N4. Positions 3-187.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31834N.
IntAct	P0A6N4. 1 interaction.
Proteomic databases
PRIDE	P0A6N4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001595; EBESCP00000001595; EBESCG00000001316. EBESCT00000016420; EBESCP00000015711; EBESCG00000015480.
GeneID	948661.
GenomeReviews	Gene locus JW4107 in contig AP009048_GR. Gene locus b4147 in contig U00096_GR.
KEGG	ecj:JW4107. eco:b4147.
PATRIC	32123863. VBIEscCol129921_4279.
Organism-specific databases
EchoBASE	EB2023.
EcoGene	EG12099. efp.
Phylogenomic databases
eggNOG	COG0231.
GeneTree	EBGT00050000010829.
HOGENOM	HBG303311.
OMA	KDFTFLY.
PhylomeDB	P0A6N4.
ProtClustDB	PRK00529.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12099-MONOMER.
Gene expression databases
Genevestigator	P0A6N4.
Family and domain databases
HAMAP	MF_00141. EF-P. [Tree]
InterPro	IPR015365. Elong-fact-P_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR020599. Transl_elong_fac_P/YeiP. IPR013185. Transl_elong_KOW-like. IPR001059. Transl_elong_P/YeiP_cen. IPR013852. Transl_elong_P/YeiP_CS. IPR011768. Transl_elongation_fac_P. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 2 hits. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
KO	K02356.
Pfam	PF01132. EFP. 1 hit. PF08207. EFP_N. 1 hit. PF09285. Elong-fact-P_C. 1 hit. [Graphical view]
PIRSF	PIRSF005901. EF-P. 1 hit.
SMART	SM00841. Elong-fact-P_C. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 2 hits. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR00038. Efp. 1 hit.
PROSITE	PS01275. EFP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFP_ECOLI

Accession

Primary (citable) accession number: P0A6N4
Secondary accession number(s): P33398, Q2M6F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program