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Reviewed, UniProtKB/Swiss-Prot P0A6N4 (EFP_ECOLI)

Last modified June 16, 2009. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Elongation factor P
      Short name=EF-P
Gene names
Name: efp
Ordered Locus Names: b4147, JW4107
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. HAMAP MF_00141

Pathway

Protein biosynthesis; polypeptide chain elongation. HAMAP MF_00141

Subcellular location

Cytoplasm. HAMAP MF_00141

Sequence similarities

Belongs to the elongation factor P family.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   Molecular functionElongation factor
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processtranslational elongation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontranslation elongation factor activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.5
Chain2 – 188187Elongation factor P HAMAP MF_00141
PRO_0000094245

Experimental info

Sequence conflict811F → P AA sequence Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P0A6N4-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: E36E136D4399460F

FASTA18820,591
        10         20         30         40         50         60 
MATYYSNDFR AGLKIMLDGE PYAVEASEFV KPGKGQAFAR VKLRRLLTGT RVEKTFKSTD 

        70         80         90        100        110        120 
SAEGADVVDM NLTYLYNDGE FWHFMNNETF EQLSADAKAI GDNAKWLLDQ AECIVTLWNG 

       130        140        150        160        170        180 
QPISVTPPNF VELEIVDTDP GLKGDTAGTG GKPATLSTGA VVKVPLFVQI GEVIKVDTRS 


GEYVSRVK

« Hide

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References

« Hide 'large scale' references
[1]"Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis."
Aoki H., Adams S.-L., Chung D.-G., Yaguchi M., Chuang S.-E., Ganoza M.C.
Nucleic Acids Res. 19:6215-6220(1991) [PubMed: 1956781] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-33; 71-80 AND 86-117.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13.
Strain: K12 / EMG2.
[6]"Molecular characterization of the prokaryotic efp gene product involved in a peptidyltransferase reaction."
Aoki H., Adams S.-L., Turner M.A., Ganoza M.C.
Biochimie 79:7-11(1997) [PubMed: 9195040] [Abstract]
Cited for: CHARACTERIZATION.

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Cross-references

Sequence databases

X61676 Genomic DNA. Translation: CAA43851.1.
U14003 Genomic DNA. Translation: AAA97046.1.
U00096 Genomic DNA. Translation: AAC77107.1.
AP009048 Genomic DNA. Translation: BAE78149.1.
PIRS34443.
RefSeqAP_004648.1.
NP_418571.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID948661.
GenomeReviewsGene locus JW4107 in contig AP009048_GR.
Gene locus b4147 in contig U00096_GR.
KEGGecj:JW4107.
eco:b4147.

Organism-specific databases

EchoBASEEB2023.
EcoGeneEG12099. efp.
CMRSearch...

Phylogenomic databases

HOGENOMP0A6N4.
OMAP0A6N4. ATIEDHN.

Enzyme and pathway databases

BioCycEcoCyc:EG12099-MON.

Family and domain databases

HAMAPMF_00141.
[Tree]
InterProIPR015365. Elong-fact-P_C.
IPR012340. NA-bd_OB-fold.
IPR011768. Transl_elong_EFP.
IPR013185. Transl_elong_KOW-like.
IPR001059. Transl_elong_P/YeiP_cen.
IPR013852. Transl_elong_P/YeiP_CS.
IPR014722. Transl_SH3-like_sub.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 2 hits.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
PfamPF01132. EFP. 1 hit.
PF08207. EFP_N. 1 hit.
PF09285. Elong-fact-P_C. 1 hit.
[Graphical view]
PIRSFPIRSF005901. EF-P. 1 hit.
TIGRFAMsTIGR00038. efp. 1 hit.
PROSITEPS01275. EFP. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameEFP_ECOLI
AccessionPrimary (citable) accession number: P0A6N4
Secondary accession number(s): P33398, Q2M6F7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents