Reviewed, UniProtKB/Swiss-Prot P0A6N3 (EFTU_ECO57)

Names and origin

Protein names

Recommended name:
    Elongation factor Tu
      Short name=EF-Tu
Alternative name(s):
    P-43

Gene names

Name:	tufA
Ordered Locus Names:	Z4697, ECs4190
AND
Name:	tufB
Ordered Locus Names:	Z5553, ECs4903

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. May play an important regulatory role in cell growth and in the bacterial response to nutrient deprivation By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein By similarity.
Miscellaneous	This chain is also used in bacteriophage Q-beta RNA polymerase By similarity. The antibiotic kirromycin inhibits protein biosynthesis by inhibiting the release of EF-Tu from the ribosome By similarity. The antibiotic pulvomycin inhibits protein biosynthesis by disrupting the allosteric control mechanism of EF-Tu By similarity. The sequence of the tufB gene is shown. TufA differs in a single position By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Protein biosynthesis
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translational elongation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 394	393	Elongation factor Tu HAMAP MF_00118		PRO_0000091321
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	57	1	N6-methylated lysine By similarity
	Modified residue	314	1	N6-acetyllysine By similarity
	Modified residue	383	1	Phosphothreonine By similarity
Natural variations
	Natural variant	394	1	S → G in tufA.

Sequences

	Sequence		Length	Mass (Da)
	P0A6N3-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6EDA60255F43358F	FASTA	394	43,314
	10 20 30 40 50 60 MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AITTVLAKTY GGAARAFDQI DNAPEEKARG 70 80 90 100 110 120 ITINTSHVEY DTPTRHYAHV DCPGHADYVK NMITGAAQMD GAILVVAATD GPMPQTREHI 130 140 150 160 170 180 LLGRQVGVPY IIVFLNKCDM VDDEELLELV EMEVRELLSQ YDFPGDDTPI VRGSALKALE 190 200 210 220 230 240 GDAEWEAKIL ELAGFLDSYI PEPERAIDKP FLLPIEDVFS ISGRGTVVTG RVERGIIKVG 250 260 270 280 290 300 EEVEIVGIKE TQKSTCTGVE MFRKLLDEGR AGENVGVLLR GIKREEIERG QVLAKPGTIK 310 320 330 340 350 360 PHTKFESEVY ILSKDEGGRH TPFFKGYRPQ FYFRTTDVTG TIELPEGVEM VMPGDNIKMV 370 380 390 VTLIHPIAMD DGLRFAIREG GRTVGAGVVA KVLS

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TUFA AND TUFB). Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TUFA AND TUFB). Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG58446.1. AE005174 Genomic DNA. Translation: AAG59176.1. BA000007 Genomic DNA. Translation: BAB37613.1. BA000007 Genomic DNA. Translation: BAB38326.1.
PIR	B85998. G91241.
RefSeq	NP_289886.1. NP_290611.1. NP_312217.1. NP_312930.1.
3D structure databases
SMR	P0A6N3. Positions 9-394.
ModBase	Search...
Proteomic databases
PRIDE	P0A6N3.
Genome annotation databases
GeneID	914959. 915957. 958801. 960150.
GenomeReviews	Gene locus Z4697 in contig AE005174_GR. Gene locus Z5553 in contig AE005174_GR. Gene locus ECs4190 in contig BA000007_GR. Gene locus ECs4903 in contig BA000007_GR.
KEGG	ece:Z4697. ece:Z5553. ecs:ECs4190. ecs:ECs4903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A6N3.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4190-MON. ECOL83334:ECS4903-MON.
Family and domain databases
HAMAP	MF_00118. [Tree]
InterPro	IPR000795. ProtSyn_GTP_bd. IPR005225. Small_GTP_bd. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF31. Transl_elong_EFTu/EF1A_bac/org. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_ECO57

Accession

Primary (citable) accession number: P0A6N3
Secondary accession number(s): O68929 , P02990, Q8X4S9, Q8XED3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)